The demand for Electronic Shelf Labels(ESL),according to the Internet of Things(IoT)paradigm,is expected to grow considerably in the immediate future.Various wireless communication standards are currently contending t...The demand for Electronic Shelf Labels(ESL),according to the Internet of Things(IoT)paradigm,is expected to grow considerably in the immediate future.Various wireless communication standards are currently contending to gain an edge over the competition and provide the massive connectivity that will be required by a world in which everyday objects are expected to communicate with each other.Low-Power Wide-Area Networks(LPWANs)are continuously gaining momentum among these standards,mainly thanks to their ability to provide long-range coverage to devices,exploiting license-free frequency bands.The main theme of this work is one of the most prominent LPWAN technologies,LoRa.The purpose of this research is to provide long-range,less intermediate node,less energy dissipation,and a cheaper ESL system.Much research has already been done on designing the LoRaWAN network,not capable to make a reliable network.LoRa is using different gateways to transmit the same data,collision,data jamming,and data repetition are expected.According to the transmission behavior of LoRa,50%of data is lost.In this paper,the Improved Backoff Algorithm with synchronization technique is used to decrease overlapping and data loss.Besides,the improved Adaptive Data Rate algorithm(ADR)avoids the collision in concurrently transmitted data by using different Spreading Factors(SFs).The allocation of SF has the main role in designing LoRa based network to minimize the impact of the intra-interference,cost function,and Euclidean distance.For this purpose,the K-means machine learning algorithm is used for clustering.The data rate model is using an intra-slicing technique based on Maximum Likelihood Estimation(MLE).The data rate model includes three critical communication slices,High Critical Communication(HCC),Medium Critical Communication(MCC),and Low Critical Communication(LCC),having the specified number of End devices(EDs),payload budget delay,and data rate.Finally,different combinations of gateways are used to build ESL for 200 electronic shelf labels.展开更多
Site-directed spin-labeling with continuous wave electron paramagnetic resonance spectroscopy was used to monitor autoproteolysis of HIV-1 protease, an enzyme essential for viral maturation. Two protein constructs wer...Site-directed spin-labeling with continuous wave electron paramagnetic resonance spectroscopy was used to monitor autoproteolysis of HIV-1 protease, an enzyme essential for viral maturation. Two protein constructs were examined, namely subtype F and the circulating recombinant form CRF01_A/E. As the protease undergoes self-cleavage, protein unfolds and small peptide fragments containing the spin label are generated, which collectively give rise to a sharp spectral component that is easily discernable in the high-field resonance line in the EPR spectrum. By monitoring the intensity of this spectral component over time, the autoproteolytic stability of each construct was characterized under various conditions. Data were collected for samples stored at 4 °C, 25 °C, and 37 °C, and on a subtype F HIV-1 protease sample stored at 25 °C and containing the FDA-approved protease inhibitor Tipranavir. As expected, the rate of autoproteolysis decreased as the storage temperature was lowered. Minimal autoproteolysis was seen for the sample that contained Tipranavir, providing direction for future spectroscopic studies of active protease samples. When compared to standard methods of monitoring protein degradation such as gel electrophoresis or chromatographic analyses, spin-labeling with CW EPR offers a facile, real-time, non-consuming way to monitor autoproteolysis or protein degradation. Additionally, mass spectrometry studies revealed that the N-termini of both constructs are sensitive to degradation and that the sites of specific autoproteolysis vary.展开更多
In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 indi...In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24 were injected with 3H-thymidine a DNA precurson, another 10 groups consisting of 3 individuals, total 30, were injected with 3H-uridine a RNA precursor, and another 10 groups of 30 individuals were injected with 3H-leucine a protein precursor, total 90 individuals. Then, all the animals were sacrificed 1 hr after the injections and the liver tissues, the lung tissues, the kidney tissues, the testis and ovary tissues, the adrenal tissues were taken out, fixed and processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, ten photographs in respective groups, numbers of mitochondria per cell profile area, numbers of labeled mitochondria per cell and the mitochondrial labeling index (LI) labeled with 3H-thymidine showing DNA synthesis, LI labeled with 3H-uridine showing RNA synthesis, and LI labeled with 3H-leucine showing protein synthesis, in various organs, were counted and the results in various organs in respective aging groups were compared, respectively. From the results, it was demonstrated that the numbers of mitochondria in hepatocytes as well as in 3 zones of the adrenal cortex, the zona glomerulosa, fasciculata and reticularis of mice at various ages increased from fetal day 19 to postnatal month 1 due to development and aging of animals. On the other hand, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA syntheses incorporating 3H-thymidine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 ones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. Moreover, some other organs such as the lung and the testis were also review and discussed. From the results, it was shown that the activity of intramitochnodrial DNA synthesis, RNA synthesis, and protein synthesis in hepatocytes, adrenal cortical cells, pulmonary cells and testicular cells in developing and aging mice as well as some other organs increased and decreased due to development and aging.展开更多
基金This work is supported by the National Natural Science Foundation of China(61702020)Beijing Natural Science Foundation(4172013)Beijing Natural Science Foundation-Haidian Primitive Innovation Joint Fund(L182007).
