期刊文献+
共找到4,771篇文章
< 1 2 239 >
每页显示 20 50 100
Progress in weldability research of duplex stainless steels
1
作者 刘爱国 《China Welding》 CAS 2024年第2期50-62,共13页
Duplex stainless steels(DSSs)show better corrosion resistance with higher strength than traditional austenite stainless steels in many aggressive environments,and can be welded properly with almost every welding proce... Duplex stainless steels(DSSs)show better corrosion resistance with higher strength than traditional austenite stainless steels in many aggressive environments,and can be welded properly with almost every welding processes,if proper heat input is provided.Progresses of research works on weldability of DSSs in recent years are reviewed in this paper.Balance control of ferrite/austenite phases is most important for DSSs welding.The phases balance can be controlled with filler materials,nitrogen addition in shielding gas,heat input,post weld heat treatment,and alternating magnetic field.Too high cooling rate results in not only extra ferrite,but also chromium nitride precipitation.While too low cooling rate or heating repeatedly results in precipitation of secondary austenite and intermetallic compounds.In both situations,mechanical properties and corrosion resistance of the DSS joints deteriorate.Recommended upper and lower limits of heat input and maximum interpass temperature should be observed. 展开更多
关键词 duplex stainless steel WELDABILITY phases balance secondary phase
下载PDF
基于CP基因的云南烟草花叶病毒RT-LAMP快速检测体系的构建
2
作者 赵正婷 盖晓彤 +3 位作者 张俊蕾 卢灿华 姜宁 刘雅婷 《山东农业科学》 北大核心 2024年第5期154-162,共9页
为快速检测云南烟草花叶病毒(Tobacco mosaic virus,TMV),本研究根据来自云南烟草TMV分离物的外壳蛋白(CP)基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对反应的温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度及内、外引... 为快速检测云南烟草花叶病毒(Tobacco mosaic virus,TMV),本研究根据来自云南烟草TMV分离物的外壳蛋白(CP)基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对反应的温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度及内、外引物浓度比等进行逐一优化,建立了云南烟草TMV逆转录环介导等温扩增(RT-LAMP)检测体系。结果表明,最佳引物组为第4组,最适反应温度为60℃,甜菜碱、dNTPs、Mg^(2+)的最佳反应浓度分别为0.6、0.4、2.0 mmol/L,最佳内、外引物浓度比为4∶1,最佳反应时间40 min。优化后的RT-LAMP经SYBR Green I染色可肉眼判断结果,特异性高,灵敏度是常规RT-PCR的10倍。RT-LAMP检测体系的建立为云南烟草TMV的检测提供了一种便捷、高效、可靠的方法。 展开更多
关键词 烟草花叶病毒 CP基因 rt-lamp 特异性 灵敏度
下载PDF
Innovative COVID-19 Screening: RT-LAMP Assay for Spike and NSP1 Proteins
3
作者 Muhammad Yousaf Amber Khan 《Journal of Clinical and Nursing Research》 2024年第6期256-265,共10页
Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus globally.It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and... Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus globally.It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and detection.Owing to this situation,RT-LAMP appears to be a novel method for the identification of COVID-19 because of its vast applications,including cost-effectiveness and time-saving.This research highlights the use of RT-LAMP,a more sensitive test than RT-PCR,for the assessment of SARS-CoV-2,the severe acute respiratory illness.To identify the spike(S)and NSP1 protein using RT-LAMP,170 total samples of coronavirus-suspected patients were served in this research.Health certifications and bioethical considerations were taken into consideration.After the sample was extracted from the patient's swabs,RNA was isolated,extracted,and purified.The response was then run on the RT-LAMP at the ideal temperature,and the outcomes could be observed with the unaided eye as they changed from pink to yellow.It is a simple method of determining if the test is positive or negative.For this purpose,both RT-LAMP and RT-PCR tests are used during these procedures.Genes linked with COVID-19 testing including S,nspl,and ORF are suited to coronavirus testing;they have 100%specificity and low sensitivity,but S has more specificity and sensitivity than nspl and ORF,respectively.Out of the 95 positive samples,89(93.68%)samples yielded favorable outcomes utilizing RT-LAMP,while 55 negative samples yielded 100%positive results.The present research demonstrates that RT-LAMP is less sensitive yet more selective for coronavirus detection. 展开更多
关键词 COVID-19 NSP1 SPIKE rt-lamp
下载PDF
二重荧光RT-LAMP鉴别检测禽呼肠孤病毒和鸡滑液囊支原体
4
作者 曾婷婷 谢芝勋 +9 位作者 谢丽基 王盛 谢志勤 黄娇玲 万丽军 任红玉 张艳芳 张民秀 范晴 邓显文 《畜牧与兽医》 CAS 北大核心 2023年第9期66-75,共10页
本研究旨在建立一套通过添加荧光探针FD,反应后可使用成像仪直接观测结果的鉴别检测禽呼肠孤病毒(ARV)和鸡滑液囊支原体(MS)的二重荧光RT-LAMP检测方法。根据ARV和MS的保守基因片段,设计了2套LAMP引物,并各添加1条FD荧光探针,2条FD探针... 本研究旨在建立一套通过添加荧光探针FD,反应后可使用成像仪直接观测结果的鉴别检测禽呼肠孤病毒(ARV)和鸡滑液囊支原体(MS)的二重荧光RT-LAMP检测方法。根据ARV和MS的保守基因片段,设计了2套LAMP引物,并各添加1条FD荧光探针,2条FD探针分别标记CY5和6-FAM荧光基团。结果:ARV阳性对照在成像仪下发出红色荧光,MS阳性对照发出绿色荧光,双重模板经过图像融合后为黄色荧光;特异性结果显示,该二重荧光RT-LAMP检测方法仅能扩增ARV和MS两种病原,对其他常见病原如鸡毒支原体(MG)、禽流感病毒(AIV)、新城疫病毒(NDV)等无扩增反应;敏感性结果显示,该二重荧光RT-LAMP检测方法对ARV和MS的最低检测量分别为1.7×10^(2)copies和1.9×10^(2)copies;使用该方法检测40份临床样品,检出率与本实验室前期建立的二重荧光RT-PCR检测结果相同。综上,该二重荧光RT-LAMP检测方法可特异、敏感地鉴别检测ARV和MS,通过添加FD荧光探针和成像仪观测结果,为不同条件的实验室提供了新的检测手段。 展开更多
关键词 FD荧光探针 二重荧光rt-lamp 禽呼肠孤病毒 鸡滑液囊支原体
下载PDF
辣椒轻斑驳病毒RT-LAMP快速检测方法的建立 被引量:1
5
作者 邵煜尊 张万红 +6 位作者 王惠 王玉洁 苗圃 申莉莉 李莹 焦裕冰 杨金广 《中国蔬菜》 北大核心 2023年第12期42-48,共7页
辣椒轻斑驳病毒(pepper mild mottle virus,PMMo V)是发生在辣椒上的一种重要病毒病。通过逆转录环介导等温扩增技术(RT-LAMP),以PMMo V外壳蛋白CP序列为靶标基因,设计F3/B3、FIP/BIP 4条引物,构建了基于RT-LAMP技术的PMMo V快速检测方... 辣椒轻斑驳病毒(pepper mild mottle virus,PMMo V)是发生在辣椒上的一种重要病毒病。通过逆转录环介导等温扩增技术(RT-LAMP),以PMMo V外壳蛋白CP序列为靶标基因,设计F3/B3、FIP/BIP 4条引物,构建了基于RT-LAMP技术的PMMo V快速检测方法。结果表明,该方法在60 min内便可完成对PMMo V的特异性检测,灵敏度比传统RT-PCR技术高10倍,检测过程中与其他相关病毒无交叉反应。本试验建立的PMMo V RT-LAMP检测方法操作简便、特异性强、灵敏度高,可用于田间生产中对PMMo V的快速检测。 展开更多
关键词 辣椒轻斑驳病毒 rt-lamp检测 快速检测
下载PDF
猪流行性腹泻病毒可视化RT-LAMP检测方法的建立与初步应用 被引量:6
6
作者 李林岳 李任峰 +6 位作者 袁嘉康 秦保亮 庞俊增 蔡琳琳 赵晓会 范国英 王自良 《中国预防兽医学报》 CAS CSCD 北大核心 2023年第8期814-821,828,共9页
