Dried blood spots,valid screening for viral hepatitis and human immunodeficiency virus in real-life

AIM To detect chronic hepatitis B(CHB),chronic hepatitis C(CHC) and human immunodeficiency virus(HIV) infections in dried blood spot(DBS) and compare these samples to venous blood sampling in real-life.METHODS We included prospective patients with known viral infections from drug treatment centers,a prison and outpatient clinics and included blood donors as negative controls. Five drops of finger capillary blood were spotted on filter paper,and a venous blood sample was obtained. The samples were analyzed for HBs Ag,antiHBc,anti-HBs,anti-HCV,and anti-HIV levels as well as subjected to a combined nucleic acid test(NAT) for HBV DNA,HCV RNA and HIV RNA.RESULTS Samples from 404 subjects were screened(85 CHB,116 CHC,114 HIV and 99 blood donors). DBS had a sensitivity of > 96% and a specificity of > 98% for the detection of all three infections. NAT testing did not improve sensitivity,but correctly classified 95% of the anti-HCV-positive patients with chronic and past infections. Anti-HBc and anti-HBS showed low sensitivity in DBS(68% and 42%).CONCLUSION DBS sampling,combined with an automated analysis system,is a feasible screening method to diagnose chronic viral hepatitis and HIV infections outside of the health care system.

Efficacy of recombinant human epidermal growth factor plus sodium hyaluronate eye drops in diabetic dry eye post-cataract surgery

BACKGROUND Dry eye syndrome(DES)after diabetic cataract surgery can seriously affect the patient’s quality of life.Therefore,effective alleviation of symptoms in patients with this disease has important clinical significance.AIM To explore the clinical effect of recombinant human epidermal growth factor(rhEGF)plus sodium hyaluronate(SH)eye drops on DES after cataract surgery in patients with diabetes.METHODS We retrospectively evaluated 82 patients with diabetes who experienced DES after cataract surgery at Tianjin Beichen Hospital,Affiliated Hospital of Nankai University between April 2021 and April 2023.They were classified into an observation group(42 cases,rhEGF+SH eye drops)and a control group(40 cases,SH eye drops alone),depending on the different treatment schemes.The therapeutic efficacy,dry eye symptom score,tear film breakup time(TFBUT),basic tear secretion score[assessed using Schirmer I test(SIt)],corneal fluorescein staining(FL)score,tear inflammatory markers,adverse reactions during treat-ment,and treatment satisfaction were compared between the two groups.RESULTS Therapeutic efficacy was higher in the observation group compared with the control group.Both groups showed improved TFBUT and dry eye,as well as improved SIt and FL scores after treatment,with a more pronounced improvement in the observation group.Although no marked differences in adverse reactions were observed between the two groups,treatment satisfaction was higher in the observation group.CONCLUSION rhEGF+SH eye drops rendered clinical benefits to patients by effectively ameliorating dry eye and visual impairment with favorable efficacy,fewer adverse reactions,and high safety levels.Thus,this treatment should be promoted in clinical practice.

Field Utilization of Dried Water Hyacinth for Phosphorous Recovery from Source-Separated Human Urine

This research demonstrated the feasibility of converting source-separated human urine into a solid fertilizer by means of continuous absorption and solar thermal evaporation using dried water hyacinth as adsorbent. In a preliminary experiment, the dried petioles of water hyacinth (DWH) absorbed urine in a mean rate of 18.78 ml·g-1 within 7 d, retrieving about 3.46% urine dissolved solids (UDS). In an advanced experiment, the DWH’s capacity of urine absorption declined from an initial 2.73 L·kg-1·d-1 to 0.68 L·kg-1·d-1, with a requirement of material change in about 25 effective days and an average ratio of 25 (L) to 1 (kg). Phosphorus (P2O5) concentration in the adsorbent increased from 0.46% (material baseline) to 3.14% (end product), suggesting a satisfactory recovery of the element. In field application, the urine was discharged, not in wet weather, onto the DWH via a tube connected to a waterless urinal. There are several ways to use the UDS-DWH as P(K)-rich fertilizer, e.g., making soluble fertilizer for foliage spraying to encourage prolific flowering and fruiting. Apparently, utilization of water hyacinth waste to recover dissolved plant nutrient elements from source-separated urine will benefit the environment in a wide range of perspectives. The herein innovative use of water hyacinth is also expected to be useful in the recycling of certain dissolved hazardous materials.

