Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedi...Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedicated chambers. Here we showed, using a totally different experimental setup, that radiated EM pulses illuminating the living material through a specialized antenna (without any direct contact) are able to trigger a rapid release of ATP in cultured murine cells that was concomitant with a drop of intracellular AEC. Despite this rapid and strong response, we found that cell viability and clonogenicity were only slightly affected by the EMF exposure.展开更多
Hydrogen sulfide （H2S） is a newly discovered gaseous signaling molecule and involved in ethylene and ABA-induced stomatal closure. As an important factor, extracellular ATP （eATP） was believed to participate in re...Hydrogen sulfide （H2S） is a newly discovered gaseous signaling molecule and involved in ethylene and ABA-induced stomatal closure. As an important factor, extracellular ATP （eATP） was believed to participate in regulation of stomatal closing. However, the mechanism by which eATP mediates HES-regulated stomatal closure remains unclear. Here, we employed Arabidopsis wild-type and mutant lines of ATP-binding cassette transporters （Atmrp4, Atmrp5 and their double mutant Atmrp4/5） to study the function of eATP in H_2S-regulated stomatal movement. Our results indicated that H_2S affected stomatal closing through stimulating guard cell outward K^＋ current. Moreover, we found that HES induced eATP generation by regulating the activity of an ABC transporter. The inhibitor of ABC transporters, glibenclamide （Gli）, could impair H_2S-regulated stomatal closure and reduce H_2S-dependent eATP accumulation in Atmrp4 and Atmrp5 mutants. In addition, the promotion effect of H_2S on outward K^＋ currents was diminished in Atmrp4/5 double mutant. Our data suggested that hydrogen peroxide （H_2O_2） is required for H_2S-induced stomatal closure, and the production of H_2O_2 is regulated by eATP via NADPH oxidase. Based on this work, we conclude that H_2S-induced stomatal closure requires ABC transporter-dependent eATP pro- duction and subsequent NADPH oxidase-dependent H_2O_2 accumulation.展开更多
文摘Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedicated chambers. Here we showed, using a totally different experimental setup, that radiated EM pulses illuminating the living material through a specialized antenna (without any direct contact) are able to trigger a rapid release of ATP in cultured murine cells that was concomitant with a drop of intracellular AEC. Despite this rapid and strong response, we found that cell viability and clonogenicity were only slightly affected by the EMF exposure.
基金supported by the National Natural Science Foundation of China(31170237)the National Key Laboratory Program of China Agricultural University(SKLPPBKF11001)Shandong Taishan Scholar Program
文摘Hydrogen sulfide （H2S） is a newly discovered gaseous signaling molecule and involved in ethylene and ABA-induced stomatal closure. As an important factor, extracellular ATP （eATP） was believed to participate in regulation of stomatal closing. However, the mechanism by which eATP mediates HES-regulated stomatal closure remains unclear. Here, we employed Arabidopsis wild-type and mutant lines of ATP-binding cassette transporters （Atmrp4, Atmrp5 and their double mutant Atmrp4/5） to study the function of eATP in H_2S-regulated stomatal movement. Our results indicated that H_2S affected stomatal closing through stimulating guard cell outward K^＋ current. Moreover, we found that HES induced eATP generation by regulating the activity of an ABC transporter. The inhibitor of ABC transporters, glibenclamide （Gli）, could impair H_2S-regulated stomatal closure and reduce H_2S-dependent eATP accumulation in Atmrp4 and Atmrp5 mutants. In addition, the promotion effect of H_2S on outward K^＋ currents was diminished in Atmrp4/5 double mutant. Our data suggested that hydrogen peroxide （H_2O_2） is required for H_2S-induced stomatal closure, and the production of H_2O_2 is regulated by eATP via NADPH oxidase. Based on this work, we conclude that H_2S-induced stomatal closure requires ABC transporter-dependent eATP pro- duction and subsequent NADPH oxidase-dependent H_2O_2 accumulation.
