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Alternating current heating techniques for lithium-ion batteries in electric vehicles:Recent advances and perspectives
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作者 Xinrong Huang Jinhao Meng +5 位作者 Wei Jiang Wenjie Liu Kailong Liu Yipu Zhang Daniel-Ioan Stroe Remus Teodorescu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第9期679-697,共19页
The significant decrease in battery performance at low temperatures is one of the critical challenges that electric vehicles(EVs)face,thereby affecting the penetration rate in cold regions.Alternating current(AC)heati... The significant decrease in battery performance at low temperatures is one of the critical challenges that electric vehicles(EVs)face,thereby affecting the penetration rate in cold regions.Alternating current(AC)heating has attracted widespread attention due to its low energy consumption and uniform heating advantages.This paper introduces the recent advances in AC heating from the perspective of practical EV applications.First,the performance degradation of EVs in low-temperature environments is introduced briefly.The concept of AC heating and its research methods are provided.Then,the effects of various AC heating methods on battery heating performance are reviewed.Based on existing studies,the main factors that affect AC heating performance are analyzed.Moreover,various heating circuits based on EVs are categorized,and their cost,size,complexity,efficiency,reliability,and heating rate are elaborated and compared.The evolution of AC heaters is presented,and the heaters used in brand vehicles are sorted out.Finally,the perspectives and challenges of AC heating are discussed.This paper can guide the selection of heater implementation methods and the optimization of heating effects for future EV applications. 展开更多
关键词 Lithium-ion battery Low temperature alternating current heating HEATER Electric vehicle
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Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain
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作者 Yi Jiang Wenli Tao +2 位作者 Weiyang Zhang Zhiqin Wang Jianchang Yang 《The Crop Journal》 SCIE CSCD 2024年第1期262-270,共9页
Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breedin... Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG. 展开更多
关键词 BRASSINOSTEROIDS Inferior grain Lysine biosynthesis Rice Wetting alternating with partial drying
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Suppression of stimulated Brillouin and Raman scatterings using an alternating frequency laser and transverse magnetic fields
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作者 程瑞锦 李晓旬 +11 位作者 王清 刘德基 黄卓明 吕帅宇 周远志 张舒童 李雪铭 陈祖杰 王强 刘占军 曹莉华 郑春阳 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期545-553,共9页
A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allow... A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allows the laser frequency to change discretely and alternately over time. The suppression of SBS is significant as long as the AF difference is greater than the linear growth rate of SBS or the alternating time of the laser frequency is shorter than the linear growth time of SBS. However, the AF laser proves ineffective in suppressing SRS, which usually has a much higher linear growth rate than SBS. To remedy that, a transverse magnetic field is included to suppress the SRS instability. The electrons trapped in the electron plasma waves(EPWs) of SRS can be accelerated by the surfatron mechanism in a transverse magnetic field and eventually detrapped. While continuously extracting energy from EPWs, the EPWs are dissipated and the kinetic inflation of SRS is suppressed. The one-dimensional particle-in-cell simulation results show that both SBS and SRS can be effectively suppressed by combining the AF laser with a transverse magnetic field with tens of Tesla. The total reflectivity can be dramatically reduced by more than one order of magnitude. These results provide a potential reference for controlling SBS and SRS under the related parameters of inertial confinement fusion. 展开更多
关键词 stimulated Brillouin scattering stimulated Raman scattering alternating frequency laser transverse magnetic field
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Diagnosing ratio of electron density to collision frequency of plasma surrounding scaled model in a shock tube using low-frequency alternating magnetic field phase shift
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作者 吴明兴 谢楷 +3 位作者 刘艳 徐晗 张宝 田得阳 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第5期519-530,共12页
A non-contact low-frequency(LF)method of diagnosing the plasma surrounding a scaled model in a shock tube is proposed.This method utilizes the phase shift occurring after the transmission of an LF alternating magnetic... A non-contact low-frequency(LF)method of diagnosing the plasma surrounding a scaled model in a shock tube is proposed.This method utilizes the phase shift occurring after the transmission of an LF alternating magnetic field through the plasma to directly measure the ratio of the plasma loop average electron density to collision frequency.An equivalent circuit model is used to analyze the relationship of the phase shift of the magnetic field component of LF electromagnetic waves with the plasma electron density and collision frequency.The applicable range of the LF method on a given plasma scale is analyzed.The upper diagnostic limit for the ratio of the electron density(unit:m^(-3))to collision frequency(unit:Hz)exceeds 