The objective of this study was to investigate the influences of organic solvents on particle size, drug content, loading efficiency and yield for 5 Fluorouracil Poly(lactic acid) nanoparticles . The 5 Fluorouracil wa...The objective of this study was to investigate the influences of organic solvents on particle size, drug content, loading efficiency and yield for 5 Fluorouracil Poly(lactic acid) nanoparticles . The 5 Fluorouracil was entrapped into poly(lactic acid)(PLA) nanoparticles using a water in oil in water solvent evaporation technique. During the preparation process, ethyl acetate and acetone were used as organic solvents since they are less toxic than the more commonly used dichloromethane. The effect of the three solvents on particle size, drug content, loading efficiency and yield of nanopartcles was compared. When the solvent of the oil phase was acetone, the highest drug content, smallest particle size and lowest yield were obtained for the PLA nanoparticles.展开更多
Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this ap...Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this approach was evaluated in terms of drug loading content （DLC）, encapsulation efficiency （EE） and delivery properties in vitro, determined by high performance liquid chromatograph （HPLC）. The microspheres showed good DLC values of 11.8%, as well as good EE values of 79.4%. The in vitro drug release study was carried out in phosphate buffer solution （PBS） simulated body fluid, at 37 ~C with pH=7.4. The release profiles showed an initial fast release rate, which decreased as time progressed and about 84 % had been released after 48 h. The experimental results indicated that the prepared magnetic microspheres may be useful for potential applications of MER for magnetically targeted chemotherapy.展开更多
Low drug loading efficiency is one of the main obstacles hindering the application of contact lenses(CLs) as the carrier for extended ocular drug delivery. Here in this study, a simple and effective drug loading metho...Low drug loading efficiency is one of the main obstacles hindering the application of contact lenses(CLs) as the carrier for extended ocular drug delivery. Here in this study, a simple and effective drug loading method based on salt induced modulation was proposed and demonstrated with mechanism elucidation. First of all, using poly(2-hydroxyethyl methacrylate)(p-HEMA) as the contact lens material, betaxolol hydrochloride, Diclofenac Sodium and Betaxolol Base as the model drugs with different solubility, influence of salt concentration,salt type(sodium salts of sulfate, chloride, and sulfocyanate) and drug properties in the loading solution on drug loading efficiency was investigated. Mechanism of enhanced drug loading in contact lens was further explored via studying the influence of salt on the absorption isotherm, drug solubility and water content of CLs. Applicability of this method to other CLs materials was also investigated. It was demonstrated that adjusting the ionic strength of loading solutions resulted in significant increase of drug loading in CLs. Type and concentration of the salts and solubility of the drug were the main factors influencing enhancement ratio of drug loading. The mechanism for improved drug loading was related to the reduced drug solubility in loading solutions and the reduced bound water content in contact lenses. Modulation of drug loading by adjusting ionic strength was also applicable to other CLs and the light transmittance was not affected. This method was more suitable for salt-form drugs with high solubility. In summary, adjusting ionic strength of loading solution is an economical and effective way to improve drug loading in CLs, and this simple method may also find application in other hydrogel based drug delivery systems.展开更多
Objective:To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha(Albezzia lebbeck),Nagarmotha(Cyprus ratandus) & Kantakari(Solanum xantho...Objective:To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha(Albezzia lebbeck),Nagarmotha(Cyprus ratandus) & Kantakari(Solanum xanthocarpum).Methods:Antimicrobial activity was evaluated by discdiffusion method and MIC,MBC,MFC were calculated by micro dilution method.Hydroalcholic extract of this preparation was investigated for its phytochemical analysis,phenol and Oavonoid were determined by speclrophotometric method and in vivo bronchodilator effect was analysed by convulsion time.Results:The phytochemical tests revealed presence of alkaloids, anthraquinones,carbohydrates,flavonoids,saponins and tannins.The antimicrobial result showed the MIC of 6.25 mg/ml.against Staphylococcus aureus and 12.5 mg/mL.for Escherichia coli and 12.5 mg/mL against remaining bacteria tested,with strong antifungal activity.The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL Drug showed significant bronchodilator effect with 27.86%& 36.13%increase in preconvulsion time of guinea pigs prebeated with 100 & 200 mg/kg body weight of extract.Conclusions:The study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins,further studies are highly needed for the drug development.展开更多
