Physiological Response Mechanism and Drought Resistance Evaluation of Passiflora edulis Sims under Drought Stress

In order to explore the response mechanism of Passiflora edulis Sims to drought stress,the changes in morpho-logical and physiological traits of Passiflora edulis Sims under different drought conditions were studied.A total of 7 germplasm resources of Passiflora edulis Sims were selected and tested under drought stress by the pot culture method under 4 treatment levels:75%–80%(Control,CK)of maximumfield water capacity,55%–60%(Light Drought,LD)of maximumfield water capacity,i.e.,mild drought,40%–45%(Moderate Drought,MD)of max-imumfield water capacity,i.e.,moderate drought and 30%–35%(Severe Drought,SD)of maximumfield water capacity,i.e.,severe drought.On the 40th day of drought treatment,13 indices,including seedling growth mor-phology,physiology,and biochemistry,were measured.The results showed that under drought stress,the height and ground diameter of P.edulis Sims gradually decreased with increasing drought stress,and there were signifi-cant differences in seedling height and ground diameter among the treatments.Drought stress significantly inhib-ited the growth of seven P.edulis Sims varieties.The contents of soluble sugar(SS),soluble protein(SP),proline(Pro),and other substances in P.edulis Sims basically increased with increasing drought stress.With the aggrava-tion of drought stress,the malondialdehyde(MDA)content of P.edulis Sims tended to increase to different degrees,the superoxide dismutase(SOD)activity and peroxidase(POD)activity both tended to increase atfirst and then decrease,and the change in catalase(CAT)activity mostly showed a gradual increasing trend.The con-tents of endogenous hormones in P.edulis Sims significantly differed under different degrees of drought stress.With the aggravation of drought stress,the abscisic acid(ABA)content of P.edulis Sims tended to increase,whereas the contents of gibberellin(GA),indoleacetic acid(IAA),and zeatin nucleoside(ZR)exhibited a down-ward trend.A comprehensive evaluation of the drought resistance of seven P.edulis Sims varieties was conducted based on the principal component analysis method,and the results showed that the drought resistance decreased in the order XH-BL>XH-TWZ>TN1>GH1>ZJ-MT>LP-LZ>DH-JW.

Physiological and Transcriptome Analysis Illuminates the Molecular Mechanisms of the Drought Resistance Improved by Alginate Oligosaccharides in Triticum aestivum L.

Alginate oligosaccharides(AOS)enhance drought resistance in wheat(Triticum aestivum L.),but the definite mechanisms remain largely unknown.The physiological and transcriptome responses of wheat seedlings treated with AOS were analyzed under drought stress simulated with polyethylene glycol-6000.The results showed that AOS promoted the growth of wheat seedlings and reduced oxidative damage by improving peroxidase and superoxide dismutase activities under drought stress.A total of 10,064 and 15,208 differentially expressed unigenes(DEGs)obtained from the AOS treatment and control samples at 24 and 72 h after dehydration,respectively,were mainly enriched in the biosynthesis of secondary metabolites(phenylpropanoid biosynthesis,flavonoid biosynthesis),carbohydrate metabolism(starch and sucrose metabolism,carbon fixation in photosynthetic organisms),lipid metabolism(fatty acid elongation,biosynthesis of unsaturated fatty acids,alpha-linolenic acid metabolism,cutin,suberine and wax biosynthesis),and signaling transduction pathways.The up-regulated genes were related to,for example,chlorophyll a-b binding protein,amylosynthease,phosphotransferase,peroxidase,phenylalanine ammonia lyase,flavone synthase,glutathione synthetase.Signaling molecules(including MAPK,plant hormones,H_(2)O_(2) and calcium)and transcription factors(mainly including NAC,MYB,MYB-related,WRKY,bZIP family members)were involved in the AOS-induced wheat drought resistance.The results obtained in this study help underpin the mechanisms of wheat drought resistance improved by AOS,and provides a theoretical basis for the application of AOS as an environmentally sustainable biological method to improve drought resistance in agriculture.

