期刊文献+
共找到482篇文章
< 1 2 25 >
每页显示 20 50 100
Expression of platelet endothelial cell adhesion molecule-1 between pancreatic microcirculation and peripheral circulation in rats with acute edematous pancreatitis 被引量:6
1
作者 Hong-Kai Gao Zong-Guang Zhou +2 位作者 You-Qin Chen Fang-Hai Han Cun Wang the Department of General Surgery and Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2003年第3期463-466,共4页
OBJECTIVE: To study the ehanges of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation anti pancreatic microcirculation in rats with acut... OBJECTIVE: To study the ehanges of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation anti pancreatic microcirculation in rats with acute edematous pancreatitis (AEP). METHODS: The model of AEP was established with 50 Wistar rats, and the changes of PECAM-1 expression on PMNs from the splenic vein and inferior vena cava were determined by flow cytometry. RESULTS: PECAM-I expression on PMNs showed no significant difference between pancreatic microcirculation and peripheral circulation at AEP2h and AEP4h time points. From the AEP4h to the AEP8h time point, PECAM-1 expression in peripheral circulation was up-regulated, but PECAM-1 expression in pancreatic microcirculation was down-regulated. PECAM-1 expression had a significant difference between pancreatic microcirculation and peripheral circulation at the AEP8h time point (P<0.05). CONCLUSION: PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in AEP. 展开更多
关键词 acute edematous pancreatitis peripheral circulation pancretic microcirculation polymorphonuclear leukocyte platelet endothelial cell adhesion molecules-1
下载PDF
Effects of TCMP-1 on the changes of platelet endothelial cell adhesion molecule-1 expression in acute edematous pancreatitis 被引量:4
2
作者 Hong-Kai Gao, Zong-Guang Zhou, Tao He, You-Qin Chen, Fang-Hai Han and Cun Wang Chengdu, ChinaDepartment of General Surgery & Division of Diges- tive Surgery, West China Hospital, Sichuan University, Chengdu 610041 , China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2004年第2期311-315,共5页
BACKGROUND: Traditional Chinese medicine is a potent agent in the management of clinical and experimental acute pancreatitis (AP), but the molecular mechanism of its the- rapeutic action is unclear. Numerous experimen... BACKGROUND: Traditional Chinese medicine is a potent agent in the management of clinical and experimental acute pancreatitis (AP), but the molecular mechanism of its the- rapeutic action is unclear. Numerous experimental and clinical studies have shown that platelet endothelial cell ad- hesion molecule-1 (PECAM-1) is pivotal to leukocyte re- cruitment, which results in microcirculatory injury during inflammation, but its role in acute pancreatitis is poorly un- derstood. We investigated the effects of a compound of tra- ditional Chinese medicine pancreatitis-1 (TCMP-1) on the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in acute edematous pancreatitis (AEP). METHODS: The model of acute pancreatitis was estab- lished by subcutaneous injection of caerulein, and TCMP-1 treated groups were given TCMP-1 by catheterization from mouth to stomach (20 ml/kg) immediately after first time subcutaneous injection of caerulein. The changes of expres- sion of PECAM-1 on leukocytes from the blood of the splenic vein and inferior vena cava were determined by flow cytometry. RESULTS: In the AEP group, expression of PECAM-1 on PMNs was not significantly different between pancreatic microcirculation and systemic circulation at AEP2h and AEP4h time point. Then from AEP4h