Clinical, Hematological and Biochemical Health Benefit Effects of Hibiscus sabdariffa Lin Dried Calyces Beverage in Human

Background/Objective: This study was carried out to investigate the health benefit effects of Hibiscus sabdariffa Lin (H. sabdariffa L.) dried calyces beverage on some clinical, biochemical and hematological parameters in humans. Methods: The dried calyces were harvested in the two regions (Adamaoua and West) of Cameroon. The proximate, mineral composition and phytochemical screening were evaluated. A standardized extraction procedure was set up and from the calyces;we prepared a drink for 32 male volunteers’ subjects aged from 21 to 32 years, specially recruited for the experiment. Each participant consumed 500 mL twice a day (in the morning and in the evening) as supplement beverage during two weeks. The anthropometrics (age, height, weight, body mass index (BMI)), clinical (systolic and diastolic blood pressure), hematological (RBC, Hb, PCV, MCV, MCH, MCHC, WBC, Lymphocytes, MID cells, Granulocytes, platelet and MPV) and biochemical (TC, HDL-C, LDL-C, TG, serum iron, blood glucose, creatinine, urea, ASAT and ALAT) parameters were determined in the blood on days 0 and at the end of each week. Results: Crude protein, lipid, fiber and ash content of calyx ranged respectively from 4.57 - 5.98, 10.10 - 11.33, 20.39 - 22.30 and 9.15% - 10.38% while the levels of minerals were ranged from 512.0 - 740.6, 77.8 - 177.7, 52.84 - 52.85, 1.10 - 2.10, 41.2 - 119.5, 3.25 - 8.20 and 0.56 - 17.5 mg/100g respectively for Ca, Mg, K, Na, P, Fe and Zn. The phytochemical screening tests revealed the presence of alkaloids, flavonoids, tannins, phenols and anthocyanins on methanol and aqueous extracts. A significant increase of RBC, Hb, PCV, MPV, HDL-C, TG and creatinine and a significant decrease of WBC, MID cells, LDL-C and TC (p < 0.05) were observed during the study period. Furthermore, there was no significant change on BMI, MCV, MCH, MCHC, lymphocyte, granulocyte, platelet, serum iron, blood glucose, ASAT, ALAT and urea levels. Conclusion: H. sabdariffa L. dried calyces from Cameroon are rich sources of crude fibers and minerals. The H. Sabdariffa L. dried calyces drink can be safely used for people suffering for anemia. It also revealed good cholesterol lowering potential. No hepatoxicity and no kidney damage have been observed as far as serum enzymes were concerned.

Potential use of a dried saliva spot(DSS)in therapeutic drug monitoring and disease diagnosis

In recent years,scientific researchers have increasingly become interested in noninvasive sampling methods for therapeutic drug monitoring and disease diagnosis.As a result,dried saliva spot(DSS),which is a sampling technique for collecting dried saliva samples,has been widely used as an alternative matrix to serum for the detection of target molecules.Coupling the DSS method with a highly sensitive detection instrument improves the efficiency of the preparation and analysis of biological samples.Furthermore,dried blood spots,dried plasma spots,and dried matrix spots,which are similar to those of the DSS method,are discussed.Compared with alternative biological fluids used in dried spot methods,including serum,tears,urine,and plasma,saliva has the advantage of convenience in terms of sample collection from children or persons with disabilities.This review aims to provide integral strategies and guidelines for dried spot methods to analyze biological samples by illustrating several dried spot methods.Herein,we summarize recent advancements in DSS methods from June 2014 to March 2021 and discuss the advantages and disadvantages of the key aspects of this method,including sample preparation and method validation.Finally,we outline the challenges and prospects of such methods in practical applications.

