Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress

BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M phase.Recent research has shown that CDC25C could be a potential therapeutic target for cancers,particularly for hepatocellular carcinoma(HCC).However,the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely understood.AIM To explore the impact of CDC25C on cell proliferation and apoptosis,as well as its regulatory mechanisms in HCC development.METHODS Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences(LV-CDC25C shRNA)to knock down CDC25C.Subsequently,a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in vivo.Cell proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays,respectively.The expression of endoplasmic reticulum(ER)stress-related molecules(glucose-regulated protein 78,X-box binding protein-1,and C/EBP homologous protein)was measured in both cells and subcutaneous xenografts using quantitative real-time PCR(qRT-PCR)and western blotting.Additionally,apoptosis was investigated using flow cytometry,qRT-PCR,and western blotting.RESULTS CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction.A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in mice.CDC25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response,ultimately promoting ER stress-induced apoptosis in HCC cells.CONCLUSION The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signaling pathway.

The Cumulative Method for Multiplication and Division

This paper provides a method of the process of computation called the cumulative method, it is based upon repeated cumulative process. The cumulative method is being adapted to the purposes of computation, particularly multiplication and division. The operations of multiplication and division are represented by algebraic formulas. An advantage of the method is that the cumulative process can be performed on decimal numbers. The present paper aims to establish a basic and useful formula valid for the two fundamental arithmetic operations of multiplication and division. The new cumulative method proved to be more flexible and made it possible to extend the multiplication and division based on repeated addition/subtraction to decimal numbers.

Time-sequential color code division multiplexing holographic display with metasurface

Color metasurface holograms are powerful and versatile platforms for modulating the amplitude,phase,polarization,and other properties of light at multiple operating wavelengths.However,the current color metasurface holography can only realize static manipulation.In this study,we propose and demonstrate a multiplexing metasurface technique combined with multiwavelength code-division multiplexing(CDM)to realize dynamic manipulation.Multicolor code references are utilized to record information within a single metasurface and increase the information capacity and security for anticracks.A total of 48 monochrome images consisting of pure color characters and multilevel color video frames were reconstructed in dual polarization channels of the birefringent metasurface to exhibit high information density,and a video was displayed via sequential illumination of the corresponding code patterns to verify the ability of dynamic manipulation.Our approach demonstrates significant application potential in optical data storage,optical encryption,multiwavelengthversatile diffractive optical elements,and stimulated emission depletion microscopy.

Performance of phase-matching quantum key distribution based on wavelength division multiplexing technology

Quantum key distribution(QKD) generates information-theoretical secure keys between two parties based on the physical laws of quantum mechanics. The phase-matching(PM) QKD protocol allows the key rate to break the quantum channel secret key capacity limit without quantum repeaters, and the security of the protocol is demonstrated by using equivalent entanglement. In this paper, the wavelength division multiplexing(WDM) technique is applied to the PM-QKD protocol considering the effect of crosstalk noise on the secret key rate. The performance of PM-QKD protocol based on WDM with the influence of adjacent classical channels and Raman scattering is analyzed by numerical simulations to maximize the total secret key rate of the QKD, providing a reference for future implementations of QKD based on WDM techniques.

Production evolution patterns and development stage division of waterflooding oilfields

The continuous growth of recoverable reserves in a waterflooding oilfield has a significant impact on the patterns of production evolution. A new production evolution model is established by improving the Weng Cycle model. With the new model, the statistical correspondence between the production decline stage and the reserve-production imbalance is clarified,and the correlation of water cut with the recovery percent of recoverable reserves is discussed, providing quantitative basis of reservoir engineering for dividing development stages of oilfield and defining mature oilfields. According to the statistics of oilfields in eastern China, the time point corresponding to the reserve-production balance coefficient dropping to less than 1dramatically is well correlated the beginning point of production decline, thus the time when the reserve-production balance coefficient drops dramatically can be taken as the initiation point of production decline stage. The research results show that the water cut and the recovery percent of recoverable reserves have a good statistical match in the high water cut stage, and it is more rational to take both the start point of production decline stage and the water cut of 90%(or the recovery percent of recoverable reserves of 80%) as the critical criteria for defining a mature oilfield. Five production evolution patterns can be summarized as follows: growth–peak plateau–stepped decline, growth–stepped stabilizing–stepped decline, growth–stepped stabilizing–rapid decline, growth–peak plateau–rapid decline, and growth–continuous decline.

