In recent years,with the improvement of the requirements of road performance,modified emulsified asphalts with better performance has gradually replaced the emulsified asphalt and become the primary material for road ...In recent years,with the improvement of the requirements of road performance,modified emulsified asphalts with better performance has gradually replaced the emulsified asphalt and become the primary material for road maintenance.This paper introduces the modified emulsified asphalt materials commonly used in pavement maintenance projects,definitions and modified mechanisms of polymerized styrene butadiene rubber(SBR)modified emulsified asphalt,styrene butadiene styrene block polymer(SBS)modified emulsified asphalt and waterborne epoxy resin(WER)modified emulsified asphalt are summarized.The analysis focused on comparing the effects of modifiers,preparation process,auxiliary additives,and other factors on the performance of modified emulsified asphalt.In this paper,it is considered that the greatest impact on the performance of emulsified asphalt is the modifier,emulsifier mainly affects the speed of breaking the emulsion,stabilizers on the basic performance of emulsified asphalt evaporative residue is small;and when the modifier is distributed in the asphalt in a network,the dosage at this time is the recommended optimum dosage.Finally,this study recommends that in the future,the polymer-asphalt compatibility can be improved through composite modification,chemical grafting and other methods to continue to develop broader applicability and better performance of modified emulsified asphalt.展开更多
The current energy crisis could be alleviated by enhancing energy generation using the abundant biomass waste resources. Agricultural and forest wastes are the leading organic waste streams that can be transformed int...The current energy crisis could be alleviated by enhancing energy generation using the abundant biomass waste resources. Agricultural and forest wastes are the leading organic waste streams that can be transformed into useful alternative energy resources. Pyrolysis is one of the technologies for converting biomass into more valuable products, such as bio-oil, bio-char, and syngas. This work investigated the production of bio-oil through batch pyrolysis technology. A fixed bed pyrolyzer was designed and fabricated for bio-oil production. The major components of the system include a fixed bed reactor, a condenser, and a bio-oil collector. The reactor was heated using a cylindrical biomass external heater. The pyrolysis process was carried out in a reactor at a pressure of 1atm and a varying operating temperature of 150˚C, 250˚C, 350˚C to 450˚C for 120 minutes. The mass of 1kg of coconut fiber was used with particle sizes between 2.36 mm - 4.75 mm. The results show that the higher the temperature, the more volume of bio-oil produced, with the highest yield being 39.2%, at 450˚C with a heating rate of 10˚C/min. The Fourier transformation Infrared (FTIR) Spectroscopy analysis was used to analyze the bio-oil components. The obtained bio-oil has a pH of 2.4, a density of 1019.385 kg/m<sup>3</sup>, and a calorific value of 17.5 MJ/kg. The analysis also showed the presence of high-oxygenated compounds;carboxylic acids, phenols, alcohols, and branched oxygenated hydrocarbons as the main compounds present in the bio-oil. The results inferred that the liquid product could be bestowed as an alternative resource for polycarbonate material production.展开更多
Objective:To explore the effect of emulsified isoflurane(EI)on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein expression.Methods:Cardiac muscle anoxiareoxygenation damage model was ...Objective:To explore the effect of emulsified isoflurane(EI)on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein expression.Methods:Cardiac muscle anoxiareoxygenation damage model was established with culture in vitro neonatal rat cardiomyocytes.The cardiomyocytes were divided into control group,model group,fat emulsion group and EI group.The cardiomyocytes apoptosis rates and lactic dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)index standardization were detected after relevant treatment The expression of apoptosis-related proteins Bel-2,Bax and Caspase-3 were detected with Western blot approach.Results:After hypoxia/reoxygenation(H/R)model was treated by EI,the cells apoptosis rate decreased and was dramatically below the fat emulsion group(P<0.05),Cardiomyocytes biochemical index detection presented that,compared with the control group that the LDH activity and