Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

Living environment shaped residents’willingness to pay for ecosystem services in Yangtze River Middle Reaches Megalopolis,China

Profound understanding on the diversity of local residents’willingness to pay(WTP)for ecosystem services(ESs)may aid evaluation of policy impacts by uncovering trade-offs and synergies for achieving sustainable environmental resource management.However,the reasons for the spatial preference heterogeneity of WTP are still unclear.In this study,an extensive survey with 4,580 residents in the Yangtze River Middle Reaches Megalopolis(YRMRM),central China,was carried out to investigate the public’s WTP to maintain and improve ESs.We identified key influencing factors and explored how they shaped the preference heterogeneity in WTP by random forest model and surface fitting method.Our results showed that 81.4% of residents reported WTP for ESs greater than zero.Water supply,waste treatment,food supply and raw material supply were the preferred ESs for residents.Living environment factors(i.e.,extent of farmland,water and forest near the residence)were found as the key drivers of WTP.Proximity to farmland,water and forests resulted in high WTP values for a series of specific ESs.The reasons for the heterogeneity of WTP are(1)the difference in proximity to farmland,water and forest(i.e.,the contributor to ESs),(2)the divergence of local residents’preferences for ESs.This study disclosed the heterogeneity and causes of public preferences in YRMRM,which could help policy and decision makers to develop eco-compensation initiatives towards conservative and sustainable use of the environmental resources.

GPSA:A Greedy Pull-based Scheduling Approach for P2P Live Streaming under Heterogeneous Environments

Pull-based P2P live streaming is a promising solution for the large scale streaming systems, like PPStream, PPlive, due to its high scalability, low cost and high resilience. However, they usually suffer from bad delay performance. In this paper, we seek to improve the delay performance under ensuring video display quality stemming from chunk scheduling. And so we model Pull-based chunk scheduling problem as a multi-objective optimization problem to minimize the video delay and maximize video display quality in the environment of heterogeneous upload bandwidths, heterogeneous and dynamic propagation delays. Finally we put up with a greedy Pull-based scheduling approach(GPSA) to solve the optimization problem. The evaluation shows GPSA can outperform two classical chunk scheduling approaches and adapt to dynamic variance of propagation delays.

Individual alcohol consumption by the population of Russia versus regional living conditions

Background:There are substantial regional differences in drinking alcohol in Russia,both at the population and individual levels.However,the causes of these differences have not been studied yet.Objective:The goal of our study was to examine the effect of regional living conditions on individual alcohol consumption by the population of Russia.Methods:For the analysis,we used data from a cross-sectional epidemiological study conducted in 2013-2014.The final analytical sample included 18,130 people aged 25-64 years.We conducted the interviews face to face,based on which any drinking in the last year,as well as alcohol abuse,were considered as a response.Alcohol abuse was recorded when the respondent consumed 5.75 or more grams of pure ethanol per day(75th percentile of average daily alcohol consumption among alcohol drinkers).The assessment of the regional living conditions was accomplished via integral indexing,which was previously performed based on publicly available data for 2010-2014.Associations were assessed using generalized scoring equations with unchanging standard errors.The associations were expressed by odds ratios(OR)and 95%confidence intervals(C).Results:Deterioration of social conditions and increase in demographic depression in the region of residence increased the odds of any drinking(OR 1.51,95%CI:1.33 to 1.72,P<0.001 and OR 1.22,95%CI:1.05 to 1.41,P=0.oo9,respectively).The odds of alcohol abuse increased with the deterioration of social living conditions and the growth of the industrial development in the region:OR 1.35,95%CI:1.14 to 1.59,P<0.001 and OR 1.16,95%CI:1.05 to 1.28,P=0.002,respectively.Conclusion:Our analysis allowed assessing the impact of the regional living conditions on individual drinking alcohol in the population of Russia.

The Evaluation and Optimization of Satisfaction with the Rural Living Environment in the Southern Terraced Area:A Case Study of the Ziquejie Terrace in Hunan Province

Rural living environment is an important part of the habitat environment science, but there is the serious phenomenon of "favoring city" in current researches on the habitat environment. There is not enough attention paid to the deteriorating rural environment. Taking the case of Ziquejie, coupled with the heritage-abased community characteristics of the rural living environment, we establish the evaluation indicator system of satisfaction with the quality of rural living environment, and use fuzzy analysis method to analyze and process the data obtained from the questionnaire survey, to initially derive the development status and general law of evolution of the rural living environment in Ziquejie. Based on these, we put forth the relevant recommendations for promoting the sustainable development of the rural living environment in Ziquejie.

