Steroid elimination within 24 hours after orthotopic liver transplantation:effectiveness and tolerability

BACKGROUND:Steroids have been the mainstay of immunosuppressive regimen in liver transplantation.However,the use of steroids is associated with various post-transplant complications.This study evaluated the efficacy and safety of reduced immunosuppressive regimen with steroids(steroid elimination within 24 hours post-transplant) in a cohort of Chinese liver transplant recipients.METHODS:Seventy-six patients in line with the selection criteria were enrolled in this prospective study.All patients received anti-IL-2 receptor antibody induction and tacrolimusbased maintenance therapy.The recipients were divided into two groups according to the duration of steroid use:40 transplant in a 3-month withdrawal group and the remaining 36 in a 24-hour elimination group.Recipient survival,postoperative infections,biopsy-proven acute rejection and steroidresistant acute rejection,non-healing wound,recurrence of hepatitis B virus(HBV) and hepatocellular carcinoma(HCC),de novo diabetes,hyperlipidemia and hypertension were assessed in the two groups.RESULTS:There was no significant difference in patient survival,incidence of acute rejection episodes and hyperlipidemia,and recurrence of HBV and HCC between the two groups.However,the incidence rates of post-transplant infection,non-healing wound,de novo diabetes and hypertension were significantly lower in the 24-hour elimination group than in the 3-month withdrawal group(all P values <0.05).CONCLUSION:Under anti-IL-2 receptor antibody induction and tacrolimus-based maintainance,steroid elimination within 24 hours post-transplant is associated with reduced steroidrelated complications without increasing the risk of rejection.

Hematopoiesis reconstitution and anti-tumor effectiveness of Pai-Neng-Da capsule in acute leukemia patients with haploidentical hematopoietic stem cell transplantation

BACKGROUND With the rapid development of haploidentical hematopoietic stem cell transplantation(haplo-HSCT),primary poor graft function(PGF)has become a lifethreatening complication.Effective therapies for PGF are inconclusive.New Chinese patent medicine Pai-Neng-Da(PND)Capsule exerts dual effect in promoting hematopoiesis recovery and regulating immunity.Still,the application of PND capsule in hematopoietic stem cell transplantation,especially in the haplo-HSCT setting,has not yet been reported.AIM To evaluate the role of PND capsule in acute leukemia patients with haplo-HSCT.METHODS We retrospectively collected data of acute leukemia patients who underwent haplo-HSCT at the Affiliated People’s Hospital of Ningbo University between April 1,2015 and June 30,2020.Twenty-nine consecutive patients received oral PND capsule from the sixth day to the first month after haplo-HSCT were included in the PND group.In addition,31 patients who did not receive PND capsule during haplo-HSCT were included in the non-PND group.Subsequently,we compared the therapeutic efficacy according to the western medical evaluation indexes and Chinese medical symptom scores,and the survival between the PND group and the non-PND group,using the chi-square test,Fisher’s exact test,and the Kaplan-Meier method.RESULTS The duration of platelet engraftment was shorter in the PND group than in the non-PND group(P=0.039).The PND group received a lower frequency of red blood cells and platelet transfusions than the non-PND group(P=0.033 and P=0.035,respectively).In addition,PND capsule marginally reduced the rate of PGF(P=0.027)and relapse(P=0.043).After 33(range,4-106)months of follow-up,the 3-year relapse-free survival(P=0.046)and progression-free survival(P=0.049)were improved in the PND group than in the non-PND group.Also,the therapeutic efficacy of the PND group according to Chinese medical symptom scores was significantly better than that of the non-PND group(P=0.022).Moreover,the adverse events caused by PND capsule were mild.Nevertheless,there were no significant differences in the duration of neutrophil engraftment,the risk of infection within 100 days after haplo-HSCT,the acute graft-versus-host disease,or the 3-year overall survival between the two groups.CONCLUSION PND capsule could promote hematopoiesis reconstitution,improve the therapeutic efficacy of Chinese medical symptom scores,present anti-tumor effectiveness,and prolong the survival of acute leukemia patients with haplo-HSCT.

