目的:探讨南阳市第一人民医院抗菌药物的使用情况及与常见的细菌耐药的相关性。方法:收集南阳市第一人民医院2018年1月至2021年12月临床患者送检的阳性标本,统计临床标本分离病原菌检出情况、常见抗菌药物消耗情况以及常见的革兰氏阴性...目的:探讨南阳市第一人民医院抗菌药物的使用情况及与常见的细菌耐药的相关性。方法:收集南阳市第一人民医院2018年1月至2021年12月临床患者送检的阳性标本,统计临床标本分离病原菌检出情况、常见抗菌药物消耗情况以及常见的革兰氏阴性杆菌、阳性球菌对抗菌药物产生的耐药率(%)与抗菌药物用药频度(Frequency of defined daily doses,DDDs)的相关性。结果:我院2018年至2021年抗菌药物DDDs较高的抗菌药物依次为美洛西林、头孢他啶、哌拉西林他唑巴坦,检出率较高的病原菌分别为大肠埃希氏菌、肺炎克雷伯菌、金黄色葡萄球菌等。大肠埃希菌产生的耐药率与哌拉西林他唑巴坦、头孢呋辛钠DDDs均有相关性(r=-0.107、0.432);肺炎克雷伯菌产生的耐药率与哌拉西林他唑巴坦、左氧氟沙星氯化钠、头孢呋辛钠DDDs呈正相关(r=0.410、0.720、0.420);铜绿假单胞菌与哌拉西林他唑巴坦、头孢哌酮舒巴坦钠DDDs呈正相关(r=0.716、0.933);金黄色葡萄球菌的耐药率与左氧氟沙星DDDs呈正相关(r=0.661);肺炎链球菌产生的耐药率与左氧氟沙星、头孢呋辛钠DDDs均存在正相关关系(r=0.416和0.482)。结论:抗菌药物的DDDs与常见细菌产生的耐药性具有一定的相关性,我院应严格控制抗菌药物的DDDs,根据感染性病种及药敏试验结果选择适当的抗菌药,加强常见病原菌的耐药监测。展开更多
The different investigation has been carried out on the biological activities of titanium dioxide nanoparticle but the effect of this nano product on the antibacterial activity of different antibiotics has not been ye...The different investigation has been carried out on the biological activities of titanium dioxide nanoparticle but the effect of this nano product on the antibacterial activity of different antibiotics has not been yet demonstrated. In this study the nano size TiO2 is synthesized using citric acid and alpha dextrose and the enhancement effect of TiO2 nanoparticle on the antibacterial activity of different antibiotics was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA). During the present study, different concentrations of nano-scale TiO2 were tested to find out the best concentration that can have the most effective antibacterial property against the MRSA culture. Disk diffusion method was used to determine the antibacterial activity of these antibiotics in the absence and presence of sub inhibitory concentration of TiO2 nano particle. A clinical isolate of MRSA, isolated from Intensive Care Unit (ICU) was used as test strain. In the presence of sub-inhibitory concentration of TiO2 nanoparticle (20 μg/disc) the antibacterial activities of all antibiotics have been increased against test strain with minimum 2 mm to maximum 10mm. The highest increase in inhibitory zone for MRSA was observed against pencillin G and amikacin (each 10 mm). Conversely, in case of nalidixic acid, TiO2 nanoparticle showed a Synergic effect on the antibacterial activity of this antibiotic against test strain. These results signify that the TiO2 nanoparticle potentate the antimicrobial action of beta lactums, cephalosporins, aminoglycosides, glycopeptides, macrolids and lincosamides, tetracycline a possible utilization of nano compound in combination effect against MRSA.展开更多
Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the stu...Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the study, the exonic and 3’UTR sequences of five genes in 12 sporadic colorectal tumors were extracted by next generation sequencing. In tumors with variation in the 3’UTR region, the changes caused by the variation in the miRNA binding profile were detected. The expression profile of these miRNAs in colorectal and other solid tumors compared to normal tissue was determined. Pathway analysis was performed to determine which signaling pathways miRNAs affect. Results: Case-10 in our study was wild type KRAS and received cetuximab treatment and developed drug resistance. In this case, it was concluded that the expression of KRAS increased and tumorigenesis progressed due to miRNAs that do not bind to this region due to variations in the 3’UTR region. Among these miRNAs, hsa-miR-124-3p was found to have decreased expression in colorectal tumors and to be associated with the ECM-receptor interaction pathway. Conclusion: Variations in the 3’UTR regions of genes critical in the process of carsinogenesis are associated with drug resistance and the process of tumorigenesis.展开更多
Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic...Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic bacteria identification and drug sensitivity tests were performed with a VITEK 2 compact automatic identification system and data were analyzed using WHONET5.6 software.Results： Of the 1,378 strains tested, 980 were Gram-negative bacilli, accounting for 71.1%, in which Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa were the dominant strains. We found 328 Gram-positive coccus, accounting for 23.8%, in which the amount of Staphylococcus aureus was the highest. We identified 46 fungi, accounting for 4.1%. According to the departmental distribution within the hospital, the surgical departments isolated the major strains, accounting for 49.7%. According to disease types, lung cancer, intestinal cancer and esophagus cancer were the top three, accounting for 20.9%, 17.3% and 14.2%, respectively. No strains were resistant to imipenem, ertapenem or vancomycin.Conclusions： Pathogenic bacteria isolated from the specialized cancer hospital have different resistance rates compared to commonly used antimicrobial agents; therefore antimicrobial agents to reduce the morbidity and mortality of infections should be used.展开更多
Objective:To provide evidence for a rational and effective prevention and treatment of Pseudomonas aeruginosa,the clinical characteristics and the resistance to various antibiotics of were investigated.Methods:A retro...Objective:To provide evidence for a rational and effective prevention and treatment of Pseudomonas aeruginosa,the clinical characteristics and the resistance to various antibiotics of were investigated.Methods:A retrospective analysis of 224 strains of Pseudomonas aeruginosa isolated from various specimens from various clinical departments of our hospital(April 1,2018 to June 31,2019)were conducted.Identification and drug susceptibility test of isolated strains was performed using a fully automatic bacterial identification analyzer(MicroScan WalkAway-96 plus),and data analysis was performed using WH0NET5.6 software.Results:Among all the bacteria isolated in our hospital during the above period,Pseudomonas aeruginosa accounted for 10.09% of them all and 12.57% of Gram-negative bacilli,respectively.These isolates were mainly derived from sputum specimens(68.75%),mainly from male patients(70.54%),and mostly 61-70(27.23%)or 51-60(22.77%)years old.Pseudomonas aeruginosa isolates are mainly from Rehabilitation Ward,ICU,and Liver Transplantation Unit,accounted for 29.91%,12.95% and 10.27% of all isolates,respectively.The sensitivity of Pseudomonas aeruginosa to various antibacterial drugs,in the order of high to low were carbapenems,aztreonam,quinolones,cephalosporins,piperacillin/tazobactam,aminoglycoside,with a lowest resistance rate(2.4%)to amikacin and a highest resistance rate to imipenem(33.0%).Conclusion:The isolation rate of Pseudomonas aeruginosa was relatively stable during the study period,and among all the P.aeruginosa detected,most of them were from the respiratory secretions of elderly male patients.The resistance rate of Pseudomonas aeruginosa isolates to various antibiotics is mainly within 30%.Clinical units such as Rehabilitation Ward,ICU,and Liver Transplantation Unit have a high detection rate;therefore,these departments should be monitored in a focused manner.Our research provides a scientific basis for the rational use of antibiotics and a better control of Pseudomonas aeruginosa infection.展开更多
Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatmen...Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatment for the infection of Klebsiella pneumoniae.Methods:1,192 strains of Klebsiella pneumoniae isolated from clinical specimens from 2012 to 2016 were collected.The strains were identified by VITEK-2 Compact Microbiological Identification System,and the corresponding results of the antimicrobial susceptibility test were interpreted in accordance with the standards recommended by Clinical and Laboratory Standards Institute(CLSI).Results:1,192 strains of Klebsiella pneumoniae were mainly isolated from sputum(65.6%),and most of them were from Respiratory Medicine Department and Medical Intensive Care Unit of Respiratory Medicine Department(MICU),accounting for 41.4%.Out of 1,192 strains,448 strains were detected to produce extended-spectrum beta-lactamases(ESBLs),accounting for 37.6%.In addition,the detection rates of ESBL-producing Klebsiella pneumoniae for 5 consecutive years showed an increasing trend year by year,and