1×10^(11),enabling an electron density to exceed 1×10^(20)m^(-3)and a collision frequency to be less than 1 GHz.In this work,the feasibility of using the LF phase shift to implement the plasma diagnosis is also assessed.Diagnosis experiments on shock tube equipment are conducted by using both the electrostatic probe method and LF method.By comparing the diagnostic results of the two methods,the inversion results are relatively consistent with each other,thereby preliminarily verifying the feasibility of the LF method.The ratio of the electron density to the collision frequency has a relatively uniform distribution during the plasma stabilization.The LF diagnostic path is a loop around the model,which is suitable for diagnosing the plasma that surrounds the model.Finally,the causes of diagnostic discrepancy between the two methods are analyzed.The proposed method provides a new avenue for diagnosing high-density enveloping plasma. 展开更多
关键词 low-frequency alternating magnetic field phase shift shock-tube plasma diagnosis electron density collision frequency
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Mechanism of plasticity enhancement of AZ31B magnesium alloy sheet by accumulative alternating back extrusion 被引量:3
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作者 Ye Wang Feng Li +2 位作者 Nan Bian Hua Qiu Du Peng Da Huo 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2023年第5期1791-1801,共11页
Accumulative alternating back extrusion was a potential fine-grain modification method.In this paper,it was an innovative attempt to develop high-performance magnesium alloy sheet by this process.Under the condition o... Accumulative alternating back extrusion was a potential fine-grain modification method.In this paper,it was an innovative attempt to develop high-performance magnesium alloy sheet by this process.Under the condition of 350 K,commercial AZ31 magnesium alloy was made into billet by accumulative alternating back extrusion,and then extruded into fine-grain magnesium alloy sheet.Through a systematic study of its microstructure and mechanical properties,the results showed that the initial state had an important influence on the evolution of the structure during extrusion.After accumulative alternating back extrusion to produce the billet,the grain size of the sheet obtained by extrusion was significantly refined,which was related to the accumulation of deformation and grain refinement during the alternating loading process.Grain refinement caused the proportion of dynamic recrystallization inside the sheet with 2 cycles of accumulative alternating back extrusion to drop to 27%.With the increase of extrusion cycles from 2 to 4,the high density of dislocations led to an increase in the proportion of dynamic recrystallization and finer grains.The texture changed from strong basal texture to weak bimodal texture.The results of uniaxial tensile test show that due to grain refinement and texture change,the yield strength was significantly reduced,and the plasticity was significantly improved.It was verified that accumulative alternating back extrusion was meaningful for subsequent processing,and it also provided scientific guidance for the development of fine-grained magnesium alloy sheet. 展开更多
关键词 Magnesium alloy alternating back extrusion Microstructure uniformity Bimodal texture Texture weakening Sheet anisotropy
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Texture property and weakening mechanism of Mg-3Al-1Zn alloy by interactive alternating forward extrusion
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作者 Ye Wang Feng Li +1 位作者 Yu Wang Xing Mao Xiao 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2023年第2期684-693,共10页
The interactive alternating forward extrusion(AFE) method can realize the change of texture type and the weakening of texture strength.Taking AZ31 magnesium alloy as an example, the texture evolution of interactive AF... The interactive alternating forward extrusion(AFE) method can realize the change of texture type and the weakening of texture strength.Taking AZ31 magnesium alloy as an example, the texture evolution of interactive AFE was studied. The results show that all kinds of dynamic recrystallization(DRX) behaviors can weaken the texture to vary degrees. The weakening effect of twinning-induced recrystallization(TDRX)behavior was particularly significant. During the interactive AFE process, the c-axis of most grains rotated under the external force, and tended to be 90° angle with the ED direction, forming a stable fiber texture. In addition, with the increase of loading passes, the starting of {0001} <11–20> basal slip system became more and more difficult. The(10–10) texture formed by {10–10} <11–20> prismatic slip system after sixth passes was the main texture type. With the increase of forming temperature, the starting ability of {10–10} <11–20>prismatic slip systems increased, and the(10–10) texture formed by prismatic slip system above 623 K dominated. 展开更多
关键词 Magnesium alloy alternating extrusion TEXTURE RECRYSTALLIZATION Grain orientation
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A Relationship between the Partial Bell Polynomials and Alternating Run Polynomials
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作者 Yanan Feng Zhe Wang 《Open Journal of Discrete Mathematics》 2023年第2期49-54,共6页
In this note, we first derive an exponential generating function of the alternating run polynomials. We then deduce an explicit formula of the alternating run polynomials in terms of the partial Bell polynomials.