time point to AEP8h time point, expression of PECAM-1 was up-regulated in systemic circulation while it was down-regulated in pancre- atic microcirculation and was significantly different be- tween pancreatic microcirculation and systemic circulation at AEP8h time point (P<0.05). In the TCMP-1 treated group, compared with the AEP group, expression of PE-CAM-1 on PMNs decreased in different levels between pan- creatic microcirculation and systemic circulation and was of significant difference at AEP8h time point (P <0.05). CONCLUSION: Inhibition of PECAM-1 expression on PMNs may prevent PMNs from transmigration through the endo- thelium and may be one of the treatment mechanisms of TCMP-1 decoction on AEP. 展开更多
关键词 acute pancreatitis platelet endothelial cell adhesion molecule-1 Chinese medicine
下载PDF
The relationship between platelet endothelial cell adhesion molecule-1 and paraquat-induced lung injury in rabbits 被引量:7
3
作者 Jing Shi Chun-lin Hu +2 位作者 Yu-feng Gao Xiao-xing Liao Hope Xu 《World Journal of Emergency Medicine》 CAS 2012年第1期60-64,共5页
BACKGROUND:Platelet endothelial cell adhesion molecule-1(PECAM-1),also known as CD31,is mainly distributed in vascular endothelial cells.Studies have shown that PECAM-1 is a very significant indicator of angiogenesis,... BACKGROUND:Platelet endothelial cell adhesion molecule-1(PECAM-1),also known as CD31,is mainly distributed in vascular endothelial cells.Studies have shown that PECAM-1 is a very significant indicator of angiogenesis,and has been used as an indicator for vascular endothelial cells.The present study aimed to explore the relationship between the expression of PECAM-1 and the degree of acute lung injury(ALI) and fibrosis in paraquat(PQ) induced lung injury in rabbits.METHODS:Thirty-six adult New Zealand rabbits were randomly divided into three groups(12rabbits in each group) according to PQ dosage:8 mg/kg(group A),16 mg/kg(group B),and 32 mg/kg(group C).After PQ infusion,the rabbits were monitored for 7 days and then euthanized.The lungs were removed for histological evaluation.Masson staining was used to determine the degree of lung fibrosis(LF),and semi-quantitative immune-histochemistry analysis to determine the expression of PECAM-1.Pearson's product-moment correlation analysis was performed to evaluate the relationship between the expression of PECAM-1 and the extent of lung injuries expressed by ALI score and degree of LF.RESULTS:Rabbits in the three groups showed apparent poisoning.The rabbits survived longer in group A than in groups B and C(6.47±0.99 days vs.6.09±1.04 days vs.4.77±2.04 days)(P<0.05).ALI score was lower in group A than in groups B and C(8.33±1.03 vs.9.83±1.17 vs.11.50±1.38)(P<0.05),and there was statistically significant difference between group B and group C(P=0.03).LF was slighter in group A than in groups B and C(31.09%±2.05%vs.34.37%±1.62%vs.36.54%±0.44%)(P<0.05),and there was statistically significant difference between group B and group C(P=0.026).The PEACAM-1 expression was higher in group A than in groups B and C(20.31%±0.70%vs.19.34%±0.68%vs.18.37%±0.46%)(P<0.05),and there was statistically significant difference between group B and group C(P=0.017).Pearson's correlation analysis showed that the expression of PECAM-1 was negatively correlated to both ALI score(Coe=-0.732,P=0.001)and degree of LF(Coe=-0.779,P<0.001).CONCLUSIONS:The PECAM-1 expression significantly decreases in New Zealand rabbits after PQ poisoning,and the decrease is dose-dependent.The PECAM-1 expression is negatively correlated with ALI score and LF,showing a significant role in the development of lung injuries induced by PQ. 展开更多