Preclinical safety,effectiveness evaluation,and screening of functional bacteria for fecal microbiota transplantation based on germ-free animals

Fecal microbiota transplantation(FMT),also known as fecal bacterial therapy,is a treatment option that can quickly reconstruct the normal composition of intestinal microbes,and it has a good therapeutic effect on Clostridium difficile infection,as well as on other microecological disorders.However,the causal mechanism of FMT efficacy remains to be clarified,its safety is a major problem,and the standardization and acceptability of FMT need to be improved.This review summarizes its current research status and potential research areas that need to be strengthened,and proposes to clarify the safety of FMT and the causal relationship between FMT and therapeutic effectiveness based on germ-free animals.Meanwhile,the research system is combined with multiomics technology to screen the effective bacteria in FMT,and develop standard,safe,effective and controllable flora of FMT.

Effects of rapamycin in liver transplantation

BACKGROUND: Rapamycin is a potent new immunosuppressant with a mechanism of action that is distinct from that of calcineurin inhibitors, but few clinical data on rapamycin in liver transplantation are available. Hence it is necessary to evaluate the efficacy and side-effects of rapamycin-based immunosuppression in liver transplant patients. METHODS: We retrospectively analysed 39 liver transplantation patients who took rapamycin as an immunosuppressant. This series consisted of 28 patients with hepatocellular carcinoma, 9 patients with chronic fulminant hepatitis, and 2 patients with end-stage liver cirrhosis. Eight patients used rapamycin for monotherapy, and 31 used rapamycin-based immunosuppression. In the 31 patients, 7 patients used rapamycin instead of mycophenolate mofetil to treat acute rejection. RESULTS: In the 28 patients with hepatocellular carcinoma, the one-year survival rate was 67% without any tumor recurrence. The acute rejection in 7 patients was relieved in 1-2 weeks after the administration of rapamycin. All the 8 patients who received rapamycin monotherapy survived for at least 6 months and liver function tests and biopsy showed nothing abnormal. jaundice in 8 patients with chronic rejection was reduced sharply after use of rapamycin. CONCLUSIONS: Rapamycin given alone or in conjunction with calcineurin inhibitors appears to be an effective primary immunosuppressant regimen for orthotopic liver transplantation patients. Further studies are warranted to evaluate the efficacy and side-effect profile of rapamycin in liver transplant patients.

Inhibitory effect of cyclosporin A on the immunological rejection of rats with cerebral hemorrhage following transplantation of nerve growth factors transfected glial cells