MDA content dramatically increased(P<0.05),while the SOD activity notably decreased(P<0.05);compared with the H/R group,the SOD activity of the fat emulsion group and EI group increased(P<0.05);while the LDH activity and MDA content decreased(P<0.05).And the change of the EI group was more remarkable than the fat emulsion group(P<0.05).The Western blot analysis presented that,compared with the control group,the Bcl-2 protein expression of the other groups significantly decreased(P<0.05),the expressions of Bax protein and Caspase-3protein increased significantly(P<0.05);compared with H/R group,cardiomyocytes Bc1-2protein expression of EI group increased significantly(P<0.05),the expressions of Bax protein and Caspase-3 protein decreased significantly(P<0.05),and the change of EI group was more remarkable than the fat emulsion group(P<0.05).Conclusions:EI can inhabit the apoptosis of anoxia-reoxygenation damage model cardiomyocytes,and may he related to the up-regulation of expression of Bcl-2 and down-regulation of expression of Caspase-3 protein.展开更多
The epoxy resin polymer cement mortars with excellent performances were made up through modifying ordinary Portland cement with emulsified epoxy and micro fine slag.The microstructure of the epoxy resin polymer cemen...The epoxy resin polymer cement mortars with excellent performances were made up through modifying ordinary Portland cement with emulsified epoxy and micro fine slag.The microstructure of the epoxy resin polymer cement materials was studied and their hydration and hardening characteristics were discussed by means of modern analysis measures such as SEM,XRD and Hg intrusion micromeritics.The experimental results indicate that the series effects of water reducing,density,pozzolanicity,filling and solidification crosslinking through the action together with epoxy organism and micro fine slag endowed cement based materials with perfect performances.The main hydration products in the system are C S H gel and hydrated calcium aluminate.At later age,AFt can be in existence,and no Ca(OH) 2 is found.When epoxy resin is solidified,the organism is in a network structure.In the micro pore structure of hydrated cement with modified epoxy and fine slag,big harmful pores were fewer,more harmless abundant micro pores were and the possible pore radius was smaller than that of ordinary Portland cement.展开更多
The polyacrylate latexes were synthesized via pre-emulsified and semi-continuous seeded emulsion polymerization technology when conventional surfactant or polymerizable surfactant was used as emulsifiers. The resultan...The polyacrylate latexes were synthesized via pre-emulsified and semi-continuous seeded emulsion polymerization technology when conventional surfactant or polymerizable surfactant was used as emulsifiers. The resultant latexes and their films were characterized with the contact angle determinator and rheometer. Effect of the polymerizable surfactant on water resistance, stability and rheology of the latex was studied. Results show that the water resistance of film is increased first then decreased with the increase of the amount of the polymerizable surfactant. There exists the optimum value of the amount of the polymerizable surfactant for the water resistance of the film. In comparison with the latex prepared with the conventional surfactant, both the mechanical stability and the freezing-thaw stability of the latex are improved when the polymerizable surfactant is used during the course of the emulsion polymerization. The resultant latex has rheological properties of pseudo-plastic fluid and belongs to non-Newtonian fluid.展开更多
Autophagy plays essential roles in cell survival.However,the functions and regulation of the autophagy-related proteins Atg5,LC3B,and Beclin 1 during anesthetic-induced developmental neurotoxicity remain unclear.This ...Autophagy plays essential roles in cell survival.However,the functions and regulation of the autophagy-related proteins Atg5,LC3B,and Beclin 1 during anesthetic-induced developmental neurotoxicity remain unclear.This study aimed to understand the autophagy pathways and mechanisms that affect neurotoxicity,induced by the anesthetic emulsified isoflurane,in rat fetal neural stem cells.Fetal neural stem cells were cultured,in vitro,and neurotoxicity was induced by emulsified isoflurane treatment.The effects of pretreatment with the autophagy inhibitors 3-methyladenine and bafilomycin and the effects of transfection with small interfering RNA against ATG5(siRNA-Atg5)were observed.Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,and apoptosis was assessed using flow cytometry.Ultrastructural changes were analyzed through transmission electron microscopy.The levels of the autophagy-related proteins LC3B,Beclin 1,Atg5,and P62 and the pro-apoptosis-related protein caspase-3 were analyzed using western blot assay.The inhibition of cell proliferation and that of apoptosis rate increased after treatment with emulsified isoflurane.Autophagolysosomes,monolayer membrane formation due to lysosomal degradation,were observed.The autophagy-related proteins LC3B,Beclin 1,Atg5,and P62 and caspase-3 were upregulated.These results confirm that emulsified isoflurane can induce toxicity and autophagy in fetal neural stem cells.Pre-treatment with 3-methyladenine and bafilomycin increased the apoptosis rate in emulsified isoflurane-treated fetal neural stem cells,which indicated that the complete inhibition of autophagy does not alleviate emulsified isoflurane-induced fetal neural stem cell toxicity.Atg5 expression was decreased significantly by siRNA-Atg5 transfection,and cell proliferation was inhibited.These results verify that the Atg5 autophagy pathway can be regulated to maintain appropriate levels of autophagy,which can inhibit the neurotoxicity induced by emulsified isoflurane anesthetic in fetal neural stem cells.展开更多
Magnetic reduced graphene oxide(MRGO) nanocomposite was prepared by the chemical coprecipitation method and applied as adsorbent for removing emulsified oil from oily wastewater. SEM, TEM, XRD, FT-IR, VSM and other an...Magnetic reduced graphene oxide(MRGO) nanocomposite was prepared by the chemical coprecipitation method and applied as adsorbent for removing emulsified oil from oily wastewater. SEM, TEM, XRD, FT-IR, VSM and other analytical methods were utilized to characterize the prepared MRGO. The adsorption performance of MRGO was evaluated under different initial adsorbate concentration, MRGO dosage, temperature, and pH value of the solution. The adsorption kinetics and isotherms were investigated. In addition, the MRGO repeatability was also tested. It was found that almost 65%of emulsified oil were removed by MRGO in the first 15 min. The MRGO adsorption capacity and efficiency for removal of adsorbate reached 335.85 mg/g and 92.52% within 60 min, respectively. The adsorption capacity reduced with an increasing MRGO dosage, while increased with the increase of emulsified oil concentration. The adsorption performance of MRGO in the alkaline environment was lower than that in the acidic environment. The adsorption data could well fit to the pseudosecondorder model. The Langmuir model could well describe the isotherm data. The MRGO adsorption capacity was still more than 236.1 mg/g at the sixth regeneration cycle.展开更多
Diesel fuel has been known as the most effective fuel but it is known as a fuel which produces harmful emissions. Later, emulsified diesel fuel was introduced as a better solution but there is no sufficient research d...Diesel fuel has been known as the most effective fuel but it is known as a fuel which produces harmful emissions. Later, emulsified diesel fuel was introduced as a better solution but there is no sufficient research data concerning combustion of emulsified fuel. The present work carried out a simulation of non-surfactant emulsified diesel fuel where composition of water in emulsion varied from 0% to 20% to determine the ratio of water to diesel which is more effective in reducing the exhaust emissions especially NOx. For this simulation,5% of water in diesel without surfactant was able to reduce NOx?up to 35%. It was shown that as the percentage of water increases, the power from that fuel combustion reduces.展开更多
Corn cob is a naturally renewable material with developed micropore and hydrophobic characteristics, which enables it to show good oil adsorption capacity. In order to improve oil adsorption capacity, corn cob was mod...Corn cob is a naturally renewable material with developed micropore and hydrophobic characteristics, which enables it to show good oil adsorption capacity. In order to improve oil adsorption capacity, corn cob was modified with lauric acid and ethanediol. The structure of raw and modified corn cob was investigated using Fourier transform infrared(FTIR) spectroscopy, scanning electron microscopy(SEM), Brunauer-Emmett-Teller(BET) method, thermogravimetric analysis(TGA) and Ze Ta potential analyzer. The effects of p H level, adsorption time, adsorbent dosage, and initial oil concentration on oil absorbency of corn cob were studied. The results indicate that the modification significantly improved the lipophilicity of corn cob, making the modified corn cob with much better adsorption capacity on oil absorbency. Compared with raw corn cob, the maximum saturated adsorption capacity of modified corn cob is 16.52 mg/g at p H 5, and the increasing percentage is found to be 141%, which indicates that the modification causes a better adsorption capacity for oil removal. In addition, due to high oil adsorption capacity, affordable price and low secondary pollution, the modified corn cob could be considered promising alternative for the traditional oil adsorbent to clean up the emulsified oily water.展开更多
