Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determin...Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determine a suitable method to promptly detect MBL-producing gram-negative bacteria. Methods: A total of 103 gram-negative bacteria were identified from various clinical samples at a tertiary care hospital in Dhaka city. MBL producers were detected by two phenotypic methods, the Disk Potentiation Test (DPT) and the Double Disk Synergy Test (DDST) based on β-lactam chelator combinations where EDTA/SMA has been used as an inhibitor and Imipenem, Ceftazidime as substrates. Results: 103 isolates which were identified as Escherichia coli spp, Klebsiella spp, Pseudomonas spp, Acinetobacter spp, Proteus spp, Providencia spp were found to be multidrug-resistant in antibiogram test. Isolates showed complete resistance (100%) to Imipenem, Meropenem, and Amoxiclav. The highest carbapenem-resistant etiological agents were Acinetobacter spp 40 (38.8%) followed by Pseudomonas spp 27 (26.2%), Klebsiella spp 26 (25.2%), Escherichia coli 8 (7.8%), Proteus spp 1 (1%) and Providencia spp 1 (1%). DPT method detected significantly (p = 0.000009) a higher number of MBL-producers (Imipenem with 0.5 M EDTA n = 61, 59.2% & Ceftazidime with 0.5 M EDTA n = 56, 54.4%) compared to the DDST method (Imipenem -0.5 M EDTA n = 43, 41.7%, Imipenem – SMA n = 38, 36.9% & Ceftazidime -0.5 M EDTA n = 15, 14.6%). Conclusion: Pieces of evidence suggest that DPT is a more sensitive method than DDST and could be recommended for identifying MBL-producing bacteria in Bangladeshi hospitals for the proper management of patients, to reduce time constraints and treatment costs.展开更多
Rotating disk subjected to stationary slider loading system is a very common mechanical structure. This paper investigates the multibody dynamics of a rotating flexible annular thin disk subjected to double slider loa...Rotating disk subjected to stationary slider loading system is a very common mechanical structure. This paper investigates the multibody dynamics of a rotating flexible annular thin disk subjected to double slider loading systems. Along the rotating disk radial and circumferential directions, two stationary slider loading systems are distributed. System dynamic model is solved by Galerkin＇s method, and then natural frequency, dynamic stability and mode shape are determined with a quadratic eigenvalue problem. Effects of the distributing positions and interaction mechanism of the double slider loading systems on natural frequency, dynamic stability and mode shape are discussed and investigated.展开更多
In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the bas...In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the base fluid and Fe_(3)O_(4)+TiO_(2)as the hybrid nanofluid is considered.Several shapes of Fe_(3)O_(4)+TiO_(2)hybrid nanofluids,including sphere,brick,blade,cylinder,and platelet,are studied.Every shape exists in the same volume of a nanoparticle.The leading equations(partial differential equations(PDEs))are transformed to the nonlinear ordinary differential equations(ODEs)with the help of similarity transformations.The system of equations takes the form of ODEs depending on the boundary conditions,whose solutions are computed numerically by the bvp4c MATLAB solver.The outputs are compared with the previous findings,and an intriguing pattern is discovered,such that the tangential velocity is increased for the rotation parameter,while it is decreased by the stretching values because of the lower disk.For the reaction rate parameter,the concentration boundary layer becomes shorter,and the activation energy component increases the rate at which mass transfers come to the higher disk but have the opposite effect on the bottom disk.The ranges of various parameters taken into account are Pr=6.2,Re=2,M=1.0,φ_(1)=φ_(2)=0.03,K=0.5,S=-0.1,Br=0.3,Sc=2.0,α_(1)=0.2,γ=0.1,E_(n)=2.0,and q=1.0,and the rotation factor K is within the range of 0 to 1.展开更多
