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On Double Vector Bundles
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作者 Zhuo CHEN Zhang Ju LIU Yun He SHENG 《Acta Mathematica Sinica,English Series》 SCIE CSCD 2014年第10期1655-1673,共19页
In this paper, we construct a category of short exact sequences of vector bundles and prove that it is equivalent to the category of double vector bundles. Moreover, operations on double vector bundles can be transfer... In this paper, we construct a category of short exact sequences of vector bundles and prove that it is equivalent to the category of double vector bundles. Moreover, operations on double vector bundles can be transferred to operations on the corresponding short exact sequences. In particular, we study the duality theory of double vector bundles in term of the corresponding short exact sequences. Examples including the jet bundle and the Atiyah algebroid are discussed. 展开更多
关键词 double vector bundles DVB sequences DUALITY jet bundles
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Generating Marker-Free Transgenic Tobacco Plants by Agrobacteriummediated Transformation with Double T-DNA Binary Vector 被引量:6
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作者 周红艳 陈松彪 +3 位作者 李旭刚 肖桂芳 魏晓丽 朱祯 《Acta Botanica Sinica》 CSCD 2003年第9期1103-1108,共6页
We have developed a 'double T-DNA' binary vector system for generating selectable marker-free transgenic plants by Agrobacterium-mediated transformation. The 'double T-DNA' binary vector pDLBRBbarm whi... We have developed a 'double T-DNA' binary vector system for generating selectable marker-free transgenic plants by Agrobacterium-mediated transformation. The 'double T-DNA' binary vector pDLBRBbarm which carried two independent T-DNAs, one containing a selectable marker neomycin phosphotransferase (nptII) gene and the other a bargene, was constructed. Transgenic tobacco (Nicotiana tabacum L.) plants were then produced by Agrobacterium-mediated transformation with this vector. Frequency of the primary transformants co-integrated with npt II gene and bar gene was 59.2%. Segregation of two T-DNA regions was found in 3 out of 4 T-1 lines from co-transformed T-0 plants with nptII and bar PPT-resistant and kanamycin-sensitive plants were in approximate 19.5% of the T-1 plants. The result indicated that this 'double T-DNA' vector system could be a workable approach to generate transgenic plants free from selectable marker genes. Co-transformation of nptII gene and bar gene to plants with mixtures of Agrobacterium tumefaciens strains containing single T-DNA vectors was also tested. Frequency of co-transformed plants was 20.0%-47.7% and relatively low as compared with that of 'double T-DNA' vector system. 展开更多
关键词 plant transformation MARKER-FREE double T-DNA vector TOBACCO
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Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Bacterial Leaf Streak of Rice
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作者 Xu Mei-rong XIA Zhi-hui +3 位作者 ZHAI Wen-xue Xu Jian-long ZHOU Yong-li LI Zhi-kang 《Rice science》 SCIE 2008年第3期243-246,共4页
Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with ... Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxol, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation. 展开更多
关键词 Rxo1 gene double right-border binary vector RICE bacterial leaf streak selectable marker-free plant resistance gene
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Rapid Generation of Selectable Marker-Free Transgenic Rice with Three Target Genes by Co-Transformation and Anther Culture 被引量:6
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作者 ZHU Li FU Ya-ping +4 位作者 LIU Wen-zhen HU Guo-cheng SI Hua-min TANG Ke-xuan SUN Zong-xiu 《Rice science》 SCIE 2007年第4期239-246,共8页
The 'double T-DNA' binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene (hpf) in one T-DNA region and three target genes (hLF, SB401, RZ10) in another T-DNA r... The 'double T-DNA' binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene (hpf) in one T-DNA region and three target genes (hLF, SB401, RZ10) in another T-DNA region, was used to generate selectable marker-free transgenic rice by Agrobacterium-mediated transformation. The regenerated plants with both the three target genes and the selectable marker gene hpt were selected for anther culture. RT-PCR analysis indicated that target genes were inserted in rice genomic DNA and successfully transcribed. It took only one year to obtain double haploid selectable marker-free transgenic plants containing the three target genes with co-transformation followed by anther culture technique, and the efficiency was 12.2%. It was also noted that one or two target genes derived from the binary vector were lost in some transgenic rice plants. 展开更多
关键词 anther culture CO-TRANSFORMATION selectable marker-free transgenic plants RICE double T-DNA binary vector
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Development of Super-Synchronization Speed Control Assembly in Two 80 MVA Motor Generators of HL-2A Tokamak
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作者 LI Huajun DU Chang +9 位作者 XUAN Weimin PEN Jianfei HU Haotian LIU Lin KANG Li XU Lirong HUANG Zhaorong WANG Fen WANG Xiaoping WANG Haibing 《Southwestern Institute of Physics Annual Report》 2005年第1期71-73,共3页
The power of the toroidal field (TF) coil in HL-2A is supplied with two 80 MVA motor generators (MGs) together through a diode rectifier. The TF system is designed to obtain the parameters as follows: the highest... The power of the toroidal field (TF) coil in HL-2A is supplied with two 80 MVA motor generators (MGs) together through a diode rectifier. The TF system is designed to obtain the parameters as follows: the highest toroidal filed of 2.8 T, the biggest coil current of 45 kA, the fiat top time of 3-5 s. So the released energy of each MG set for one pulse discharge mast be more than 500 MJ. 展开更多
关键词 double fed drive motor AC-AC cycloconverte vector control
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