关键词 Platelet endothelial cell adhesion molecule-1 PARAQUAT Acute lung injury Lung fibrosis
下载PDF
Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis 被引量:2
4
作者 Hong-KaiGao Zong-GuangZhou +5 位作者 Fang-HaiHan You-QinChert Wen-WeiYan TaoHe CunWang ZhaoWang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第5期661-664,共4页
AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induce... AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5 μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1mRNA expression was seen from 4 to 8 h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21% ), while in pancreatic microcirculation,expression decreased from 2 h and reached the lowest level at 6 h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%,0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral circulation and pancreatic microcirculation (1.00±0.21% vs0.66±0.20%, P<0.05).Meanwhile,the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1expression may improve the pathological change of AEP. 展开更多
关键词 Pancreatitis Platelet endothelial cell adhesion molecule-1 Microcirculation CERULEIN
下载PDF
Association of G+1688A Polymorphism of Platelet Endothelial Cell Adhesion Molecule-1 Gene with Myocardial Infarction in the Chinese Han Population 被引量:1
5
作者 杨颖 程龙献 +3 位作者 Ripen Nsenga 何美安 常智堂 邬堂春 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第5期520-523,共4页
In order to investigate the association of G+1688A (Ser563Asn) polymorphism of platelet endothelial cell adhesion molecule-1 (PECAM-1) gene with myocardial infarction (MI) in the Chinese Han population, the G+... In order to investigate the association of G+1688A (Ser563Asn) polymorphism of platelet endothelial cell adhesion molecule-1 (PECAM-1) gene with myocardial infarction (MI) in the Chinese Han population, the G+1688A polymorphism in PECAM-1 gene was detected by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method among 502 subjects, including 218 patients with MI and 284 controls. The results showed that there was significant difference in AA frequencies of genotype G+1688A polymorphism between case and control groups (39% vs 24%, P〈0.001). A similar trend was observed on the allele frequencies (A/G: 62% vs 49%, P〈0.001). Among the subjects with high serum total cholesterol level or high systolic blood pressure level, the variant AA genotype was associated with high risk of MI (adjusted OR, 2.13; 95% CI, 1.08 -4.41 and adjusted OR, 2.53; 95%CI, 1.63-3.63). The single nucleotide polymorphism (SNP) at position +1688 in the exon 8 of PECAM-1 gene was associated with MI and the allele A might be a risk factor for MI in the Chinese Han population. 展开更多
关键词 platelet endothelial adhesion molecule-1 single nucleotide polymorphism myocardial infarction polymerase chain reaction-restriction fragment-length polymorphism
下载PDF
Adhesion of different cell cycle human hepatoma cells to endothelial cells and roles of integrin β_1 被引量:4
6
作者 Guan-BinSong JianQin QingLuo Xiao-DongShen Run-BinYan Shao-XiCai 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第2期212-215,共4页
