Objective: The efficacy of Ji Desheng snake pills combined with hypertonic glucose external application in treating drug-induced superficial phlebitis caused by parenteral nutrition (PN) is observed. Methods: Fifty-tw...Objective: The efficacy of Ji Desheng snake pills combined with hypertonic glucose external application in treating drug-induced superficial phlebitis caused by parenteral nutrition (PN) is observed. Methods: Fifty-two cases of drug-induced superficial phlebitis after peripheral parenteral nutrition (PPN) were selected, which were randomly divided into experimental group and control group in accordance with the phlebitis grading. In the experimental group, Ji Desheng snake pills were crushed to make a paste with 50% glucose solution, which was then applied to the affected area of phlebitis, the surface was covered with clean gauze, and properly fixed with tape or bandage. The drug was replaced once a day. In the control group, the gauze soaked with 50% magnesium sulfate solution was used, which was applied to the affected part three times a day in wet, and the efficacy was observed respectively on the 1st, 3rd, 5th and 7th days after applying the drug. Results: On the 1st and 3rd days after treatment, the observed effective rate of the experimental group was higher than that of the control group (42.31% vs. 15.38% and 76.92% vs. 46.15%, respectively). The difference was statistically significant (p th and 7th days after treatment, there was no statistical significance with respect to the efficacy between the experimental group and the control group (p > 0.05). Conclusion: The significant efficacy could be found in early stage after drug-induced superficial phlebitis was treated by Ji Desheng snake pills combined with hypertonic glucose external application, which was superior to that of the traditional treatment of wet application by using gauze soaked in 50% magnesium sulfate solution.展开更多
The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was ...The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation assay and contact insecticidal effect assay, were carried out separately to verify the toxicity of this recombinant toxin. At the end of a 24 h experimental period, more than 60% of the testing diamondback moth (Plutella xylostella) larvae were killed in both groups with LC50 value of 18.4 microM and 0.70 microM respectively. Cytotoxicity assay using cultured Sf9 insect cells and MCF-7 human cells demonstrated that the toxin AaIT had specific toxicity against insect cells but not human cells. Only 0.13 microM recombinant toxin was needed to kill 50% of cultured insect cells while as much as 1.3 microM toxin had absolutely no effect on human cells. Insect cells produced obvious intrusions from their plasma membrane before broken up. We infer that toxin AaIT bind to a putative sodium channel in these insect cells and open the channel persistently, which would result in Na+ influx and finally cause destruction of insect cells.展开更多
为了同时监管禽肉中多种类型(四咪唑类、硝基咪唑类、三嗪类、氨基甲酸酯类和有机氮类)抗虫药,提出了题示研究。取已研磨混匀的样品(2±0.01)g,加入5 m L水和10 m L提取剂(由1 m L乙酸、600 m L乙腈和400 m L乙酸乙酯组成),涡旋振荡...为了同时监管禽肉中多种类型(四咪唑类、硝基咪唑类、三嗪类、氨基甲酸酯类和有机氮类)抗虫药,提出了题示研究。取已研磨混匀的样品(2±0.01)g,加入5 m L水和10 m L提取剂(由1 m L乙酸、600 m L乙腈和400 m L乙酸乙酯组成),涡旋振荡20 min,加入无水Mg SO40.5 g,离心5 min。取5 m L上层液于40℃氮气吹干,在净化管中加入20 mg C18粉、30 mg N-丙基乙二胺(PSA)粉和1 m L定容液(由1 m L甲酸、900 m L去离子水和100 m L乙腈组成)后涡旋1 min,用0.22μm双系微孔滤膜过滤。以GISTC18-AQ色谱柱(100 mm×2.1 mm,1.9μm)为固定相,以不同体积比的0.1%(体积分数)甲酸溶液-乙腈混合液为流动相梯度洗脱分离滤液中24种抗虫药。质谱分析采用多反应监测模式,基质匹配法定量。结果表明,24种抗虫药的质量浓度在一定范围内与对应的质谱峰面积呈线性关系,检出限(3S/N)为0.01~0.40μg·kg^(-1)。按照标准加入法进行回收试验,回收率为61.2%~113%,测定值的相对标准偏差(n=6)不大于15%。方法用于分析30份实际样品,仅在1份鸭肉样品中检出羟基二甲硝咪唑,检出量为1.46μg·kg^(-1),与农业农村部公告第197号-10-2019、SN/T 4809—2017标准方法中的测定结果基本一致。展开更多
