Human α1(Ⅰ), α2(Ⅰ) and α1(Ⅲ) cDNA probes and RNA dot hybridization were employed to quantitate collagen mRNA changes after adding silica dust into the media of human 2BS fibroblasts. At all dosages used (100, 20...Human α1(Ⅰ), α2(Ⅰ) and α1(Ⅲ) cDNA probes and RNA dot hybridization were employed to quantitate collagen mRNA changes after adding silica dust into the media of human 2BS fibroblasts. At all dosages used (100, 200, 500 and 1000μg), the α1(Ⅰ), α2(Ⅰ)and α1(Ⅲ) mRNA levels increased one day after dusting. At the same dosage of silica (100μg), α1(Ⅲ) mRNA increased earlier than type Ⅰ collagen mRNA did. The type Ⅰ and type Ⅲ collagen mRNA contents in the experimental groups were higher than those in control on days 3, 5, 7 and 9. The effect of ceruloplasmin (Cp) and fibronectin (Fn) on collagen mRNA synthesis was also studied, after adding silica dust, Cp or Fn into the media of human 2BS fibroblast. The results showed that Cp and Fn have stimulating effect on collagen mRNA production. When both Cp and silica dust were added into cell culture media, the collagen mRNA level was increased more than those of adding either Cp or silica dust alone. Similar situations were found for Fn. Cp (or Fn) synergism with silica dust on stimulating transcription of human collagen gene was suggested展开更多
Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and di...Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and disabilities. The aim of this study was to assess the personal exposure of workers to respirable dust (RD) and respirable crystalline silica (RCS). Method: A cross-sectional study was conducted at the Erdenet Copper Ore Mining and included 581 personal samples of RD and 324 samples of RCS, collected in 2018-2020. Results: The study results revealed that the geometric mean (GM) of RD was 0.35 mg/m<sup>3</sup> (95% CI 0.32 - 0.39). The RD concentration in 5.9% (n = 34) of all the samples exceeded the permissible exposure level (PEL) (3 mg/mg<sup>3</sup>). The GM of RCS was 0.012 mg/m<sup>3</sup> (95% CI 0.010 - 0.016). In total, 12.0% (n = 39) of all RCS sample concentrations exceeded the PEL (0.1 mg/m<sup>3</sup>). Conclusion: Steel smelters, blasters, welders, and maintenance laborers were exposed to RD, while crushers, operators, drilling machinists, and maintenance laborers were exposed to RCS. Therefore, regular monitoring of the work environment, risk-based health examinations for dust-exposed workers, efficient support for dust mitigation, reduction measures at the source, and engineering controls are needed.展开更多
目的探讨石英粉尘接尘人员外周静脉血红细胞膜丙二醛(MDA)负荷分类情况的主要影响因素。方法运用整群抽样研究方法,抽取陶瓷企业石英粉尘健康接尘人员179名,采集外周静脉血2 m l,肝素钠抗凝,以硫代巴比妥法检测红细胞膜MDA负荷,计算MDA...目的探讨石英粉尘接尘人员外周静脉血红细胞膜丙二醛(MDA)负荷分类情况的主要影响因素。方法运用整群抽样研究方法,抽取陶瓷企业石英粉尘健康接尘人员179名,采集外周静脉血2 m l,肝素钠抗凝,以硫代巴比妥法检测红细胞膜MDA负荷,计算MDA的平均负荷作为标准参考值;利用调查表对接尘人员的10个可能影响因素进行调查;同时,对厂内各工序点的生产环境噪声及粉尘浓度进行监测;以接尘人员MDA负荷分类(设大于或小于标准参考值的二分变量)为应变量,以被调查的10个可能影响因素为自变量,运用成组资料的非条件Logistic回归分析模型拟合回归曲线,探寻主要影响因素。结果工龄、粉尘浓度、是否经常佩戴防护口罩、饮茶情况及吸烟等5个因素,最终进入回归方程(α=0.05)。结论进入回归方程的5个因素为影响接尘工人红细胞膜MDA负荷的最主要因素。展开更多
文摘Human α1(Ⅰ), α2(Ⅰ) and α1(Ⅲ) cDNA probes and RNA dot hybridization were employed to quantitate collagen mRNA changes after adding silica dust into the media of human 2BS fibroblasts. At all dosages used (100, 200, 500 and 1000μg), the α1(Ⅰ), α2(Ⅰ)and α1(Ⅲ) mRNA levels increased one day after dusting. At the same dosage of silica (100μg), α1(Ⅲ) mRNA increased earlier than type Ⅰ collagen mRNA did. The type Ⅰ and type Ⅲ collagen mRNA contents in the experimental groups were higher than those in control on days 3, 5, 7 and 9. The effect of ceruloplasmin (Cp) and fibronectin (Fn) on collagen mRNA synthesis was also studied, after adding silica dust, Cp or Fn into the media of human 2BS fibroblast. The results showed that Cp and Fn have stimulating effect on collagen mRNA production. When both Cp and silica dust were added into cell culture media, the collagen mRNA level was increased more than those of adding either Cp or silica dust alone. Similar situations were found for Fn. Cp (or Fn) synergism with silica dust on stimulating transcription of human collagen gene was suggested
文摘Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and disabilities. The aim of this study was to assess the personal exposure of workers to respirable dust (RD) and respirable crystalline silica (RCS). Method: A cross-sectional study was conducted at the Erdenet Copper Ore Mining and included 581 personal samples of RD and 324 samples of RCS, collected in 2018-2020. Results: The study results revealed that the geometric mean (GM) of RD was 0.35 mg/m<sup>3</sup> (95% CI 0.32 - 0.39). The RD concentration in 5.9% (n = 34) of all the samples exceeded the permissible exposure level (PEL) (3 mg/mg<sup>3</sup>). The GM of RCS was 0.012 mg/m<sup>3</sup> (95% CI 0.010 - 0.016). In total, 12.0% (n = 39) of all RCS sample concentrations exceeded the PEL (0.1 mg/m<sup>3</sup>). Conclusion: Steel smelters, blasters, welders, and maintenance laborers were exposed to RD, while crushers, operators, drilling machinists, and maintenance laborers were exposed to RCS. Therefore, regular monitoring of the work environment, risk-based health examinations for dust-exposed workers, efficient support for dust mitigation, reduction measures at the source, and engineering controls are needed.
文摘目的探讨石英粉尘接尘人员外周静脉血红细胞膜丙二醛(MDA)负荷分类情况的主要影响因素。方法运用整群抽样研究方法,抽取陶瓷企业石英粉尘健康接尘人员179名,采集外周静脉血2 m l,肝素钠抗凝,以硫代巴比妥法检测红细胞膜MDA负荷,计算MDA的平均负荷作为标准参考值;利用调查表对接尘人员的10个可能影响因素进行调查;同时,对厂内各工序点的生产环境噪声及粉尘浓度进行监测;以接尘人员MDA负荷分类(设大于或小于标准参考值的二分变量)为应变量,以被调查的10个可能影响因素为自变量,运用成组资料的非条件Logistic回归分析模型拟合回归曲线,探寻主要影响因素。结果工龄、粉尘浓度、是否经常佩戴防护口罩、饮茶情况及吸烟等5个因素,最终进入回归方程(α=0.05)。结论进入回归方程的5个因素为影响接尘工人红细胞膜MDA负荷的最主要因素。