The Epigenetic“Dance”of Cell Divisions

The tango of life begins with the very first cell division.New research reveals that to dance their way through development,dividing cells must pass along not just DNA but molecular“memories”that guide their destinies.When this handoff gets fumbled,the dance falls apart.

Improved PBFT Consensus Algorithm Based on Node Role Division

The PBFT (Practical Byzantine Fault Tolerance, PBFT) consensus algorithm, which addressed the issue of malicious nodes sending error messages to disrupt the system operation in distributed systems, was challenging to support massive network nodes, the common participation over all nodes in the consensus mechanism would lead to increased communication complexity, and the arbitrary selection of master nodes would also lead to inefficient consensus. This paper offered a PBFT consensus method (Role Division-based Practical Byzantine Fault Tolerance, RD-PBFT) to address the above problems based on node role division. First, the nodes in the system voted with each other to divide the high reputation group and low reputation group, and determined the starting reputation value of the nodes. Then, the mobile node in the group was divided into roles according to the high reputation value, and a total of three roles were divided into consensus node, backup node, and supervisory node to reduce the number of nodes involved in the consensus process and reduced the complexity of communication. In addition, an adaptive method was used to select the master nodes in the consensus process, and an integer value was introduced to ensure the unpredictability and equality of the master node selection. Experimentally, it was verified that the algorithm has lower communication complexity and better decentralization characteristics compared with the PBFT consensus algorithm, which improved the efficiency of consensus.

Residual Film Pollution in the Eighth Division of the Xinjiang Production and Construction Corps

This study investigated the residual film content and distribution at different soil depths in the Eighth Division of the Xinjiang Production and Construction Corps.Before spring plowing in 2019,representative fields in four areas(Anjihai,Shihezi suburbs,Mosuowan and Xiayedi)were selected for residual film collection.The average content of residual film in the Eighth Division was 104 kg/ha.The residual film content in the four areas decreased in the order Anjihai>Shihezi suburbs>Mosuowan>Xiayedi.The average amount of residual film collected from cotton fields was greater than that from corn fields.Residual film content in the cotton field at soil depths of 0~10 and 10~30 cm was higher than that in the corn field,whereas the residual film content at a 30–50 cm soil depth in the corn field was higher than that in the cotton field.The results showed that farmers do not consider the long-term benefits,the high cost and short time of recycling,and the easy recycling of surface residual film.The shallow that the higher content of residual film,the less water in the soil of cotton.The same time,the results showed that the quantity of residual film in cotton field had greater influence on cotton quality.

Soil Organic Carbon and Nitrogen Dynamics in Arabica Coffee Agroforestry Systems in the Noun Division, West Cameroon

Agroforestry systems (AFSs) offer viable solutions to climate change because of the below-ground biomass (BGB) that is maintained by the soil. Therefore, spatially explicit estimation of their BGB is crucial to account for emission reduction efforts. A study to assess soil organic carbon (SOC) and nitrogen dynamics in Arabica coffee agroforests was conducted in two subdivisions (Foumbot and Kouoptamo) of the Noun Division in western Cameroon. The methodological approach involved the collection of 150 soil samples taken at different depths: 0 - 10, 10 - 20 and 20 - 30 cm. Depending on the depth, the SOC stock is 27.93 ± 1.13 tC/ha at 10 cm depth, 22.37 ± 1.47 tC/ha at 20 cm and 20.79 ± 0.31 tC/ha at 30 cm. According to the age classes of the Arabica coffee systems (ACA), the C/N ratio in our study area averaged 26.94 ± 13.60 for the (5 - 20) year old systems in Foumbot and 60.64 ± 48.80 for the (20 - 35) year old systems in Kouoptamo. Depending on the depth, at 10 cm this ratio is higher in Kouoptamo than in Foumbot with a maximum value of 57 and 38 respectively for the two subdivisions. In view of the results obtained, it would be important to analyse the types of microorganisms responsible for the decomposition of organic matter which is linked to soil organic carbon.