Active tracking of rejected dried blood samples in a large program in Nigeria

AIM: To study the impact of rejection at different levels of health care by retrospectively reviewing records of dried blood spot samples received at the molecular laboratory for human immunodeficiency virus(HIV) early infant diagnosis(EID) between January 2008 and December 2012.METHODS: The specimen rejection rate, reasons for rejection and the impact of rejection at different levels of health care was examined. The extracted data were cleaned and checked for consistency and then deduplicated using the unique patient and clinic identifiers. The cleaned data were ciphered and exported to SPSS version 19(SPSS 2010 IBM Corp, New York, United States) for statistical analyses.RESULTS: Sample rejection rate of 2.4%(n = 786/32552) and repeat rate of 8.8%(n = 69/786) were established. The mean age of infants presenting for first HIV molecular test among accepted valid samples was 17.83 wk(95%CI: 17.65-18.01) vs 20.30 wk(95%CI: 16.53-24.06) for repeated samples. HIV infection rate was 9.8% vs 15.9% for accepted and repeated samples. Compared to tertiary healthcare clinics, secondary and primary clinics had twofold and three-fold higher likelihood of sample rejection, respectively(P < 0.05). We observed a significant increase in sample rejection rate with increasing number of EID clinics(r = 0.893, P = 0.041). The major reasons for rejection were improper sample collection(26.3%), improper labeling(16.4%) and insufficient blood(14.8%). CONCLUSION: Programs should monitor pre-analytical variables and incorporate continuous quality improvement interventions to reduce errors associated with sample rejection and improve patient retention.

沙库巴曲缬沙坦联合冻干重组人脑利钠肽治疗心力衰竭的疗效及对NT-proBNP的影响

目的探讨沙库巴曲缬沙坦联合冻干重组人脑利钠肽治疗心力衰竭的疗效及对NT-proBNP的影响。方法选取2022年4月至2023年5月云南省昆明市第一人民医院心内科收治的150例心力衰竭患者为研究对象,根据就诊时间分为两组。参照组采用沙库巴曲缬沙坦治疗,实验组在参照组基础上使用冻干重组人脑利钠肽治疗。对比两组治疗效果、心功能、血浆BNP、NT-proBNP水平及不良反应发生情况。结果实验组治疗总有效率高于参照组,差异有统计学意义(P<0.05)。实验组干预后心率低于参照组,每搏量及LVEF均高于参照组,差异有统计学意义(P<0.05)。实验组干预后血浆BNP、NT-proBNP水平均低于参照组,差异有统计学意义(P<0.05)。实验组不良反应发生率低于参照组,差异有统计学意义(P<0.05)。结论沙库巴曲缬沙坦联合冻干重组人脑利钠肽治疗心力衰竭可显著提高疗效,降低血浆BNP及NT-proBNP水平,改善患者心功能,减少不良反应。

紫河车的活性成分、药理作用及临床应用研究进展

紫河车是一种名贵的中药药材,中医学很早就有其作用的记载,并将其应用于临床实践中。紫河车的生物活性成分丰富,含有激素、细胞因子、氨基酸、酶及酶抑制因子、微量元素、维生素等,现代药理学研究证明,其有调节免疫、补血、抗肿瘤、抗溃疡、强心、提高抗耐氧能力、减轻疲劳、改善睡眠、延缓衰老、促进子宫、阴道、乳腺、睾丸发育等药理作用,且无明显毒副作用,在临床应用较为广泛,常被应用于不孕不育、先兆流产、更年期综合征、慢性阻塞性肺病、肝炎、肝硬化、难治性溃疡、白癜风、肿瘤等疾病的治疗中,均收到了较为满意的治疗效果。综述有关紫河车生物活性成分、药理作用及临床应用的研究进展,为紫河车在医药及保健等方面的应用提供理论依据,为进一步研究紫河车的药理作用及临床应用提供参考依据。

