Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto ...Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto Culture Corridor of the Silk Road. Daxiang Mountain Giant Buddha Grottoes is the landmark of Gangu County. The giant Buddha statue stands among cliffs 200 meters high above the ground, and it is the second largest stone-base clay-sculpture Buddha in China. After going through thousands of years, the Giant Buddha Grottoes needs effective and scientific protection. This article was originated from the actual project—The Research on the Construction Technology of Wooden-structure Grotto Eaves in the Daxiang Mountain. This article f inished the rebuilding plan for the Buddha Grotto Eaves on the basis of analyzing historical documents, hole traces, morphological evolution and image demonstration.展开更多
[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction sy...[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction system was optimized.Standard curves were established,leading to the initial development of the EAV fluorescence quantitative RT-PCR assay.The accuracy,specificity,and sensitivity of this method were subsequently evaluated.[Result]The EAV fluorescence quantitative RT-PCR assay demonstrated optimal performance at an annealing temperature of 61 C,with a final concentration of primer and probe set at 0.6μmol/L.The plasmid standard demonstrated a strong linear correlation with Ct values within the range of 1.6×10^(7)-1.6×10^(2)copies/μL.The equation of the standard curve was determined to be y=-2.68x+32.88,with an R^(2) value of 0.9927.Consequently,the EAV fluorescence quantitative RT-PCR assay was successfully established.The methodology employed was effective in detecting EAV,Theileria equi,equine herpesvirus-1(EHV-1),equine herpesvirus-4(EHV-4),and equine influenza virus(EIV).The findings indicated that the method was specifically capable of detecting EAV,while the other pathogens tested yielded negative results.The method demonstrated a high degree of specificity.It was employed to detect the standard plasmid cRNA synthesized through in vitro transcription following a 10-fold dilution.The results indicated that the minimum detection limit of the method was 1.6×10^(2) copies/μL,and it exhibited high sensitivity.The coefficient of variation,both within and between groups,was maintained at 1.8%,indicating good reproducibility.In this study,the fluorescence quantitative RT-PCR assay developed was utilized alongside the EAV fluorescence quantitative RT-PCR assay established by previous researchers to analyze a total of 234 clinical samples.Both methods yielded a positive detection rate of 14.1%,and the coincidence rate between the two techniques was found to be 100%.[Conclusion]The fluorescence quantitative RT-PCR assay developed in this study offers a novel approach and concept for the prevention and control of equine viral arteritis(EVA).展开更多
请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同...请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同,相映成趣。它们都可归纳为同一句型结构,即“主语+动词+介词+介词宾语”。在此结构中,主语和介词宾语的位置可以互换,但动词不变,且句意基本相同。Otto展开更多
Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates th...Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates the historical process of discoveringdrugs and gradually accumulating experi-ence by our ancestors in the course of展开更多
My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’...My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’a beautifultree——all kinds of colors. Cars are stopping to, look. You ought tosee it.'展开更多
By the Qing Dynasty^1 present day Xianning Prefecture was well established as a producerof tea. and for much of that period dealt on the international markets. The rise of thePrefecfure’s tea empires was speedy, but ...By the Qing Dynasty^1 present day Xianning Prefecture was well established as a producerof tea. and for much of that period dealt on the international markets. The rise of thePrefecfure’s tea empires was speedy, but histories of the tea towns leave unanswered thequestion where did all the tea come from? This article postulates that the prefecture’s tea originatedand was first cultivated in an area known as Jiu Gong Shan^2 as early as the Jin Dy-展开更多
1.A Visit to Puhading’sTombAlthough you are just a smallhill,you were my entire world as achild.At that time I knew only thatyou were called Muslim Hall,and asI grew older,I began tounderstand your namePerhaps it was...1.A Visit to Puhading’sTombAlthough you are just a smallhill,you were my entire world as achild.At that time I knew only thatyou were called Muslim Hall,and asI grew older,I began tounderstand your namePerhaps it was be-cause my primaryschool was located be-side you or maybe展开更多