they were higher than the national average values published by China Antimicrobial Resistance Surveillance System(CARSS)in the corresponding period.The drug resistance rate of ESBL-producing Klebsiella pneumoniae was significantly higher than that of non ESBL-producing strains.Conclusions:The infection caused by Klebsiella pneumoniae mainly occurs in the lower respiratory tract,and the drug resistance rates of Klebsiella pneumoniae to antibiotics in the drug susceptibility spectrum are maintained at a high level.Therefore,the rational selection of antibiotics for the clinical treatment of lower respiratory tract infection caused by Klebsiella pneumoniae must be based on the production of ESBLs and the results of antimicrobial susceptibility test.展开更多
文摘目的:探讨南阳市第一人民医院抗菌药物的使用情况及与常见的细菌耐药的相关性。方法:收集南阳市第一人民医院2018年1月至2021年12月临床患者送检的阳性标本,统计临床标本分离病原菌检出情况、常见抗菌药物消耗情况以及常见的革兰氏阴性杆菌、阳性球菌对抗菌药物产生的耐药率(%)与抗菌药物用药频度(Frequency of defined daily doses,DDDs)的相关性。结果:我院2018年至2021年抗菌药物DDDs较高的抗菌药物依次为美洛西林、头孢他啶、哌拉西林他唑巴坦,检出率较高的病原菌分别为大肠埃希氏菌、肺炎克雷伯菌、金黄色葡萄球菌等。大肠埃希菌产生的耐药率与哌拉西林他唑巴坦、头孢呋辛钠DDDs均有相关性(r=-0.107、0.432);肺炎克雷伯菌产生的耐药率与哌拉西林他唑巴坦、左氧氟沙星氯化钠、头孢呋辛钠DDDs呈正相关(r=0.410、0.720、0.420);铜绿假单胞菌与哌拉西林他唑巴坦、头孢哌酮舒巴坦钠DDDs呈正相关(r=0.716、0.933);金黄色葡萄球菌的耐药率与左氧氟沙星DDDs呈正相关(r=0.661);肺炎链球菌产生的耐药率与左氧氟沙星、头孢呋辛钠DDDs均存在正相关关系(r=0.416和0.482)。结论:抗菌药物的DDDs与常见细菌产生的耐药性具有一定的相关性,我院应严格控制抗菌药物的DDDs,根据感染性病种及药敏试验结果选择适当的抗菌药,加强常见病原菌的耐药监测。
文摘The different investigation has been carried out on the biological activities of titanium dioxide nanoparticle but the effect of this nano product on the antibacterial activity of different antibiotics has not been yet demonstrated. In this study the nano size TiO2 is synthesized using citric acid and alpha dextrose and the enhancement effect of TiO2 nanoparticle on the antibacterial activity of different antibiotics was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA). During the present study, different concentrations of nano-scale TiO2 were tested to find out the best concentration that can have the most effective antibacterial property against the MRSA culture. Disk diffusion method was used to determine the antibacterial activity of these antibiotics in the absence and presence of sub inhibitory concentration of TiO2 nano particle. A clinical isolate of MRSA, isolated from Intensive Care Unit (ICU) was used as test strain. In the presence of sub-inhibitory concentration of TiO2 nanoparticle (20 μg/disc) the antibacterial activities of all antibiotics have been increased against test strain with minimum 2 mm to maximum 10mm. The highest increase in inhibitory zone for MRSA was observed against pencillin G and amikacin (each 10 mm). Conversely, in case of nalidixic acid, TiO2 nanoparticle showed a Synergic effect on the antibacterial activity of this antibiotic against test strain. These results signify that the TiO2 nanoparticle potentate the antimicrobial action of beta lactums, cephalosporins, aminoglycosides, glycopeptides, macrolids and lincosamides, tetracycline a possible utilization of nano compound in combination effect against MRSA.
文摘Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the study, the exonic and 3’UTR sequences of five genes in 12 sporadic colorectal tumors were extracted by next generation sequencing. In tumors with variation in the 3’UTR region, the changes caused by the variation in the miRNA binding profile were detected. The expression profile of these miRNAs in colorectal and other solid tumors compared to normal tissue was determined. Pathway analysis was performed to determine which signaling pathways miRNAs affect. Results: Case-10 in our study was wild type KRAS and received cetuximab treatment and developed drug resistance. In this case, it was concluded that the expression of KRAS increased and tumorigenesis progressed due to miRNAs that do not bind to this region due to variations in the 3’UTR region. Among these miRNAs, hsa-miR-124-3p was found to have decreased expression in colorectal tumors and to be associated with the ECM-receptor interaction pathway. Conclusion: Variations in the 3’UTR regions of genes critical in the process of carsinogenesis are associated with drug resistance and the process of tumorigenesis.
