We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino ...We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino graft was implanted during each stage. During the firststage, an auxiliary domino liver transplantation was conducted using a domino graft from a 4-year-old female child with Wilson's disease. After removing the right lobe of the FAP patient's liver, the graft was rotated 90 degrees counterclockwise and placed along the right side of the inferior vena cava(IVC). The orifices of the left, middle, and right hepatic veins were reconstructed using an iliac vein patch and then anastomosed to the right side of the IVC. Thirty days later, a second domino liver graft was implanted. The second domino graft was from a 3-yearold female child with an ornithine carbamyl enzyme defect, and it replaced the residual native liver(left lobe). To balance the function and blood flow between the two grafts, a percutaneous transcatheter selective portal vein embolization was performed, and "the left portal vein" of the first graft was blocked 9 mo after the second transplantation. The liver function indices, blood ammonia, and 24-h urinary copper levels were normal at the end of a 3-year follow-up. These two domino donor grafts from donors with different metabolic disorders restored normal liver function. Our experience demonstrated a new approach for resolving metabolic disorders with domino grafts and utilizing explanted livers from children.展开更多
A 53-year-old man with a restenotic large right coronary vein graft was successfully treated by double balloon angioplasty. The technique and clinical application of double balloon angioplasty were discussed.
This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free mediu...This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts. Twelve weeks after transplantation, 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer DiI into the striatum results in fluorescent labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.展开更多
基金Supported by Capital Special Program for Health Research and Development,No.2016-1-2021National Key Technologies R&D Program,No.2015BAI13B09+1 种基金The Training Program of Academic Leaders in Beijing Health System,No.2014-2-002Beijing Municipal Administration of Hospitals Ascent Plan,No.DFL20150101
文摘We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino graft was implanted during each stage. During the firststage, an auxiliary domino liver transplantation was conducted using a domino graft from a 4-year-old female child with Wilson's disease. After removing the right lobe of the FAP patient's liver, the graft was rotated 90 degrees counterclockwise and placed along the right side of the inferior vena cava(IVC). The orifices of the left, middle, and right hepatic veins were reconstructed using an iliac vein patch and then anastomosed to the right side of the IVC. Thirty days later, a second domino liver graft was implanted. The second domino graft was from a 3-yearold female child with an ornithine carbamyl enzyme defect, and it replaced the residual native liver(left lobe). To balance the function and blood flow between the two grafts, a percutaneous transcatheter selective portal vein embolization was performed, and "the left portal vein" of the first graft was blocked 9 mo after the second transplantation. The liver function indices, blood ammonia, and 24-h urinary copper levels were normal at the end of a 3-year follow-up. These two domino donor grafts from donors with different metabolic disorders restored normal liver function. Our experience demonstrated a new approach for resolving metabolic disorders with domino grafts and utilizing explanted livers from children.
文摘A 53-year-old man with a restenotic large right coronary vein graft was successfully treated by double balloon angioplasty. The technique and clinical application of double balloon angioplasty were discussed.
基金Supported by the National Natural Science Foundationof China (No. 30 0 70 2 4 5 )
文摘This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts. Twelve weeks after transplantation, 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer DiI into the striatum results in fluorescent labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.