关键词 alternating Run Polynomials Bell Polynomials PERMUTATIONS
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Low-complexity iterative equalization for OTFS based on alternating minimization
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作者 HE Xin JIA Haoxiang +2 位作者 SUN Yutong ZHOU Zijian ZHAO Danfeng 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第4期851-860,共10页
To achieve robust communication in high mobility scenarios,an iterative equalization algorithm based on alternating minimization(AM)is proposed for the orthogonal time frequency space(OTFS)system.The algorithm approxi... To achieve robust communication in high mobility scenarios,an iterative equalization algorithm based on alternating minimization(AM)is proposed for the orthogonal time frequency space(OTFS)system.The algorithm approximates the equalization problem to a convex function optimization problem in the real-valued domain and solves the problem iteratively using the AM algorithm.In the iterative process,the complexity of the proposed algorithm is reduced further based on the study of the cyclic structure and sparse property of the OTFS channel matrix in the delay-Doppler(DD)domain.The new method for OTFS is simulated and verified in a high-speed mobile scenario and the results show that the proposed equalization algorithm has excellent bit error rate performance with low complexity. 展开更多
关键词 orthogonal time frequency space(OTFS) alternating minimization(AM) high-speed mobile scenario iterative equalization
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Exploring the mechanical behavior and microstructure of compacted loess subjected to dry-wet cycles and chemical contamination
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作者 Yongpeng Nie Wankui Ni +1 位作者 Xiangfei Lü Wenxin Tuo 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第9期3673-3695,共23页
Due to climatic factors and rapid urbanization,the soil in the Loess Plateau,China,experiences the coupled effects of dry-wet cycles and chemical contamination.Understanding the mechanical behavior and corresponding m... Due to climatic factors and rapid urbanization,the soil in the Loess Plateau,China,experiences the coupled effects of dry-wet cycles and chemical contamination.Understanding the mechanical behavior and corresponding microstructural evolution of contaminated loess subjected to dry-wet cycles is essential to elucidate the soil degradation mechanism.Therefore,direct shear and consolidation tests were performed to investigate the variations in mechanical properties of compacted loess contaminated with acetic acid,sodium hydroxide,and sodium sulfate during dry-wet cycles.The mechanical response mechanisms were investigated using zeta potential,mineral chemical composition,and scanning electron microscopy(SEM)tests.The results indicate that the mechanical deterioration of sodium hydroxidecontaminated loess during dry-wet cycles decreases with increasing contaminant concentration,which is mainly attributed to the thickening of the electrical double layer(EDL)by Nat and the precipitation of calcite,as well as the formation of colloidal flocs induced by OH,thus inhibiting the development of large pores during the dry-wet process.In contrast,the attenuation of mechanical properties of both acetic acid-and sodium sulfate-contaminated loess becomes more severe with increasing contaminant concentration,with the latter being more particularly significant.This is primarily due to the reduction of the EDL thickness and the erosion of cement in the acidic environment,which facilitates the connectivity of pores during dry-wet cycles.Furthermore,the salt expansion generated by the drying process of saline loess further intensifies the structural disturbance.Consequently,the mechanical performance of compacted loess is sensitive to both pollutant type and concentration,exhibiting different response patterns in the dry-wet cycling condition. 展开更多
关键词 Contaminated loess dry-wet cycles COMPRESSIBILITY Shear strength Microstructural evolution
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Effects of dry-wet cycles on the mechanical properties of sandstone with unloading-induced damage
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作者 NAN Gan ZHANG Jiaming +2 位作者 LUO Yi WANG Xinlong HU Zhongyi 《Journal of Mountain Science》 SCIE CSCD 2024年第10期3474-3486,共13页
