This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied amo...This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied among different inbred lines. To be specific, chlorophyll content of No. 68 inbred line was significantly higher than that of other 14 inbred lines ; chlorophyll content of No. 55 inbred line was signifieandy lower than that of other 15 inbred lines. The chlorophyll content of various inbred lines showed a decreasing order of No. 68 〉 No. 47 〉 No. 66 〉 No. 62 〉 No. 63 〉 No. 60 〉 No. 61 〉 No. 64 〉 No. 56 〉 No. 67 〉 No. 65 〉 No. 48 〉 No. 59 〉 No. 58 〉 No. 57 〉 No. 69 〉 No. 55 ; the photosynthetic rate of various inbred lines showed a decreasing order of No. 68 〉 No. 57 〉 No. 60 〉 No. 48 〉 No. 58 〉 No. 47 〉 No. 62 〉 No. 63 〉 No. 64 〉 No. 65 ; the maximum photesynthetie rate of No. 65 inbred line was significantly lower than other seven inbred lines, while no signifi- cant difference was observed among other nine inbred lines.展开更多
The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situat...The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.展开更多
The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temper...The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.展开更多
An appropriate genetic similarity coefficient is particularly important for accurately estimating the genetic similarity and phylogenetic relationship between individuals and evaluating the genetic diversity of popula...An appropriate genetic similarity coefficient is particularly important for accurately estimating the genetic similarity and phylogenetic relationship between individuals and evaluating the genetic diversity of populations. In this study, five genetic similarity coefficients were compared for analysis of phylogenetic relationship among 31 hot pepper inbred lines based on SRAP. The applicability of different genetic similarity coefficient was investigated by means of SRAP data of hot pepper inbred lines. According to the experimental results, the variation ranges of Nei ＆ Li, Jaceard, Sorensen, Simple matching and Yule coefficients were 0. 598 - 0. 973, 0. 427 - 0. 947, 0. 598 - 0. 973, 0.427 - 0. 947 and 0. 133 - 0. 997, respectively. Results of cluster analysis based on different similarity coefficients varied greatly. To be specific, clustering results based on Nei ＆ Li, Jaccard and Sorensen coefficients were consistent; clustering with Simple matching and Yule coef ficients led to consistent classification of category in different order and slightly different classification of subcategory. Comprehensively comparing the results of cluster analysis and the dendrograms of hot pepper inbred lines, Yule coefficient is suitable for SRAP analysis of hot pepper.展开更多
基金Supported by vegetable Station of Ningxia Hui Autonomous Region
文摘This study aimed to investigate chlorophyll content and photosynthetic characteristics of 17 pepper inbred lines which were selected independently. The results showed that chlorophyll contents significantly varied among different inbred lines. To be specific, chlorophyll content of No. 68 inbred line was significantly higher than that of other 14 inbred lines ; chlorophyll content of No. 55 inbred line was signifieandy lower than that of other 15 inbred lines. The chlorophyll content of various inbred lines showed a decreasing order of No. 68 〉 No. 47 〉 No. 66 〉 No. 62 〉 No. 63 〉 No. 60 〉 No. 61 〉 No. 64 〉 No. 56 〉 No. 67 〉 No. 65 〉 No. 48 〉 No. 59 〉 No. 58 〉 No. 57 〉 No. 69 〉 No. 55 ; the photosynthetic rate of various inbred lines showed a decreasing order of No. 68 〉 No. 57 〉 No. 60 〉 No. 48 〉 No. 58 〉 No. 47 〉 No. 62 〉 No. 63 〉 No. 64 〉 No. 65 ; the maximum photesynthetie rate of No. 65 inbred line was significantly lower than other seven inbred lines, while no signifi- cant difference was observed among other nine inbred lines.
基金Supported by the Project for the Commercialization of Agricultural Research Findings in China(2011GB2D200007)the National High Technology Research and Development Program of China (863 Program) Planning for the Development of High-Technology Research in China(2010AA101304)the National Natural Science Foundation of China(31371714)~~
文摘The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.
基金supported by the National High Technology Research and Development Program of (Grant No.2010AA101304)the Transformation Fund for Agricultural Science and Technology Achievements (Grant No.2007GB2D200226)the Natural Science Foundation of Hunan Province,China(Grant No. 10JJ4012)
文摘The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.
基金Supported by Natural Science Foundation of Hainan Province"Construction of Genetic Linkage Map of Dendrobium"(312024)China Spark Program"Pilotscale Trial and Demonstration of New Varieties of Tropical Flowers"(2012GA800003)Special Fund for Basic Scientific Research of Central Nonprofit Research Institutes"Study on the Cold Stress Response Mechanism and Breeding of Cold-resistant in Dendrobium phalaenopsis"(1630032014017)
文摘An appropriate genetic similarity coefficient is particularly important for accurately estimating the genetic similarity and phylogenetic relationship between individuals and evaluating the genetic diversity of populations. In this study, five genetic similarity coefficients were compared for analysis of phylogenetic relationship among 31 hot pepper inbred lines based on SRAP. The applicability of different genetic similarity coefficient was investigated by means of SRAP data of hot pepper inbred lines. According to the experimental results, the variation ranges of Nei ＆ Li, Jaceard, Sorensen, Simple matching and Yule coefficients were 0. 598 - 0. 973, 0. 427 - 0. 947, 0. 598 - 0. 973, 0.427 - 0. 947 and 0. 133 - 0. 997, respectively. Results of cluster analysis based on different similarity coefficients varied greatly. To be specific, clustering results based on Nei ＆ Li, Jaccard and Sorensen coefficients were consistent; clustering with Simple matching and Yule coef ficients led to consistent classification of category in different order and slightly different classification of subcategory. Comprehensively comparing the results of cluster analysis and the dendrograms of hot pepper inbred lines, Yule coefficient is suitable for SRAP analysis of hot pepper.