Design of a robust catalyst with high activity but the low cost for the hydrodeoxygenation(HDO) of biooils is of great importance to bring the biorefinery concept into reality.In this study,density functional theory(D...Design of a robust catalyst with high activity but the low cost for the hydrodeoxygenation(HDO) of biooils is of great importance to bring the biorefinery concept into reality.In this study,density functional theory(DFT) calculation was adopted to analyze the optimal location of Ni on MoO_(3-x) containing oxygen vacancy,and the corresponding result demonstrated that metallic Ni cluster located at the neighborhood of oxygen vacancies would significantly evoke HDO activity.Enlightened by DFT results,NiMoO_(4) was first hydrothermally synthesized and then employed to fabricate Ni-MoO_(3-x) catalyst via a low-temperature reduction,where Ni escaped from NiMoO_(4) and was reduced to its metallic state.Such an evolution of Ni species also induced the formation of oxygen vacancies around metallic Ni cluster.In the HDO of p-cresol,Ni-MoO_(3-x) exhibited high activity with a complete conversion and a methylcyclohexane selectivity of 99.4% at 150℃.Moreover,the catalyst showed good versatility in catalyzing HDO of diverse lignin-derived oxygenates and lignin oil.2D HSQC NMR,gas chromatograph and elemental analysis of the lignin oil demonstrated the high deoxygenation efficiency and saturation of the benzene ring over Ni-MoO_(3-x).In the upgrading of crude lignin oil,the deoxygenation degree was up to 99%,and the overall carbon yield of the naphthenes was as high as 69.4%.Importantly,the structures and carbon numbers of the naphthene products are similar to jet fuel-range cycloalka nes,which are expected to have a high density that can be blended into jet fuel to raise the range(or payload) of airplanes.This work demonstrates the feasibility for improving the targeted catalytic reactivity by rational tailoring the catalyst structure under the guidance of theoretical analysis,and provides an energy-efficient route for the upgrading of lignin crude oil into valuable naphthenes.展开更多
Emulsifier is an important component of emulsified skin care BASE(matrix),and the common influencing factors affecting the skin feel of BASE are:emollient,thickeners,and emulsifier.The choice of emulsifier is crucial ...Emulsifier is an important component of emulsified skin care BASE(matrix),and the common influencing factors affecting the skin feel of BASE are:emollient,thickeners,and emulsifier.The choice of emulsifier is crucial to the skin feel of cosmetics.In this paper,12 classical emulsifiers were selected and 13 combinations of them were made.Thirteen creams were designed using the same formulation structure,their physicochemical indexes were determined,and their skin feel was tested and evaluated using five expert panel scores,with the test site being the hand.Physicochemical parameters included pH and viscosity.Skin feel indicators include:stickiness,silkiness,whiteheads,greasiness at the end of application,absorption,softness after use,etc.The BASE formulations of the creams were tested and evaluated by five experts.Recommendations were also made for the selection of emulsifier combinations in the design of cream BASE formulations.展开更多
目的:开发基于大豆分离蛋白(soybean protein isolate,SPI)的新型乳化剂。方法:采用限制性酶解结合糖基化处理对SPI进行结构修饰,研究协同改性对SPI乳化特性的影响。结果:SPI水解物(soybean protein isolate hydrolysate,SPIH)中的相对...目的:开发基于大豆分离蛋白(soybean protein isolate,SPI)的新型乳化剂。方法:采用限制性酶解结合糖基化处理对SPI进行结构修饰,研究协同改性对SPI乳化特性的影响。结果:SPI水解物(soybean protein isolate hydrolysate,SPIH)中的相对分子质量较大组分(F30)的乳化性最佳,且糖基化反应4 h的F30-葡聚糖轭合物乳化稳定性相对最好。相较于SPI,SPIH与F30,F30-葡聚糖轭合物稳定的乳液表现出最低的初始平均粒径,并且具有最佳的贮藏稳定性。当pH接近SPI等电点或体系处于高盐浓度时,所有乳液均出现不稳定聚集现象。与SPI相比,SPIH和F30稳定乳液的聚集程度更高,而F30-葡聚糖轭合物由于共价结合的葡聚糖提供了额外的空间位阻和亲水性,使得轭合物稳定的乳液能够耐受离子强度和温度的变化,在不利环境条件下表现出更高的抵抗力。结论:限制性酶解结合糖基化改性是开发SPI基乳化配料的潜在可靠途径。展开更多
基金The authors acknowledge the financial support from National Natural Science Foundation of China(No.51968006).
