Changes of the Transcriptional Levels of Molecules Associated with Endogenous Antigen Processing and Presentation in Porcine Skin-derived Dendritic Cells Infected with PCV2 in vivo

[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.

A lunar time scale from the perspective of the Moon’s dynamic evolution

A geologic time scale is a chronological system that separates the geological strata of a planetary body into different units in temporal sequence and shows its progressive evolution.The time scale of the Moon was established a half-century ago during the telescopic-early Apollo exploration era,using data with limited spatial coverage and resolution.The past decades have seen a wide array of studies,which have significantly extended our understanding of global lunar geologic evolution.Based on a comprehensive review of lunar evolution with respect to the dynamical changes,we propose two major updates to the current lunar time scale paradigm to include the evolution of both endogenic and exogenic dynamic forces now known to have influenced early lunar history.Firstly,based on the temporal interplay of exogenic and endogenic processes in altering the Moon,we defined three Eon/Eonothem-level units to represent three dynamical evolutionary phases.Secondly,the pre-Nectarian System is redefined and divided as the magma ocean-era Magma-oceanian System and the following Aitkenian System beginning with the South Pole-Aitken basin.The ejecta of this basin,Das Formation,was deposited on the primordial lunar crust as the oldest stratum produced from exogenic processes.The updated lunar time scale,facilitated by the post-Apollo exploration and research advances,provides an integrated framework to depict the evolution of the Moon and has important implications for the geologic study of other terrestrial planets.

Dynamic Effect of Landslides Triggered by Earthquake:A Case Study in Moxi Town of Luding County,China

The dynamic effect is a very important issue widely debated by scholars when studying the genetic and disaster-causing mechanisms of earthquake-triggered landslides.First,the dynamic effect mechanism and phenomena of earthquake-triggered landslides were summarized in this paper.Then,the primary types of dynamic effects were further used to interpret the Mogangling landslide in Moxi Town of Luding County,China.A field investigation,remote sensing,numerical calculation and theoretical analysis were carried out to illustrate the failure mechanism of slope rock masses affected by earthquakes.The interaction between seismic waves and slope rock masses and the induced dynamic effect of slope rock masses were primarily accounted for in the analysis.The slope topography,rock mass weathering and unloading characteristics,river erosion,regional seismogenic structure,and rock mass structure characteristics were also discussed.The results showed that the formation of the Mogangling landslide was mainly related to the high amplification effect of seismic acceleration and back slope effects,interface dynamic stress effects,and double-sided slope effects of seismic waves caused by the catastrophic Ms 7.75 Moxi Earthquake in 1786.The principles for the site and route selection of large-scale infrastructure in the planning stage and the scientific prevention of seismic geological disasters were proposed on the basis of the dynamic effect of earthquake-induced landslides.