Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We rece...Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.展开更多
香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细...香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细胞排入到腹腔中，经历96h后仍能受精孵化，其受精率与孵化率分别还有10．83％和0．53％。展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000)。
文摘Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.
文摘香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细胞排入到腹腔中，经历96h后仍能受精孵化，其受精率与孵化率分别还有10．83％和0．53％。