为建立猪流行性腹泻病毒(PEDV)可视化反转录-环介导等温扩增(RT-LAMP)检测方法,本研究根据PEDV ORF1a/b基因的保守序列,设计了8组特异性引物,以PEDC cDNA为模板,采用试剂盒推荐的各反应条件经LAMP扩增,根据LAMP反应产物琼脂糖凝胶电泳... 为建立猪流行性腹泻病毒(PEDV)可视化反转录-环介导等温扩增(RT-LAMP)检测方法,本研究根据PEDV ORF1a/b基因的保守序列,设计了8组特异性引物,以PEDC cDNA为模板,采用试剂盒推荐的各反应条件经LAMP扩增,根据LAMP反应产物琼脂糖凝胶电泳结果筛选最佳引物组。以筛选的外引物PE2 F3/B3经PCR从PEDV cDNA中扩增ORF1a/b基因,构建重组质粒pPE-ORF1-2并分别经PCR和测序鉴定。结果显示,2号引物组(内引物PE2 FIP/PE2 BIP,外引物PE2 F3/PE2 B3)为最佳引物。PCR和测序鉴定结果表明重组质粒正确构建,经计算其浓度为1.56×1011拷贝/μL,将其稀释1000倍后作为质粒标准品。为建立检测PEDV的RT-LAMP方法,本研究对该方法的反应时间、反应温度、Mg2+浓度、内、外引物及dNTP浓度进行了优化,结果显示,RT-LAMP反应体系为:60℃反应50 min,Mg2+浓度80 mmol/L,内外引物终浓度分别为32μmol/L和5μmol/L,dNTP浓度10 mmol/L;通过在上述体系中加入甲酚红指示剂,初步建立了可视化RT-LAMP检测方法。特异性试验结果显示,该方法仅能检出PEDV,与猪德尔塔冠状病毒、猪繁殖与呼吸综合征病毒和猪传染性胃肠炎病毒均无交叉反应,且阳性管体系颜色由紫红色变为黄色,阴性反应管体系颜色保持紫红色,特异性较强;将重组质粒标准品pPE-ORF1-210倍倍比稀释(1.65×100拷贝/μL~1.65×108拷贝/μL)后作为模板,采用本研究建立的可视化RT-LAMP方法进行敏感性试验,结果显示,该方法对pPE-ORF1-2检测限为10拷贝/μL,比以重组质粒pPE-M-GB为模板,以引物PEGBF/PEGBR的PEDV国标RT-PCR方法的敏感性高1个数量级,敏感性较高;重复性试验结果显示,该方法对不同浓度质粒标准品的批内和批间重复性试验的变异系数均小于5%,重复性较好。对53份临床样品检测结果显示,RT-LAMP和RT-PCR对PEDV的检出率分别为32.1%(17/53)和28.3%(15/53)。二者的总符合率为96.2%(51/53)。本研究建立的PEDV RT-LAMP检测方法具有特异性强、敏感性高、重复性好、操作简便、结果可视化等优点,为PEDV的现场快速检测提供了新的技术支持。 展开更多
关键词 猪流行性腹泻病毒 rt-lamp 快速检测 可视化
下载PDF
葡萄卷叶伴随病毒-3 RT-LAMP检测体系的建立与应用 被引量:1
7
作者 张强强 王雯雯 +3 位作者 闫思远 李玲 王若彤 顾沛雯 《核农学报》 CAS CSCD 北大核心 2023年第11期2166-2174,共9页
为实现葡萄生产中对葡萄卷叶伴随病毒-3(GLRaV-3)的快速检测。本研究以GLRaV-3的CP基因序列(GenBank登录号:KC477128.1)为靶序列设计并合成了3对逆转录环介导恒温扩增技术(RTLAMP)引物,建立并优化GLRaV-3的RT-LAMP检测方法。结果表明,... 为实现葡萄生产中对葡萄卷叶伴随病毒-3(GLRaV-3)的快速检测。本研究以GLRaV-3的CP基因序列(GenBank登录号:KC477128.1)为靶序列设计并合成了3对逆转录环介导恒温扩增技术(RTLAMP)引物,建立并优化GLRaV-3的RT-LAMP检测方法。结果表明,该方法能够实现在恒温64℃下反应1 h完成对GLRaV-3的检测,引物GLRaV3-LAMP-Ⅰ特异性高,不与GLRaV-1~2、葡萄扇叶病毒、沙地葡萄茎痘相关病毒、葡萄病毒A、灰皮诺葡萄病毒和葡萄浆果内坏死病毒等7种葡萄常见病毒发生交叉反应;RNA最低检测限为10 pg·μL^(-1),灵敏度是逆转录PCR(RT-PCR)的10倍。田间样品检测验证结果表明,该方法与常规RT-PCR法检测一致率为100%。综上,本研究建立的GLRaV-3 RT-LAMP检测体系具有高特异性、高灵敏度和实用性强等特点,可应用于田间葡萄卷叶病样的检测。 展开更多
关键词 葡萄卷叶伴随病毒3 rt-lamp 引物筛选 反应条件优化 田间检测
下载PDF
Developing a duplex ARMS-qPCR method to differentiate genotype Ⅰ and Ⅱ African swine fever viruses based on their B646L genes 被引量:2
8
作者 DING Lei-lei REN Tao +6 位作者 HUANG Lian-yu Weldu TESFAGABER ZHU Yuan-mao LI Fang SUN En-cheng BU Zhi-gao ZHAO Dong-ming 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第5期1603-1607,共5页
Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for ... Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for Animal Health (WOAH)(Wang N et al. 2019). 展开更多
关键词 SWINE FEVER duplex
下载PDF
朱顶红褪绿环斑病毒RT-LAMP快速检测体系的建立
9
作者 赵正婷 盖晓彤 +3 位作者 张俊蕾 夏振远 姜宁 刘雅婷 《中国烟草科学》 CSCD 北大核心 2023年第3期39-46,共8页
为快速检测朱顶红褪绿环斑病毒(hippeastrum chlorotic ringspot virus, HCRV),根据HCRV的核壳体(N)蛋白基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对RT-LAMP体系反应温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度、... 为快速检测朱顶红褪绿环斑病毒(hippeastrum chlorotic ringspot virus, HCRV),根据HCRV的核壳体(N)蛋白基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对RT-LAMP体系反应温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度、内外引物浓度比等进行逐一优化,建立了HCRV逆转录环介导等温扩增(RT-LAMP)检测体系。结果表明,最佳引物组为P1,最适反应温度为64℃,Betaine、dNTPs、Mg2+的最佳终浓度分别为0.8、0.2、6 mmol/L,最佳内外引物浓度比为5∶1,最佳反应时间50 min。检测结果显示优化后的RT-LAMP经SYBR Green I染色可通过肉眼直接判断结果,具有高度特异性,且灵敏度是常规RT-PCR的100倍。本研究为HCRV的检测提供了一种便捷、高效、可靠的方法。 展开更多
