Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS_(2) (QD-MB @MoS_(2)) fluoresc...Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS_(2) (QD-MB @MoS_(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.展开更多
基金The authors acknowledge the generous financial support from the National Natural Science Foundation of China(61971207,61975070,51902143,61775088)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Key Research and Development Project of Jiangsu Province(BE2018062,BE2019033)+6 种基金Natural Science Foundation of Jiangsu Province(BK20191467)Post-graduate Research&Practice Innovation Program of Jiangsu Province(KYCX20_2229)International S&T Cooperation Program of Jiangsu Province(BZ2019063,BZ2020045,BZ2020030)Natural Science Foundation of the Jiangsu Higher Education Institutes of China(19KJB430018,20KJA430003)Special Project for Technology Innovation of Xuzhou City(KC19250,KC20201,KC20244)Open Project of State Key Laboratory of Advanced Materials and Electronic Components(FHR-JS-202011017)We also thank Dr.Zhiling Yan for his help and support on the collection of blood samples.WC would like to thank the support from Solgro Inc.,Guangxi Jialouyuan Medical Inc.and UT Arlington distinguished award.
文摘Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS_(2) (QD-MB @MoS_(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.
