Objective： To study the role and effect of Schwann cells （SCs） remyelination in contused spinal cord. Methods： Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal ti...Objective： To study the role and effect of Schwann cells （SCs） remyelination in contused spinal cord. Methods： Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal tis- sues of the injury site at 1 week after the spinal cords were contused. At 6 weeks, the spinal cords were removed for cryosections, semithin sections and ultrathin sections, and then immunocytochemical staining of myelin basic protein （MBP）, P0 protein （P0） and S 100 protein （S 100） was carried out on the cryosections. Qualitative and semiquantitative analyses were performed on the cryosections and semithin sections. Ultrastructure ofmyelinated fibers was observed on the ultrathin sections under electron microscope. Results： Transplanted SCs and myelinated fibers im- munocytochemically labeled by MBP, PO as well as S 100 distributed in whole injured area. The quantity of myeli- nated fibers labeled by the three myelin proteins showed no statistical difference, however, which was significantly larger than that of controls. On the semithin sections, the experi- mental group demonstrated more myelinated fibers in the injured area than the controls, but the fibers had smaller diameter and thinner myelin sheath under electron microscope. Conclusion： SCs can promote regeneration of injured nerve fibers and enhance remyelination, which may be his- tological basis of SCs-mediated functional repair of injured spinal cords.展开更多
文摘Objective： To study the role and effect of Schwann cells （SCs） remyelination in contused spinal cord. Methods： Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal tis- sues of the injury site at 1 week after the spinal cords were contused. At 6 weeks, the spinal cords were removed for cryosections, semithin sections and ultrathin sections, and then immunocytochemical staining of myelin basic protein （MBP）, P0 protein （P0） and S 100 protein （S 100） was carried out on the cryosections. Qualitative and semiquantitative analyses were performed on the cryosections and semithin sections. Ultrastructure ofmyelinated fibers was observed on the ultrathin sections under electron microscope. Results： Transplanted SCs and myelinated fibers im- munocytochemically labeled by MBP, PO as well as S 100 distributed in whole injured area. The quantity of myeli- nated fibers labeled by the three myelin proteins showed no statistical difference, however, which was significantly larger than that of controls. On the semithin sections, the experi- mental group demonstrated more myelinated fibers in the injured area than the controls, but the fibers had smaller diameter and thinner myelin sheath under electron microscope. Conclusion： SCs can promote regeneration of injured nerve fibers and enhance remyelination, which may be his- tological basis of SCs-mediated functional repair of injured spinal cords.