The Optimization and Thinking about Traditional Living Environment of the Tibetan Gongbu Culture——In the Case of Juemu Village on the Side of the Niyang River

Based on the problems of traditional living environment of the Tibetan Gongbu culture and the concept of inheriting the Tibetan Gongbu culture and the harmonious coexistence of environment and human,living environment design of Juemu Village on the side of the Niyang River is optimized. It adheres to the importance of traditional culture,and especially emphasizes the layout of buildings with Tibetan style and their surrounding,function optimization,and the plant design,thereby exploring and attempting planning method of Tibetan living environment under the background of new rural construction.

Living Environment and Hypercholesterolemia: Case of Mono and Couffo Departments, Benin, in 2015

Introduction: Studies conducted in Benin have often not emphasized the living environment underlying hypercholesterolemia. The objective was to study the prevalence and factors associated with hypercholesterolemia in the Mono and Couffo departments in 2015 with consideration of the living environment. Methods: Descriptive cross-sectional study with an analytical aim that involved 2490 subjects aged 18 - 69 years, selected using a three-stage randomized sampling technique. Data were collected using the Personal Digital Assistant (WHO STEPS instrument) and analyzed according to STEPS recommendations using Epi-Info7.1.5.0 and SPSS20 software. The Chi-square test was used to compare proportions and the difference was considered significant for p < 0.05. Results: Out of 2490 respondents, 60.20% were women. The mean age was 36.14 ± 12.82 years. The prevalence of hypercholesterolemia was 4.7% (95% CI: [3.88 - 5.54]) and lower in the Couffo. Factors associated with hypercholesterolemia after multivariate analysis were harmful alcohol consumption, high blood pressure, type 2 diabetes, obesity and overweight. Conclusions: Living environment was found to be one of the important factors to consider in strategies to control hypercholesterolemia in the Mono and Couffo departments.

Research on Landscape System of Traditional Living Environment in Jingzhou

With the aid of the research method of land landscape,on the basis of briefly analyzing the construction process of regional traditional living environment in Jingzhou,a hierarchical analysis of the regional landscape system was carried out from the aspects of natural landscape,water conservancy construction,agricultural production and urban construction,and the overall pattern of traditional living environment landscape system in Jingzhou was summarized to provide new ideas for the exploration of landscape system.In view of the many problems in the current urban development of Jingzhou,the enlightenment of traditional human settlement experience and wisdom in Jingzhou to the current urban construction of Jingzhou was summarized to provide an example reference for building a living environment that integrates mountains,water and a city.

Study on the Morphological Characteristics of Skin and Hair Coat of Yak and the Adaptability to the Cold Living Environment

Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing.

Green-Color World Creates for You a New Living Environment

Shenzhen Green-Color World LandscapeHorticultural Research and DevelopmentCo., Ltd., is a high and new technologyenterprise. Its operations include cross-breeding grouped flowers, the design and imple-mentation of horticultural projects,

Postischemic Housing Environment on Cerebral Metabolism and Neuron Apoptosis after Focal Cerebral Ischemia in Rats