Ghrelin regulates insulin resistance by targeting insulin-like growth factor-1 receptor via miR-455-5p in hepatic cells

Objective: To explore the mechanism by which ghrelin regulates insulin sensitivity through modulation of miR-455-5p in hepatic cells. Methods: HepG2 cells were treated with or without DAG (1 μM). Glucose consumption, intracellular glycogen content, phosphorylation of PI3K and Akt stimulated by insulin, expression of miR-455-5p, as well as IGF-1R protein level were analyzed. In addition, bioinformatic analysis, dual luciferase reporter assay, miR- 455-5p mimic or inhibitor treatment was conducted to investigate the molecular mechanisms. Results: High glucose treatment upregulated miR-455-5p expression but reduced glucose consumption and glycogen content. DAG reversed the effect of high glucose on glucose metabolism, increased protein level of IGF-1R and phosphorylation of PI3K/Akt stimulated by insulin, as well as downregulated miR-455-5p expression. Bioinformatic analysis indicated IGF-1R was the target of miR-455-5p. Dual luciferase reporter assay, as well as transfection with miR-455-5p mimic/inhibitor confirmed that DAG activated IGF-1R/PI3K/Akt signaling via inhibiting miR-455-5p. Conclusion: DAG improves insulin resistance via miR-455-5p- mediated activation of IGF-1R/PI3K/Akt system, suggesting that suppression of miR-455-5p or activation of DAG may be potential targets for T2DM therapy.

PoWRKY71 is involved in Paeonia ostii resistance to drought stress by directly regulating light-harvesting chlorophyll a/b-binding 151 gene

Although the functions of WRKY transcription factors in drought resistance are well known,their regulatory mechanisms in response to drought by stabilising photosynthesis remain unclear.Here,a differentially expressed PoWRKY71 gene that was highly expressed in drought-treated Paeonia ostii leaves was identified through transcriptome analysis.PoWRKY71 positively responded to drought stress with significantly enhanced expression patterns and overexpressing PoWRKY71 in tobacco greatly improved plant tolerance to drought stress,whereas silencing PoWRKY71 in P.ostii resulted in a drought-intolerant phenotype.Furthermore,lower chlorophyll contents,photosynthesis,and inhibited expression of photosynthesis-related light-harvesting chlorophyll a/b-binding 151(CAB151)gene were found in PoWRKY71-silenced P.ostii.Meanwhile,a homologous system indicated that drought treatment increased PoCAB151 promoter activity.Interactive assays revealed that PoWRKY71 directly bound on the W-box element of PoCAB151 promoter and activated its transcription.In addition,PoCAB151 overexpressing plants demonstrated increased drought tolerance,together with significantly higher chlorophyll contents and photosynthesis,whereas these indices were dramatically lower in PoCAB151-silenced P.ostii.The above results indicated that PoWRKY71 activated the expression of PoCAB151,thus stabilising photosynthesis via regulating chloroplast homeostasis and chlorophyll content in P.ostii under drought stress.This study reveals a novel drought-resistancemechanism in plants and provides a feasible strategy for improving plant drought resistance via stabilising photosynthesis.

Grassland-type ecosystem stability in China differs under the influence of drought and wet events