软组织扩张器联合rhEGF治疗颅骨高压电烧伤的研究

目的探讨颅骨高压电烧伤的治疗方法。方法回顾性收集31例颅骨高压电烧伤患者临床资料,根据治疗方法的不同分为治疗组(16例)和对照组(15例)。治疗组:给予备头皮、清创+rhEGF溶液纱布头皮湿敷+软组织扩张器治疗;对照组:给予单纯创面清创+扩张器治疗。观察两组皮肤扩张时间、皮肤扩张率和即时回缩率等。结果治疗组扩张完成时间、即时回缩率低于对照组(t=6.41,7.69;P<0.01),皮肤扩张率高于对照组(t=23.21,P<0.01)。两组皮瓣全部愈合,效果良好。结论皮肤软组织扩张器联合rhEGF治疗颅骨高压电烧伤效果优良。

冻干重组人脑利钠肽联合呋塞米注射液治疗心力衰竭的效果及对血清PCT、NT-proBNP水平的影响

目的:探讨冻干重组人脑利钠肽联合呋塞米注射液治疗心力衰竭患者的效果及对其血清降钙素原(PCT)、N末端脑钠肽前体(NT-proBNP)水平的影响。方法:选取2019年1月—2022年12月于泰州市第四人民医院接受救治的心力衰竭患者86例为研究对象,根据患者入院日期进行编号,其中43例奇数日期入院的患者纳入对照组,接受呋塞米注射液治疗,另外43例偶数日期入院的患者纳入试验组,接受冻干重组人脑利钠肽联合呋塞米注射液治疗。比较两组临床治疗总有效率、心功能指标[左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)及左心室射血分数(LVEF)]、血液指标(PCT、NT-pro BNP)、生活质量。结果:试验组总有效率为95.35%,明显高于对照组的81.40%,差异有统计学意义(P<0.05)。治疗前,两组LVEDD、LVESD及LVEF水平比较,差异无统计学意义(P>0.05);治疗后,两组LVEDD、LVESD水平均较治疗前降低,LVEF水平较治疗前升高,且试验组LVEDD、LVESD水平低于对照组,LVEF水平高于对照组,差异有统计学意义(P<0.05)。治疗前,两组PCT、NT-proBNP水平比较,差异无统计学意义(P>0.05);治疗后,两组PCT、NT-proBNP水平均较治疗前降低,试验组均低于对照组,差异有统计学意义(P<0.05)。治疗前两组生理功能、健康状况、情感职能、社会功能、躯体疼痛、生理职能、精神健康、精力评分比较,差异无统计学意义(P>0.05);治疗后,两组上述各维度评分均较治疗前升高,且试验组均高于对照组,差异有统计学意义(P<0.05)。结论:冻干重组人脑利钠肽与呋塞米注射液联合治疗心力衰竭患者,其临床治疗总有效率明显提高,心功能明显提升,PCT、NT-proBNP指标水平明显改善,生活质量提高。

冻干重组人脑利钠肽联合呋塞米对难治性心力衰竭患者血流动力学、心肌损伤及肾功能的影响

目的:探讨难治性心力衰竭(RHF)患者采用冻干重组人脑利钠肽联合呋塞米治疗,对其血流动力学、心肌损伤及肾功能的影响。方法:选择200例RHF患者为研究对象,按照治疗方案不同分为对照组(n=113)和观察组(n=87)。两组均给予基础对症治疗,对照组加用呋塞米治疗;观察组在呋塞米治疗的基础上联合冻干重组人脑利钠肽治疗。比较两组患者治疗两周后的临床疗效、血流动力学参数、心肌损伤指标及肾功能指标。结果:治疗两周后,观察组患者治疗有效率高于对照组(P<0.05);患者平均动脉压、中心静脉压等血流动力学参数,血清氨基末端脑钠肽(NT-proBNP)、心肌肌钙蛋白T(cTnT)等心肌损伤指标水平均下降,且观察组低于对照组(P<0.05);两组患者尿素氮(BUN)、血清肌酐(Scr)均降低,但两组间差异无统计学意义(P>0.05);肾小球滤过率(GFR)升高,且观察组高于对照组(P<0.05)。结论:冻干重组人脑利钠肽联合呋塞米有利于提升RHF治疗效果,改善血流动力学指标,缓解心肌损伤,保护肾功能。