The purpose of this study was to evaluate the roles of different housing environments in neurological function, cerebral metabolism, cerebral infarction and neuron apoptosis after focal cerebral ischemia. Twenty-eight Sprague-Dawley rats were divided into control group （CG） and cerebral ischemia group, and the latter was further divided into subgroups of different housing conditions： standard environment （SE） subgroup, individual living environment （IE） subgroup, and enriched environment （EE） subgroup. Focal cerebral ischemia was induced by the middle cerebral artery occlusion （MCAO）. Beam walking test was used to quantify the changes of overall motor function. Cerebral infarction and cerebral metabolism were studied by in vivo magnetic resonance imaging and 1H-magnetic resonance spectra, respectively. Neuron necrosis and apoptosis were detected by hematoxylin-eosin and TUNEL staining methods, respectively. The results showed that performance on the beam-walk test was improved in EE subgroup when compared to SE subgroup and IE subgroup. Cerebral infarct volume in IE subgroup was significantly larger than that in SE subgroup （P〈0.05） and EE subgroup （P〈0.05） on day 14 after MCAO. NAA/Cr and Cho/Cr ratios were lower in MCAO groups under different housing conditions as compared to those in CG （P〈0.05）. NAA/Cr ratio was lower in IE subgroup （P〈0.05） and higher in EE subgroup （P〈0.05） than that in SE subgroup. NAA/ Cr ratio in EE was significantly higher than that in IE subgroup （P〈0.05）. Cho/Cr ratio was decreased in MCAO groups as compared to that in CG （P〈0.05）. A significant decrease in normal neurons in cerebral cortex was observed in MCAO groups as compared to CG （P〈0.05）. The amount of normal neurons was less in IE subgroup （P〈0.05）, and more in EE subgroup （P〈0.05） than that in SE subgroup after MCAO. The amotmt of normal neurons in EE subgroup was significantly more than that in IE subgroup after MCAO （P〈0.05）. The ratio of TUNEL-positive neurons in EE was significantly lower than that in SE subgroup （P〈0.05） and IE subgroup （P〈0.05）. Correlation analysis showed that the beam walking test was negatively correlated with NAA/Cr ratio （P〈0.05）. Cerebral infarct volume was negatively correlated with both NAA/Cr ratio （P〈0.01） and Cho/Cr ratio （P〈0.01）. The amount of normal cortical neurons was positively correlated with both NAA/Cr ratio （P〈0.0I） and Cho/Cr ratio （P〈0.05）. The TUNEL-positive neurons showed a negative correlation with both NAA/Cr ratio （P〈0.01） and Cho/Cr ratio （P〈0.01）. This study goes further to show that EE may improve neurological functional deficit and cerebral metabolism, decrease cerebral infarct volume, neuron necrosis and apoptosis, while IE may aggravate brain damage after MCAO.

Distribution of Avian Influenza A Viruses in Poultry-Related Environment and Its Association with Human Infection in Henan, 2016 to 2017

Objective To survey avian influenza A viruses(AIVs) in the environment and explore the reasons for the surge in human H7 N9 cases.Methods A total of 1,045 samples were collected from routine surveillance on poultry-related environments and 307 samples from human H7 N9 cases-exposed environments in Henan from 2016 to2017. The nucleic acids of influenza A(Flu A), H5, H7, and H9 subtypes were detected by real-time polymerase chain reaction.Results A total of 27 H7 N9 cases were confirmed in Henan from 2016 to 2017, 24 had a history of live poultry exposure, and 15 had H7 N9 virus detected in the related live poultry markets(LPMs). About 96%(264/275) Flu A positive-environmental samples were from LPMs. H9 was the main AIV subtype(10.05%) from routine surveillance sites with only 1 H7-positive sample, whereas 21.17% samples were H7-positive in H7 N9 cases-exposed environments. Samples from H7 N9 cases-exposed LPMs(47.56%)had much higher AIVs positive rates than those from routine surveillance sites(12.34%). The H7+H9 combination of mixed infection was 78.18%(43/55) of H7-positive samples and 41.34%(43/104) of H9-positive samples.Conclusion The contamination status of AIVs in poultry-related environments is closely associated with the incidence of human infection caused by AIVs. Therefore, systematic surveillance of AIVs in LPMs in China is essential for the detection of novel reassortant viruses and their potential for interspecies transmission.

Further Morphological Evidence of the Affinity Between Paomu Tree and Fossil Metasequoia and Its Environmental Implication

Leaves of a Metasequoia glyptostroboides tree in Paomu Village,Longshan County,Hunan Province (Paomu Tree) possess"Even Type"cuticles and frill-margined stomata similar to those of fossil Metasequoia by far known but different from all other living Metasequoia trees(common trees).Paomu Tree is thus hypothesized as the real"living fossil",which has been supported by preliminary DNA analyses. Techniques of clearing leaf,epidermis stripping,

Environmental Renewal Strategy of Old Residential Areas:A Case Study of Luochengtou Spinning Machine Yard No.6 of Handan City

