Preliminary study on the protective effect of electroacupuncture Neiguan acupoint pretreatment on rats with myocardial ischemia-reperfusion injury:role of the miR-214-3p/NCX1 axis

Background:Ischemia-reperfusion can worsen myocardial damage and increase the risk of death.Studies have revealed that ischemic preconditioning provides the best endogenous protection against myocardial ischemia-reperfusion injury(MIRI),and the principle of electroacupuncture(EA)preconditioning is comparable to that of myocardial ischemic preconditioning adaption.Our earlier research demonstrated that EA pretreatment inhibits the expression of calmodulin-dependent protein kinase IIδ(CaMKIIδ),sodium/calcium exchanger 1(NCX1),and cyclophilin D,hence providing protection against MIRI.However,the exact mechanism is still unknown.The expression of NCX1 mRNA is directly regulated by microRNA-214(miR-214).Moreover,it suppresses the levels of CaMKIIδand cyclophilin D.Whether these variables contribute to EA preconditioning to improve MIRI needs to be investigated,though.This study aimed to preliminarily determine whether EA pretreatment ameliorates MIRI by modulating the miR-214-3p/NCX1 axis.Methods:We used a rat MIRI model to investigate the effect of EA pretreatment on MIRI and the expression of miR-214-3p.In addition,adenovirus injection inhibited miR-214-3p expression in the rat MIRI model,and the influence of EA pretreatment towards MIRI was observed in the context of blocked miR-214-3p expression.Both the myocardial histological abnormalities and the alterations in the ST segment of the rat electrocardiogram were analyzed.NCX1 mRNA,cyclophilin D,and CaMKIIδexpression levels were also analyzed.Results:EA pretreatment improved MIRI.In rats with MIRI,EA administration increased miR-214-3p expression while decreasing NCX1 mRNA,cyclophilin D,and CaMKIIδproteins in cardiac tissues.The beneficial effect of EA pretreatment against MIRI was reversed,coupled with elevated levels of NCX1 mRNA,cyclophilin D,and CaMKIIδprotein expression,when an adenovirus injection disrupted the expression of miR-214-3p.Conclusions:Our findings preliminarily show that EA pretreatment inhibits the expression of NCX1 mRNA,cyclophilin D,and CaMKIIδproteins via miR-214-3p,hence exerting MIRI protection.

Research on Types of Regional Eco-protection Space and Its Protection Countermeasures——A Case Study of Chongzuo City of Guangxi Province in China

Reasonable division of eco-protection space is a vital measure to coordinate the relationship between regional economic development and ecological protection,and to realize regional sustainable development.The paper has concluded the concept of regional eco-protection space which has been divided into ecological space of forbidding development and restricted development.It has discussed methods to divide regional eco-protection space and proposed protection countermeasures for regional eco-protection space.GIS has been adopted to divide eco-protection space of Chongzuo City.The eco-protection space of Chongzuo City covers an overall area of 5,833.94 km2,taking up 34.04% of the total area of Chongzuo City;ecological space of forbidding development covers an area of 1,555.35 km2,taking up 8.97%;the ecological space of restricted development covers an area of 4,344.59 km2,taking up 25.07%.Finally,the protection countermeasures for regional eco-protection space have been proposed.

Study on the Biodiversity Protection System of Eco-garden City

It is a basic requirement of eco-garden city construction to establish a biodiversity protection system,and also a concrete requirement of ecological development for an eco-garden city.Biodiversity protection system of the eco-garden city includes the following contents:clarifying hierarchical requirements of the urban biodiversity protection,that is,classified protection of planning hierarchy and biodiversity hierarchy;achieving biodiversity protection via the planning system;establishing a quantity index system of urban biodiversity protection.Major strategies for establishing the biodiversity protection system were given as below:giving proper ecological planning and design;providing policy and law support;enhancing the construction of humanistic and education environment.Study on biodiversity protection system in eco-garden city may become an important support for the future quality evaluation of urban construction.

Legal System for China's Forest Resource Protection:A Case Study of Poyang Lake Eco-economic Zone

Taking Poyang Lake Eco-economic Zone as an example,we analyzed existing problems in China'forest resource protection from the perspective of legal system.The problems include unclear property right system of forest resource,imperfect compensation mechanism for forest ecology,forest cutting quota scheme not resolutely put into practice,legal supervision of forest protection not implemented,and lack of integration in forest protection laws.Based on these problems,we put forward countermeasures and suggestions:establish clear forest resource property system;improve ecological benefit compensation mechanism in legal form;strictly implement forest cutting quota scheme;strengthen legal supervision of forest protection;and keep legal system integral for forest resource protection.