关键词 朱顶红褪绿环斑病毒 rt-lamp 特异性 灵敏度
下载PDF
Joint Flexible Duplexing and Power Allocation with Deep Reinforcement Learning in Cell-Free Massive MIMO System 被引量:4
10
作者 Danhao Deng Chaowei Wang +2 位作者 Zhi Zhang Lihua Li Weidong Wang 《China Communications》 SCIE CSCD 2023年第4期73-85,共13页
Network-assisted full duplex(NAFD)cellfree(CF)massive MIMO has drawn increasing attention in 6G evolvement.In this paper,we build an NAFD CF system in which the users and access points(APs)can flexibly select their du... Network-assisted full duplex(NAFD)cellfree(CF)massive MIMO has drawn increasing attention in 6G evolvement.In this paper,we build an NAFD CF system in which the users and access points(APs)can flexibly select their duplex modes to increase the link spectral efficiency.Then we formulate a joint flexible duplexing and power allocation problem to balance the user fairness and system spectral efficiency.We further transform the problem into a probability optimization to accommodate the shortterm communications.In contrast with the instant performance optimization,the probability optimization belongs to a sequential decision making problem,and thus we reformulate it as a Markov Decision Process(MDP).We utilizes deep reinforcement learning(DRL)algorithm to search the solution from a large state-action space,and propose an asynchronous advantage actor-critic(A3C)-based scheme to reduce the chance of converging to the suboptimal policy.Simulation results demonstrate that the A3C-based scheme is superior to the baseline schemes in term of the complexity,accumulated log spectral efficiency,and stability. 展开更多
关键词 cell-free massive MIMO flexible duplexing sum fair spectral efficiency deep reinforcement learning asynchronous advantage actor-critic
下载PDF
Investigation on influence of alloying on phase transitions of duplex stainless steel based on thermochemical calculation
11
作者 张志强 刘博 +3 位作者 徐连勇 韩永典 赵雷 曲思成 《China Welding》 CAS 2023年第4期11-28,共18页
This paper investigated on influence of different alloying elements added into duplex stainless steel (DSS) on phase transitions using thermochemical methods in comparison with experiment.The results showed that the m... This paper investigated on influence of different alloying elements added into duplex stainless steel (DSS) on phase transitions using thermochemical methods in comparison with experiment.The results showed that the most possible species in the ferrite phase,austenite phase,σphase,Hcp phase,χphase,and carbide were Cr:Va-type,Fe:Va-type,Ni:Cr:Mo-type,Cr_(2)N-type,Fe_(24)Mo_(10)Cr_(24)-type,and Cr:Mo:C-type,respectively.Furthermore,the Ni,N,Cr,and Mo alloying had significant influences on the transition of each DSS phase.The Ni and N additions obviously raised the temperature at ferrite-1/austenite-1 