Ecological stability is a core issue in ecological research and holds significant implications forhumanity. The increased frequency and intensity of drought and wet climate events resulting from climatechange pose a major threat to global ecological stability. Variations in stability among different ecosystemshave been confirmed, but it remains unclear whether there are differences in stability within the sameterrestrial vegetation ecosystem under the influence of climate events in different directions and intensities.China's grassland ecosystem includes most grassland types and is a good choice for studying this issue.This study used the Standardized Precipitation Evapotranspiration Index-12 (SPEI-12) to identify thedirections and intensities of different types of climate events, and based on Normalized DifferenceVegetation Index (NDVI), calculated the resistance and resilience of different grassland types for 30consecutive years from 1990 to 2019 (resistance and resilience are important indicators to measurestability). Based on a traditional regression model, standardized methods were integrated to analyze theimpacts of the intensity and duration of drought and wet events on vegetation stability. The resultsshowed that meadow steppe exhibited the highest stability, while alpine steppe and desert steppe had thelowest overall stability. The stability of typical steppe, alpine meadow, temperate meadow was at anintermediate level. Regarding the impact of the duration and intensity of climate events on vegetationecosystem stability for the same grassland type, the resilience of desert steppe during drought was mainlyaffected by the duration. In contrast, the impact of intensity was not significant. However, alpine steppewas mainly affected by intensity in wet environments, and duration had no significant impact. Ourconclusions can provide decision support for the future grassland ecosystem governance.

Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

Increase in root density induced by coronatine improves maize drought resistance in North China

Drought stress caused by insufficient irrigation or precipitation impairs agricultural production worldwide.In this study,a two-year field experiment was conducted to investigate the effect of coronatine(COR),a functional analog of jasmonic acid(JA),on maize drought resistance.The experiment included two water treatments(rainfed and irrigation),four COR concentrations(mock,0μmol L^(-1);A1,0.1μmol L^(-1);A2,1μmol L^(-1);A3,10μmol L^(-1))and two maize genotypes(Fumin 985(FM985),a drought-resistant cultivar and Xianyu 335(XY335),a drought-sensitive cultivar).Spraying 1μmol L^(-1)COR at seedling stage increased surface root density and size,including root dry matter by 12.6%,projected root area by 19.0%,average root density by 51.9%,and thus root bleeding sap by 28.2%under drought conditions.COR application also increased leaf area and SPAD values,a result attributed to improvement of the root system and increases in abscisic acid(ABA),JA,and salicylic acid(SA)contents.The improvement of leaves and roots laid the foundation for increasing plant height and dry matter accumulation.COR application reduced anthesis and silking interval,increasing kernel number per ear.COR treatment at 1μmol L^(-1)increased the yield of XY335 and FM985 by 7.9%and 11.0%,respectively.Correlation and path analysis showed that grain yields were correlated with root dry weight and projected root area,increasing maize drought resistance mainly via leaf area index and dry matter accumulation.Overall,COR increased maize drought resistance mainly by increasing root dry weight and root area,with 1μmol L-^(-1)COR as an optimal concentration.

Genetic dissection of drought resistance for trait improvement in crops

Reliance on agriculture for food security is a constant in all modern societies.Global climate change and population growth have put immense pressure on sustainable agriculture,exacerbating the effects of environmental stresses.Drought is one of the most pressing abiotic stresses that farmers face,presenting an annual threat to crop growth and yield.Crops have evolved extensive morphological,physiological,and molecular mechanisms to combat drought stress.Drought resistance is a polygenic trait,controlled by a complex genetic network and an array of genes working together to ensure plant survival.Many studies have aimed at dissecting the genetic mechanisms underlying drought resistance.Recent studies using linkage and association mapping have made progress in identifying genetic variations that affect drought-resistance traits.These loci may potentially be engineered by genetic transformation and genome editing aimed at developing new,stress-resistant crop cultivars.Here we summarize recent progress in elucidating the genetic basis of crop drought resistance.Molecular-breeding technologies such as marker-assisted selection,genome selection,gene transformation,and genome editing are currently employed to develop drought-resistant germplasm in a variety of crops.Recent advances in basic research and crop biotechnology covered in this review will facilitate delivery of drought-resistant crops with unprecedented efficiency.