文摘In recent years,with the improvement of the requirements of road performance,modified emulsified asphalts with better performance has gradually replaced the emulsified asphalt and become the primary material for road maintenance.This paper introduces the modified emulsified asphalt materials commonly used in pavement maintenance projects,definitions and modified mechanisms of polymerized styrene butadiene rubber(SBR)modified emulsified asphalt,styrene butadiene styrene block polymer(SBS)modified emulsified asphalt and waterborne epoxy resin(WER)modified emulsified asphalt are summarized.The analysis focused on comparing the effects of modifiers,preparation process,auxiliary additives,and other factors on the performance of modified emulsified asphalt.In this paper,it is considered that the greatest impact on the performance of emulsified asphalt is the modifier,emulsifier mainly affects the speed of breaking the emulsion,stabilizers on the basic performance of emulsified asphalt evaporative residue is small;and when the modifier is distributed in the asphalt in a network,the dosage at this time is the recommended optimum dosage.Finally,this study recommends that in the future,the polymer-asphalt compatibility can be improved through composite modification,chemical grafting and other methods to continue to develop broader applicability and better performance of modified emulsified asphalt.
文摘The current energy crisis could be alleviated by enhancing energy generation using the abundant biomass waste resources. Agricultural and forest wastes are the leading organic waste streams that can be transformed into useful alternative energy resources. Pyrolysis is one of the technologies for converting biomass into more valuable products, such as bio-oil, bio-char, and syngas. This work investigated the production of bio-oil through batch pyrolysis technology. A fixed bed pyrolyzer was designed and fabricated for bio-oil production. The major components of the system include a fixed bed reactor, a condenser, and a bio-oil collector. The reactor was heated using a cylindrical biomass external heater. The pyrolysis process was carried out in a reactor at a pressure of 1atm and a varying operating temperature of 150˚C, 250˚C, 350˚C to 450˚C for 120 minutes. The mass of 1kg of coconut fiber was used with particle sizes between 2.36 mm - 4.75 mm. The results show that the higher the temperature, the more volume of bio-oil produced, with the highest yield being 39.2%, at 450˚C with a heating rate of 10˚C/min. The Fourier transformation Infrared (FTIR) Spectroscopy analysis was used to analyze the bio-oil components. The obtained bio-oil has a pH of 2.4, a density of 1019.385 kg/m<sup>3</sup>, and a calorific value of 17.5 MJ/kg. The analysis also showed the presence of high-oxygenated compounds;carboxylic acids, phenols, alcohols, and branched oxygenated hydrocarbons as the main compounds present in the bio-oil. The results inferred that the liquid product could be bestowed as an alternative resource for polycarbonate material production.
基金supported by the Scientific Research Foundation of Health Department of Human Province(B2009-011)
文摘Objective:To explore the effect of emulsified isoflurane(EI)on apoptosis of anoxia-reoxygenation neonatal rat cardiomyocytea and relevant protein expression.Methods:Cardiac muscle anoxiareoxygenation damage model was established with culture in vitro neonatal rat cardiomyocytes.The cardiomyocytes were divided into control group,model group,fat emulsion group and EI group.The cardiomyocytes apoptosis rates and lactic dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)index standardization were detected after relevant treatment The expression of apoptosis-related proteins Bel-2,Bax and Caspase-3 were detected with Western blot approach.Results:After hypoxia/reoxygenation(H/R)model was treated by EI,the cells apoptosis rate decreased and was dramatically below the fat emulsion group(P<0.05),Cardiomyocytes biochemical index detection presented that,compared with the control group that the LDH activity and MDA content dramatically increased(P<0.05),while the SOD activity notably decreased(P<0.05);compared with the H/R group,the SOD activity of the fat emulsion group and EI group increased(P<0.05);while the LDH activity and MDA content decreased(P<0.05).And the change of the EI group was more remarkable than the fat emulsion group(P<0.05).The Western blot analysis presented that,compared with the control group,the Bcl-2 protein expression of the other groups significantly decreased(P<0.05),the expressions of Bax protein and Caspase-3protein increased significantly(P<0.05);compared with H/R group,cardiomyocytes Bc1-2protein expression of EI group increased significantly(P<0.05),the expressions of Bax protein and Caspase-3 protein decreased significantly(P<0.05),and the change of EI group was more remarkable than the fat emulsion group(P<0.05).Conclusions:EI can inhabit the apoptosis of anoxia-reoxygenation damage model cardiomyocytes,and may he related to the up-regulation of expression of Bcl-2 and down-regulation of expression of Caspase-3 protein.
