Relationship between endogenous digitalis－like factor and fluid therapy in burn－blast combined injury

Fifty dogs were inflicted with burn-blast combined injury and divided into 5 groups. All the experimental animals began to receive various amounts of fluid and sodium slat replacement 2 h after injury. Serum level of endogenous digitalis-like factor (EDLF

Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors

Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.

Effects of leukemia inhibitory factor and basic fibroblast growth factor on free radicals and endogenous stem cell proliferation in a mouse model of cerebral infarction

The present study established a mouse model of cerebral infarction by middle cerebral artery occlusion, and monitored the effect of 25 tJg/kg leukemia inhibitory factor and （or） basic fibroblast growth factor administration 2 hours after model establishment. Results showed that following administration, the number of endogenous neural stem cells in the infarct area significantly increased, malondialdehyde content in brain tissue homogenates significantly decreased, nitric oxide content, glutathione peroxidase and superoxide dismutase activity significantly elevated, and mouse motor function significantly improved as confirmed by the rotarod and bar grab tests. In particular, the effect of leukemia inhibitory factor in combination with basic fibroblast growth factor was the most significant. Results indicate that leukemia inhibitory factor and basic fibroblast growth factor can improve the microenvironment after cerebral infarction by altering free radical levels, improving the quantity of endogenous neural stem cells, and promoting neurological function of mice with cerebral infarction.

Effects of leukemia inhibitory factor on endogenous neural stem cell proliferation and glycoprotein-130 expression in a mouse model of cerebral infarction

Leukemia inhibitory factor （LIF） has been shown to promote proliferation of endogenous neural stem cells. In this study, we treated mice with cerebral infarction using LIF to investigate whether the LIF receptor subunit glycoprotein （gp）130 is involved in neuroprotection. After LIF treatment, the motor function of model mice was significantly improved. Immunofluorescence histochemistry showed increased numbers of endogenous neural stem cells surrounding the infarct foci. Western blot analysis revealed that gp130 expression was significantly decreased surrounding the infarcted foci. Results demonstrated that LIF promoted the proliferation of endogenous neural stem cells by inhibiting gp130 protein expression.

Microbial spectrum and risk factors of endogenous endophthalmitis in a tertiary center of Northern China

AIM: To study the clinical features, microbial spectrum, associated factors and prognosis of endogenous endophthalmitis(EE) in a group of Chinese patients.METHODS: The medical records from 32 eyes of 29 patients diagnosed with EE in Peking Union Medical College Hospital from January 2009 to October 2019 were reviewed. RESULTS: The initial visual acuity(VA) of 30 eyes in this study was worse than 20/400. Twenty-three eyes were diagnosed with fungal endophthalmitis and nine with bacterial endophthalmitis. The most common fungal and bacterial isolates were Candida and Klebsiella pneumoniae, respectively. Several rare fungi and bacteria species were also isolated from our patients, including Cryptococcus, Paecilomyces, Brucella, and Bacillus licheniformis. The leading risk factor for EE was diabetes. The most common extraocular infection locus was genitourinary tract. Vitrectomy was performed on twenty-nine eyes. Eight eyes achieved final VA of 20/400 or better. EE caused by Candida had a better prognosis.CONCLUSION: The visual outcome of EE is based on pathogens and prompt intervention. Early vitrectomy and antimicrobial treatment are beneficial for EE.

Basic fibroblast growth factor increases the numbe of endogenous neural stem cells and inhibits the expression of amino methyl isoxazole propionic acid receptors in amyotrophic lateral sclerosis mice

This study aimed to investigate the number of amino methyl isoxazole propionic acid （AMPA） receptors and production of endogenous neural stem cells in the SOD1 G93AG1H transgenic mouse model of amyotrophic lateral sclerosis, at postnatal day 60 following administration of basic fibroblast growth factor （FGF-2）. A radioligand binding assay and immunohistochemistry were used to estimate the number of AMPA receptors and endogenous neural stem cells respectively. Results showed that the number of AMPA receptors and endogenous neural stem cells in the brain stem and sensorimotor cortex were significantly increased, while motor function was significantly decreased at postnatal days 90 and 120. After administration of FGF-2 into mice, numbers of endogenous neural stem cells increased, while expression of AMPA receptors decreased, whilst motor functions were recovered. At postnatal day 120, the number of AMPA receptors was negatively correlated with the number of endogenous neural stem cells in model mice and FGF-2-treated mice. Our experimental findings indicate that FGF-2 can inhibit AMPA receptors and increase the number of endogenous neural stem cells, thus repairing neural injury in amyotrophic lateral sclerosis mice.