Comprehensive Evaluation on Drought Resistance of Different Flue-cured Tobacco Cultivars in Bijie Tobacco Growing Area

Based on pot experiments, major agronomic traits, biomass accumulation, leaf water-holding capacity, relative water content, root MDA content, root proline content and other physiological indicators of four different flue-cured tobacco culti-vars under drought stress were investigated, and drought resistance in various flue-cured tobacco cultivars was comprehensively analyzed with subordinate function method, aiming at clarifying the differences in drought resistance among various flue-cured tobacco cultivars. The results indicated that under drought stress, major agronomic traits, fresh and dry mass accumulation, and leaf relative water content of four different flue-cured tobacco cultivars were reduced significantly; the decreas-ing range of Yunyan 87 reached the minimum, fol owed by Bina 1, while Qianxi 1 demonstrated the maximum decreasing range. Leaf water-holding capacity of various flue-cured tobacco cultivars showed a descending order of Yunyan 87 〉 Bina 1 〉Qianxi 1 〉 Jiucaiping 2. MDA content and proline content in roots of various flue-cured tobacco cultivars increased significantly; to be specific, Yunyan 87 and Bina 1 exhibited a slight increase in root MDA content and a significant increase in root proline content, while Jiucaiping 2 and Qianxi 1 showed an opposite trend. Accord-ing to the results of comprehensive analysis with subordinate function method, drought resistance in various flue-cured tobacco cultivars showed a descending or-der of Yunyan 87 〉 Bina 1 〉 Jiucaiping 2 〉 Qianxi 1.

Identification and Evaluation of Insect and Disease Resistance in Transgenic Cry1Ab13-1 and NPR1 Maize

PCR detection,quantitative real-time PCR(q-RTPCR),outdoor insect resistance,and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations(T2,T3,and T4)in transgenic maize germplasms(S3002 and 349)containing the bivalent genes(insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1)and their corresponding wild type.Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations;q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots,stems,and leaves of tested maize plants.In addition,S3002 and 349 bivalent gene-transformed lines exhibited resistance to large leaf spots and corn borer in the field evaluation compared to the wild type.Our study confirmed that Cry1Ab13-1 and NPR1 bivalent genes enhanced the resistance against maize borer and large leaf spot disease and can stably inherit.These findings could be exploited for improving other cultivated maize varieties.

Relationship Between Changes of Endogenous Hormone in Sweet Potato Under Water Stress and Variety Drought-resistance

The IAA, GA3, iPA, ZR and ABA contents in sweet potato leaves under drought conditions were determined by the method of ELISA, and the relationship between these endogenous hormones and drought resistance of different sweet potato varieties were studied. The results showed the IAA, GA3, iPA and ZR contents in sweet potato leaves decreased, but ABA contents increased obviously. The stronger the drought resistance of the variety was, the more IAA, GA3, iPA and ZR contents decreased and the less ABA contents increased. Their relative contents correlated significantly negatively(r were -0.9070, -0.9493, -0.9509, -0.8674 and -0.9117 respectively)to drought-resistability.

The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance

DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance.

Silencing MdGH3-2/12 in apple reduces drought resistance by regulating AM colonization

Drought leads to reductions in plant growth and crop yields.Arbuscular mycorrhizal fungi(AMF),which form symbioses with the roots of the most important crop species,alleviate drought stress in plants.In the present work,we identified 14 GH3 genes in apple(Malus domestica)and provided evidence that MdGH3-2 and MdGH3-12 play important roles during AM symbiosis.The expression of both MdGH3-2 and MdGH3-12 was upregulated during mycorrhization,and the silencing of MdGH3-2/12 had a negative impact on AM colonization.MdGH3-2/12 silencing resulted in the downregulation of five genes involved in strigolactone synthesis,and there was a corresponding change in root strigolactone content.Furthermore,we observed lower root dry weights in RNAi lines under AM inoculation conditions.Mycorrhizal transgenic plants showed greater sensitivity to drought stress than WT,as indicated by their higher relative electrolytic leakage and lower relative water contents,osmotic adjustment ability,ROS scavenging ability,photosynthetic capacity,chlorophyll fluorescence values,and abscisic acid contents.Taken together,these data demonstrate that MdGH3-2/12 plays an important role in AM symbiosis and drought stress tolerance in apple.