Sandstone is the fundamental material in various engineering and construction projects.However,the mechanical integrity of sandstone can be compromised by initial unloading damage resulting from activities such as eng... Sandstone is the fundamental material in various engineering and construction projects.However,the mechanical integrity of sandstone can be compromised by initial unloading damage resulting from activities such as engineering excavations.Furthermore,this degradation is further exacerbated under periodic dry-wet environmental conditions.This study investigated the effects of dry-wet cycles and unloading on the mechanical properties of jointed fine sandstone using uniaxial and triaxial compression tests.These tests were performed on rock samples subjected to varying unloading degrees and different numbers of dry-wet cycles.The results demonstrate that with an increase in the unloading degree from 0%to 70%,there is a corresponding decrease in peak stress ranging from 10%to 33%.Additionally,the cohesion exhibits a reduction of approximately 20%to 25%,while the internal friction angle experiences a decline of about 3.5%to 6%.These findings emphasize a significant unloading effect.Moreover,the degree of peak stress degradation in unloading jointed fine sandstone diminishes with an increase in confining pressure,suggesting that confining pressure mitigates the deterioration caused by dry-wet cycles.Additionally,as the number of dry-wet cycles increases,there is a notable decline in the mechanical properties of the sandstone,evidencing significant dry-wet degradation.Utilizing the Drucker Prager criterion,this study establishes a strength criterion and fracture criterion,denoted as σ_(1)(m,n) and K_(T)^(Ⅱ)(m, n), to quantify the combined impacts of dry-wet cycles and unloading on jointed fine sandstone,which provides a comprehensive understanding of its mechanical behavior under such conditions. 展开更多
关键词 UNLOADING dry-wet cycle Jointed fine sandstone Strength criterion fracture criterion Mechanical properties
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Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape
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作者 Hainan Liu Maosong Pei +5 位作者 Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi Dalong Guo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期863-875,共13页
The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the under... The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries. 展开更多
关键词 GRAPE postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)
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Alternate cotton-peanut intercropping:a new approach to increasing productivity and minimizing environmental impact
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作者 CHI Baojie DONG Hezhong 《Journal of Cotton Research》 CAS 2024年第1期101-103,共3页
Recent publications have highlighted the development of an alternate cotton-peanut intercropping as a novel strat-egy to enhance agricultural productivity.In this article,we provide an overview of the progress made in... Recent publications have highlighted the development of an alternate cotton-peanut intercropping as a novel strat-egy to enhance agricultural productivity.In this article,we provide an overview of the progress made in the alternate cotton-peanut intercropping,specifically focusing on its yield benefits,environmental impacts,and the underlying mechanisms.In addition,we advocate for future investigations into the selection or development of appropriate crop varieties and agricultural equipment,pest management options,and the mechanisms of root-canopy interactions.This review is intended to provide a valuable reference for understanding and adopting an alternate intercropping system for sustainable cotton production. 展开更多
关键词 COTTON PEANUT alternate intercropping PRODUCTIVITY
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Pile foundation in alternate layered liquefiable and non-liquefiable soil deposits subjected to earthquake loading
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作者 Praveen Huded M Suresh R Dash 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第2期359-376,共18页
Pile foundations are still the preferred foundation system for high-rise structures in earthquake-prone regions.Pile foundations have experienced failures in past earthquakes due to liquefaction.Research on pile found... Pile foundations are still the preferred foundation system for high-rise structures in earthquake-prone regions.Pile foundations have experienced failures in past earthquakes due to liquefaction.Research on pile foundations in liquefiable soils has primarily focused on the pile foundation behavior in two or three-layered soil profiles.However,in natural occurrence,it may occur in alternative layers of liquefiable and non-liquefiable soil.However,the experimental and/or numerical studies on the layered effect on pile foundations have not been widely addressed in the literature.Most of the design codes across the world do not explicitly mention the effect of sandwiched non-liquefiable soil layers on the pile response.In the present study,the behavior of an end-bearing pile in layered liquefiable and non-liquefiable soil deposit is studied numerically.This study found that the kinematic bending moment is higher and governs the design when the effect of the sandwiched non-liquefied layer is considered in the analysis as opposed to when its effect is ignored.Therefore,ignoring the effect of the sandwiched non-liquefied layer in a liquefiable soil deposit might be a nonconservative design approach. 展开更多