Research on market-based policy instruments for eco-environmental protection

The selection of policy instruments involves policy efficiency and policy effect.Effective settlement of eco-environment problems in the process of industrialization and implementation of active eco-environment protection depend on the innovation of policy instruments.In this paper,based on a descriptive analysis of the policy instruments for ecoenvironmental protection of our country,combined with the theoretical analysis and the empirical research on current marketization of eco-environmental protection,the authors indicate that eco-environmental problems originate fi'om the damage of eco-environment and the extemalities of eco-service,and that the market-based policy instruments which are helpful to balance the relationship between fight,responsibility and interest in the eco-service are effective measures to eliminate externalities and protect eco-environment.

Preparation of Eco-environmental Protection Bricks from Lake Sludge

Eco-environmental protection hollow bricks manufactured from lake sludge collected from North Lake in Wuhan of China were investigated. At first, the recipes of round sam- pies（diameter in 5 cm and thickness in 1 cm） prepared by pressing molding under a pressure of 8 MPa and firing system were optimized, and properties such as bending strength, compressive strength, total shrinkage rate, water adsorption rate, apparent porosity and bulk density of the fired samples were tested. The experimental results showed that water adsorption rate and apparent porosity decreased, while bulk density increased with firing temperature increasing. Addition of additive can result in the improvement of strength of fired samples. At the same time, fly ash and additive may also broaden the firing temperature range and reduce firing temperature. Toxic characteristic leaching procedure （TLCP） tests of samples also show that the metal leaching level is low. The conditions for manufacturing good quality sample include 80% sludge with 10% additive and 10% flyash fired at 1000 ℃ for 60 min. Bending strength and compressive strength of samples prepared under the conditions are 24.64 MPa, 131.95 MPa, respectively,which are far beyond the requirements of clay solid bricks of CNS. Finally, according to the optimized recipe,the eco-environmental protection hollow bricks with the sizes of 40 mm × 38 mm ×25 mm were made and tested by extrusion molding. Compared with the Chinese National Standards （CNS： GB 13545-2003）, compressive strength, water adsorption and bulk denstiy of the hollow bricks are 11.55 MPa ,14.5% and 986 kg/m^3 when they were fired at 1040 ℃, which are far beyond the require of of CNS. Especially, its water adsorption arrives to standard of high-class products（16.0%） and compressive strength is above the hightest class（MU 10.0）.

Research Review on Construction and Protection of Tibet Eco-environment

Tibet is not only famous ＂ the roof of the world＂ but also is recognized as the ＂ third pole of the earth＂ by the whole world, with very im-portant significance in construction and protection of plateau eco-environment. In Qinghai - Tibet Plateau, there are abundant wild animal and plant resources and a lot of high-mountain mineral resources, and water resources, light resources and characteristic agriculture and animal husbandry resources have broad development and utilization space. When Tibet economy and society improve and develop, eco-environment construction and protection work is also mentioned in the schedule. In this paper, the history, status quo and prospect of Tibet eco-environment protection and development are explored, which could promote the understanding on Tibet eco-environment construction and protection in academic world.

Water resources optimization and eco-environmental protection in Qaidam Basin

In order to realize sustainable development of the arid area of Northwest China, rational water resources exploitation and optimization are primary prerequisites. Based on the essential principle of sustainable development, this paper puts forward a general idea on water resources optimization and eco-environmental protection in Qaidam Basin, and identifies the competitive multiple targets of water resources optimization. By some qualitative methods such as Input-output Model & AHP Model and some quantitative methods such as System Dynamics Model & Produce Function Model, some standard plans of water resources optimization come into being. According to the Multiple Targets Decision by the Closest Value Model, the best plan of water resources optimization, eco-environmental protection and sustainable development in Qaidam Basin is finally decided.