The effect of endogenous transforming growth factor β_1 on the growth of bladder cancer cells in vitro

Objective To investigate the influence of endogenous transforming growth factor β1(TGFβ1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevTβ-AS, which carried antisense RNA of TGFβ1.was transfected to a bladder cancer cell line EJ. The proliferation and clone-formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGFβ1 antisense RNA was transferred into EJ cell and expressed efficiently. After the inhibition of target gene expression in EJ cells, the reduced growth and clone-formation rates were demonstrated, and the proliferative indexes were decreased by 12 % . The ratios of GO and G1 stage cells to June 2003 Vol12 No2 the antisense RNA-transfected EJ cells were increased, simultaneously,the ratio of S stage cells to the antisense RNA-transfected EJ cells ratios were decreased, compared with the control group. Conclusion The

Exploring prognostic potential of long noncoding RNAs in colorectal cancer based on a competing endogenous RNA network

BACKGROUND Colorectal cancer(CRC)is one of the most prevalent tumors worldwide.Recently,long noncoding RNAs(lncRNAs)have been shown to influence tumorigenesis and tumor progression by acting as competing endogenous RNAs(ceRNAs).It is difficult to extract prognostic lncRNAs and useful bioinformation from most ceRNA networks constructed previously.AIM To construct a prognostic related ceRNA regulatory network and lncRNA related signature based on risk score in CRC.METHODS RNA transcriptome profile and clinical information of 506 CRC patients were downloaded from the Cancer Genome Atlas database.R packages and Perl program were used for data processing.Cox regression analysis was used for prognostic model construction.Quantitative real-time polymerase chain reaction was used to detect the expression of lncRNAs.RESULTS A prognostic-related ceRNA network was constructed,including 9 lncRNAs,44 mRNAs,and 30 miRNAs.In addition,a four-lncRNA model was constructed using multivariate Cox regression analysis,which could be an independent prognostic model in CRC.The risk score for each patient was calculated,and the 506 patients were divided into high and low-risk groups(253 for each group)based on the median risk score.The results of the survival analysis showed that patients with a high-risk score had a poor survival rate.Furthermore,the predictive value of the four-lncRNA model was evaluated in GSE38832.Patient survival probabilities could be better predicted when combing the risk score and clinical features.Gene Set Enrichment Analysis results verified that a number of cancer-related signaling pathways were enriched with a high-risk score in CRC.Finally,we validated a novel lncRNA(LINC00488)using quantitative real-time polymerase chain reaction in 22 paired CRC patient tumor tissues compared to adjacent non-tumor tissues.CONCLUSION The four-lncRNA model could give better predictive value for CRC patients.Our understanding of the lncRNA-related ceRNA regulatory mechanism could provide a potential diagnostic indicator for CRC patients.

Endogenous versus exogenous cell replacement for Parkinson’s disease: where are we at and where are we going?

Parkinson’s disease is the second most common neurodegenerative disease and has currently no effective treatment,one that would be able to stop or reverse the loss of dopaminergic neurons in the substantia nigra pars compacta.In addition,Parkinson’s disease diagnosis is typically done when a significant percentage of the dopaminergic neurons is already lost.In neurodegenerative disorders,some therapeutic strategies could be effective only at inhibiting further degeneration;on the other hand,cell replacement therapies aim at replacing lost neurons,an approach that would be ideal for the treatment of Parkinson’s disease.Many cell replacement therapies have been tested since the 1970s in the field of Parkinson’s disease;however,there are still significant limitations prohibiting a successful clinical application.From the first fetal midbrain intrastriatal graft to the most recent conversion of astrocytes into dopaminergic neurons,we have gained equally,significant insights and questions still looking for an answer.This review aims to summarize the main milestones in cell replacement approaches against Parkinson’s disease.By focusing on achievements and failures,as well as on the additional research steps needed,we aim to provide perspective on how future cell replacement therapies treats Parkinson’s disease.