BACKGROUND： At present, it has been confirmed that immunological rejection exists in the cell transplantation in brain tissue, the effects of immunosuppressant on the immunological rejection and the survival of grafts in brain cell transplantation are worthy being investigated further. OBJECTIVE： To observe the immunological rejection after transgeneic cell transplantation in treating cerebra hemorrhage in rats, and investigate the interventional effect of cyclosprin. DESIGN ： A randomized controlled study SETTINGS： Second Affiliated Hospital of Xuzhou Medical College; First Affiliated Hospital of Nanjing Medica University. MATERIALS： Thirty-five healthy clean-degree SD rats of 6-8 weeks old were used, weighing 200-250 g, either male or female; The FACSort flow cytometer （American BD Company） and NYD-1000 image analytical system were used, The rat-anti-rat CD4 monoclonal antibody, rat-anti-rat CD8 monoclonal antibody, and rat-anti-rat MHC Ⅱ antigen monoclonal antibody were purchased from Santa Cruz Company; SP and DAB kits were purchased from Beijing Zhongshan Bio-engineering Company. XSP-8C2 light microscope was the product of Shanghai Zousun Optical Instrument, Co.,Ltd, and KYKY-3800B electron microscope was the product of China KYKY Technology Development Co.,Ltd. METHODS ： The experiments were carried out in the animal experimental center of Nanjing Medical University from April to July in 2003. ① Model establishment： The rats were anesthetized, and then the coordinates of left internal capsule were identified, and the needle was withdrawn after 120 μL blood was injected into the internal capsule. Adenoviruses were taken as the carriers, after the astrocytes were successfully transfected by nerve growth factor（NGF） gene, 0.2 mL cell suspension was injected into the sites of cerebral hemorrhage. Thirty successfully established rat models were randomly divided into cyclosporin A group （n=18） and control group, the rats were treated with intraperitoneal injection of cyclosporin A （10 mg/kg per day） intraperitoneal injection of saline of the same dosage from the 1^st day after transplantation, once a day for 7 days continuously.② CD4^＋ and CD4^＋ detection： The CD4^＋ and CD4^＋ T lymphocytes in caudal vein were counted with flow cytometer at 15 days after treatment. ③ Morphological observation in the transplanted sites： The rats were killed and then brain tissues were taken out, the transplanted sites and the structure of the normal brain tissue around the transplanted sites were observed with light and electron microscopes. ④Detections of the infiltration of T lymphocyte subsets and expression of major histocompatibility complex （MHC） Ⅱ antigen in the transplanted sites： The image analysis of immunohistochemical sections was performed with the image analytical system, and the integral optical density （IOD） was taken as the statistical value to observe the infiltration of T lymphocyte subsets and expression of MHC Ⅱ antigen in the transplanted sites, and the normal brain tissue around the transplanted sites were taken as controls. MATN OUTCOME MEASURES： ① Countings of CD4^＋ and CD4^＋ in peripheral blood; ②Results of the morphological observation in the transplanted sites; ③ Infiltration of T lymphocyte subsets and expression of MHC Ⅱ antigen in the transplanted sites RESULTS ： Totally 35 rats were used, and 30 were successfully made into models, 5 died during the treatment, the other 25 were involved in the analysis of results. ① Results of CD4^＋ and CD4^＋ T lymphocytes in pedpherel blood： The percentages of CD4^＋ and CD4^＋ T lymphocytes in the cyclosporin A group were （29.20±3.97）% and （20.65±2,02）%, respectively, which were obviously lower than those in the control group [（47,39±3,01）%, （28.30±2.36）%, t=-4.983, 4.012, P 〈 0.05], and the CDC/CD4^＋ ratio was obviously lower than that in the control group （1,41±0.86, 1,64^＋0.69, t=-3. 871, P〈 0.05）.② Morphological results in the transplanted sites： Under optical and electron microscopes, the survival region of the transplant was round, and it had an unobvious migration region with the normal brain tissues, the grafts had normal cellular form. Infiltrations of lymphocytes and monocytes were observed in both groups, and mainly located in the transplanted sites, and the expression of lymphocytes in the cyclosporin A group was markedly lower than that in the control group, and no above-mentioned changes were observed in the normal brain tissue around the transplanted sites. ③ Results of CD. and CD4^＋ T lymphocytes and expression of MHC Ⅱ antigen in the transplanted sites： The CD4^＋ and CD4^＋ T lymphocytes and expression of MHC Ⅱ antigen in the transplanted sites were observed in both groups. The IOD of CD4^＋ and CD4^＋ antigen positive cells in the cyclosporin A group were obviously lower than those in the control group （1.85±0.38, 1.44^＋0.33; 3.33±0.37, 2.648±0.56, /=-4.122, 4.434, P〈 0.05）, and the IOD of MHC Ⅱantigen positive cells was markedly lower than that in the control group （0.76±0.22, 0.94±0.24, t=3.885, P 〈 0.05）. CONCLUSION： There is immunological rejection in brain tissue after the transplantation of NSC transgeneic glial cells. ② The immunosuppressant of cyclosporin A can reduce the immunological rejection after the cell transplantation.

EFFECTS OF PSEUDOFYPE RETROVIRUS CONTAINING HUMAN N-RAS ANTISENSE GENE ON THE GROWTH OF HUMAN LIVER CANCER LTNM4 TRANSPLANTED IN NUDE MICE

An amphotropic pseudotype retrovirus containing human N-ras antisense gene was constructed and packaged with helper cells. It has been previously demonstrated that the virus did inhibit the growth of human hepatocarcinoma cell line PLC PRF/5 in vitro accompanied with the blockage of p21 expression. Based on these results, further study was carried on to examine the effect of these viruses on the growth of human hepatoma transplanted LTNM4 in nude mice. It has been shown that the retrovirus containing human antisense N-ras gene could inhibit the hepatoma in nude mice at a rate of 78% (P<0.05) as compared with saline control. No inhibition was observed in group treated with retrovirus which contained no N-ras sequence. These results in vivo lend further support that human N-ras antisense gene mediated by retrovirus could block the expression of the relevant oncogene and lead to the inhibition of cancer growth. It also provided the basis for further approaches of gene therapy for human cancer.