文摘The epoxy resin polymer cement mortars with excellent performances were made up through modifying ordinary Portland cement with emulsified epoxy and micro fine slag.The microstructure of the epoxy resin polymer cement materials was studied and their hydration and hardening characteristics were discussed by means of modern analysis measures such as SEM,XRD and Hg intrusion micromeritics.The experimental results indicate that the series effects of water reducing,density,pozzolanicity,filling and solidification crosslinking through the action together with epoxy organism and micro fine slag endowed cement based materials with perfect performances.The main hydration products in the system are C S H gel and hydrated calcium aluminate.At later age,AFt can be in existence,and no Ca(OH) 2 is found.When epoxy resin is solidified,the organism is in a network structure.In the micro pore structure of hydrated cement with modified epoxy and fine slag,big harmful pores were fewer,more harmless abundant micro pores were and the possible pore radius was smaller than that of ordinary Portland cement.
基金Zhejiang Provincial Natural Science Foundation of China(No.Y4100152)
文摘The polyacrylate latexes were synthesized via pre-emulsified and semi-continuous seeded emulsion polymerization technology when conventional surfactant or polymerizable surfactant was used as emulsifiers. The resultant latexes and their films were characterized with the contact angle determinator and rheometer. Effect of the polymerizable surfactant on water resistance, stability and rheology of the latex was studied. Results show that the water resistance of film is increased first then decreased with the increase of the amount of the polymerizable surfactant. There exists the optimum value of the amount of the polymerizable surfactant for the water resistance of the film. In comparison with the latex prepared with the conventional surfactant, both the mechanical stability and the freezing-thaw stability of the latex are improved when the polymerizable surfactant is used during the course of the emulsion polymerization. The resultant latex has rheological properties of pseudo-plastic fluid and belongs to non-Newtonian fluid.
基金financially supported by the National Natural Science Foundation of China,No.81401279(to ZYY)the Natural Science Foundation of Shanghai,China,No.18ZR1443100(to ZYY)+2 种基金the Innovation Center of Translational Medicine Collaboration,Shanghai Jiao Tong University School of Medicine of China,No.TM201729(to ZYY)the Youth Talent Fund of International Peace Maternity and Child Health Hospital,Shanghai Jiao Tong University School of Medicine of China in 2014(to ZYY)the“WUXIN”Project of International Peace Maternity and Child Health Hospital,Shanghai Jiao Tong University School of Medicine of China in 2019,No.2018-38(to ZYY)。
文摘Autophagy plays essential roles in cell survival.However,the functions and regulation of the autophagy-related proteins Atg5,LC3B,and Beclin 1 during anesthetic-induced developmental neurotoxicity remain unclear.This study aimed to understand the autophagy pathways and mechanisms that affect neurotoxicity,induced by the anesthetic emulsified isoflurane,in rat fetal neural stem cells.Fetal neural stem cells were cultured,in vitro,and neurotoxicity was induced by emulsified isoflurane treatment.The effects of pretreatment with the autophagy inhibitors 3-methyladenine and bafilomycin and the effects of transfection with small interfering RNA against ATG5(siRNA-Atg5)were observed.Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,and apoptosis was assessed using flow cytometry.Ultrastructural changes were analyzed through transmission electron microscopy.The levels of the autophagy-related proteins LC3B,Beclin 1,Atg5,and P62 and the pro-apoptosis-related protein caspase-3 were analyzed using western blot assay.The inhibition of cell proliferation and that of apoptosis rate increased after treatment with emulsified isoflurane.Autophagolysosomes,monolayer membrane formation due to lysosomal degradation,were observed.The autophagy-related proteins LC3B,Beclin 1,Atg5,and P62 and caspase-3 were upregulated.These results confirm that emulsified isoflurane can induce toxicity and autophagy in fetal neural stem cells.Pre-treatment with 3-methyladenine and bafilomycin increased the apoptosis rate in emulsified isoflurane-treated fetal neural stem cells,which indicated that the complete inhibition of autophagy does not alleviate emulsified isoflurane-induced fetal neural stem cell toxicity.Atg5 expression was decreased significantly by siRNA-Atg5 transfection,and cell proliferation was inhibited.These results verify that the Atg5 autophagy pathway can be regulated to maintain appropriate levels of autophagy,which can inhibit the neurotoxicity induced by emulsified isoflurane anesthetic in fetal neural stem cells.