Temporal analysis of mRNA expression of endogenous TGFβ And its typeⅠ,typeⅡreceptor on burn wounds

Recent studies have shown that application of exogenous transforming growth factor?(TGF?)to a wound has a beneficial effect. However,little is known about the roles of endogenous TGF? and its receptors in the course of wound healing. In the present study, mRNA expression of endogenous TGF? and its type Ⅰ,type Ⅱreceptors on wounds of burned rats was observed by using dot blot hybridization.Thermal injury could induce expression of TGF ?, and its type Ⅰ,type Ⅱ receptor genes, and their expression appeared to be regulated. The expression was the strongest at 5 and 7 d postscalding when the repair of wounds was most active.The study suggests that TGF? plays an important role in epidermis regeneration, and that expression of TGF ? receptors is one of factors regulating wound healing. The expression discrepancy between TGF ? and its type Ⅰ,type Ⅱ receptor genes at the later stages of wound healing implies the presence of a well-controlled mechanism to limit excessive effect of endogenous TGF ? on repair cells.

Synthetic Study on Polyhydroxyestrogens Modeled for Inagami-Tamura Endogenous Digitalis-like Factor and Their Activity

1 Results Mammalian possess receptors for digitalis,cardiac glycosides,derived from plants.As one of the results from studies on a real endogenous ligand for the receptors,so called an endogenous digitalis-like factor (EDF) or substance (EDS),Inagami and Tamura isolated a promising candidate possessing a molecular weight,336.We elucidated several possible structures (Fig.1) for the factor and synthesized several modeled compounds,some of which exhibited remarkable constriction at higher concentrations t...

Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells

BACKGROUND Intervertebral disc(IVD) degeneration is a condition characterized by a reduction in the water and extracellular matrix content of the nucleus pulposus(NP) and is considered as one of the dominating contributing factors to low back pain. Recent evidence suggests that stromal cell-derived factor 1α(SDF-1α) and its receptor CX-C chemokine receptor type 4(CXCR4) direct the migration of stem cells associated with injury repair in different musculoskeletal tissues.AIM To investigate the effects of SDF-1α on recruitment and chondrogenic differentiation of nucleus pulposus-derived stem cells(NPSCs).METHODS We performed real-time RT-PCR and enzyme-linked immunosorbent assay to examine the expression of SDF-1α in nucleus pulposus cells after treatment with pro-inflammatory cytokines in vitro. An animal model of IVD degeneration was established using annular fibrosus puncture in rat coccygeal discs. Tissue samples were collected from normal control and degeneration groups.Differences in the expression of SDF-1α between the normal and degenerative IVDs were analyzed by immunohistochemistry. The migration capacity of NPSCs induced by SDF-1α was evaluated using wound healing and transwell migration assays. To determine the effect of SDF-1α on chondrogenic differentiation of NPSCs, we conducted cell micromass culture and examined the expression levels of Sox-9, aggrecan, and collagen II. Moreover, the roles of SDF-1/CXCR4 axis in the migration and chondrogenesis differentiation of NPSCs were analyzed by immunofluorescence, immunoblotting, and real-time RT-PCR.RESULTS SDF-1α was significantly upregulated in the native IVD cells cultured in vitro with pro-inflammatory cytokines, such as interleukin-1β and tumor necrosis factor-α, mimicking the degenerative settings. Immunohistochemical staining showed that the level of SDF-1α was also significantly higher in the degenerative group than in the normal group. SDF-1α enhanced the migration capacity of NPSCs in a dose-dependent manner. In addition, SDF-1α induced chondrogenic differentiation of NPSCs, as evidenced by the increased expression of chondrogenic markers using histological and immunoblotting analyses. Realtime RT-PCR, immunoblotting, and immunofluorescence showed that SDF-1αnot only increased CXCR4 expression but also stimulated translocation of CXCR4 from the cytoplasm to membrane, accompanied by cytoskeletal rearrangement.Furthermore, blocking CXCR4 with AMD3100 effectively suppressed the SDF-1α-induced migration and differentiation capacities of NPSCs.CONCLUSION These findings demonstrate that SDF-1α has the potential to enhance recruitment and chondrogenic differentiation of NPSCs via SDF-1/CXCR4 chemotaxis signals that contribute to IVD regeneration.

Analysis of Rural Development Factors: A Case Study of Hebei Province

On the basis of survey,drawing on economic growth theory,we analyze the main form of exogenous growth and regional applicability in Hebei Province,and point out that some exogenous driving forces are effective ways to accelerate rural construction,such as " the promotion of agriculture by industry" and " developing the rural areas with the aid of the urban areas". However,human capital,technology,incentive institutional arrangement,agricultural industry clusters and other endogenous growth factors are fundamental to sustainable development of rural construction,so it is necessary to make them play a fundamental role in increasing farmers' income and promoting agricultural development.

