<strong>Background:</strong> This work evaluated the capacity of a dry emulsion as a carrier of viable microorganisms with potential use as prophylaxis of infectious diseases. <strong>Methods:</st...<strong>Background:</strong> This work evaluated the capacity of a dry emulsion as a carrier of viable microorganisms with potential use as prophylaxis of infectious diseases. <strong>Methods:</strong> The aqueous phase containing <em>P. multocida </em>not viable in PBS was emulsified in mineral oil to obtain a w/o emulsion. The microorganisms remained stable and only in two cases (n = 6) did the bacterial concentration decrease. Scanning Electron Microscopy (SEM) revealed a structure of a system with the organized association of particles with cubic symmetry. Using two <em>ex vivo </em>bioadhesion systems, it was demonstrated that the disperse-adsorbed system is capable of adhering to the intestinal mucosa and remains adhered for long periods of time. <strong>Results: </strong>The no viability of the bacteria in the dry emulsion and the possibility of controlled release were confirmed. <em>In vivo </em>trial was conducted in pigs. It was possible to locate the emulsion and the bacteria attached to the gut of the living animal. An ELISA kit was used to monitor the mean antibody titer of treated pigs over a 2-week period, and a classic primary response curve occurred when the titer was plotted against time. <strong>Conclusion: </strong>We propose the disperse-adsorbed system as an alternative to commonly used vehicles for immunogens in the oral vaccines.展开更多
文摘<strong>Background:</strong> This work evaluated the capacity of a dry emulsion as a carrier of viable microorganisms with potential use as prophylaxis of infectious diseases. <strong>Methods:</strong> The aqueous phase containing <em>P. multocida </em>not viable in PBS was emulsified in mineral oil to obtain a w/o emulsion. The microorganisms remained stable and only in two cases (n = 6) did the bacterial concentration decrease. Scanning Electron Microscopy (SEM) revealed a structure of a system with the organized association of particles with cubic symmetry. Using two <em>ex vivo </em>bioadhesion systems, it was demonstrated that the disperse-adsorbed system is capable of adhering to the intestinal mucosa and remains adhered for long periods of time. <strong>Results: </strong>The no viability of the bacteria in the dry emulsion and the possibility of controlled release were confirmed. <em>In vivo </em>trial was conducted in pigs. It was possible to locate the emulsion and the bacteria attached to the gut of the living animal. An ELISA kit was used to monitor the mean antibody titer of treated pigs over a 2-week period, and a classic primary response curve occurred when the titer was plotted against time. <strong>Conclusion: </strong>We propose the disperse-adsorbed system as an alternative to commonly used vehicles for immunogens in the oral vaccines.