基金funded by the Major National Science and Technology Projects of China(Grant No.2016ZX05040-003)the Top Talents Project of China University of Petroleum(grant No.2015011)
文摘Magnetic reduced graphene oxide(MRGO) nanocomposite was prepared by the chemical coprecipitation method and applied as adsorbent for removing emulsified oil from oily wastewater. SEM, TEM, XRD, FT-IR, VSM and other analytical methods were utilized to characterize the prepared MRGO. The adsorption performance of MRGO was evaluated under different initial adsorbate concentration, MRGO dosage, temperature, and pH value of the solution. The adsorption kinetics and isotherms were investigated. In addition, the MRGO repeatability was also tested. It was found that almost 65%of emulsified oil were removed by MRGO in the first 15 min. The MRGO adsorption capacity and efficiency for removal of adsorbate reached 335.85 mg/g and 92.52% within 60 min, respectively. The adsorption capacity reduced with an increasing MRGO dosage, while increased with the increase of emulsified oil concentration. The adsorption performance of MRGO in the alkaline environment was lower than that in the acidic environment. The adsorption data could well fit to the pseudosecondorder model. The Langmuir model could well describe the isotherm data. The MRGO adsorption capacity was still more than 236.1 mg/g at the sixth regeneration cycle.
文摘Diesel fuel has been known as the most effective fuel but it is known as a fuel which produces harmful emissions. Later, emulsified diesel fuel was introduced as a better solution but there is no sufficient research data concerning combustion of emulsified fuel. The present work carried out a simulation of non-surfactant emulsified diesel fuel where composition of water in emulsion varied from 0% to 20% to determine the ratio of water to diesel which is more effective in reducing the exhaust emissions especially NOx. For this simulation,5% of water in diesel without surfactant was able to reduce NOx?up to 35%. It was shown that as the percentage of water increases, the power from that fuel combustion reduces.
基金Project(51174017)supported by the National Natural Science Foundation of China
文摘Corn cob is a naturally renewable material with developed micropore and hydrophobic characteristics, which enables it to show good oil adsorption capacity. In order to improve oil adsorption capacity, corn cob was modified with lauric acid and ethanediol. The structure of raw and modified corn cob was investigated using Fourier transform infrared(FTIR) spectroscopy, scanning electron microscopy(SEM), Brunauer-Emmett-Teller(BET) method, thermogravimetric analysis(TGA) and Ze Ta potential analyzer. The effects of p H level, adsorption time, adsorbent dosage, and initial oil concentration on oil absorbency of corn cob were studied. The results indicate that the modification significantly improved the lipophilicity of corn cob, making the modified corn cob with much better adsorption capacity on oil absorbency. Compared with raw corn cob, the maximum saturated adsorption capacity of modified corn cob is 16.52 mg/g at p H 5, and the increasing percentage is found to be 141%, which indicates that the modification causes a better adsorption capacity for oil removal. In addition, due to high oil adsorption capacity, affordable price and low secondary pollution, the modified corn cob could be considered promising alternative for the traditional oil adsorbent to clean up the emulsified oily water.