Thermosensitive liposomes(TSLs) have been an important research area in the field of tumor targeted chemotherapy. Since the first TSLs appeared that using 1,2-dipalmitoyl-snglyce-ro-3-phosphocholine(DPPC) as the prima...Thermosensitive liposomes(TSLs) have been an important research area in the field of tumor targeted chemotherapy. Since the first TSLs appeared that using 1,2-dipalmitoyl-snglyce-ro-3-phosphocholine(DPPC) as the primary liposomal lipid, many studies have been done using this type of liposome from basic and practical aspects. While TSLs composed of DPPC enhance the cargo release near the phase transition temperature, it has been shown that many factors affect their temperature sensitivity. Thus numerous attempts have been undertaken to develop new TSLs for improving their thermal response performance. The main objective of this review is to introduce the development and recent update of innovative TSLs formulations, including combination of radiofrequency ablation(RFA), highintensity focused ultrasound(HIFU), magnetic resonance imaging(MRI) and alternating magnetic field(AMF). In addition, various factors affecting the design of TSLs, such as lipid composition, surfactant, size and serum components are also discussed.展开更多
Objective: To explore the leaves of Hydrocotyle javanica Thunb. as a source of safe and effective antibacterial herbal medicine.Methods: The standardization was validated by stepwise physicochemical studies,element an...Objective: To explore the leaves of Hydrocotyle javanica Thunb. as a source of safe and effective antibacterial herbal medicine.Methods: The standardization was validated by stepwise physicochemical studies,element analysis, determination of ash values, fluorescence analysis, assessment of moisture content, extractive values in different solvent systems and extraction methods.Heavy metal contents, mineral and element contents were analysed by atomic absorption spectrophotometry, inductively coupled plasma-mass spectrometer and CHNS/O analyser, respectively.Results: The methanol extract of the folklore medicinal plant having antibacterial efficacy contained flavonoids and phenolic OH groups. The ICP multi standard indicated the presence of three major compounds with molecular mass of 161 190 and 221 Da. Heavy metals viz. lead, mercury and copper content were 4.38 ppm, < 0.05 ppm and 24.70 ppm,respectively. Minerals content of calcium, phosphorus, potassium and iron were 1 190.94 mg/100 g, 375.57 mg/100 g, 2 820 mg/100 g and 340.20 mg/100 g of plant sample, respectively. Elements like carbon, hydrogen, nitrogen and sulphur contents were38.18%, 5.67%, 2.23% and 0.51%, respectively. Heavy metal profile of the tested plant was within the permissible limits of the regulatory authorities.Conclusions: Hence the present physicochemical and elements studies reveals that the plant Hydrocotyle javanica Thunb. could be a potent source of herbal preparation as well as a safe and novel synthetic antibacterial drug.展开更多
文摘The objective of this study was to investigate the influences of organic solvents on particle size, drug content, loading efficiency and yield for 5 Fluorouracil Poly(lactic acid) nanoparticles . The 5 Fluorouracil was entrapped into poly(lactic acid)(PLA) nanoparticles using a water in oil in water solvent evaporation technique. During the preparation process, ethyl acetate and acetone were used as organic solvents since they are less toxic than the more commonly used dichloromethane. The effect of the three solvents on particle size, drug content, loading efficiency and yield of nanopartcles was compared. When the solvent of the oil phase was acetone, the highest drug content, smallest particle size and lowest yield were obtained for the PLA nanoparticles.
基金Funded by the Natural Science Foundation of Hubei Province(No.2011CDA056)the International Cooperation Funding of Hubei Province(No.2012IHA0120)+1 种基金the Fundamental Research Funds for the Central Universities of China(2012-IV-029)the Undergraduate innovation Funding of Wuhan University of Technology(136620004)
文摘Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this approach was evaluated in terms of drug loading content （DLC）, encapsulation efficiency （EE） and delivery properties in vitro, determined by high performance liquid chromatograph （HPLC）. The microspheres showed good DLC values of 11.8%, as well as good EE values of 79.4%. The in vitro drug release study was carried out in phosphate buffer solution （PBS） simulated body fluid, at 37 ~C with pH=7.4. The release profiles showed an initial fast release rate, which decreased as time progressed and about 84 % had been released after 48 h. The experimental results indicated that the prepared magnetic microspheres may be useful for potential applications of MER for magnetically targeted chemotherapy.