甘草干姜汤对人肾癌细胞及人尿源性干细胞的作用

目的:探讨甘草干姜汤(LDGD)对体外培养的人肾癌769-P细胞和人尿源性干细胞(hUSCs)活力和凋亡的作用。方法:常温离心法从人的新鲜尿液中分离培养hUSCs,观察hUSCs的生长状态并采用MTT法测定其活力;流式细胞术鉴定hUSCs表面标志物的表达。MTT法检测2.5~40 mg/mL LDGD作用769-P细胞和hUSCs 24~72 h后的活力,观察其细胞形态的变化;流式细胞术检测10 mg/mL LDGD诱导769-P细胞和hUSCs凋亡的情况,计算其凋亡率。结果:通过常温离心法获得细胞形态良好、生长活性较高的hUSCs,hUSCs表达间充质干细胞表面标志物CD44和CD90,不表达内皮细胞表面标志物CD31和造血干细胞表面标志物CD34。5~10 mg/mL LDGD作用24~72 h可抑制769-P细胞活力并呈时间和剂量-效应关系,而对hUSCs活力有促进作用。10 mg/mL LDGD作用48 h后,倒置显微镜下可见769-P细胞发生明显皱缩,体积缩小,细胞折光率下降,而hUSCs形态无明显变化。10 mg/mL LDGD作用769-P细胞48 h后的凋亡率为(11.93±0.51)%,相较对照组显著升高(P<0.01),而作用hUSCs后的凋亡率为(0.01±0.10)%,与对照组比较差异无统计学意义(P<0.05)。结论:在本实验条件下,LDGD可抑制体外培养的人肾癌细胞活力,诱导其凋亡;可促进hUSCs的细胞活力,且无明显诱导细胞凋亡作用。LDGD对两种细胞的作用存在明显差异。

探讨冻干静注人免疫球蛋白联合注射用头孢哌酮钠舒巴坦钠治疗新生儿感染性肺炎疾病的效果

目的分析新生儿感染性肺炎行冻干静注人免疫球蛋白+头孢哌酮钠舒巴坦钠治疗的效果。方法非随机选取2022年8月—2023年8月巨野县人民医院新生儿科48例感染性肺炎新生儿作为研究对象,按治疗方式不同分为参考组、联合组,各24例,参考组行头孢哌酮钠舒巴坦钠治疗,联合组联用冻干静注人免疫球蛋白治疗。对比两组的观察炎症因子、症状消失时间及疗效。结果联合组的白介素-6、降钙素原以及C反应蛋白均低于参考组,症状消失时间短于参考组,差异有统计学意义(P均<0.05)。联合组的有效率为95.83%(23/24),高于参考组的75.00%(18/24),差异有统计学意义(χ^(2)=4.181,P<0.05)。结论人免疫球蛋白+头孢哌酮钠舒巴坦钠应用于感染性肺炎新生儿能缩短症状消除时间,减轻肺内炎症,有效率高。