balance while the Cr and Mo decreased the dual-phase balance temperature.In addition,the Ni addition can promote the precipitating ofσphase at relatively high temperature while the precipitating of Hcp phase at relatively low temperature.The Hcp phase andχphase can be obviously increased by the N addition.The introduction of Cr and Mo notably enhances the precipitation ofσphase.However,the promotion ofχphase precipitation is facilitated by the presence of Mo,while the Cr element acts as an inhibitor forχphase precipitation.Furthermore,the ferrite/austenite ratio tested by experiment was higher than that calculated by thermochemical methods,thus pre-designed solution temperature should be lower about 30-100℃than that calculated by thermochemical methods. 展开更多
关键词 phase transitions PRECIPITATION ALLOYING THERMOCHEMISTRY duplex stainless steel
下载PDF
High throughput screening of localised and general corrosion in type 2205 duplex stainless steel at ambient temperature
12
作者 Yiqi Zhou Sultan Mahmood Dirk Lars Engelberg 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第12期2375-2385,共11页
Bipolar electrochemistry is used to produce a linear potential gradient across a bipolar electrode(BPE),providing direct access to the anodic and cathodic reactions under a wide range of applied potentials.The occurre... Bipolar electrochemistry is used to produce a linear potential gradient across a bipolar electrode(BPE),providing direct access to the anodic and cathodic reactions under a wide range of applied potentials.The occurrence of pitting corrosion,crevice corrosion,and general corrosion on type 2205 duplex stainless steel(DSS 2205)BPE has been observed at room temperature.The critical pit depth of 10-20μm with a55%-75% probability of pits developing into stable pits at potential from+0.9 to+1.2 V vs.OCP(open circuit potential)are measured.All pit nucleation sites are either within ferritic grains or at the interface between austenite and ferrite.The critical conditions for pitting and crevice corrosion are discussed with Epit(critical pitting potential)and Ecre(critical crevice potential)decreasing from 0.87 and 0.80 V vs.OCP after150 s of exposure to 0.84 and 0.76 V vs.OCP after 900 s of exposure,respectively.Pit growth kinetics under different applied bipolar potentials and exposure times have been obtained.The ferrite is shown to be more susceptible to general dissolution. 展开更多
关键词 bipolar electrochemistry duplex stainless steel pitting corrosion pit growth factor crevice corrosion
下载PDF
Literature Review of Phase Transformations and Cavitation Erosion of Duplex Stainless Steels
13
作者 Wenji Ai Shanshui Zheng +1 位作者 Xianfeng Zeng Huibing Cheng 《Journal of Materials Science and Chemical Engineering》 2023年第12期10-21,共12页