Relationship Between Serum microRNA-372-3p and Glucose Transporter 4 Levels and Insulin Resistance in Gestational Diabetes Mellitus

Objective:To observe the changes in insulin resistance in patients with gestational diabetes mellitus(GDM)based on the detection of serum microRNA-372-3p and glucose transporter protein 4(GLUT4)levels.Methods:We conducted a retrospective cohort study of 42 patients who were diagnosed with GDM and hospitalized in our hospital during the period from January 2017 to December 2021 and another 42 patients who had normal pregnancy during the same period by collecting their clinical data.We analyzed their serum microRNA expression profiles and miR-372-3p levels to study the relationship between GDM and insulin resistance.Results:The relative expression of miR-372-3p in the serum of patients in the GDM group was significantly higher than that of patients in the control group,but the GLUT 4 level of the GDM group was significantly lower than that of the control group(P<0.05).Compared with the control group,the GDM group had significantly higher levels of fasting blood glucose(FBG),fasting insulin(FINS),2-hour postprandial blood glucose(2h-BG),total cholesterol(TC),triglyceride(TG),and homeostatic model assessment for insulin resistance(HOMA-IR)index but significantly lower homeostasis model assessment ofβ-cell function(HOMA-β)index(P<0.05).The relative expression of miR-372-3p in serum was independently and positively correlated with HOMA-IR,while the level of GLUT4 was independently and negatively correlated with HOMA-IR(P<0.05).Conclusion:Glycosylated hemoglobin test in the early stages of pregnancy(12–13 weeks of gestation)is important to ensure the health of pregnant women and fetuses.The screening and intervention for elevated glucose in pregnant women act as a guideline for the treatment of GDM.Patients with insulin resistance and related complications such as hyperinsulinemia and hypoglycemia should be given priority.

Rapid Identification for Drought Resistance of Wheat Using Near-infrared Diffuse Reflectance Spectroscopy

[Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drought resistance were selected and were classified according to their drought resistance grades determined by the Technical Specification of Identification and Evaluation for Drought Resistance in Wheat （GB/T 21127-2007）. In addition, the harvested wheat seed samples were spectrally analyzed with FOSS NIRSystems5000 near-infrared spectrum analyzer for grain quality （full spectrum analyzer） and then the forecasted regression equations were established. [Result] After the establishment of a database and validation, dis- criminated functions were obtained. The determination coefficient （RSQ） and coeffi- cients of determination for cross validation （1-VR） in the discriminant function built with seed samples from water stress area were 0.846 0 and 0.781 8, respectively, which indicated that the consistency between drought resistance and spectral charac- teristics in wheat varieties was good, and there was high correlation between the near-infrared diffuse reflectance spectra of seeds and the drought resistance in wheat. [Conclusiou] Under water stress condition, it is feasible to establish a conve- nient, rapid and no-damage identification system for the drought resistance in wheat by using the near-infrared diffuse reflectance spectrum technique to scan wheat seeds.

Relationship Between Physiological-biochemical Changes Under Drought Stress and Drought Resistance in Ramie

A study on the physiological-biochemical changes of different ramie(Boehmeria Jacq. ) varieties under drought stress was carried out, the results showed that relative water contents(RWC) decreased and RPP increased with the increase of drought stress. Compared with drought sensitive varieties, drought resistant varieties had higher RWC in leaves but lower RPP. Peroxidase activity of drought-resistance varieties changed from low to high whereas sensitive ones changed in opposite direction. Proline contents of drought sensitive varieties was higher than those of drought resistant ones under a certain degree drought stress. Proline accumulation in drought resistant varieties was faster than that in drought sensitive ones under serious drought stress.MDA contents in leaf was increased under drought stress. MDA contents increased slowly in resistant varieties while that increased rapidly in sensitive ones.