GANSU PROVINCE in nor- thwest China retains threegrotto sites considered cul-tural gems of mankind.Mt.MaijiStanding 45 kilometers south-east of Tianshui City,Mt.Maiji iswell known for its ancient grottosdating from 38...GANSU PROVINCE in nor- thwest China retains threegrotto sites considered cul-tural gems of mankind.Mt.MaijiStanding 45 kilometers south-east of Tianshui City,Mt.Maiji iswell known for its ancient grottosdating from 384 to 417.The sitewas expanded in later centuries,making it one of China’s largegroups of grottos and an importantstop for Buddhists on the Silk Road.Cut into Mt.Maiji‘s 100-meter-high cliffs,the 190 grottos,linkedby plank roads,vary in width from31 meters to only 1 meter.展开更多
CHINESE people have accu-mulated a great deal ofexperience in architecture,constantly improving building ma-terials and thus creating uniquebuilding styles.The history of ancient Chinesearchitechtural development can
“LOOMING green moun-tains and runningstreams;grass does notwither and fall,though the autumnhas come to an end in the south.The bright moon arises overTwenty-fourth Bridge.Where doyou teach pure-jade Yangzhouwomen to...“LOOMING green moun-tains and runningstreams;grass does notwither and fall,though the autumnhas come to an end in the south.The bright moon arises overTwenty-fourth Bridge.Where doyou teach pure-jade Yangzhouwomen to play music on bambooflutes?”This poem by Du Mu(803-c.852),a famous poet of thelate Tang Dynasty,is well remem-bered today It made Yangzhou’sTwenty-fourth Bridge Known to la-ter generations.Of many ancientpoems about Twenty-fourth展开更多
Christmas season is the most festive time of the year in the UnitedStates. Students from grammar school through college have one to two weck svacation, beginning shortly before Christmas and ending soon after NewYear ...Christmas season is the most festive time of the year in the UnitedStates. Students from grammar school through college have one to two weck svacation, beginning shortly before Christmas and ending soon after NewYear s. Many families go away for the holidays, perhaps to ski in展开更多
Scientists believe the British custom of throwing tealeaves on the floor before sweeping, to keep downdust, could have bad other health benefits. Tea leaves contain tannic acid and this chemical isknown to reduce the ...Scientists believe the British custom of throwing tealeaves on the floor before sweeping, to keep downdust, could have bad other health benefits. Tea leaves contain tannic acid and this chemical isknown to reduce the effects of allergens--proteinsin the air which give us allergic reactions, such as runnyeyes, wheezing and coughing. Scientists recently discovered that spraying tannic展开更多
In the present English vocabulary there is a group of common wordscoined from the different proper names.The use of a proper names to conveya general idea is termed antonomasia.By the means of antonomasia thenames ori...In the present English vocabulary there is a group of common wordscoined from the different proper names.The use of a proper names to conveya general idea is termed antonomasia.By the means of antonomasia thenames originally used to refer to specific persons.places and somethingelse are now used as common words which carry their own fresh meanings.Inthis short article it is discussed in detail that from what sources thesecommon words are produced.展开更多
基金Sponsored by Program of Housing and Urban-rural Construction Department of Gansu Province(JK2014-22)
文摘Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto Culture Corridor of the Silk Road. Daxiang Mountain Giant Buddha Grottoes is the landmark of Gangu County. The giant Buddha statue stands among cliffs 200 meters high above the ground, and it is the second largest stone-base clay-sculpture Buddha in China. After going through thousands of years, the Giant Buddha Grottoes needs effective and scientific protection. This article was originated from the actual project—The Research on the Construction Technology of Wooden-structure Grotto Eaves in the Daxiang Mountain. This article f inished the rebuilding plan for the Buddha Grotto Eaves on the basis of analyzing historical documents, hole traces, morphological evolution and image demonstration.
基金Supported by Research Project of General Administration of Customs(2022HK126)Youth Science Foundation(2022D01B08).