Phase transformation is one of the factors that would significantly influence the ability to resist cavitation erosion of stainless steels. Due to the specific properties of duplex stainless steel, the heat treatment ... Phase transformation is one of the factors that would significantly influence the ability to resist cavitation erosion of stainless steels. Due to the specific properties of duplex stainless steel, the heat treatment would bring about significant phase transformations. In this paper, we have examined the previous studies on the phase transition of stainless steel, including the literature on the classification of stainless steel, spinodal decomposition, sigma phase transformation, and cavitation erosion of double stainless steel. Through these literature investigations, the destruction of cavitation erosion on duplex stainless steel can be clearly known, and the causes of failure of duplex stainless steel in seawater can be clarified, thus providing a theoretical basis for subsequent scientific research. And the review is about to help assess the possibility of using bulk heat treatment to improve the cavitation erosion (CE) behaviour of the duplex stainless steel 7MoPLUS. 展开更多
关键词 duplex Stainless Steel Phase Transformation Cavitaion Erosion
下载PDF
Deformation Mechanism of Bimodal Structured 2205 Duplex Stainless Steel in Two Yield Stages
14
作者 盛捷 DU Mingchen +7 位作者 LI Yufeng MA Guocai CHEN Weiqian ZHENG Yuehong ZHAN Faqi REN Junqiang G I Raab 喇培清 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2023年第1期184-191,共8页
A kind of micro/nanostructured 2205 duplex stainless steel(DSS)with uniform distribution of nanocrystals was prepared via aluminothermic reaction method.The analysis of stress-strain curve showed that the fracture str... A kind of micro/nanostructured 2205 duplex stainless steel(DSS)with uniform distribution of nanocrystals was prepared via aluminothermic reaction method.The analysis of stress-strain curve showed that the fracture strength and elongation of the specimen were 946 MPa and 24.7%,respectively.At present,the research on microstructure of bimodal 2205 DSS at room temperature(RT)mainly depended on scanning electron microscope(SEM)observation after loading experiments.The test result indicates that there are two different yield stages in stress-strain curve of specimen during tensile process.The microstructure of duplex bimodal structured stainless steel consists of two pairs of soft hard regions and phases.By studying deformation mechanism of bimodal structured stainless steel,the interaction between soft phase and hard phase are discussed.The principle of composition design and microstructure control of typical duplex stainless steel is obtained,which provides an important research basis for designing of advanced duplex stainless steel. 展开更多
关键词 2205 duplex stainless steel bimodal structure in-situ tensile test deformation mechanism two different yield stages
下载PDF
基于免核酸提取可视化RT-LAMP快速检测黄瓜绿斑驳花叶病毒
15