为了实现波浪能的采集与高效转换,加快波浪能开发利用,提出一种新型双盘磁齿整流发电机(double disk magnetic tooth rectifier generator,DDMTRG)。首先,介绍发电机的结构特点和整流原理,结合盘式转子和磁齿轮工作特点分析其结构参数;...为了实现波浪能的采集与高效转换,加快波浪能开发利用,提出一种新型双盘磁齿整流发电机(double disk magnetic tooth rectifier generator,DDMTRG)。首先,介绍发电机的结构特点和整流原理,结合盘式转子和磁齿轮工作特点分析其结构参数;其次,采用气隙比磁导法及有限元仿真分析该发电机的空载电磁特性和负载输出特性;第三,基于矩角特性分析磁齿轮的带载能力,由外定子各典型位置磁密特征解析该发电机铜耗和铁耗;最后,讨论外定子、盘式转子和磁齿轮结构参数对该发电机运行特性的影响规律。研究结果表明:该电机可将浮子的上下浮动转换为发电机单一方向的旋转运动,实现了波浪运动的整流,效率在94%左右,能量利用率较高;空载气隙磁密和电动势波形正弦性较优,计算结果基本吻合;磁齿轮结构参数对该发电机运行特性影响较大,当盘式转子外圆周永磁体极弧系数为0.6,厚度为4.5 mm,气隙长度为0.5 mm,轴向长度为100 mm,磁齿轮半径为35 mm,长度为40 mm,磁齿轮外圆周永磁体厚度为4 mm,极弧系数为0.8时,双盘磁齿整流发电机的性能较优,发电效率较高,磁齿轮带载能力较强。展开更多
文摘Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determine a suitable method to promptly detect MBL-producing gram-negative bacteria. Methods: A total of 103 gram-negative bacteria were identified from various clinical samples at a tertiary care hospital in Dhaka city. MBL producers were detected by two phenotypic methods, the Disk Potentiation Test (DPT) and the Double Disk Synergy Test (DDST) based on β-lactam chelator combinations where EDTA/SMA has been used as an inhibitor and Imipenem, Ceftazidime as substrates. Results: 103 isolates which were identified as Escherichia coli spp, Klebsiella spp, Pseudomonas spp, Acinetobacter spp, Proteus spp, Providencia spp were found to be multidrug-resistant in antibiogram test. Isolates showed complete resistance (100%) to Imipenem, Meropenem, and Amoxiclav. The highest carbapenem-resistant etiological agents were Acinetobacter spp 40 (38.8%) followed by Pseudomonas spp 27 (26.2%), Klebsiella spp 26 (25.2%), Escherichia coli 8 (7.8%), Proteus spp 1 (1%) and Providencia spp 1 (1%). DPT method detected significantly (p = 0.000009) a higher number of MBL-producers (Imipenem with 0.5 M EDTA n = 61, 59.2% & Ceftazidime with 0.5 M EDTA n = 56, 54.4%) compared to the DDST method (Imipenem -0.5 M EDTA n = 43, 41.7%, Imipenem – SMA n = 38, 36.9% & Ceftazidime -0.5 M EDTA n = 15, 14.6%). Conclusion: Pieces of evidence suggest that DPT is a more sensitive method than DDST and could be recommended for identifying MBL-producing bacteria in Bangladeshi hospitals for the proper management of patients, to reduce time constraints and treatment costs.
基金supported by the National Natural Science Foundation of China (51105164)
文摘Rotating disk subjected to stationary slider loading system is a very common mechanical structure. This paper investigates the multibody dynamics of a rotating flexible annular thin disk subjected to double slider loading systems. Along the rotating disk radial and circumferential directions, two stationary slider loading systems are distributed. System dynamic model is solved by Galerkin＇s method, and then natural frequency, dynamic stability and mode shape are determined with a quadratic eigenvalue problem. Effects of the distributing positions and interaction mechanism of the double slider loading systems on natural frequency, dynamic stability and mode shape are discussed and investigated.
文摘In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the base fluid and Fe_(3)O_(4)+TiO_(2)as the hybrid nanofluid is considered.Several shapes of Fe_(3)O_(4)+TiO_(2)hybrid nanofluids,including sphere,brick,blade,cylinder,and platelet,are studied.Every shape exists in the same volume of a nanoparticle.The leading equations(partial differential equations(PDEs))are transformed to the nonlinear ordinary differential equations(ODEs)with the help of similarity transformations.The system of equations takes the form of ODEs depending on the boundary conditions,whose solutions are computed numerically by the bvp4c MATLAB solver.The outputs are compared with the previous findings,and an intriguing pattern is discovered,such that the tangential velocity is increased for the rotation parameter,while it is decreased by the stretching values because of the lower disk.For the reaction rate parameter,the concentration boundary layer becomes shorter,and the activation energy component increases the rate at which mass transfers come to the higher disk but have the opposite effect on the bottom disk.The ranges of various parameters taken into account are Pr=6.2,Re=2,M=1.0,φ_(1)=φ_(2)=0.03,K=0.5,S=-0.1,Br=0.3,Sc=2.0,α_(1)=0.2,γ=0.1,E_(n)=2.0,and q=1.0,and the rotation factor K is within the range of 0 to 1.
