The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V ...The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.展开更多
Multi-user collaborative editors are useful computer-aided tools to support human-to-human collaboration.For multi-user collaborative editors,selective undo is an essential utility enabling users to undo any editing o...Multi-user collaborative editors are useful computer-aided tools to support human-to-human collaboration.For multi-user collaborative editors,selective undo is an essential utility enabling users to undo any editing operations at any time.Collaborative editors usually adopt operational transformation(OT)to address concurrency and consistency issues.However,it is still a great challenge to design an efficient and correct OT algorithm capable of handling both normal do operations and user-initiated undo operations because these two kinds of operations can interfere with each other in various forms.In this paper,we propose a semi-transparent selective undo algorithm that handles both do and undo in a unified framework,which separates the processing part of do operations from the processing part of undo operations.Formal proofs are provided to prove the proposed algorithm under the well-established criteria.Theoretical analysis and experimental evaluation are conducted to show that the proposed algorithm outperforms the prior OT-based selective undo algorithms.展开更多
文摘The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.
基金National Key R&D Program of China(2017YFB0503004)the National Natural Science Foundation of China(Grant No.62072348)+1 种基金China Postdoctoral Science Foundation(2019M662709)Natural Science Foundation of Hubei Province(2016FC0106305 and 2019CFB627).
文摘Multi-user collaborative editors are useful computer-aided tools to support human-to-human collaboration.For multi-user collaborative editors,selective undo is an essential utility enabling users to undo any editing operations at any time.Collaborative editors usually adopt operational transformation(OT)to address concurrency and consistency issues.However,it is still a great challenge to design an efficient and correct OT algorithm capable of handling both normal do operations and user-initiated undo operations because these two kinds of operations can interfere with each other in various forms.In this paper,we propose a semi-transparent selective undo algorithm that handles both do and undo in a unified framework,which separates the processing part of do operations from the processing part of undo operations.Formal proofs are provided to prove the proposed algorithm under the well-established criteria.Theoretical analysis and experimental evaluation are conducted to show that the proposed algorithm outperforms the prior OT-based selective undo algorithms.