内外源因素对淀粉消化特性影响的研究进展

不同种类的淀粉其消化特性不同,且淀粉经加工处理后其消化特性也会发生改变。因此,通过探究影响淀粉消化特性的因素来调控淀粉的消化特性至关重要。基于此,该文对影响淀粉消化特性内源因素(淀粉种类、结构和非淀粉组分等)和外源因素(加工条件和非淀粉添加物等)进行综合梳理和分析,为研发消化特性不同的淀粉及淀粉类食品提供参考和依据。

贫血与非贫血孕妇Hb、VA、SF、Zn、IFA表达差异及相关性探究

目的探究贫血与非贫血孕妇血红蛋白(Hb)、维生素A(VA)、铁蛋白(SF)、锌(Zn)及内因子抗体(IFA)表达差异,并分析各指标表达与孕妇贫血的相关性。方法选取2022年8月至2023年4月我院收治的102例贫血孕妇为贫血组(Hb<110g/L),另选取同期102例非贫血孕妇为非贫血组,检测两组Hb、VA、SF、Zn、IFA表达水平,分析孕妇贫血的影响因素,将贫血组根据外周血Hb水平进行贫血程度划分[轻度(100g/L≤Hb<110g/L)、中度(70g/L≤Hb<99g/L)],比较不同贫血程度孕妇、不同孕期贫血孕妇Hb、VA、SF、Zn、IFA表达差异,分析Hb、VA、SF、Zn、IFA表达水平与贫血孕妇孕期、贫血程度的相关性,并分析Hb、VA、SF、Zn、IFA表达对中度贫血的预测价值。结果两组孕周、妊娠期合并症相比,差异有统计学意义(P<0.05);贫血组中度、轻度贫血孕妇Hb、VA、SF、Zn均低于非贫血组,IFA高于非贫血组(P<0.05),中度贫血孕妇Hb、VA、SF、Zn表达水平低于轻度贫血孕妇,IFA表达水平高于轻度贫血孕妇(P<0.05);不同孕期贫血孕妇Hb、VA、SF、Zn表达水平比较,孕晚期<孕中期<孕早期,IFA表达水平比较,孕早期<孕中期<孕晚期(P<0.05);Hb、VA、SF、Zn与贫血孕妇孕期、贫血程度呈负相关,IFA与贫血孕妇孕期、贫血程度呈正相关(P<0.05);孕周、妊娠期合并症、Hb、VA、SF、Zn、IFA是孕妇贫血的影响因素(P<0.05);Hb、VA、SF、Zn、IFA联合预测中度贫血的AUC为0.933,大于各参数单独预测(P<0.05)。结论贫血孕妇Hb、VA、SF、Zn、IFA表达明显异常,且Hb、VA、SF、Zn、IFA与贫血孕妇孕期、贫血程度联系密切,联合检测对预防孕妇中度贫血具有重要价值。

异龙湖水质时空演变特征及其驱动力分析

异龙湖是云南九大高原湖泊中流域面积最小、湖泊水量最少、水质污染程度相对最重、最易受人类活动干扰和近年来唯一干涸过的高原浅水型湖泊,受自然环境条件和人类活动双重驱动影响,近年来异龙湖水质演变过程十分复杂、水质污染问题日益突出。本文以湖泊水动力与水质模型为主要技术手段,并结合湖流原位观测,深入研究了异龙湖的水动力特性及入湖污染物的迁移扩散规律,初步揭示了异龙湖水质的时空演变特征及其成因。研究表明,水资源总量不足是影响异龙湖水质年际波动变化并逐步向封闭性湖泊转变的最主要因子,不利的湖流形态与不同特征污染物的来源组成是驱动异龙湖水质时空分布差异性的关键因素,随降雨径流输入的外源负荷、湖区浮游植物异常增殖合成的大量有机物和湖泊底泥释放的大量内源负荷是异龙湖2021年水质变差与快速返劣的重要驱动因素,因此,增加流域内清洁水资源量和入出湖水量、加快湖泊内源与陆域污染物输入治理、强化对雨季主要入湖河流水质浓度的管控,是实现近期湖区水质快速脱劣并持续性改善的关键。