文摘Objective: The efficacy of Ji Desheng snake pills combined with hypertonic glucose external application in treating drug-induced superficial phlebitis caused by parenteral nutrition (PN) is observed. Methods: Fifty-two cases of drug-induced superficial phlebitis after peripheral parenteral nutrition (PPN) were selected, which were randomly divided into experimental group and control group in accordance with the phlebitis grading. In the experimental group, Ji Desheng snake pills were crushed to make a paste with 50% glucose solution, which was then applied to the affected area of phlebitis, the surface was covered with clean gauze, and properly fixed with tape or bandage. The drug was replaced once a day. In the control group, the gauze soaked with 50% magnesium sulfate solution was used, which was applied to the affected part three times a day in wet, and the efficacy was observed respectively on the 1st, 3rd, 5th and 7th days after applying the drug. Results: On the 1st and 3rd days after treatment, the observed effective rate of the experimental group was higher than that of the control group (42.31% vs. 15.38% and 76.92% vs. 46.15%, respectively). The difference was statistically significant (p th and 7th days after treatment, there was no statistical significance with respect to the efficacy between the experimental group and the control group (p > 0.05). Conclusion: The significant efficacy could be found in early stage after drug-induced superficial phlebitis was treated by Ji Desheng snake pills combined with hypertonic glucose external application, which was superior to that of the traditional treatment of wet application by using gauze soaked in 50% magnesium sulfate solution.
基金This work was supported by a grant from 863High Technology Program,Chinese Ministry of Sci-ence and Technology
文摘The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation assay and contact insecticidal effect assay, were carried out separately to verify the toxicity of this recombinant toxin. At the end of a 24 h experimental period, more than 60% of the testing diamondback moth (Plutella xylostella) larvae were killed in both groups with LC50 value of 18.4 microM and 0.70 microM respectively. Cytotoxicity assay using cultured Sf9 insect cells and MCF-7 human cells demonstrated that the toxin AaIT had specific toxicity against insect cells but not human cells. Only 0.13 microM recombinant toxin was needed to kill 50% of cultured insect cells while as much as 1.3 microM toxin had absolutely no effect on human cells. Insect cells produced obvious intrusions from their plasma membrane before broken up. We infer that toxin AaIT bind to a putative sodium channel in these insect cells and open the channel persistently, which would result in Na+ influx and finally cause destruction of insect cells.
文摘为了同时监管禽肉中多种类型(四咪唑类、硝基咪唑类、三嗪类、氨基甲酸酯类和有机氮类)抗虫药,提出了题示研究。取已研磨混匀的样品(2±0.01)g,加入5 m L水和10 m L提取剂(由1 m L乙酸、600 m L乙腈和400 m L乙酸乙酯组成),涡旋振荡20 min,加入无水Mg SO40.5 g,离心5 min。取5 m L上层液于40℃氮气吹干,在净化管中加入20 mg C18粉、30 mg N-丙基乙二胺(PSA)粉和1 m L定容液(由1 m L甲酸、900 m L去离子水和100 m L乙腈组成)后涡旋1 min,用0.22μm双系微孔滤膜过滤。以GISTC18-AQ色谱柱(100 mm×2.1 mm,1.9μm)为固定相,以不同体积比的0.1%(体积分数)甲酸溶液-乙腈混合液为流动相梯度洗脱分离滤液中24种抗虫药。质谱分析采用多反应监测模式,基质匹配法定量。结果表明,24种抗虫药的质量浓度在一定范围内与对应的质谱峰面积呈线性关系,检出限(3S/N)为0.01~0.40μg·kg^(-1)。按照标准加入法进行回收试验,回收率为61.2%~113%,测定值的相对标准偏差(n=6)不大于15%。方法用于分析30份实际样品,仅在1份鸭肉样品中检出羟基二甲硝咪唑,检出量为1.46μg·kg^(-1),与农业农村部公告第197号-10-2019、SN/T 4809—2017标准方法中的测定结果基本一致。