生态保护与乡村振兴双赢生态补偿标准的模拟与分析--以祁连山国家公园为例

生态补偿作为建设生态文明的重要制度保障和推进乡村振兴的重要机制,是实现国家公园生态保护与乡村振兴“双赢”目标的重要途径。其中,补偿标准的制定是实施“双赢”生态补偿机制的关键环节。以祁连山国家公园为例,将生态补偿标准纳入生态保护-乡村振兴复杂系统之中,通过构建系统动力学模型,将不同的发展情景和补偿标准相结合,仿真模拟生态保护-乡村振兴系统未来发展趋势,甄选更适合祁连山国家公园“双赢”目标实现的发展路径和生态补偿标准。研究发现:(1)通过发展路径规划和补偿标准优化,祁连山国家公园能够实现生态保护和乡村振兴“双赢”。在不同的发展情景中,综合协调情景为祁连山国家公园实现生态保护和乡村振兴“双赢”发展的最优方案,该情景能兼顾各子系统之间协调发展的同时,使生态环境和乡村振兴指数达到最大值。(2)综合协调情景下,当补偿标准区间为129.84—149.24元/hm^(2),为“双赢”目标实现下制定生态补偿标准的相对最优方案。(3)经济优先情景下,当补偿标准维持现状(128.55元/hm^(2))不变时,为制定生态补偿标准的相对最优方案。(4)延续现状和生态优先情景下,当补偿标准区间为131.12—173.01元/hm^(2),为制定生态补偿标准的相对最优方案。从系统视角剖析祁连山国家公园“双赢”的演化路径,在一定程度上可以避免做出片面的发展决策,研究结果不仅为祁连山国家公园体制建设和生态补偿政策实施提供行动指南,其研究思路和方案设计对其他国家公园实现“双赢”目标具有一定的借鉴意义。

Comparison of Thermo-Structural Responses for Integrated Thermal Protection Panels with Different Corrugated Core Configurations

To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-layer corrugated core panels were performed. The obtained the temperature,buckling,stress and deflection responses were compared,and the deflection and stress distributions as well as thermal buckling mode at the time were discussed for the considered configurations when the temperature difference between the top and bottom face sheet was maximum. The results demonstrated that the non-orthogonal and hat-stiffened double-layer structures provide superior performance to resist thermal buckling deformation in comparison with other configurations. The useful information is provided for the forthcoming optimization in which thermal buckling is considered as critical design driver.

Effects of natural cerebrolysin on protective proteins and pro-apoptotic molecules in mesenchymal stem cells following beta-amyloid peptide1-40-induced endoplasmic reticulum stress

BACKGROUND： Studies have demonstrated that β-amyloid peptide （Aβ）, a characteristic pathological product of Alzheimer＇s disease （AD）, results in neuronal endoplasmic reticulum stress （ERS）. However, the mechanisms of traditional Chinese medicine against ERS in AD are poorly understood. OBJECTIVE： To measure expression levels of protective proteins （GRP78 and GRP94） of ER molecular partners and pro-apoptotic Caspase-12 ER membrane expression following application of traditional Chinese medicine natural cerebrolysin （NC） to treat Aβ1-40-induced ERS. DESIGN, TIME AND SETTING： A parallel-controlled study was performed at the Institute of Integrated Western and Traditional Chinese Medicine, Shenzhen Hospital of Southern Medical University between September 2006 and November 2008. MATERIALS： Sprague Dawley male rats, 6-8 weeks old, were used to harvest tibial and femoral bone marrow. Isolation and purification of mesenchymal stem cells （MSCs） were established from the whole bone marrow by removing non-adherent cells in primary and passage cultures. Aβ1-40 was provided by Sigma, USA. NC was provided by Shenzhen Institute of Integrated Chinese and Western Medicine, China. NC was predominantly composed of Renshen （Radix Ginseng）, Tianma （Rhizoma Gastrodiae）, and Yinxingye （Ginkgo Leaf） in a proportion of 1 ： 2： 2. Following conventional water extraction technology, an extract （1 ： 20） was prepared. Six adult, male, New Zealand rabbits underwent intragastric administration of NC extract （0.976 g/kg per day） for 1 month to prepare NC-positive serum, and the remaining 6 rabbits received intragastric administration of physiological saline to prepare normal blank serum. METHODS： A total of 500 nmol/L Aβ1-40 was used to establish ERS models of primary cultured MSCs. AD cell models were incubated with different doses of NC-positive serum （2.5%, 5%, and 10%）. MSCs treated with normal blank serum served as normal blank controls. MAIN OUTCOME MEASURES： Reverse transcription-polymerase chain reaction and fluorescent immunocytochemistry were respectively used to measure mRNA and protein expression levels of GRP78, GRP94, and Caspase-12 in MSCs. RESULTS： Following Aβ1-40 exposure, mRNA and protein expression levels of GRP78 and GRP94, as well as Caspase-12, significantly increased （P 〈 0.05）, suggesting successful establishment of ERS models. Following NC-positive serum application, mRNA and protein expression levels of GRP78 and GRP94 in MSCs significantly increased （P 〈 0.05 or P 〈 0.01）. However, mRNA and protein expression levels of Caspase-12 significantly decreased （P 〈 0.05, or P 〈 0.01） compared with the ERS model group. These effects were dose-dependent. CONCLUSION： NC downregulated Caspase-12 expression and upregulated GRP78 and GRP94 expression in MSCs in a dose-dependent manner under the state of Aβ1-40-induced ERS.