作者 尹新颖 曹际娟 +2 位作者 李鑫 杨莉莉 朴永哲 《植物保护》 CAS CSCD 北大核心 2023年第2期264-271,共8页
本文基于逆转录环介导恒温扩增(RT-LAMP)技术建立葫芦科作物中黄瓜绿斑驳花叶病毒(CGMMV)免核酸提取的可视化快速检测方法。根据CGMMV全基因组序列,筛选保守区域并设计特异性RT-LAMP检测引物。通过荧光扩增方法(加SYTO-9)和可视化方法(... 本文基于逆转录环介导恒温扩增(RT-LAMP)技术建立葫芦科作物中黄瓜绿斑驳花叶病毒(CGMMV)免核酸提取的可视化快速检测方法。根据CGMMV全基因组序列,筛选保守区域并设计特异性RT-LAMP检测引物。通过荧光扩增方法(加SYTO-9)和可视化方法(加钙黄绿素),开展RT-LMAP特异性、灵敏度和重现性试验分析。结果表明,优化筛选的引物组可以特异性地检测CGMMV,检测灵敏度达到4.21×10^(3) fg/μL,组内和组间变异系数均小于5%,重现性好。对瓜类作物田间种苗、植株叶片及市售种子等103份样品进行检测及一致性分析,表明该方法与基于RNA提取RT-LAMP、荧光RT-qPCR及免疫测试条检测结果之间的Kappa值均为1.0(1~1,95%置信区间),具有很好的一致性。基于免核酸提取的可视化RT-LAMP快检方法成为适合现场快速检测的理想选择,对于CGMMV危害葫芦科作物的田间监测以及疫情防控具有广泛的推广应用前景。 展开更多
关键词 黄瓜绿斑驳花叶病毒 逆转录环介导恒温扩增(rt-lamp) 可视化 免核酸提取
下载PDF
In Band Full Duplex (IBFD) Technology for Next Generation Wireless Networks: A Survey in Cellular Networks
16
作者 Injila G.R.Begh 《China Communications》 SCIE CSCD 2023年第5期20-39,共20页
Next-Generation(NextG)wireless communication networks with their widespread applications require high data rates,seamless connectivity and high quality of service(QoS).To cope up with an unprecedented rise of data hun... Next-Generation(NextG)wireless communication networks with their widespread applications require high data rates,seamless connectivity and high quality of service(QoS).To cope up with an unprecedented rise of data hungry applications,users demand more spectral resources imposing a limitation on available wireless spectrum.One of the potential solutions to address the spectrum scarce issue is to incorporate in band full duplex(IBFD)or full duplex(FD)paradigm in next generation networks including 5G new radio(NR).Recently,FD has gained the research interest in cellular networks for its potential to double the wireless link capacity and enhancing spectral efficiency(SE).In half duplex(HD)cellular networks,base stations(BSs)can either perform uplink(UL)or downlink(DL)transmission at a particular time instant leading to reduced throughput levels.Due to the advancement in the self interference reduction(SIR)techniques,full duplex base stations(FD-BSs)can be employed to allow simultaneous UL and DL transmissions at the same time–frequency resources as compared to its HD counterpart.It ideally achieves twice the throughput without any additional complexity at user-equipment(UE).This paper covers a detailed survey on FD cellular networks.A series of SIR approaches,UE-UE mitigation techniques are summarized.Various existing MAC protocols and antenna architectures for FD cellular networks are outlined.An overview of security aspects for FD in cellular networks is also presented.Lastly,various open issues and possible research directions are brought up for FD cellular networks. 展开更多
关键词 cellular network full duplex(FD)communication self interference reduction(SIR) three node full-duplex(TNFD)topology UE-UE interference
下载PDF
热处理对2205双相不锈钢显微组织及性能的影响 被引量:1
17
作者 王良 杨茗凯 +3 位作者 胡议文 周培山 郑华林 王斌 《材料热处理学报》 CAS CSCD 北大核心 2024年第2期120-128,共9页
