Existing strategies for bone defect repair are difficult to monitor.Smart scaffold materials that can quantify the efficiency of new bone formation are important for bone regeneration and monitoring.Carbon nanotubes(C...Existing strategies for bone defect repair are difficult to monitor.Smart scaffold materials that can quantify the efficiency of new bone formation are important for bone regeneration and monitoring.Carbon nanotubes(CNT)have promising bioactivity and electrical conductivity.In this study,a noninvasive and intelligent monitoring scaffold was prepared for bone regeneration and monitoring by integrating carboxylated CNT into chemically cross-linked carboxymethyl chitosan hydrogel.CNT scaffold(0.5%w/v)demonstrated improved mechanical properties with good biocompatibility and electrochemical responsiveness.Cyclic voltammetry and electrochemical impedance spectroscopy of CNT scaffold responded sensitively to seed cell differentiation degree in both cellular and animal levels.Interestingly,the CNT scaffold could make up the easy deactivation shortfall of bone morphogenetic protein 2 by sustainably enhancing stem cell osteogenic differentiation and new bone tissue formation through CNT roles.This research provides new ideas for the development of noninvasive and electrochemically responsive bioactive scaffolds,marking an important step in the development of intelligent tissue engineering.展开更多
基金supported by the National Natural Science Foundation of China(81972530,62171275,82000879)the National Key R&D Project(2018YFC1106100 and 2018YFC1106101)+1 种基金Fund for Excellent Young Scholars of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYYQ001)the Science and Technology Commission of Shanghai(20DZ2270800).
文摘Existing strategies for bone defect repair are difficult to monitor.Smart scaffold materials that can quantify the efficiency of new bone formation are important for bone regeneration and monitoring.Carbon nanotubes(CNT)have promising bioactivity and electrical conductivity.In this study,a noninvasive and intelligent monitoring scaffold was prepared for bone regeneration and monitoring by integrating carboxylated CNT into chemically cross-linked carboxymethyl chitosan hydrogel.CNT scaffold(0.5%w/v)demonstrated improved mechanical properties with good biocompatibility and electrochemical responsiveness.Cyclic voltammetry and electrochemical impedance spectroscopy of CNT scaffold responded sensitively to seed cell differentiation degree in both cellular and animal levels.Interestingly,the CNT scaffold could make up the easy deactivation shortfall of bone morphogenetic protein 2 by sustainably enhancing stem cell osteogenic differentiation and new bone tissue formation through CNT roles.This research provides new ideas for the development of noninvasive and electrochemically responsive bioactive scaffolds,marking an important step in the development of intelligent tissue engineering.