A NEW POLYMER-BOUND 1,2-DIOL AS A PROTECTING AGENT FOR SYMMETRICAL DIALDEHYDE

A novel polymer-bound 1,2-diol, 3-polystyrylsulfonyl-1,2-propanediol (6) had been prepared by the reaction of sodium polystyrylsulfinate with allyl bromide, followed by oxidation and. hydrolysis or directly with 3-chloro-1,2-propanediol in the presence of a phase transfer catalyst, n-tetrabutylammonium iodide. The capacity of resin 6 for terephthaidehyde reached 1.43 mmol/g. The aldehydic groups attached to polymer 6 reacted with hydroxylamine hydrochloride or reduced by sodium borohydride giving p-formylbenzaldoxime (yield: 89%)and p-formyl-benzalcohol (yield: 734%), respectively. The high yields of these polymer-supported reactions showed that the polymer 6 possessed the effective isolation of its reactive sites.

Green Design and Planning Resolutions for an Eco-Industrial Town: A Case Study of Polluted Industrial Estate in Rayong Province, Thailand

Map Ta Phut was designated for development in national policy as an Eastern Seaboard Industrial Port. It is located in Rayong Province with growing demand from Eastern Seaboard industries and is heavily affected by serious environmental problems as a'pollution control zone'. While the Joint Standing Committee on Commerce, Industry and Banking, representing the national business sector, claimed that the area generated total revenues of 1.1 trillion baht per year, or 11% of Thailand’s gross domestic product, and employed more than 100,000 workers, the declaration of the area as a pollution control zone severely limited investment and business operations. Thus, controversies arose among the investment business sectors, the residential sector (residents have been affected by a decrease in their quality of life and health) and environmental concern sectors. This paper aims to find an efficient and practical mitigation practice to balance the purposes of the industrial port with protection of surrounding communities and natural resources. The author will apply physical design and planning such as the application of “buffer zones”, “greenbelts”, “set-back”, “green corridor”, “green wall” and “protection strips” along with environmental measurements such as the Air Pollution Tolerance Index (APTI), which can be adapted for pollution protection as best practices of landscape architecture.

基于“山水林田湖草一体化保护”的生态保护修复研究--以广州北部生态涵养区为例

山水林田湖草系统治理是推进生态文明建设的重要方法。文章以广州北部生态涵养区为研究对象,针对该区域的森林资源质量需提升、资源保护力度应加强、森林健康管理待提高3种主要生态问题,提出以综合治理生态环境本底为基础、以增强森林生态系统质量和稳定性为导向的生态保护修复思路,注重生态环境综合治理、森林质量精准提升、生物多样性保护与修复、自然资源科学管理4类13项生态保护修复内容,并通过生态保护修复区划,分析诊断5个功能分区现存问题,提出生态保护修复策略,开展相应的实施内容。

The effect of protective genital care protocol on preventing diaper dermatit development in 0 - 18 month old children using antibiotics

Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of the disease. The aim of this study is find out the effect of protective genital care on reducing the diaper dermatit development in 0 - 18 months old inpatient 0 - 18 month old children who are using antibiotic. The study was conducted as prospective half-experimetal using control-experimental groups. Our samples comprise of 0 - 18 month old children admitted at Dokuz Eylul University Practical and Research Hospital, pediatric clinic between January and May 2009, 41 0 - 18 month old children as a control group were not under protective genital care, and 42 0 - 18 month old children as an experimental group were under the protecion and using vaseline. The mothers of the experimental group 0 - 18 month old children were given education. All 0 - 18 month old children were followed-up 3 - 7 days and the collected data were evaluated in computer using Pearson’s chisquare, Fisher’s exact chi-square and Yatest-corrected chi-square tests. The diaper dermatit develop- ment ratio was 34.1% in the control group and 7.1% in the experimental group