A High Spectral Efficient Frequency-Domain Channel-Estimation Method for the Polarization-Division-Multiplexed CO-OFDM-OQAM System

Contrary to the other multi-carrier modulation systems, the coherent optical orthogonal frequency division multiplexing communication system with an offset quadrature amplitude modulation (CO-OFDM-OQAM) possesses inherent imaginary interference (IMI). This has an important impact on the channel estimation process. Currently, a variety of frequency-domain channel estimation methods have been proposed. However, there are various problems that still exist. For instance, in order to reduce the influence of IMI, it is necessary to insert more guard intervals between the training sequence and the payload, leading to the occupation of excessive spectrum resources. In order to address this problem, this work designs a high spectral efficient frequency-domain channel estimation method for the polarization-division-multiplexing CO-OFDM-OQAM systems. First, the working principle of the proposed method is described in detail. Then, its spectral efficiency, power peak-to-average ratio, and channel estimation performance are studied based on simulations. The simulation results show that the proposed method improves the spectral efficiency without worsening the power peak-to-average ratio. The channel estimation capability of this method is verified in three scenarios of long-distance transmissions, including back-to-back, 100 km, and 200 km transmissions. .

基于Division Mockup的卫星虚拟装配系统研究与开发

以虚拟装配软件Division Mockup为平台,提出了卫星虚拟装配系统的体系结构,并在Division Mockup软件下完成了卫星产品的装配过程规划。针对Division Mockup只偏重于装配过程图形仿真的特点,开发了后端的卫星装配工艺生成系统,并通过数据接口实现了和Division Mockup的集成,从而完成工序工步规划、工艺文档生成、装配现场示教等功能。最后给出了系统的应用实例。

基于Division Mockup装配仿真的实现方法

虚拟装配技术是CAD领域研究的关键技术之一。随着计算机软硬件技术的发展,虚拟装配在个人计算机上也能够实现。本文以大型面天线为例,介绍了在Division Mockup的软件环境下进行虚拟装配,并进行相应的干涉检验,截面分析等。

基于Division Mockup的分油机虚拟装配系统的研究

以Division Mockup软件为平台,进行了船用分油机的虚拟装配过程仿真。给出了虚拟装配系统的体系结构,介绍了不同CAD系统与Division Mockup软件之间的格式转换、交互式装配过程的进行,以及工艺文档生成等关键技术的研究。最终形成可进行装配验证和示教功能的分油机系统。

基于Division的军事电子装备虚拟现实技术

文章论述了虚拟现实技术在电子装备研制中的应用。首先介绍了VR的基本概念、国外的应用情况和软件Division的功能与模块;着重论述了在Division环境下开展电子装备研制的实现过程以及在雷达整机研制中VR的应用实践;总结了阶段性成果和下一阶段工作重点。

基于Division的TBM虚拟装配研究

讨论了盾构机虚拟装配系统的应用背景和实际需求,应用建模软件Pro/E及立体视觉驱动软件Division建立了基于虚拟现实技术的TBM虚拟装配系统,并探讨了这一技术路线的实现过程,以及在虚拟环境下的干涉检验等关键技术。实验结果表明,该系统在虚拟操作训练、虚拟施工等方面都有很好的应用前景。

Weber Law Based Approach for Multi-Class Image Forgery Detection

Today’s forensic science introduces a new research area for digital image analysis formultimedia security.So,Image authentication issues have been raised due to the wide use of image manipulation software to obtain an illegitimate benefit or createmisleading publicity by using tempered images.Exiting forgery detectionmethods can classify only one of the most widely used Copy-Move and splicing forgeries.However,an image can contain one or more types of forgeries.This study has proposed a hybridmethod for classifying Copy-Move and splicing images using texture information of images in the spatial domain.Firstly,images are divided into equal blocks to get scale-invariant features.Weber law has been used for getting texture features,and finally,XGBOOST is used to classify both Copy-Move and splicing forgery.The proposed method classified three types of forgeries,i.e.,splicing,Copy-Move,and healthy.Benchmarked(CASIA 2.0,MICCF200)and RCMFD datasets are used for training and testing.On average,the proposed method achieved 97.3% accuracy on benchmarked datasets and 98.3% on RCMFD datasets by applying 10-fold cross-validation,which is far better than existing methods.