Effect of desensitization treatment for highly sensitized uremic patients before kidney transplantation

Objective To explore the feasibility and efficacy of desensitization protocol for highly sensitized renal transplant patients ( HSP ) . Methods Thirty - five HSPs ( HLA class - I panel reactive antibody 2〉50 % ) , including 27 patients with a positive T and/or B cell cy-

Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation

Objective To investigate the effect of adipose stromal vascular fraction cells(SVFs)on the survival rate of fat ransplantation.Methods 0.5mL autologous fat tissue was mixed with: ① DiI-labeled autologous SVFs (Group A);②

富血小板血浆联合自体脂肪颗粒移植修复颞部凹陷

目的:探究富血小板血浆联合脂肪颗粒移植对颞部凹陷患者的填充效果。方法:选择2019年9月-2022年3月笔者医院收治的100例颞部凹陷患者为研究对象,按随机数字表法分为对照组(50例,给予自体脂肪颗粒移植治疗)和观察组(50例,给予富血小板血浆联合自体脂肪颗粒移植治疗),比较两组患者手术效果、手术指标、满意度以及并发症发生情况。结果:治疗后,观察组治疗总优良率为92.00%,高于对照组的76.00%(P<0.05);治疗后,观察组脂肪厚度高于对照组,且观察组平均手术次数及脂肪移植总量低于对照组(P<0.05);观察组并发症总发生率低于对照组(P<0.05);观察组患者评价及医生评价满意率高于对照组(P<0.05)。结论:富血小板血浆联合自体脂肪颗粒移植能提高自体脂肪移植存活率,降低移植次数,填充效果显著。

自体脂肪颗粒移植矫正泪槽畸形的临床效果分析

目的:探讨自体脂肪颗粒移植填充矫正泪槽畸形的临床效果及对其相关并发症进行分析。方法:选取2018年10月-2022年9月在山东省立医院接受诊治的104例泪槽畸形的患者,采用自体脂肪颗粒移植术,术后6个月对患者进行随访,观察和评价手术效果,记录患者出现局部肿胀、瘀斑、术区条索、血肿、感染、脂肪液化坏死、脂肪栓塞等并发症的发生情况。结果:泪沟改善有效率达91.35%,有3例出现肉眼可见的条索,未出现感染、脂肪液化坏死、脂肪栓塞等严重并发症。结论:自体脂肪颗粒移植是纠正泪槽畸形有效和安全的治疗方法。

AIDET沟通模式联合接纳承诺疗法在造血干细胞移植患儿的应用效果评价

目的探讨AIDET沟通模式联合接纳承诺疗法对造血干细胞移植患儿的影响。方法选取2021年1月—2022年12月于北京儿童医院行造血干细胞移植患儿64例作为研究对象,将2021年1月—2021年12月移植患儿32例作为对照组,2022年1月—2022年12月移植的患儿32例作为观察组。对照组给予常规心理护理及宣教,观察组以AIDET沟通模式联合接纳承诺疗法进行干预。比较两组干预前及干预6周后的焦虑情绪水平、治疗依从性及家长满意度水平。结果干预后,两组的焦虑情绪水平均低于干预前,且观察组焦虑情绪水平评分低于对照组(P<0.05);两组的治疗依从性水平均高于干预前,且观察组治疗依从性高于对照组(P<0.05)。结论AIDET沟通模式联合接纳承诺疗法可改善造血干细胞移植患儿的焦虑情绪,降低其焦虑抑郁程度,提高患儿的治疗依从性,值得临床推广和借鉴。

社会支持和心理弹性在等待移植患者疾病家庭负担和生命质量间的中介效应

目的探讨等待移植患者疾病家庭负担和患者生命质量的关系,分析社会支持和心理弹性因素在疾病家庭负担和生命质量之间的中介效应。方法以2019年6月-2021年12月在新疆维吾尔自治区人民医院移植中心住院的190例等待移植的患者为研究对象,采用一般资料调查、心理弹性量表(CD-RISC)、MOS社会支持量表(MOS-SSS)、疾病家庭负担量表(FBS)、生命质量量表(GQOLI-74)对患者进行问卷调查,而后采用结构方程模型分析患者的心理弹性和社会支持在疾病家庭负担和生命质量间的中介效应。结果FBS评分方面,生命质量好组经济负担、家庭日常活动、娱乐活动、家庭成员躯体健康、家庭关系及心理健康6个维度得分及总得分均低于生命质量差组(均P<0.05);生命质量好组心理弹性量表各维度及总得分、社会支持问卷中讯息与情绪性支持、情感性支持及总得分高于生命质量差组(均P<0.05);中介效应模型的总效应为0.91,直接效应为0.22,中介效应为-0.69(均P<0.05),直接效应占24.18%,中介效应占总效应的75.82%,其中,心理弹性占50.55%(0.46),社会支持占25.27%(0.23)。结论等待移植患者疾病家庭负担和患者生命质量之间存在一定关系,患者的心理弹性和社会支持在等待移植患者疾病家庭负担与生命质量间的中介效应明显。