冻干重组人脑利钠肽与沙库巴曲缬沙坦钠结合对急性心衰伴肾功能不全的疗效研究

目的探究急性心力衰竭心衰,伴肾功能不全患者实施冻干重组人脑利钠肽与沙库巴曲缬沙坦钠结合治疗的效果。方法随机选取2021年4月-2023年4月淄博市市立医院就诊的70例急性心衰伴肾功能不全患者为研究对象,采用随机数表法将其分为两组,每组35例。两组患者均实施常规治疗,对照组加入沙库巴曲缬沙坦钠治疗,观察组在对照组基础上加以冻干重组人脑利钠肽治疗。比较两组治疗后的肾功能与心功能状况,并观察两组治疗期间存在的不良反应发生情况。结果治疗后,观察组尿素氮、肌酐、B型钠尿肽指标均显著低于对照组,差异有统计学意义(P均<0.05);观察组左室射血分数、血流流速比值水平高于对照组,左心室重塑指数与左心房容积指数水平低于对照组,差异有统计学意义(P均<0.05);观察组的不良反应发生率为11.43%,低于对照组的34.29%,差异有统计学意义(χ^(2)=5.185,P<0.05)。结论对急性心衰伴肾功能不全患者实施冻干重组人脑利钠肽与沙库巴曲缬沙坦钠结合治疗具有较为积极的临床疗效,能够显著改善患者的肾功能与心功能情况,减少不良反应的发生,对改善患者生活质量与整体预后有积极影响。

Cytoprotective effect of amniotic membrane extracts on human corneal epithelial cells exposed to benzalkonium chloride in vitro

Background:The goal was to explore the protective effect and potential mechanism of amniotic membrane extracts(AME)on the ocular surface exposed to benzalkonium chloride(BAC).Methods:The human corneal epithelial cell(HCEC)line SD-HCEC1s was cultured in 5 groups:normal control(NC),NC+AME,BAC,BAC+NC,and BAC+AME.Cell viability analysis,flow cytometry analysis,real-time polymerase chain reaction(PCR),and western blot were employed to measure changes in cell function.Matrix metalloproteinases(MMPs)and inflammatory cytokines were assayed by enzyme-linked immunosorbent assay(ELISA)and activity assays.Results:Real-time PCR and western blot analysis demonstrated that the expressional level of caspase-8 was increased while the levels of Muc1,Muc4,and Muc16 were decreased after treatment with 0.02%BAC for 1 h.When the SD-HCEC1s were withdrawn from the BAC and switched to media containing 10%AME for 2 days,the expression level of capsase-8 was decreased while the levels of Muc1,Muc4,and Muc16 were increased.Real-time PCR and ELISA demonstrated that the mRNA and protein levels of MMP-1,MMP-3,MMP-13,CXCL1,interleukin(IL)-1β,IL-6,and tumor necrosis factor-alpha(TNF-α)were significantly increased after treatment with 0.02%BAC,whereas those of MMP-8 were decreased.When the 0.02%BAC was withdrawn and the SD-HCEC1s were cultured in 10%AME,the mRNA and protein levels of MMP-1,MMP-3,MMP-13,CXCL1,IL-1β,IL-6,and TNF-αwere decreased,while those of MMP-8 were increased.MMP-8 activity assays confirmed that IL-1βand TNF-αdownregulated the protein levels of MMP-8.Conclusions:AME protects SD-HCEC1s when stressed in BAC via upregulation of MMP-8 and downregulation of IL-1βand TNF-α.AME may have the potential functions to be employed as a topical adjunctive therapy in eyes chronically exposed to BAC.

Extraction of Phenolic Compounds from Olive Leaf Extracts and Their Effect on Proliferation of Human Carcinoma Cell Lines

The aim of this work was to evaluate the effect of different olive leaf extracts (OLE) from different leaf growing stages on human carcinoma cell lines. OLE were tested in human carcinoma cell lines in vitro and cells were plated in 96-microtiter culture plates for each OLE concentration. Fresh (F) and freeze-dried (FD) leaves exhibited phenolic compounds in the range of 2.09 ± 0.10 to 8.44 ± 0.64 and 7.72 ± 0.56 to 24.65 ± 1.9 mg gallic acid equivalents/g leaves, respectively. OLE from several Portuguese olive tree cultivars were found to inhibit the growth of human carcinoma cell lines in a range of 2.09 - 8.44 μg phenolic compound/well (209 - 844 μg/ml) and 0.07 - 2.40 μg phenolic compounds/well (7 - 240 μg/ml) for fresh and freeze-dried leaves, respectively. Young (Y) leaves have revealed the highest cell growth inhibition ranging from about 95% for Cobran?osa, followed by 90% for Cobran?osa, 90% for Arbequina and 75% for Arbequina for cell lines A549, HeLa, A431 and OE21, respectively. The lowest cell growth inhibition (35%) was observed for Galega (Y) leaf extract on cell line A549. However, FD samples exhibited a distinctive pattern since cell growth inhibition was highest at highest extract dilution tested, for A431 (Galega Y) followed by A549 (Cobran?osa Y) with cell inhibition of 75% and 70%, respectively. The data presented in this work strongly suggest that OLEs inhibit the growth of human carcinoma cell lines.