文摘Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic bacteria identification and drug sensitivity tests were performed with a VITEK 2 compact automatic identification system and data were analyzed using WHONET5.6 software.Results： Of the 1,378 strains tested, 980 were Gram-negative bacilli, accounting for 71.1%, in which Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa were the dominant strains. We found 328 Gram-positive coccus, accounting for 23.8%, in which the amount of Staphylococcus aureus was the highest. We identified 46 fungi, accounting for 4.1%. According to the departmental distribution within the hospital, the surgical departments isolated the major strains, accounting for 49.7%. According to disease types, lung cancer, intestinal cancer and esophagus cancer were the top three, accounting for 20.9%, 17.3% and 14.2%, respectively. No strains were resistant to imipenem, ertapenem or vancomycin.Conclusions： Pathogenic bacteria isolated from the specialized cancer hospital have different resistance rates compared to commonly used antimicrobial agents; therefore antimicrobial agents to reduce the morbidity and mortality of infections should be used.
文摘Objective:To provide evidence for a rational and effective prevention and treatment of Pseudomonas aeruginosa,the clinical characteristics and the resistance to various antibiotics of were investigated.Methods:A retrospective analysis of 224 strains of Pseudomonas aeruginosa isolated from various specimens from various clinical departments of our hospital(April 1,2018 to June 31,2019)were conducted.Identification and drug susceptibility test of isolated strains was performed using a fully automatic bacterial identification analyzer(MicroScan WalkAway-96 plus),and data analysis was performed using WH0NET5.6 software.Results:Among all the bacteria isolated in our hospital during the above period,Pseudomonas aeruginosa accounted for 10.09% of them all and 12.57% of Gram-negative bacilli,respectively.These isolates were mainly derived from sputum specimens(68.75%),mainly from male patients(70.54%),and mostly 61-70(27.23%)or 51-60(22.77%)years old.Pseudomonas aeruginosa isolates are mainly from Rehabilitation Ward,ICU,and Liver Transplantation Unit,accounted for 29.91%,12.95% and 10.27% of all isolates,respectively.The sensitivity of Pseudomonas aeruginosa to various antibacterial drugs,in the order of high to low were carbapenems,aztreonam,quinolones,cephalosporins,piperacillin/tazobactam,aminoglycoside,with a lowest resistance rate(2.4%)to amikacin and a highest resistance rate to imipenem(33.0%).Conclusion:The isolation rate of Pseudomonas aeruginosa was relatively stable during the study period,and among all the P.aeruginosa detected,most of them were from the respiratory secretions of elderly male patients.The resistance rate of Pseudomonas aeruginosa isolates to various antibiotics is mainly within 30%.Clinical units such as Rehabilitation Ward,ICU,and Liver Transplantation Unit have a high detection rate;therefore,these departments should be monitored in a focused manner.Our research provides a scientific basis for the rational use of antibiotics and a better control of Pseudomonas aeruginosa infection.
文摘Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatment for the infection of Klebsiella pneumoniae.Methods:1,192 strains of Klebsiella pneumoniae isolated from clinical specimens from 2012 to 2016 were collected.The strains were identified by VITEK-2 Compact Microbiological Identification System,and the corresponding results of the antimicrobial susceptibility test were interpreted in accordance with the standards recommended by Clinical and Laboratory Standards Institute(CLSI).Results:1,192 strains of Klebsiella pneumoniae were mainly isolated from sputum(65.6%),and most of them were from Respiratory Medicine Department and Medical Intensive Care Unit of Respiratory Medicine Department(MICU),accounting for 41.4%.Out of 1,192 strains,448 strains were detected to produce extended-spectrum beta-lactamases(ESBLs),accounting for 37.6%.In addition,the detection rates of ESBL-producing Klebsiella pneumoniae for 5 consecutive years showed an increasing trend year by year,and they were higher than the national average values published by China Antimicrobial Resistance Surveillance System(CARSS)in the corresponding period.The drug resistance rate of ESBL-producing Klebsiella pneumoniae was significantly higher than that of non ESBL-producing strains.Conclusions:The infection caused by Klebsiella pneumoniae mainly occurs in the lower respiratory tract,and the drug resistance rates of Klebsiella pneumoniae to antibiotics in the drug susceptibility spectrum are maintained at a high level.Therefore,the rational selection of antibiotics for the clinical treatment of lower respiratory tract infection caused by Klebsiella pneumoniae must be based on the production of ESBLs and the results of antimicrobial susceptibility test.