9.5%丙嗪嘧磺隆悬浮剂对移栽水稻田稗草和鸭舌草的防效及安全性研究

2020和2021年在江西省南昌市进行了9.5%丙嗪嘧磺隆SC在15~60 g a.i./hm^(2)处理下及对照药(苄嘧磺隆和五氟磺草胺)的大田杂草防效试验,并评估其对水稻安全性和产量的影响。结果表明,9.5%丙嗪嘧磺隆SC对移栽水稻田稗草和鸭舌草均具有良好的防除效果,60 g a.i./hm^(2)剂量处理后,对稗草的株防效高达92.40%~98.91%,对鸭舌草的株防效为82.62%~89.15%。所有处理对水稻均无药害产生,并实现增产。因此,新型除草剂丙嗪嘧磺隆可有效防除移栽水稻田稗草和鸭舌草,有较好的应用价值。

数字化导板在即刻自体牙移植术中的初步应用研究

目的评价数字化导板在即刻自体牙移植术中的初步应用效果。方法收集2022年10月—2023年10月于新疆医科大学第一附属医院口腔外科门诊就诊,且行自体牙移植术的患者54例,随机分为试验组和对照组,每组27例。对照组按照常规自体牙移植手术操作,试验组在对照组基础上应用数字化导板技术,分别记录两组手术时间(包括拔除患牙和供体牙时间、牙槽窝预备时间以及供体牙离体时间)、移植牙牙根愈合情况和临床疗效。结果试验组平均总手术时间、牙槽窝预备时间和供体牙离体时间分别为(23.77±4.32)、(6.54±1.77)和(2.80±0.57)min,均明显少于对照组(P<0.05);试验组颈部距离偏差、根尖部距离偏差、深度偏差和角度偏差小于对照组,差异具有统计学意义(P<0.05);两组牙根愈合情况和临床疗效比较无统计学差异(P>0.05);试验组3D模型牙试植次数明显少于对照组(P<0.05)。结论数字化导板能够有效减少即刻自体牙移植手术时间,精准预备受区牙槽窝并一次成功植入供牙,减少实际位置与术前设计的偏差距离。

局部带蒂黏膜附着龈重建术和游离龈移植术在口腔种植修复中的疗效

目的探讨局部带蒂黏膜附着龈重建术和游离龈移植术在口腔种植修复中的临床疗效。方法回顾性分析2018年10月至2020年10月收治的92例口腔种植修复患者的病案资料,根据手术治疗方式选择情况的不同,分为重建组(n=46,采取局部带蒂黏膜附着龈重建术)和游离组(n=46,采取游离龈移植术)。评价2种治疗方式的临床效果,比较2组手术前后的菌斑指数、视觉模拟评分(VAS)、出血指数、角化龈宽度。结果2组口腔种植修复患者的种植成功率比较,差异无统计学意义(P>0.05)。重建组术后3、6个月菌斑指数,术后1周VAS评分,术后3个月出血指数与游离组,差异无统计学意义(P>0.05);2组术后菌斑指数、VAS评分、出血指数较术前均显著下降(P<0.05)。重建组术后3、6个月的角化龈宽度低于游离组(P<0.05)。结论局部带蒂黏膜附着龈重建术和游离龈移植术在口腔移植修复中均能达到显著效果,利于种植牙移植固定,且术后菌斑指数、VAS评分、出血指数均显著下降,但后者角化龈宽度移植效果更显著,两种术式各有优劣势,应结合患者具体病情合理选择适宜术式。

