Electro-bioremediation is an innovative method to remedy organic-polluted soil. However, the principle of electrokinetic technology enhancing the function of microbes, especially the relationship of electric intensity...Electro-bioremediation is an innovative method to remedy organic-polluted soil. However, the principle of electrokinetic technology enhancing the function of microbes, especially the relationship of electric intensity and biodegradation efficiency, is poorly investigated. Petroleum was employed as a target organic pollutant at a level of 50 g/kg （mass of petroleum/mass of dry soil）. A direct current power supply was used for tests with a constant direct current electric voltage （1.0 V/cm）. The petroleum concentrations Were measured at 3275-3285 nm after extraction using hexane, the group composition of crude oil was analyzed by column chromatography. The water content of soil was kept 25% （m/m）. The results indicated the degradation process was divided into two periods： from day 1 to day 40, from day 41 to day 100. The treatment of soil with an appropriate electric field led the bacteria to have a persistent effect in the whole period of 100 days. The highest biodegradation efficiency of 45.5% was obtained after treatment with electric current and bacteria. The electric-bioremediation had a positive effect on alkane degradation. The degradation rate of alkane was 1.6 times higher in the soil exposed to electric current than that treated with bacteria for 100 days. A proper direct current could stimulate the microbial activities and accelerate the biodegradation of petroleum. There was a positive correlation between the electric intensities and the petroleum bioremediation efficiencies with a coefficient of 0.9599.展开更多
基金supported by the Water Pollution Control and Management Key Project of Science and Technology of China (No. 2008ZX07208-800)
文摘Electro-bioremediation is an innovative method to remedy organic-polluted soil. However, the principle of electrokinetic technology enhancing the function of microbes, especially the relationship of electric intensity and biodegradation efficiency, is poorly investigated. Petroleum was employed as a target organic pollutant at a level of 50 g/kg （mass of petroleum/mass of dry soil）. A direct current power supply was used for tests with a constant direct current electric voltage （1.0 V/cm）. The petroleum concentrations Were measured at 3275-3285 nm after extraction using hexane, the group composition of crude oil was analyzed by column chromatography. The water content of soil was kept 25% （m/m）. The results indicated the degradation process was divided into two periods： from day 1 to day 40, from day 41 to day 100. The treatment of soil with an appropriate electric field led the bacteria to have a persistent effect in the whole period of 100 days. The highest biodegradation efficiency of 45.5% was obtained after treatment with electric current and bacteria. The electric-bioremediation had a positive effect on alkane degradation. The degradation rate of alkane was 1.6 times higher in the soil exposed to electric current than that treated with bacteria for 100 days. A proper direct current could stimulate the microbial activities and accelerate the biodegradation of petroleum. There was a positive correlation between the electric intensities and the petroleum bioremediation efficiencies with a coefficient of 0.9599.