龙陵县新寨-茅草园稀土矿特征及控矿条件浅析

龙陵新寨-茅草园地区花岗岩风化壳离子吸附型稀土矿床受内生地质和表生地质条件双重控制,主要赋存于白垩纪花岗岩体全风化层中下部及强风化层上部,V 1-1矿体分布面积38.59km 2,单工程控制矿体品位(SREO)0.035%~0.128%,平均0.047%;V 1-2矿体分布面积16.85 km 2,单工程控制矿体品位(SREO)0.035%~0.110%,平均0.048%,达大型远景规模,具有较好的勘查开发前景。

行动者网络视角下乡村内生发展的影响因素与路径研究——基于福建省32个案例的模糊集定性比较分析

当前我国乡村振兴战略实施取得明显成效,但乡村发展内生动力和能力不足的问题凸显。如何促进乡村内生发展成为政府决策部门和学术界共同关注的热点。文章结合行动者网络理论和新内生发展理论构建理论分析框架,运用模糊集定性比较分析法对福建省32个村社案例进行分析,研究乡村内生发展的影响因素和路径组合。研究结果表明:(1)乡村内生发展受到地方内部与外部力量、人类行动者与非人类行动者的共同作用。产业基础、政府支持变量的一致性水平超过0.90,是乡村内生发展的必要条件;党建引领、精英带动、资源禀赋、技术支持变量的一致性水平介于0.80~0.90之间,是乡村内生发展的充分条件。(2)各因素形成乡村内生发展的七条路径,根据因素匹配之间的差异,可归纳为内部主导型、外部驱动型、内外联动型三种模式。文章拓展了乡村内生发展的研究视角,为寻找乡村内生发展路径提供了经验参考。基于上述研究结果,提出推动乡村内生发展的政策启示:(1)立足整体,人类行动者和非人类行动者联动匹配激发乡村内生发展动力;(2)因地制宜,内外部要素互相作用提升乡村内生发展能力;(3)优化政府支持引导,打造多产融合发展格局,强化乡村内生发展的核心要件。

菊苣肉质根膨大与其生理及基因表达变化初步研究

初步探究菊苣肉质根膨大发育进程的农艺性状、生理指标和基因表达变化,以期进一步探讨肉质根发育的生理和分子调控机理。以盆栽根用菊苣‘Hera’和叶用菊苣‘将军’为材料,获取8个不同发育时期的肉质根样品,对糖和内源激素进行定量分析,并对转录因子基因进行qPCR表达量分析。结果表明,根用菊苣根径、根干质量、根冠比和根库活性均大于叶用菊苣。‘Hera’肉质根果糖、葡萄糖和蔗糖含量分别在S2、S3和S4时期最高,且葡萄糖、果糖含量在各时期均高于‘将军’。‘Hera’肉质根生长素IAA、细胞分裂素CTK和赤霉素GA含量均呈现膨大前期高、中期低、后期较高的态势,转录因子基因CiMYB73、CiERF13、CiNAC017、CibZIP53均在S2时期(播种后38 d)呈现高表达。根用菊苣与叶用菊苣农艺性状差异显著,糖和激素可能协同作用于肉质根的膨大发育,播种后38 d可能为菊苣肉质根膨大发育的关键时期。研究结果对后续菊苣肉质根的遗传调控研究、基因工程改良等有一定的参考价值。

薄层砂岩边底水油藏空气辅助内源微生物驱油技术

针对边底水油藏“双高阶段”零散分布剩余油如何有效动用、低成本高效微生物驱油体系如何研发、微生物驱效果评价及配套技术攻关等难题,建立微生物驱筛选图版并评价了油藏微生物驱的适应性;利用微生物分子生态学技术全面解析了油藏微生物群落结构,筛选了乳化效果好的采油功能菌株H3;通过对主要营养成分碳源、氮源、磷源的筛选,开展单因素实验和正交实验,确定了可实现原油乳化分散和抑制硫酸盐还原菌生长的复合粉激活体系;研究了底水油层“船底形”剩余油富集特征,形成了“微生物乳化+空气提效+稠化剂封堵”提高采收率驱油模式,明确了提高采收率幅度与注入菌体浓度、注入孔隙体积倍数正相关。将研究成果应用于准噶尔盆地陆梁油田薄层砂岩边底水油藏,实施“4注20采”微生物驱提高采收率矿场先导试验,注入微生物激活剂0.13倍孔隙体积,2017至2023年阶段增油5.6×104 t,采收率提高4.0个百分点,增油效果好的井主要分布在总菌浓度高、石油地质储量大、构造高部位、油层厚度大、底水薄、采出程度较高的区域。研究成果对丰富微生物驱提高采收率技术序列具有借鉴作用。