关键词 pile foundation LIQUEFACTION alternately layered soil fixity effect layered effect
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Effect of dry-wet cycles on dynamic properties and microstructures of sandstone:Experiments and modelling
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作者 Hai Pu Qingyu Yi +3 位作者 Andrey P.Jivkov Zhengfu Bian Weiqiang Chen Jiangyu Wu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第5期655-679,共25页
Underground pumped storage power plant(UPSP)is an innovative concept for space recycling of abandoned mines.Its realization requires better understanding of the dynamic performance and durability of reservoir rock.Thi... Underground pumped storage power plant(UPSP)is an innovative concept for space recycling of abandoned mines.Its realization requires better understanding of the dynamic performance and durability of reservoir rock.This paper conducted ultrasonic detection,split Hopkinson pressure bar(SHPB)impact,mercury intrusion porosimetry(MIP),and backscatter electron observation(BSE)tests to investigate the dynamical behaviour and microstructure of sandstone with cyclical dry-wet damage.A coupling FEM-DEM model was constructed for reappearing mesoscopic structure damage.The results show that dry-wet cycles decrease the dynamic compressive strength(DCS)with a maximum reduction of 39.40%,the elastic limit strength is reduced from 41.75 to 25.62 MPa.The sieved fragments obtain the highest crack growth rate during the 23rd dry-wet cycle with a predictable life of 25 cycles for each rock particle.The pore fractal features of the macropores and micro-meso pores show great differences between the early and late cycles,which verifies the computational statistics analysis of particle deterioration.The numerical results show that the failure patterns are governed by the strain in pre-peak stage and the shear cracks are dominant.The dry-wet cycles reduce the energy transfer efficiency and lead to the discretization of force chain and crack fields. 展开更多
关键词 Underground pumped storage power plant dry-wet cycles Split Hopkinson pressure bar Macro and micro properties FEM-DEM coupling model Damage characterization
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A Neural-network-based Alternative Scheme to Include Nonhydrostatic Processes in an Atmospheric Dynamical Core
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作者 Yang XIA Bin WANG +13 位作者 Lijuan LI Li LIU Jianghao LI Li DONG Shiming XU Yiyuan LI Wenwen XIA Wenyu HUANG Juanjuan LIU Yong WANG Hongbo LIU Ye PU Yujun HE Kun XIA 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第6期1083-1099,I0002,I0003,共19页
Here,a nonhydrostatic alternative scheme(NAS)is proposed for the grey zone where the nonhydrostatic impact on the atmosphere is evident but not large enough to justify the necessity to include an implicit nonhydrostat... Here,a nonhydrostatic alternative scheme(NAS)is proposed for the grey zone where the nonhydrostatic impact on the atmosphere is evident but not large enough to justify the necessity to include an implicit nonhydrostatic solver in an atmospheric dynamical core.The NAS is designed to replace this solver,which can be incorporated into any hydrostatic models so that existing well-developed hydrostatic models can effectively serve for a longer time.Recent advances in machine learning(ML)provide a potential tool for capturing the main complicated nonlinear-nonhydrostatic relationship.In this study,an ML approach called a neural network(NN)was adopted to select leading input features and develop the NAS.The NNs were trained and evaluated with 12-day simulation results of dry baroclinic-wave tests by the Weather Research and Forecasting(WRF)model.The forward time difference of the nonhydrostatic tendency was used as the target variable,and the five selected features were the nonhydrostatic tendency at the last time step,and four hydrostatic variables at the current step including geopotential height,pressure in two different forms,and potential temperature,respectively.Finally,a practical NAS was developed with these features and trained layer by layer at a 20-km horizontal resolution,which can accurately reproduce the temporal variation and vertical distribution of the nonhydrostatic tendency.Corrected by the NN-based NAS,the improved hydrostatic solver at different horizontal resolutions can run stably for at least one month and effectively reduce most of the nonhydrostatic errors in terms of system bias,anomaly root-mean-square error,and the error of the wave spatial pattern,which proves the feasibility and superiority of this scheme. 展开更多
