Gaoyou and Jinding ducks with different growth rates were selected to conduct the test, and a comparative study was carried out on the expression pattern of liver insulin-like growth factor 1 （IGF-1） mRNA and its co...Gaoyou and Jinding ducks with different growth rates were selected to conduct the test, and a comparative study was carried out on the expression pattern of liver insulin-like growth factor 1 （IGF-1） mRNA and its correlation with body weight in the early embryonic stage and hatching stage. The expression pattern of hepatic IGF-lmRNA of ducks at embryonic ages of 13, 17, 21,25, 27 and 0 -7 days after hatching were monitored by real-time fluorescent quantitative PCR. The results showed that expression of duck hepatic 1GF-1 mRNA could be detected at the embryonic age of 13. Body weight and hepatic IGF-1 mRNA expression of ducks both showed an increasing trend during the early development. The expression of hepatic IGF-1 mRNA of Gaoyou ducks at embryonic ages of 13, 21 and on the 7^th day after hatching was extremely significantly higher than that of Jinding ducks （P 〈 0.01 ）, which was consistent with the changes of daily weight gain and absolute growth rate. The changes of daily weight gain and hepatic IGF-I mRNA expression both showed extremely significant specificity of the breeds and time during the entire observation （P 〈 0.01）, and which was extremely significantly affected by the interaction of the breeds and time （P 〈 0.01）. The analysis on breeds and gender manifested that the change of body weight was consistent with that of hepatic IGF-I mRNA expression, which showed an extremely significant positive correlation （P 〈 0.01 ）. The results indicated that hepatic IGF-1 mRNA expression played an important role during the early development of ducks.展开更多
基金Supported by National Natural Science Foundation of China(31172194)Jiangsu Science and Technology Support Program(BE2011329)Yangzhou City Technology Support Program(20122078)
文摘Gaoyou and Jinding ducks with different growth rates were selected to conduct the test, and a comparative study was carried out on the expression pattern of liver insulin-like growth factor 1 （IGF-1） mRNA and its correlation with body weight in the early embryonic stage and hatching stage. The expression pattern of hepatic IGF-lmRNA of ducks at embryonic ages of 13, 17, 21,25, 27 and 0 -7 days after hatching were monitored by real-time fluorescent quantitative PCR. The results showed that expression of duck hepatic 1GF-1 mRNA could be detected at the embryonic age of 13. Body weight and hepatic IGF-1 mRNA expression of ducks both showed an increasing trend during the early development. The expression of hepatic IGF-1 mRNA of Gaoyou ducks at embryonic ages of 13, 21 and on the 7^th day after hatching was extremely significantly higher than that of Jinding ducks （P 〈 0.01 ）, which was consistent with the changes of daily weight gain and absolute growth rate. The changes of daily weight gain and hepatic IGF-I mRNA expression both showed extremely significant specificity of the breeds and time during the entire observation （P 〈 0.01）, and which was extremely significantly affected by the interaction of the breeds and time （P 〈 0.01）. The analysis on breeds and gender manifested that the change of body weight was consistent with that of hepatic IGF-I mRNA expression, which showed an extremely significant positive correlation （P 〈 0.01 ）. The results indicated that hepatic IGF-1 mRNA expression played an important role during the early development of ducks.