腹膜透析结合冻干重组人脑利钠肽治疗尿毒症合并急性左心力衰竭疗效及对患者肾功能影响

目的探讨腹膜透析结合冻干重组人脑利钠肽治疗尿毒症合并急性左心力衰竭(简称左心衰)疗效及对患者肾功能影响。方法选取自2019年3月至2022年12月收治的81例尿毒症合并急性左心衰患者为研究对象,以随机数字表法将患者分为常规组(n=40)和观察组(n=41)。常规组患者实施腹膜透析疗法及硝普钠治疗,观察组患者采取腹膜透析治疗的同时结合冻干重组人脑利钠肽治疗。比较治疗前、后患者血肌酐(Scr)及尿素氮(BUN)等肾功能指标变化、日均尿量以及内生肌酐清除率变化、左心室射血分数(LVEF)以及氨基末端脑肽钠(NT-proBNP)水平变化、白细胞介素6(IL-6)及超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)等炎症因子水平变化。结果观察组的治疗总有效率为95.1%(39/41),显著高于常规组的80.0%(32/40),差异有统计学意义(P<0.05)。治疗前,两组Scr、BUN、日均尿量、内生肌酐清除率、LVEF、NT-proBNP、IL-6、hs-CRP及TNF-α指标比较,差异无统计学意义(P>0.05)。治疗后,两组Scr、BUN、日均尿量、内生肌酐清除率、NT-proBNP、IL-6、hs-CRP及TNF-α均下降,LVEF升高,观察组变化更显著,差异有统计学意义(P<0.05)。结论腹膜透析结合冻干重组人脑利钠肽治疗尿毒症合并急性左心衰疗效显著,患者炎症因子水平显著下降,肾功能改善。

冻干重组人脑利钠肽续贯沙库巴曲缬沙坦钠片在心力衰竭患者治疗中的效果分析

目的探究冻干重组人脑利钠肽续贯沙库巴曲缬沙坦钠片在心力衰竭患者治疗中的效果分析。方法选择2022年4月—2023年4月南京市溧水区人民医院收治的80例心力衰竭患者为研究对象,依据不同干预的方式细分为观察组(冻干重组人脑利钠肽续贯沙库巴曲缬沙坦钠片治疗)与对照组(沙库巴曲缬沙坦钠片单独治疗),各40例。比较心功能、生存质量评分、血浆生化指标、治疗效果、不良反应。结果观察组左心室射血分数、左心室舒张末期内径较对照组更高,左心室收缩末期内径、左心房内径较对照组更低,差异有统计学意义(P<0.05)。观察组社会、环境、躯体、心理评分较对照组更高,差异有统计学意义(P<0.05)。观察组总疗效率(95.00%)高于对照组(70.00%),差异有统计学意义(χ^(2)=7.275,P<0.05)。观察组不良反应1例(2.50%)低于对照组9例(22.50%),差异有统计学意义(χ^(2)=5.304,P<0.05)。治疗后,观察组去甲肾上腺素、血浆醛固酮、N末端B型钠尿肽前体水平低于对照组,差异有统计学意义(P<0.05)。结论用冻干重组人脑利钠肽续贯沙库巴曲缬沙坦钠片治疗心力衰竭患者,将明显改善患者心功能,提升生存质量,降低不良反应发生率,提升对心力衰竭的治疗效果,改善血浆生化指标。