关键词 neural network nonhydrostatic alternative scheme atmospheric model dynamical core
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Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing
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作者 Yinqing Yang Lei Zhang +7 位作者 Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin Hou Feng Cheng 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期961-970,共10页
Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,ha... Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,has been widely used as a common reference in biological research.Although its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 transcripts.Additionally,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold stress.Meanwhile,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS analysis.Overall,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms. 展开更多
关键词 Brassica oleracea Oxford nanopore technologies Gene annotation alternative splicing
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2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-Glucoside modulates CHEK2 and CCND1 alternative splicing to inhibit MCF-7 cells proliferation
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作者 Hui Shen You-Zhi Zhang +5 位作者 Peng-Yu Wang Shuo Zhang Huan Pan Bei-Bei Liu Long-Sheng Xu Jian-Fen Shen 《Traditional Medicine Research》 2024年第1期33-46,共14页
Background:In our previous study,we observed a synergistic effect of 2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-glucoside combined with adriamycin to induce apoptosis in MCF-7 breast cancer cells.However,the underlying me... Background:In our previous study,we observed a synergistic effect of 2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-glucoside combined with adriamycin to induce apoptosis in MCF-7 breast cancer cells.However,the underlying mechanisms of epigenetic modifications,such as alternative splicing,have not been explored.In this study,we aimed to investigate the mechanism by which THSG inhibits MCF-7 cell proliferation using full-length transcriptome sequencing.Methods:First,cell viability was examined using the methyl thiazolyl tetrazolium method and full-length transcriptome sequencing was performed to identify genes and pathways.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were used to identify the principal pathways and targets of THSG.Flow cytometry analysis of cell cycle distribution was performed.Meanwhile,the analysis of alternative splicing and domains of the key proteins was conducted.Quantitative polymerase chain reaction and western blotting were performed for verification.Results:THSG showed significant cytotoxic activity in MCF-7 cells.Full-length transcriptome sequencing revealed differential alternative splicing with 173 upregulated and 263 downregulated genes.Further analysis identified distinct differential expression of genes(CHEK2-211 and CCND1-201)involved in the cell cycle in the THSG-treated group.Subsequently,alternative splicing types of CHEK2(mutually exclusive exon)and CCND1(intron retention).We found that THSG downregulated mRNA expression,as confirmed by quantitative polymerase chain reaction analysis.Interestingly,protein structural analysis revealed that THSG treatment led to the generation of CHK2-211,which was the result of a mutation in the amino acid residues(GLU-150,ASN-151)of the CHEK2 domain(VAL-150,GLY-151).and CyclinD1-201 were obtained when an amino acid(ASP-267)in the domain was lost in CyclinD1.Moreover,molecular docking analysis demonstrated that the domains of key proteins could bind THSG more effectively,with no difference in affinity.Western blotting confirmed that THSG inhibited the expression of CHK2 and CyclinD1.Conclusion:THSG modulated the alternative splicing of CHEK2 and CCND1 by inducing G0/G1 cell cycle arrest,consequently suppressing MCF-7 cell proliferation. 展开更多
关键词 THSG breast cancer full-length transcriptome sequencing alternative splicing
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Workshop “Navigating the Transition: Standards Powering the Journey of Alternative Fuel Infrastructure”
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《China Standardization》 2024年第2期67-67,共1页
April 18, Brussels, Belgium In light of recent significant regulatory changes within the European Union impacting the transport sector, such as the Alternative Fuels Infrastructure Regulation(AFIR), Re Fuel EU and Fue... April 18, Brussels, Belgium In light of recent significant regulatory changes within the European Union impacting the transport sector, such as the Alternative Fuels Infrastructure Regulation(AFIR), Re Fuel EU and Fuel EU Maritime, the role of European standardization becomes increasingly critical in facilitating the transition towards the deployment of alternative fuels. 展开更多