采用光学显微镜、显微硬度计和电化学工作站等研究了2205双相不锈钢经不同温度保温不同时间后的显微组织及性能,并分析了其经不同工艺热处理后的γ和α两相比例和析出相的变化。结果表明:2205双相不锈钢初始状态下α和γ相含量分别为41... 采用光学显微镜、显微硬度计和电化学工作站等研究了2205双相不锈钢经不同温度保温不同时间后的显微组织及性能,并分析了其经不同工艺热处理后的γ和α两相比例和析出相的变化。结果表明:2205双相不锈钢初始状态下α和γ相含量分别为41.2%和58.8%。保温时间相同时,随着热处理温度的升高,γ相含量减少,σ相含量先增加后减少,硬度增加,腐蚀电位先降低后增加。保温温度相同时,随着保温时间的延长(0.5~2 h),γ相和σ相含量增加,γ相含量增幅为2.43%~3.87%,σ相含量增幅为0.05%~0.08%,晶粒有长大趋势,硬度逐渐降低,腐蚀电位总体呈降低趋势。当加热温度为950和1000℃时,硬度随保温时间增加而降低的幅度较明显。σ相含量及α/γ比例增加是造成2205双相不锈钢耐腐蚀性能下降的主要原因。 展开更多
关键词 2205双相不锈钢 热处理 显微组织 硬度 耐蚀性
下载PDF
空中可重构智能表面辅助的全双工无人机安全通信
18
作者 罗俊松 黄平 +3 位作者 罗欣悦 赖欢 多滨 袁晓军 《电信科学》 北大核心 2024年第7期34-50,共17页
针对传统的可重构智能表面(reconfigurable intelligent surface,RIS,也称智能超表面)在无人机辅助安全通信中存在的灵活性不足和系统安全保障能力不强的问题,以及当前无人机半双工通信系统普遍面临的频谱利用率低和通信效率低的挑战,... 针对传统的可重构智能表面(reconfigurable intelligent surface,RIS,也称智能超表面)在无人机辅助安全通信中存在的灵活性不足和系统安全保障能力不强的问题,以及当前无人机半双工通信系统普遍面临的频谱利用率低和通信效率低的挑战,提出了一种空中可重构智能表面(aerial reconfigurable intelligent sur‐face,ARIS)与全双工无人机的双机协同解决方案,旨在提升面对地面窃听者时的通信安全性和效率。联合优化用户通信调度、传输功率、无人机发送的干扰功率及其飞行轨迹,以及ARIS的相移和轨迹,设计了一个旨在最大化最小平均可达安全速率的优化问题,并提出基于交替优化(alternating optimization,AO)、半定松弛(semi-definite relaxation,SDR)技术和连续凸近似(successive convex approximation,SCA)的迭代算法,以获得高质量的次优解。仿真结果验证了ARIS与全双工无人机结合的双机协作方案在提升通信质量和安全性能方面的有效性,展示了其在复杂安全通信环境下的优越性。 展开更多
关键词 无人机安全通信 空中可重构智能表面 轨迹优化 全双工
下载PDF
IRS辅助的全双工通感一体化波束成形设计
19
作者 刘期烈 王龙 +3 位作者 辛雅楠 方家成 陈前斌 陶宇 《电信科学》 北大核心 2024年第7期63-75,共13页
为了解决传统通感一体化(integrated sensing and communication,ISAC)场景中通信和感知系统收发信号的切换频率差异以及通信易受障碍物阻挡的问题,将全双工(full-duplex,FD)技术和智能反射面(intelligent reflecting surface,IRS)引入I... 为了解决传统通感一体化(integrated sensing and communication,ISAC)场景中通信和感知系统收发信号的切换频率差异以及通信易受障碍物阻挡的问题,将全双工(full-duplex,FD)技术和智能反射面(intelligent reflecting surface,IRS)引入ISAC系统,研究ISAC场景下IRS辅助的FD波束成形设计。基于IRS相移约束、基站接收波束成形约束、基站最大发射功率约束、上行用户最大发射功率约束、雷达感知阈值约束,建立了上下行用户和速率最大化波束成形优化问题。利用交替优化、连续凸逼近、半正定松弛、变量替换、半正定规划将原非凸优化问题转换成凸优化问题进行求解。仿真结果表明,在所考虑的通信性能指标下,所提方案相比对应的半双工和随机IRS方案分别提升了89.02%和70.15%。 展开更多
关键词 智能反射面 通感一体化 全双工 波束成形
下载PDF
鉴别猪圆环病毒2型和3型双重TaqMan MGB探针FQ-PCR检测方法研究
20
作者 赵雪丽 闫若潜 +8 位作者 王华俊 王淑娟 马震原 谢彩华 柴茂 杨海波 王翠 刘影 王东方 《中国动物传染病学报》 CAS 北大核心 2024年第2期165-173,共9页
建立一种快速、特异鉴别检测猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的双重TaqMan MGB探针FQ-PCR方法,本研究以PCV2的Rep蛋白和PCV3的Cap蛋白基因作为靶基因,各设计1对特异性引物和1条TaqMan MGB探针,经优化各反应条件和进行敏感性... 建立一种快速、特异鉴别检测猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的双重TaqMan MGB探针FQ-PCR方法,本研究以PCV2的Rep蛋白和PCV3的Cap蛋白基因作为靶基因,各设计1对特异性引物和1条TaqMan MGB探针,经优化各反应条件和进行敏感性、特异性、重复性和干扰性试验,建立鉴别检测PCV2/PCV3的双重FQ-PCR方法。结果显示:该方法可特异性扩增PCV2、PCV3核酸,与猪伪狂犬病病毒(PRV)等8种病原及阴性对照无交叉反应,特异性较强;对PCV2和PCV3阳性质粒标准品的最低检出限均可达10 copies/μL,敏感性较高;PCV2/PCV3批内/批间重复试验变异系数(CV)值均在3%以下,表明方法稳定性、重复性较好;干扰性试验表明在两种病毒阳性质粒起始模板相差较大时该方法不会影响对其中任一病毒核酸的检出和准确定量。对42份临床疑似PCV感染样品检测结果与PCV2、PCV3基因测序结果符合率100%。本研究建立的双重FQ-PCR方法具有敏感性高达10 copies/μL、特异性强、在同一反应体系中能同时快速鉴别检测PCV2、PCV3等优点,可用于临床PCV2/PCV3感染的快速鉴别检测。 展开更多
关键词 猪圆环病毒2型 rep基因 猪圆环病毒3型 cap基因 双重TaqMan MGB FQ-PCR
下载PDF
上一页 1 2 239 下一页 到第
使用帮助 返回顶部