文摘[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction system was optimized.Standard curves were established,leading to the initial development of the EAV fluorescence quantitative RT-PCR assay.The accuracy,specificity,and sensitivity of this method were subsequently evaluated.[Result]The EAV fluorescence quantitative RT-PCR assay demonstrated optimal performance at an annealing temperature of 61 C,with a final concentration of primer and probe set at 0.6μmol/L.The plasmid standard demonstrated a strong linear correlation with Ct values within the range of 1.6×10^(7)-1.6×10^(2)copies/μL.The equation of the standard curve was determined to be y=-2.68x+32.88,with an R^(2) value of 0.9927.Consequently,the EAV fluorescence quantitative RT-PCR assay was successfully established.The methodology employed was effective in detecting EAV,Theileria equi,equine herpesvirus-1(EHV-1),equine herpesvirus-4(EHV-4),and equine influenza virus(EIV).The findings indicated that the method was specifically capable of detecting EAV,while the other pathogens tested yielded negative results.The method demonstrated a high degree of specificity.It was employed to detect the standard plasmid cRNA synthesized through in vitro transcription following a 10-fold dilution.The results indicated that the minimum detection limit of the method was 1.6×10^(2) copies/μL,and it exhibited high sensitivity.The coefficient of variation,both within and between groups,was maintained at 1.8%,indicating good reproducibility.In this study,the fluorescence quantitative RT-PCR assay developed was utilized alongside the EAV fluorescence quantitative RT-PCR assay established by previous researchers to analyze a total of 234 clinical samples.Both methods yielded a positive detection rate of 14.1%,and the coincidence rate between the two techniques was found to be 100%.[Conclusion]The fluorescence quantitative RT-PCR assay developed in this study offers a novel approach and concept for the prevention and control of equine viral arteritis(EVA).
文摘请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同,相映成趣。它们都可归纳为同一句型结构,即“主语+动词+介词+介词宾语”。在此结构中,主语和介词宾语的位置可以互换,但动词不变,且句意基本相同。Otto
文摘Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates the historical process of discoveringdrugs and gradually accumulating experi-ence by our ancestors in the course of
文摘My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’a beautifultree——all kinds of colors. Cars are stopping to, look. You ought tosee it.'
文摘By the Qing Dynasty^1 present day Xianning Prefecture was well established as a producerof tea. and for much of that period dealt on the international markets. The rise of thePrefecfure’s tea empires was speedy, but histories of the tea towns leave unanswered thequestion where did all the tea come from? This article postulates that the prefecture’s tea originatedand was first cultivated in an area known as Jiu Gong Shan^2 as early as the Jin Dy-
文摘1.A Visit to Puhading’sTombAlthough you are just a smallhill,you were my entire world as achild.At that time I knew only thatyou were called Muslim Hall,and asI grew older,I began tounderstand your namePerhaps it was be-cause my primaryschool was located be-side you or maybe
文摘GANSU PROVINCE in nor- thwest China retains threegrotto sites considered cul-tural gems of mankind.Mt.MaijiStanding 45 kilometers south-east of Tianshui City,Mt.Maiji iswell known for its ancient grottosdating from 384 to 417.The sitewas expanded in later centuries,making it one of China’s largegroups of grottos and an importantstop for Buddhists on the Silk Road.Cut into Mt.Maiji‘s 100-meter-high cliffs,the 190 grottos,linkedby plank roads,vary in width from31 meters to only 1 meter.
文摘CHINESE people have accu-mulated a great deal ofexperience in architecture,constantly improving building ma-terials and thus creating uniquebuilding styles.The history of ancient Chinesearchitechtural development can
文摘“LOOMING green moun-tains and runningstreams;grass does notwither and fall,though the autumnhas come to an end in the south.The bright moon arises overTwenty-fourth Bridge.Where doyou teach pure-jade Yangzhouwomen to play music on bambooflutes?”This poem by Du Mu(803-c.852),a famous poet of thelate Tang Dynasty,is well remem-bered today It made Yangzhou’sTwenty-fourth Bridge Known to la-ter generations.Of many ancientpoems about Twenty-fourth
文摘Christmas season is the most festive time of the year in the UnitedStates. Students from grammar school through college have one to two weck svacation, beginning shortly before Christmas and ending soon after NewYear s. Many families go away for the holidays, perhaps to ski in
文摘Scientists believe the British custom of throwing tealeaves on the floor before sweeping, to keep downdust, could have bad other health benefits. Tea leaves contain tannic acid and this chemical isknown to reduce the effects of allergens--proteinsin the air which give us allergic reactions, such as runnyeyes, wheezing and coughing. Scientists recently discovered that spraying tannic
文摘In the present English vocabulary there is a group of common wordscoined from the different proper names.The use of a proper names to conveya general idea is termed antonomasia.By the means of antonomasia thenames originally used to refer to specific persons.places and somethingelse are now used as common words which carry their own fresh meanings.Inthis short article it is discussed in detail that from what sources thesecommon words are produced.