Long-Term No-Tillage Direct Seeding Mode for Water-Saving and Drought-Resistance Rice Production in Rice-Rapeseed Rotation System

To study the effects of long-term no-tillage direct seeding mode on rice yield and the soil physiochemical property in a rice-rapeseed rotation system, a comparative experiment with a water-saving and drought-resistance rice （WDR） variety and a double low rapeseed variety as materials was conducted under no-tillage direct seeding （NTDS） mode and conventional tillage direct seeding （CTDS） mode for four years, using the CTDS mode as the control. Compared with the CTDS mode, the actual rice yield of WDR decreased by 8.10% at the first year, whereas the plant height, spikelet number per panicle, spikelet fertility, 1000-grain weight, grain yield, actual yield, and harvest index increased with no-tillage years, which led to the actual yield increase by 6.49% at the fourth year. Correlation analysis showed that the panicle length was significantly related to the actual yield of WDR. Compared with the CTDS mode in terms of the soil properties, the pH value of the NTDS mode decreased every year, whereas the contents of soil organic matter and total N of the NTDS mode increased. In the 0-5 cm layer of the NTDS mode, the soil bulk decreased, whereas the contents of soil organic matter, total N, and available N increased. In the 5-20 cm layer of the NTDS mode, the available N and K decreased, whereas the soil bulk, contents of soil organic matter, and total N increased. In summary, the NTDS mode increased the rice yield, and could improve the paddy soil fertility of the top layer.

Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

Soybean(Glycine max(L.)Merr.)is an important cultivated crop,which requires much water during its growth,and drought seriously affects soybean yields.Studies have shown that the expression of small heat shock proteins can enhance drought resistance,cold resistance and salt resistance of plants.In this experiment,soybean GmHsps_p23-like gene was successfully cloned by RT-PCR,the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis,and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed.Agrobacterium-mediated method was used to transform soybeans to obtain positive plants.RT-PCR detection,rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T2 generation positive transgenic soybean plants identified by PCR and Southern hybridization.The results showed that the overexpression vector plant GmHsps_p23-like gene expression increased.After rehydration,the transgenic overexpression plants returned to normal growth,and the damage to the plants was low.After drought stress,the SOD and POD activities and the PRO content of the transgenic overexpression plants increased,while the MDA content decreased.The reverse was true for soybean plants with genetically modified editing vectors.The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group,and had a stronger drought resistance.It showed that the expression of soybean GmHsps_p23-like gene can improve the drought resistance of soybean.The cloning and functional verification of soybean GmHsps_p23-like gene had not been reported yet.This is the first time that PCR technology has been used to amplify the soybean GmHsps_p23-like gene and construct an expression vector for this gene.This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties.

Study on Diversity of Soybean Germplasm with Drought Resistance in Huang-Huai-Hai Region

Fifty soybean germplasms with drought resistance selected from Huang-Huai-Hai Region were studied under field drought condition. The results showed that there was a diversity of drought resistance at different growth stages. Some varieties had drought resistance in whole growing period, but some only at one stage or several stages. Some varieties had both drought resistance and higher yield characters, some with drought resistance but lower yield. It was also found in present study that some drought resistant germplasms

Comparison of Drought-Resistance about Three Wild Rocky-Grasses

In this experiment, using the methods of drought-stress in the pot and PEG simulative drought-stress, three native rocky-slope grasses Pogonatherum panideum（Lam.） Hack, Erioophorum comosum nees and Cynodon dactylon cultivated in the pots were selected as materials to study their drought-resistance by analyzing the indexes such as the leaf area index, the drying roots accumulation, the holding water ability of leaf, the relative conductance of leaf, the soluble protein, chlorophyll. At the same time, by comparing with membership function value of each index, the order of each grass drought-resistance was decided. The results showed that the drought-resistance of Erioophorum comosum nees was stronger than Cynodon dactylon and drought-resistance of Cynodon dactylon was stronger than Pogonatherum panideum （Lam.） Hack. The purpose of the experimental results was to find theoretical foundations for selecting and cultivating native protecting-slope vegetations to adapt to rocky slopes.