基金supported by the Distinguished Professor Project of Liaoning Province(2015)
文摘Low drug loading efficiency is one of the main obstacles hindering the application of contact lenses(CLs) as the carrier for extended ocular drug delivery. Here in this study, a simple and effective drug loading method based on salt induced modulation was proposed and demonstrated with mechanism elucidation. First of all, using poly(2-hydroxyethyl methacrylate)(p-HEMA) as the contact lens material, betaxolol hydrochloride, Diclofenac Sodium and Betaxolol Base as the model drugs with different solubility, influence of salt concentration,salt type(sodium salts of sulfate, chloride, and sulfocyanate) and drug properties in the loading solution on drug loading efficiency was investigated. Mechanism of enhanced drug loading in contact lens was further explored via studying the influence of salt on the absorption isotherm, drug solubility and water content of CLs. Applicability of this method to other CLs materials was also investigated. It was demonstrated that adjusting the ionic strength of loading solutions resulted in significant increase of drug loading in CLs. Type and concentration of the salts and solubility of the drug were the main factors influencing enhancement ratio of drug loading. The mechanism for improved drug loading was related to the reduced drug solubility in loading solutions and the reduced bound water content in contact lenses. Modulation of drug loading by adjusting ionic strength was also applicable to other CLs and the light transmittance was not affected. This method was more suitable for salt-form drugs with high solubility. In summary, adjusting ionic strength of loading solution is an economical and effective way to improve drug loading in CLs, and this simple method may also find application in other hydrogel based drug delivery systems.
文摘Objective:To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha(Albezzia lebbeck),Nagarmotha(Cyprus ratandus) & Kantakari(Solanum xanthocarpum).Methods:Antimicrobial activity was evaluated by discdiffusion method and MIC,MBC,MFC were calculated by micro dilution method.Hydroalcholic extract of this preparation was investigated for its phytochemical analysis,phenol and Oavonoid were determined by speclrophotometric method and in vivo bronchodilator effect was analysed by convulsion time.Results:The phytochemical tests revealed presence of alkaloids, anthraquinones,carbohydrates,flavonoids,saponins and tannins.The antimicrobial result showed the MIC of 6.25 mg/ml.against Staphylococcus aureus and 12.5 mg/mL.for Escherichia coli and 12.5 mg/mL against remaining bacteria tested,with strong antifungal activity.The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL Drug showed significant bronchodilator effect with 27.86%& 36.13%increase in preconvulsion time of guinea pigs prebeated with 100 & 200 mg/kg body weight of extract.Conclusions:The study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins,further studies are highly needed for the drug development.
基金National Natural Science Foundation of China (No.31671020) for financial support
文摘Thermosensitive liposomes(TSLs) have been an important research area in the field of tumor targeted chemotherapy. Since the first TSLs appeared that using 1,2-dipalmitoyl-snglyce-ro-3-phosphocholine(DPPC) as the primary liposomal lipid, many studies have been done using this type of liposome from basic and practical aspects. While TSLs composed of DPPC enhance the cargo release near the phase transition temperature, it has been shown that many factors affect their temperature sensitivity. Thus numerous attempts have been undertaken to develop new TSLs for improving their thermal response performance. The main objective of this review is to introduce the development and recent update of innovative TSLs formulations, including combination of radiofrequency ablation(RFA), highintensity focused ultrasound(HIFU), magnetic resonance imaging(MRI) and alternating magnetic field(AMF). In addition, various factors affecting the design of TSLs, such as lipid composition, surfactant, size and serum components are also discussed.
文摘Objective: To explore the leaves of Hydrocotyle javanica Thunb. as a source of safe and effective antibacterial herbal medicine.Methods: The standardization was validated by stepwise physicochemical studies,element analysis, determination of ash values, fluorescence analysis, assessment of moisture content, extractive values in different solvent systems and extraction methods.Heavy metal contents, mineral and element contents were analysed by atomic absorption spectrophotometry, inductively coupled plasma-mass spectrometer and CHNS/O analyser, respectively.Results: The methanol extract of the folklore medicinal plant having antibacterial efficacy contained flavonoids and phenolic OH groups. The ICP multi standard indicated the presence of three major compounds with molecular mass of 161 190 and 221 Da. Heavy metals viz. lead, mercury and copper content were 4.38 ppm, < 0.05 ppm and 24.70 ppm,respectively. Minerals content of calcium, phosphorus, potassium and iron were 1 190.94 mg/100 g, 375.57 mg/100 g, 2 820 mg/100 g and 340.20 mg/100 g of plant sample, respectively. Elements like carbon, hydrogen, nitrogen and sulphur contents were38.18%, 5.67%, 2.23% and 0.51%, respectively. Heavy metal profile of the tested plant was within the permissible limits of the regulatory authorities.Conclusions: Hence the present physicochemical and elements studies reveals that the plant Hydrocotyle javanica Thunb. could be a potent source of herbal preparation as well as a safe and novel synthetic antibacterial drug.
