为快速进行山羊无创伤产前的性别诊断,同时对家畜的遗传缺陷检测和胚胎移植等研究提供科学依据,采用改良酚/氯仿法提取妊娠母羊血浆DNA,通过双重PCR技术扩增雄性性别决定(sex deter-mining region Y,SRY)基因,并以真核生物延伸生长因-子...为快速进行山羊无创伤产前的性别诊断,同时对家畜的遗传缺陷检测和胚胎移植等研究提供科学依据,采用改良酚/氯仿法提取妊娠母羊血浆DNA,通过双重PCR技术扩增雄性性别决定(sex deter-mining region Y,SRY)基因,并以真核生物延伸生长因-子1(elongation factor 1 alpha,EF-1α)基因为内参照进行试验。结果显示,15例孕有雄性胎儿的孕母羊血浆样品中,13例扩增出SRY基因片段,检出率为86.67%(13/15);孕有雌性胎儿的孕母羊血浆中均未检出SRY基因片段,雌性和雄性检测的正确率为92.86%(26/28)。展开更多
Post-transcriptional modifications,including histone modifications and DNA methylation,alter the chromatin landscape to regulate gene expression,thus control various cellular processes in plants.EARLY FLOWERING IN SHO...Post-transcriptional modifications,including histone modifications and DNA methylation,alter the chromatin landscape to regulate gene expression,thus control various cellular processes in plants.EARLY FLOWERING IN SHORT DAYS(EFS)is the major contributor for H3K36 methylation in Arabidopsis and is important for plant development.Here,we find that EFS is expressed in different stages of embryo morphogenesis,and the efs mutant produces larger embryo that results in enlarged seeds.Further analysis reveals that an imprinted gene MOP9.5 is hypomethylated at the promoter region and its expression is derepressed in efs mutant.MOP9.5 promoter is marked by various epigenetic modifications,and we find that following the increase of H3K36me3,H3K27me3 and H3K9me2 levels are reduced in efs mutant.This data indicates an antagonistic regulation between H3K36me3 and DNA methylation,and/or H3K27me3 at MOP9.5.Our results further show that both maternal and paternal EFS alleles are responsible for the seed size regulation,which unraveled a novel function of EFS in plant development.展开更多
基金supported by National Key R&D Program (2016YFA0500800)the National Natural Science Foundation of China (31571322)+2 种基金Tsinghua-Peking Joint Center for Life Sciences1000 Young Talent Program of ChinaS.Shafiq and Wei Xu are supported by the postdoctoral fellowships from Tsinghua-Peking Joint Center for Life Sciences
文摘Post-transcriptional modifications,including histone modifications and DNA methylation,alter the chromatin landscape to regulate gene expression,thus control various cellular processes in plants.EARLY FLOWERING IN SHORT DAYS(EFS)is the major contributor for H3K36 methylation in Arabidopsis and is important for plant development.Here,we find that EFS is expressed in different stages of embryo morphogenesis,and the efs mutant produces larger embryo that results in enlarged seeds.Further analysis reveals that an imprinted gene MOP9.5 is hypomethylated at the promoter region and its expression is derepressed in efs mutant.MOP9.5 promoter is marked by various epigenetic modifications,and we find that following the increase of H3K36me3,H3K27me3 and H3K9me2 levels are reduced in efs mutant.This data indicates an antagonistic regulation between H3K36me3 and DNA methylation,and/or H3K27me3 at MOP9.5.Our results further show that both maternal and paternal EFS alleles are responsible for the seed size regulation,which unraveled a novel function of EFS in plant development.