关键词 TRANSITION alternATIVE MARITIME
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Use of Topical Complementary and Alternative Medicines (CAM) in Diabetic Septic Foot Disease in Makkah Region, Western of Saudi Arabia, an Exploratory Study
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作者 Mohamed Elamin Salih Hatim Alrizqi +5 位作者 Mohammed Hussain Abdullah Alshumrani Ismail Alhasani Ali Alkhamisi Hamzah Alsayed Fahad Alqarni 《Journal of Biosciences and Medicines》 2024年第3期245-258,共14页
Diabetes is a chronic condition that is significantly impacted daily by environment. At least one in five Saudis, suffer from diabetes. “Diabetes is a pandemic of unprecedented magnitude spiraling out of control” (B... Diabetes is a chronic condition that is significantly impacted daily by environment. At least one in five Saudis, suffer from diabetes. “Diabetes is a pandemic of unprecedented magnitude spiraling out of control” (Boulton). Saudi Arabia ranks the second highest in the Middle East, and is the seventh in the world for the prevalence of diabetes (WHO). Diabetes mellitus (DM) has been found to be related to high mortality, morbidity accompanied by poor general health and lower quality of life. Current Saudi diabetes patients’ behaviors, practices, and beliefs regarding foot health problems such as ulcers, are not well investigated. Diabetic patients frequently utilize natural remedies and Herbs for self-medication as a part of complementary and alternative Medicine. Aim of study: To explore the widespread use of the alternative remedies in the local communities of the Western Saudi Arabia, the drives behind using and to focus on the potential adverse effects following their usage. Method: A descriptive, cross-sectional study of 386 diabetic patients with feet problems in Makkah Region, West of Saudi Arabia during the period April - June 2023. Results: Out of the 531 participants, only 386 participants reported diabetic foot disorder (DFD). Most of the participant were above 65 years of age group (24.61 %). Male (52.08%) more than female (47.92%) in the research group. All of the participants had diabetic foot disorders at least once. The prevalence of complementary and alternative medicines (CAM) use among them was 67.88%, as solitary or in combination with hospital medicines. Honey headed the list followed by myrrh and black seeds. Acquaintances advice (59.54%) was the most reason for using the alternative medicine. 60.30% of Diabetic foot patient complained of adverse effects during CAM remedies use. Conclusion: Consumption of CAM remedies among diabetic patients for Diabetic Foot Disorder (DFD) is common. Honey headed the list followed by myrrh and black seeds. Additional wide-scale research is required to establish their actual efficacy, safety and potential adverse out-comes and to generalize the outcomes. 展开更多
关键词 Diabetic Foot Ulcer Diabetes Complementary and alternative Medicine CAM West of Saudi Arabia
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Molecular targets and mechanisms of different aberrant alternative splicing in metastatic liver cancer
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作者 De-Yi Geng Qing-Shan Chen +7 位作者 Wan-Xian Chen Lin-Sa Zhou Xiao-Sha Han Qi-Hu Xie Geng-Hong Guo Xue-Fen Chen Jia-Sheng Chen Xiao-Ping Zhong 《World Journal of Clinical Oncology》 2024年第4期531-539,共9页
Metastasis remains a major challenge in the successful management of malignant diseases.The liver is a major site of metastatic disease and a leading cause of death from gastrointestinal malignancies such as colon,sto... Metastasis remains a major challenge in the successful management of malignant diseases.The liver is a major site of metastatic disease and a leading cause of death from gastrointestinal malignancies such as colon,stomach,and pancreatic cancers,as well as melanoma,breast cancer,and sarcoma.As an important factor that influences the development of metastatic liver cancer,alternative splicing drives the diversity of RNA transcripts and protein subtypes,which may provide potential to broaden the target space.In particular,the dysfunction of splicing factors and abnormal expression of splicing variants are associated with the occurrence,progression,aggressiveness,and drug resistance of cancers caused by the selective splicing of specific genes.This review is the first to provide a detailed summary of the normal splicing process and alterations that occur during metastatic liver cancer.It will cover the role of alternative splicing in the mechanisms of metastatic liver cancer by examining splicing factor changes,abnormal splicing,and the contribution of hypoxia to these changes during metastasis. 展开更多
关键词 alternative splicing CARCINOMA HEPATOCELLULAR Metastasic Liver neoplasms PROGNOSIS
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