文摘The demand for Electronic Shelf Labels(ESL),according to the Internet of Things(IoT)paradigm,is expected to grow considerably in the immediate future.Various wireless communication standards are currently contending to gain an edge over the competition and provide the massive connectivity that will be required by a world in which everyday objects are expected to communicate with each other.Low-Power Wide-Area Networks(LPWANs)are continuously gaining momentum among these standards,mainly thanks to their ability to provide long-range coverage to devices,exploiting license-free frequency bands.The main theme of this work is one of the most prominent LPWAN technologies,LoRa.The purpose of this research is to provide long-range,less intermediate node,less energy dissipation,and a cheaper ESL system.Much research has already been done on designing the LoRaWAN network,not capable to make a reliable network.LoRa is using different gateways to transmit the same data,collision,data jamming,and data repetition are expected.According to the transmission behavior of LoRa,50%of data is lost.In this paper,the Improved Backoff Algorithm with synchronization technique is used to decrease overlapping and data loss.Besides,the improved Adaptive Data Rate algorithm(ADR)avoids the collision in concurrently transmitted data by using different Spreading Factors(SFs).The allocation of SF has the main role in designing LoRa based network to minimize the impact of the intra-interference,cost function,and Euclidean distance.For this purpose,the K-means machine learning algorithm is used for clustering.The data rate model is using an intra-slicing technique based on Maximum Likelihood Estimation(MLE).The data rate model includes three critical communication slices,High Critical Communication(HCC),Medium Critical Communication(MCC),and Low Critical Communication(LCC),having the specified number of End devices(EDs),payload budget delay,and data rate.Finally,different combinations of gateways are used to build ESL for 200 electronic shelf labels.
文摘Site-directed spin-labeling with continuous wave electron paramagnetic resonance spectroscopy was used to monitor autoproteolysis of HIV-1 protease, an enzyme essential for viral maturation. Two protein constructs were examined, namely subtype F and the circulating recombinant form CRF01_A/E. As the protease undergoes self-cleavage, protein unfolds and small peptide fragments containing the spin label are generated, which collectively give rise to a sharp spectral component that is easily discernable in the high-field resonance line in the EPR spectrum. By monitoring the intensity of this spectral component over time, the autoproteolytic stability of each construct was characterized under various conditions. Data were collected for samples stored at 4 °C, 25 °C, and 37 °C, and on a subtype F HIV-1 protease sample stored at 25 °C and containing the FDA-approved protease inhibitor Tipranavir. As expected, the rate of autoproteolysis decreased as the storage temperature was lowered. Minimal autoproteolysis was seen for the sample that contained Tipranavir, providing direction for future spectroscopic studies of active protease samples. When compared to standard methods of monitoring protein degradation such as gel electrophoresis or chromatographic analyses, spin-labeling with CW EPR offers a facile, real-time, non-consuming way to monitor autoproteolysis or protein degradation. Additionally, mass spectrometry studies revealed that the N-termini of both constructs are sensitive to degradation and that the sites of specific autoproteolysis vary.
文摘In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24 were injected with 3H-thymidine a DNA precurson, another 10 groups consisting of 3 individuals, total 30, were injected with 3H-uridine a RNA precursor, and another 10 groups of 30 individuals were injected with 3H-leucine a protein precursor, total 90 individuals. Then, all the animals were sacrificed 1 hr after the injections and the liver tissues, the lung tissues, the kidney tissues, the testis and ovary tissues, the adrenal tissues were taken out, fixed and processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, ten photographs in respective groups, numbers of mitochondria per cell profile area, numbers of labeled mitochondria per cell and the mitochondrial labeling index (LI) labeled with 3H-thymidine showing DNA synthesis, LI labeled with 3H-uridine showing RNA synthesis, and LI labeled with 3H-leucine showing protein synthesis, in various organs, were counted and the results in various organs in respective aging groups were compared, respectively. From the results, it was demonstrated that the numbers of mitochondria in hepatocytes as well as in 3 zones of the adrenal cortex, the zona glomerulosa, fasciculata and reticularis of mice at various ages increased from fetal day 19 to postnatal month 1 due to development and aging of animals. On the other hand, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA syntheses incorporating 3H-thymidine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 ones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. Moreover, some other organs such as the lung and the testis were also review and discussed. From the results, it was shown that the activity of intramitochnodrial DNA synthesis, RNA synthesis, and protein synthesis in hepatocytes, adrenal cortical cells, pulmonary cells and testicular cells in developing and aging mice as well as some other organs increased and decreased due to development and aging.