Coarse-fine joint target parameter estimation method based on AN-RSC in OFDM passive radar

In this paper,we study the accuracy of delay-Doppler parameter estimation of targets in a passive radar using orthogonal frequency division multiplexing(OFDM)signal.A coarse-fine joint estimation method is proposed to achieve better estimation accuracy of target parameters without excessive computational burden.Firstly,the modulation symbol domain(MSD)method is used to roughly estimate the delay and Doppler of targets.Then,to obtain high-precision Doppler estimation,the atomic norm(AN)based on the multiple measurement vectors(MMV)model(MMV-AN)is used to manifest the signal sparsity in the continuous Doppler domain.At the same time,a reference signal compensation(RSC)method is presented to obtain highprecision delay estimation.Simulation results based on the OFDM signal show that the coarse-fine joint estimation method based on AN-RSC can obtain a more accurate estimation of target parameters compared with other algorithms.In addition,the proposed method also possesses computational advantages compared with the joint parameter estimation.

Turbo Message Passing Based Burst Interference Cancellation for Data Detection in Massive MIMO-OFDM Systems

This paper investigates the fundamental data detection problem with burst interference in massive multiple-input multiple-output orthogonal frequency division multiplexing(MIMO-OFDM) systems. In particular, burst interference may occur only on data symbols but not on pilot symbols, which means that interference information cannot be premeasured. To cancel the burst interference, we first revisit the uplink multi-user system and develop a matrixform system model, where the covariance pattern and the low-rank property of the interference matrix is discussed. Then, we propose a turbo message passing based burst interference cancellation(TMP-BIC) algorithm to solve the data detection problem, where the constellation information of target data is fully exploited to refine its estimate. Furthermore, in the TMP-BIC algorithm, we design one module to cope with the interference matrix by exploiting its lowrank property. Numerical results demonstrate that the proposed algorithm can effectively mitigate the adverse effects of burst interference and approach the interference-free bound.

江苏宿迁220kV变电站隔震设计方法研究

为解决变电站抗侧刚度较小,大型设备会放大地震作用的问题,对宿迁220kV变电站结构分别采用分部设计和一体化设计两种方法进行隔震设计,对支座参数确定原则、地震波选取规则、楼层响应以及非比例阻尼影响规律进行了研究分析。结果表明,应采用实际地震作用下迭代得到的支座参数,以迭代得到的隔震层阻尼比作为结构阻尼比选取地震波更合理,一体化设计方法可以在实现中震弹性目标要求的同时将构件进行优化,提高结构经济性,变电站隔震结构非比例阻尼特性较强,且对其楼层响应结果影响较大,实际隔震设计中不可忽略非比例阻尼影响,高烈度地区的变电站更宜采用一体化设计方法。

Polar codes based OFDM-PLC systems in the presence of impulsive noise

Power line communication(PLC)has the potential to become the preferred technique for providing broadband communication to homes and offices with advantage of eliminating the need for new wiring infrastructure and reducing the cost.But it suffers from the impulsive noise because it introduces significant time variance into the power line channel.In this paper,a polar codes based orthogonal frequency division multiplexing(OFDM)PLC system is proposed to deal with the impulsive noise and thereby improve the transmission performance.Firstly,the impulsive noise is modelled with a multi-damped sine function by analyzing the time behavior of impulse events.Then the polar codes are used to combat the impulsive noise of PLC channel,and a low complexity bit-flipping decoding method based on CRC-aided successive cancellation list(CA-SCL)decoding algorithm is proposed.Simulations evaluate the proposed decoding algorithm and the results validate the suggested polar codes based OFDM-PLC scheme which can improve the BER performance of PLC with impulsive interference.