AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahep...AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahepatic inferior vena cava,portal vein,hepatic artery or common bile duct.the operation was similar to the orthotopic liver transplantation except vascular anastomoses,the dog liver underwent the warm or cold ischemia and the reperfusated injurous process.RESULTS: The imitability was exactly good and the operation was simple and safe. Because the hepatic vessels of the going out or coming in was clamped block and might open or blind the blood flow whenever necessary,the model might control the warm or cold ischemic time accurately,and eliminate the influence or the complications due to vascular anastomoses.CONCLUSIONS: The model avoided many-sided Influences of the traditional OLT and was a good method to study hepatic artery or portal vein ischemic injury and created a new way to explore the pathogenesis or some complications in the OLT.展开更多
Objective To study the theraputic effect of extracorporeal liver perfusion on the treatment of acute liver failure. Methods Mongrel dogs weighing 12-14?kg were selected. Hepatic failure was induced by an end to si...Objective To study the theraputic effect of extracorporeal liver perfusion on the treatment of acute liver failure. Methods Mongrel dogs weighing 12-14?kg were selected. Hepatic failure was induced by an end to side portacaval shunt. The common hepatic and gastroduodenal arteries were occluded for 2 hours. To the control group ( n =7), the dogs received standard medical therapy . To the treating group ( n =10), the dogs received extracorporeal kidney and liver perfusion at the onset of the occlusion of the hepatic artery. During the liver support, the animals were frequently monitored regarding their clinical state, liver function, biochemical and hematological parameters. Results After the occlusion of the liver blood flow, all dogs died within 3-7.5 hours. The average survival time was (5.7±1.2) hours. Serum levels of ALT, AST, LDH and ammonia increased significantly. In the treating group, the dogs died within 7-10.5 hours. The average survival time was 8.6±1.1 hours. There were no significant diferences in serum levels of ALT, AST, LDH between the two groups( P >0.05). There were dramatic diferences in blood Ammonia level, PT, FIB between the two groups( P <0.05). The survival time was longer in treating group. The animals' blood pressure were more stable in the treating group than that in the control group. Conclusion The modified xenogenic liver perfusion can provide necessary hepatic function for the acute liver failure dogs.展开更多
文摘AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahepatic inferior vena cava,portal vein,hepatic artery or common bile duct.the operation was similar to the orthotopic liver transplantation except vascular anastomoses,the dog liver underwent the warm or cold ischemia and the reperfusated injurous process.RESULTS: The imitability was exactly good and the operation was simple and safe. Because the hepatic vessels of the going out or coming in was clamped block and might open or blind the blood flow whenever necessary,the model might control the warm or cold ischemic time accurately,and eliminate the influence or the complications due to vascular anastomoses.CONCLUSIONS: The model avoided many-sided Influences of the traditional OLT and was a good method to study hepatic artery or portal vein ischemic injury and created a new way to explore the pathogenesis or some complications in the OLT.
文摘Objective To study the theraputic effect of extracorporeal liver perfusion on the treatment of acute liver failure. Methods Mongrel dogs weighing 12-14?kg were selected. Hepatic failure was induced by an end to side portacaval shunt. The common hepatic and gastroduodenal arteries were occluded for 2 hours. To the control group ( n =7), the dogs received standard medical therapy . To the treating group ( n =10), the dogs received extracorporeal kidney and liver perfusion at the onset of the occlusion of the hepatic artery. During the liver support, the animals were frequently monitored regarding their clinical state, liver function, biochemical and hematological parameters. Results After the occlusion of the liver blood flow, all dogs died within 3-7.5 hours. The average survival time was (5.7±1.2) hours. Serum levels of ALT, AST, LDH and ammonia increased significantly. In the treating group, the dogs died within 7-10.5 hours. The average survival time was 8.6±1.1 hours. There were no significant diferences in serum levels of ALT, AST, LDH between the two groups( P >0.05). There were dramatic diferences in blood Ammonia level, PT, FIB between the two groups( P <0.05). The survival time was longer in treating group. The animals' blood pressure were more stable in the treating group than that in the control group. Conclusion The modified xenogenic liver perfusion can provide necessary hepatic function for the acute liver failure dogs.