基金supported by the National Key R&D Program of China (2022YFB3805401, 2019YFC1905300)the National Natural Science Foundation of China (22178297)+1 种基金the Hunan Provincial Natural Science Foundation (2022JJ40425, 2022JJ40432)the Process Intensification and Green Chemical Engineering Innovation Team of Hunan Province。
文摘Design of a robust catalyst with high activity but the low cost for the hydrodeoxygenation(HDO) of biooils is of great importance to bring the biorefinery concept into reality.In this study,density functional theory(DFT) calculation was adopted to analyze the optimal location of Ni on MoO_(3-x) containing oxygen vacancy,and the corresponding result demonstrated that metallic Ni cluster located at the neighborhood of oxygen vacancies would significantly evoke HDO activity.Enlightened by DFT results,NiMoO_(4) was first hydrothermally synthesized and then employed to fabricate Ni-MoO_(3-x) catalyst via a low-temperature reduction,where Ni escaped from NiMoO_(4) and was reduced to its metallic state.Such an evolution of Ni species also induced the formation of oxygen vacancies around metallic Ni cluster.In the HDO of p-cresol,Ni-MoO_(3-x) exhibited high activity with a complete conversion and a methylcyclohexane selectivity of 99.4% at 150℃.Moreover,the catalyst showed good versatility in catalyzing HDO of diverse lignin-derived oxygenates and lignin oil.2D HSQC NMR,gas chromatograph and elemental analysis of the lignin oil demonstrated the high deoxygenation efficiency and saturation of the benzene ring over Ni-MoO_(3-x).In the upgrading of crude lignin oil,the deoxygenation degree was up to 99%,and the overall carbon yield of the naphthenes was as high as 69.4%.Importantly,the structures and carbon numbers of the naphthene products are similar to jet fuel-range cycloalka nes,which are expected to have a high density that can be blended into jet fuel to raise the range(or payload) of airplanes.This work demonstrates the feasibility for improving the targeted catalytic reactivity by rational tailoring the catalyst structure under the guidance of theoretical analysis,and provides an energy-efficient route for the upgrading of lignin crude oil into valuable naphthenes.
文摘Emulsifier is an important component of emulsified skin care BASE(matrix),and the common influencing factors affecting the skin feel of BASE are:emollient,thickeners,and emulsifier.The choice of emulsifier is crucial to the skin feel of cosmetics.In this paper,12 classical emulsifiers were selected and 13 combinations of them were made.Thirteen creams were designed using the same formulation structure,their physicochemical indexes were determined,and their skin feel was tested and evaluated using five expert panel scores,with the test site being the hand.Physicochemical parameters included pH and viscosity.Skin feel indicators include:stickiness,silkiness,whiteheads,greasiness at the end of application,absorption,softness after use,etc.The BASE formulations of the creams were tested and evaluated by five experts.Recommendations were also made for the selection of emulsifier combinations in the design of cream BASE formulations.
文摘目的:开发基于大豆分离蛋白(soybean protein isolate,SPI)的新型乳化剂。方法:采用限制性酶解结合糖基化处理对SPI进行结构修饰,研究协同改性对SPI乳化特性的影响。结果:SPI水解物(soybean protein isolate hydrolysate,SPIH)中的相对分子质量较大组分(F30)的乳化性最佳,且糖基化反应4 h的F30-葡聚糖轭合物乳化稳定性相对最好。相较于SPI,SPIH与F30,F30-葡聚糖轭合物稳定的乳液表现出最低的初始平均粒径,并且具有最佳的贮藏稳定性。当pH接近SPI等电点或体系处于高盐浓度时,所有乳液均出现不稳定聚集现象。与SPI相比,SPIH和F30稳定乳液的聚集程度更高,而F30-葡聚糖轭合物由于共价结合的葡聚糖提供了额外的空间位阻和亲水性,使得轭合物稳定的乳液能够耐受离子强度和温度的变化,在不利环境条件下表现出更高的抵抗力。结论:限制性酶解结合糖基化改性是开发SPI基乳化配料的潜在可靠途径。