冻干重组人脑利钠肽联合螺内酯治疗老年高血压合并急性心力衰竭的临床研究

目的探讨冻干重组人脑利钠肽联合螺内酯治疗老年高血压合并急性心力衰竭(AHF)的临床疗效。方法选取2019年3月至2021年3月上饶市立医院确诊的75例老年高血压合并AHF患者作为研究对象,按照随机数字表法分组为观察组(n=37)与对照组(n=38)。对照组采用冻干重组人脑利钠肽治疗,观察组采用冻干重组人脑利钠肽联合螺内酯治疗,比较两组临床疗效、治疗前后炎症因子[N端脑钠肽原(NT-proBNP)、血清肌钙蛋白T(TnT)]、血压[收缩压(SBP)、舒张压(DBP)]、心功能指标[左心室射血分数(LVEF)、左心室舒张末期容积(LVEDV)、左心室收缩末期内径(LVESD)]及不良反应发生情况。结果观察组治疗总有效率为86.48%,对照组为81.58%,两组比较差异无统计学意义。治疗后,观察组NT-proBNP、TnT水平均低于对照组,差异有统计学意义(P<0.05)。治疗后,观察组SBP、DBP水平均低于对照组,差异有统计学意义(P<0.05)。治疗后,观察组LVEF高于对照组,LVEDV和LVESD均小于对照组,差异有统计学意义(P<0.05)。观察组不良反应发生率为10.81%,对照组为15.79%,两组比较差异无统计学意义。结论冻干重组人脑利钠肽联合螺内酯治疗老年高血压合并AHF,可明显降低患者炎症因子及血压水平,改善患者心功能,且安全性较高,值得临床推广应用。

冻干重组人脑利钠肽治疗慢性心力衰竭合并肺动脉高压的效果及不良反应发生率分析

目的探究冻干重组人脑利钠肽(rhBNP)治疗慢性心力衰竭(CHF)合并肺动脉高压(PH)的效果及对不良反应发生率的影响。方法选取2020年1月至2021年5月邹城市人民医院千泉院区收治的70例CHF合并PH患者作为研究对象,随机分为观察组与对照组,每组35例。对照组行常规治疗,观察组在对照组基础上联合rhBNP治疗,比较两组心功能指标[左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)]、N端脑钠肽前体(NT-proBNP)、24 h尿量、心率(HR)、肺动脉收缩压(PASP)、炎症因子指标[白细胞介素-10(IL-10)、C反应蛋白(CRP)、白细胞介素-6(IL-6)]、体征及症状消失时间、不良反应发生率及临床疗效。结果治疗后,两组LVEDD、LVESD均短于治疗前,LVEF均高于治疗前,且观察组LVEDD、LVESD均短于对照组,LVEF高于对照组,差异有统计学意义(P<0.05)。治疗后,两组NT-proBNP水平、PASP均低于治疗前,24 h尿量均多于治疗前,HR均慢于治疗前,且观察组NT-proBNP水平、PASP均低于对照组,24 h尿量多于对照组,HR慢于对照组,差异有统计学意义(P<0.05)。治疗后,两组IL-10水平均高于治疗前,CRP、IL-6水平均低于治疗前,且观察组IL-10水平高于对照组,CRP、IL-6水平均低于对照组,差异有统计学意义(P<0.05)。观察组发绀、啰音、咳嗽、水肿消失时间均短于对照组,差异有统计学意义(P<0.05)。两组不良反应发生率比较差异无统计学意义。观察组治疗总有效率为97.14%,高于对照组的77.14%,差异有统计学意义(P<0.05)。结论rhBNP治疗CHF合并PH患者疗效确切,可改善患者心功能,更好地控制HR,增加尿量,降低NT-proBNP水平及PH,加快体征及症状消失时间,且安全性高,值得临床推广应用。