Steady-migration retention characteristics of peptides under gradient elution： application towards a dynamic separation method for minor-adjustments of the retention of peptides in RPLC

Minor-adjustment of the retention of peptides, induced by varying the mobile phase flow-rate(MPF-R), is a new dynamic separation method for simultaneously and rapidly identifying and improving the selectivity of hidden and overlapping peptide peaks. It can also-stabilize the reverse elution order of some pair-peaks under gradient elution in reverse phase liquid chromatography. The retention characteristics of peptides under gradient elution in RPLC was firstly found to be dominated by two variables of the steady region(SR) and migration region(MR). The changes in peptide retention induced by varying the MPF-R can be attributed to changes in the rate of bond breaking of multiple molecular interactions of peptides from the SR and of the mass transfer of peptides from the stationary phase to the mobile phase in the MR. The two dynamic variables were also found to independently depend on the type of peptide. Desirable results were obtained using six standard oligopeptides and a real sample of trypsin-digested lysozyme.It is expected that the quality control of peptide drugs, high dispersion of peptide peaks in peptide mapping and "bottom-up MS"in proteomics will be improved by this method, even enabling peptide purification on a preparative scale in industry.

Numerical simulation of the pulsing air separation field based on CFD

The flow field of pulsing air separation is normally in an unsteady turbulence state.With the application of the basic principles of multiphase turbulent flows,we established the dynamical computational model,which shows a remarkable variation of the unstable pulsing air flow field.CFD(computational fluid dynamics) was used to conduct the numerical simulation of the actual geometric model of the classifier.The inside velocity of the flowing fields was analyzed later.The simulation results indicate that the designed structure of the active pulsing air classifier provided a favorable environment for the separation of the particles with different physical characters by density.We shot the movement behaviors of the typical tracer grains in the active pulsing flow field using a high speed dynamic camera.The displacement and velocity curves of the particles in the continuous impulse periods were then analyzed.The experimental results indicate that the effective separation by density of the particles with the same settling velocity and different ranges of the density and particle size can be achieved in the active pulsing airflow field.The experimental results provide an agreement with the simulation results.

Stage separation of recoverable liquid launch vehicle by using moving pulsating ball analogue for propellant sloshing

In the process of stage separation of recoverable liquid launch vehicles,because of the large amount of residual fuel in the storage tanks,the influence of liquid sloshing on separation safety must be considered.Considering calculation simplicity and operation practicability,the Moving Pulsating Ball Model(MPBM)of large amplitude liquid sloshing is introduced into the calculation of launch vehicle stage separation.Combining the dynamic equation of the model with the energy relationship during"breathing movement",the formula calculating the force of liquid on the rigid body is derived.Compared with the calculations of commercial CFD calculation software,the accuracy of MPBM model is verified.Then,all the external forces and moments are applied to the rigid body of the stages,so that the translational and rotational dynamic equations of the stages are obtained respectively.According to the relative position of the two stages,the geometric shape of the interstage section and the engine of the second stage,the minimum clearance in the separation process can be decided to guarantee that the separation process is safe.

Simulations of the Transient Flow Generated from a Started Flat Plate

Transient operations are commonly founded in fluid machineries such as the starting, stopping, and variations of rotor speeds, etc. Flow generated from a started fiat plate is of fundamental importance. Experiments have been done to observe the flow evolution in current researches. And in order to explore the flow in more detailed scale, some vortex methods with high resolution and other numerical methods were developed to solve various related problems by some researchers. But the promotion of vortex method to engineering application is rare due to its complexity and difficulty in specifying the boundary conditions. In order to build up a method of numerical study for such problems, a simplified model is built up with a flat plate. The development of two-dimensional viscous incompressible flow generated from an impulsively started and uniformly accelerated infinitesimally thin flat plate is simulated numerically. A dynamic mesh（DM） method based on the spring analogue and local remeshing is applied to realize the mesh motion caused by the started plate. Researches show that the mesh quality will decline under large grid shear force during the updating process. To conquer this problem, a region near the plate is separated to guarantee the mesh quality at location of interest which is the innovation of the present paper. All computations at least cover a period during which the plate translates 6 times its length. The simulated instantaneous velocity profiles, flow structures and drag coefficients under several Reynolds numbers （20 ≤ Re ≤ 126） and accelerations （20 m/s2≤ a ≤ 152 m/s2） are presented and compared with existing results in literatures. Comparisons are found to be satisfactory, confirming the validity of the current proposed method（region separated DM）. The proposed DM method is firstly used to study the transient flow generated from a started flat plate and can be used in further study of transient characteristics during transient operations of turbo machineries.

Cyclone separation in a supercritical water circulating fluidized bed reactor for coallbiomass gasification： Structural design and numerical analysis

A new concept of a supercritical water （SCW） circulating fiuidized bed reactor is proposed to produce hydrogen from coal/biomass gasification. The cyclone is a key component of the reactor system, in this paper, cyclones with a single circular inlet （SCI） or a double circular inlet （DCI） were designed to adapt to the supercritical conditions. We evaluated the separation performance of the two cyclones using numerical simulations. A three-dimensional Reynolds stress model was used to simulate the turbulent flow of the fluid, and a stochastic Lagrangian model was used to simulate the particle motion. The flow fields of both cyclones were three-dimensionally unsteady and similar to those of traditional gas-solid cyclones. Secondary circulation phenomena were discovered and their influence on particle separation was estimated. Analyzing the distribution of the turbulence kinetic energy revealed that the most intensive turbulence existed in the zone near the vortex finder while the flow in the central part was relatively stable. The particle concentration distribution was non-uniform because of centrifugal forces. The distribution area can be divided into three parts according to the motion of the particles. In addition, the separation efficiency of both cyclones increased with the inlet SCaN velocity. Because of its perturbance flow, the DCI separator had higher separation efficiency than the SCI separator under comparable simulations. However, this was at the expense of a higher pressure drop across the cyclone.

Numerical simulation of a dense solid particle flow inside a cyclone separator using the hybrid Euler-Lagrange approach

This paper presents a numerical simulation of the flow inside a cyclone separator at high particle loads. The gas and gas–particle flows were analyzed using a commercial computational fluid dynamics code. The turbulence effects inside the separator were modeled using the Reynolds stress model. The two phase gas–solid particles flow was modeled using a hybrid Euler–Lagrange approach, which accounts for the four-way coupling between phases. The simulations were performed for three inlet velocities of the gaseous phase and several cyclone mass particle loadings. Moreover, the influences of several submodel parameters on the calculated results were investigated. The obtained results were compared against experimental data collected at the in-house experimental rig. The cyclone pressure drop evaluated numerically underpredicts the measured values. The possible reason of this discrepancies was disused.