巴马香猪原位肾脏移植模型的建立及手术方式效果对比

目的构建猪猪原位肾脏移植模型并探讨不同血管吻合方式的效果以及并发症发生率。方法采用整块供肾切取术获取肾脏,移植肾修整后,行移植肾动静脉与受体猪动静脉端端吻合的原位肾移植。根据血管吻合方式不同,分为传统吻合组(n=12)和显微吻合组(n=12),比较两组动脉吻合时间、静脉吻合时间、总体手术时间、肾脏冷缺血时间、肾脏热缺血时间以及血管相关并发症发生率。结果传统吻合组动脉吻合时间、静脉吻合时间、总体手术时间、肾脏冷缺血时间均低于显微吻合组,传统吻合组血管并发症发生率高于显微吻合组。结论猪猪原位肾脏移植模型具有较强的可操作性且供受者间肾动静脉显微吻合有助于减少移植肾血管并发症。

芒柄花黄素对裸鼠人胃癌细胞SGC7901移植瘤生长的影响及其机制

目的 :探究芒柄花黄素(Form onone tin,Form)对注射人胃癌细胞系SGC7901细胞悬液裸鼠的保护作用和具体机制。方法:建模成功后将裸鼠分为对照组,低、中、高浓度(10、20、40 mg/kg)芒柄花黄素处理组。观察皮下肿瘤,称量湿重,计算出肿瘤湿重抑制率及体积抑制率。Western blot法检测β-catenin、p-β-catenin、CyclinD1、CDK4以及p-Akt、Akt、Bcl-2、Bax、Caspase3蛋白的相对表达量;免疫组织化学方法测定各组裸鼠肿瘤组织中β-catenin、p-β-catenin、CyclinD1、CDK4、Caspase 3、Caspase 9蛋白表达情况。结果 :免疫组织化学方法结果显示β-cate nin、p-β-cate nin、CyclinD1、CDK4表达情况随Form干预浓度增加而减弱;Caspase3、Caspase 9表达随Form干预浓度增加而增强。Western blot结果显示β-Catenin、p-β-Catenin、CyclinD1、CDK4以及p-Akt、Akt、Bcl-2等蛋白的相对表达量随Form干预浓度增加而降低;Caspase3、Bax等蛋白的相对表达量随Form干预浓度增加而上升。结论:Form可以通过抑制Wnt/β-catenin信号通路而抑制胃癌细胞的增殖,同时还可以上调Caspase3来促进胃癌细胞的凋亡。

尿毒症治疗中肾移植与血液透析的长期效果比较

探讨尿毒症患者治疗中采用肾移植与血液透析的长期效果,为尿毒症患者提供更多安全有效的治疗方法。选取在某医院接受治疗的150例尿毒症患者样本,采用随机选样方式将其分为实验组和对照组,每组各75例患者。对照组进行血液透析治疗,实验组进行肾移植治疗,比较两组患者的营养状况、生活质量、睡眠质量及不良情绪情况。结果表明,实验组的营养水平得分显著优于对照组(P<0.05);生活质量得分极显著地高于对照组(P<0.01);实验组的不良情绪得分显著低于对照组(P<0.05),差异具有统计学意义。对尿毒症患者采用肾移植治疗能有效重建患者的肾脏机能并显著提升其生活质量。但受肾源紧张等因素的限制,血液透析仍然是治疗尿毒症最常用的替代疗法。实际应用中,应根据患者的具体情况选择最适宜的治疗方案,以达到最佳的治疗效果。

角膜缘干细胞移植术联合翼状胬肉切除术对翼状胬肉患者的临床效果

目的探讨临床治疗翼状胬肉疾病采用角膜缘干细胞移植术、翼状胬肉切除术联合方式的效果。方法选择2016年3月1日至2019年9月30日常州市第七人民医院眼科收诊治疗的翼状胬肉患者98例进行研究,按照随机数表法分为两组,对照组(49例)单纯采用翼状胬肉切除术治疗;观察组(49例)在对照组基础上联合角膜缘干细胞移植术治疗。比较两组治疗效果,分别记录各组患者治疗所用时间,比较组间差异性。观察两组患者术后产生的并发症表现,统计比较总发生率。结果观察组翼状胬肉患者经联合术式治疗后,总有效率明显高于对照组,疾病复发率低于对照组,差异有统计学意义(P<0.05)。观察组创口愈合及住院治疗总时间均低于对照组,差异有统计学意义(P<0.05)。观察组术后产生的创面水肿等并发症总发生率低于对照组,差异有统计学意义(P<0.05)。结论临床治疗翼状胬肉可在翼状胬肉切除术基础上联用角膜缘干细胞移植术式,相比单纯切除治疗效果更佳,术后患者复发率更低且并发症少,值得运用推广。