Nowadays,China’s urbanization has entered a new stage,paying more attention to the human settlement environment.The urban environment has been greatly improved,with a new look.However,the contradiction between the old city and the new city has gradually become prominent.The old residential area is the basic composition of the old city.Some old residential areas are in disrepair for a long time,and the supporting facilities are imperfect.There are problems such as single style,chaotic layout,hidden quality hazards,etc.,which are difficult to meet the living standards of modern residents.To solve this problem,from the aspects of the levels involved in the renewal and reconstruction of old residential areas,it should repair the building quality,optimize the housing function and increase the residents’happiness.Based on reconstruction of old residential areas in Congtai District of Handan City,Luochengtou Spinning Machine Yard No.6 of Handan City is taken as the research object.Common strategies in the reconstruction of old residential areas are explored,and the existing problems are updated and transformed.The research could provide reference for renewal design of old residential areas.

Designing for Independent Living:The Case Study of an Inclusive Apartment for Persons with Disabilities

Independent living and freedom of choice are closely linked to the social inclusion of PwD(Persons with Disabilities)in society.The paper reports on a recent study focused on investigating and understanding the needs and desires of persons with intellectual disabilities,with reduced mobility and with visual impairments while performing daily activities in order to design user-friendly and inclusive home environments for their life project.The case study concerns an apartment on the ground floor of a building well embedded in a neighborhood urban and social context.It was selected by Anffas Trentino Onlus to train young adults in independent living.Anffas is the National Association of Families of People Intellectual and/or Relational Disabilities,present and operating throughout Italy.Since 2008,important projects have been promoted by Anffas Trentino Onlus aimed at stimulating a personal and housing autonomy in PwD for“during and after us”,or rather when there are no more family members to take care of them.The design process involved a representative focus group of end users.With them and some Anffas staff components,it was possible to implement a participatory design process by sharing and verifying needs,desires,expectations.This approach was adopted in order to jointly develop a design proposal for a new inclusive apartment changing the paradigm from design for to design with,and building awareness both in the end users and designers.

乡村振兴背景下建立农村人居环境长效管护机制的挑战与智慧化进路

全面推进乡村振兴,加快建设宜业宜居乡村更具现实紧迫性,对未来我国农村人居环境的整治成效、治理能力以及持续性管护提出了新要求。数字赋能农村人居环境长效管护治理体系、推动治理能力现代化,使长效管护工作执行便捷化,利于多方管护主体协同共治,创新了长效管护监督新格局。农村人居环境长效管护机制初步探索之期,面临农村地区人居环境基础条件不健全、管护资源匮乏、农民群众参与度较低、管护主体联动困难、人居环境监督不到位等障碍与挑战。针对此种状况,应将数字治理方式引入农村人居环境整治行动中,推进农村人居环境长效管护机制的智慧化设计,充分发挥数字技术对人居环境长效管护工作的推动作用,实现数字赋能农村人居环境生态宜居和美持续化、长效化,进而有效助力乡村振兴。

乡村振兴背景下北部湾地区新农村独栋住宅优化设计研究

改善农村人居环境,建设美丽宜居乡村,是实施乡村振兴战略的一项重要任务。为助力乡村振兴,打造乡村生态宜居住宅,本文以北海市铁山港区扫管龙村的农村住宅为例,立足农村住宅优化设计的影响要素、设计原则、设计意义,结合扫管龙村的基本概况,对该村落的住宅现状进行分析。从该村落所面临的问题点出发,通过理论与实践调研相结合的方式对其新农村独栋住宅的优化设计展开研究,总结出适宜北部湾地区的住宅环境优化策略,为新农村住宅环境的设计理论提供参考。

铸牢中华民族共同体意识下的中华民族生境研究

人类依靠文化在其历史进程中应对所处的自然环境与社会环境,建构起了各民族的生境。我国各民族生境多样性成为中华民族多元一体格局的生态基础,其以文化为纽带在我国各民族的生境中开展其交往交流交融事项,由此使中华各民族的生境形成一个整体,成为中华民族的共有生境。在中华民族生境中达成中华各民族的生态、生计与生命耦合体,这便是铸牢中华民族共同体意识的生境基础,此为构建人类命运共同体的前提。