AIM: To investigate the adhesive mechanical properties of different cell cycle human hepatoma cells (SMMC-7721) to human umbilical vein endothelial cells (ECV-304), expression of adhesive molecule integrinβ1 in SMMC-... AIM: To investigate the adhesive mechanical properties of different cell cycle human hepatoma cells (SMMC-7721) to human umbilical vein endothelial cells (ECV-304), expression of adhesive molecule integrinβ1 in SMMC-7721 cells and its contribution to this adhesive course. METHODS: Adhesive force of SMMC-7721 cells to endothelial cells was measured using micropipette aspiration technique. Synchronous G1 and S phase SMMC-7721 cells were achieved by thymine-2-deoxyriboside and colchicines sequential blockage method and double thymine-2-deoxyriboside blockage method, respectively. Synchronous rates of SMMC-7721 cells and expression of integrinβ1 in SMMC-7721 cells were detected by flow cytometer. RESULTS: The percentage of cell cycle phases of general SMMC-7721 cells was 11.01% in G2/M phases, 53.51% in G0/G1 phase, and 35.48% in S phase. The synchronous rates of G1 and S phase SMMC-7721 cells amounted to 74.09% and 98.29%, respectively. The adhesive force of SMMC-7721 cells to endothelial cells changed with the variations of adhesive time and presented behavior characteristics of adhesion and de-adhesion. S phase SMMC-7721 cells had higher adhesive forces than d phase cells [(307.65±92.10)×10-10N vs(195.42±60.72)×10-10N, P<0.01]. The expressive fluorescent intensity of integrinβ1 in G1 phase SMMC-7721 cells was depressed more significantly than the values of S phase and general SMMC-7721cells. The contribution of adhesive integrinβ1 was about 53% in this adhesive course. CONCLUSION: SMMC-7721 cells can be synchronized preferably in d and S phases with thymine-2-deoxyriboside and colchicines. The adhesive molecule integrinβ1 expresses a high level in SMMC-7721 cells and shows differences in various cell cycles, suggesting integrin β1 plays an important role in adhesion to endothelial cells. The change of adhesive forces in different cell cycle SMMC-7721 cells indicates that S phase cells play predominant roles possibly while they interact with endothelial cells. 展开更多
关键词 HEPATOMA cell cycle Integrin β1 endothelial cells cell adhesion
下载PDF
血清VCAM-1、PECAM-1水平与MMSE评分联合检测对老年全髋关节置换术患者术后谵妄的预测价值
7
作者 李繁 黎仕焕 谢爽 《天津医药》 CAS 2024年第10期1046-1051,共6页
目的探讨术前血清血管细胞黏附分子-1(VCAM-1)、血小板内皮细胞黏附分子-1(PECAM-1)水平与简易精神状态量表(MMSE)评分联合检测对老年全髋关节置换术(THA)患者术后谵妄(POD)的预测价值。方法选择住院并行手术治疗的髋部骨折老年患者200... 目的探讨术前血清血管细胞黏附分子-1(VCAM-1)、血小板内皮细胞黏附分子-1(PECAM-1)水平与简易精神状态量表(MMSE)评分联合检测对老年全髋关节置换术(THA)患者术后谵妄(POD)的预测价值。方法选择住院并行手术治疗的髋部骨折老年患者200例作为研究对象,并根据术后3 d内是否发生POD分为POD组(44例)和非POD组(156例)。收集2组患者的临床资料,术前采用MMSE评估患者的认知状况,并检测术前、术后第1天和第3天血清VCAM-1、PECAM-1水平。对比2组患者上述指标的差异,并分析术前血清VCAM-1、PECAM-1水平与MMSE评分的相关性。应用多因素Logistic回归分析老年THA患者发生POD的影响因素。构建受试者工作特征(ROC)曲线评估术前血清VCAM-1水平、PECAM-1水平、MMSE评分单独及联合检测对老年THA患者并发POD的预测价值。结果POD组年龄、医院焦虑抑郁量表评分、术中低血压发生率、术后住院时间明显高于或长于非POD组,MMSE评分低于非POD组(P<0.05)。POD组术前、术后第1天和术后第3天血清VCAM-1、PECAM-1水平升高,且高于非POD组(P<0.05)。老年THA患者术前血清VCAM-1、PECAM-1水平分别与MMSE评分呈负相关(r分别为-0.390、-0.501,均P<0.01)。术前血清VCAM-1和PECAM-1水平升高以及术后住院时间延长为老年THA患者发生POD的独立危险因素,MMSE评分升高为独立保护因素(P<0.05)。术前血清VCAM-1(AUC=0.793,95%CI:0.730~0.847)、PECAM-1(AUC=0.799,95%CI:0.736~0.852)及MMSE评分(AUC=0.805,95%CI:0.744~0.858)对THA患者发生POD均有较高的预测价值,三项指标联合预测的效能更高。结论血清VCAM-1、PECAM-1水平升高与老年THA患者认知功能受损有关,且均为老年THA患者发生POD的独立预测因子,术前检测以上指标可能对POD的早期防治具有重要价值。 展开更多
关键词 关节成形术 置换 谵妄 术后认知并发症 血管细胞黏附分子1 血小板内皮细胞黏附分子1 老年人 简易智能状态量表
下载PDF
血TG/HDL-C、PECAM-1、IL-19联合检测预测短暂性脑缺血发作患者近期发生急性脑梗死的价值
8
作者 杜迎春 高延伦 +1 位作者 侯兵兵 姚鸣鸣 《海南医学》 CAS 2024年第21期3075-3080,共6页
目的探讨联合检测血甘油三酯(TG)/高密度脂蛋白胆固醇(HDL-C)、血小板内皮细胞黏附分子1(PECAM-1)、白介素-19(IL-19)预测短暂性脑缺血发作(TIA)患者近期发生急性脑梗死(ACI)的价值。方法选取2021年2月至2023年7月平顶山市第一人民医院... 目的探讨联合检测血甘油三酯(TG)/高密度脂蛋白胆固醇(HDL-C)、血小板内皮细胞黏附分子1(PECAM-1)、白介素-19(IL-19)预测短暂性脑缺血发作(TIA)患者近期发生急性脑梗死(ACI)的价值。方法选取2021年2月至2023年7月平顶山市第一人民医院收治的353例TIA患者进行前瞻性研究,根据6个月内是否发生ACI分为ACI组(n=46)和无ACI组(n=307)。比较两组患者的基线资料及治疗前的血TG/HDL-C、PECAM-1、IL-19水平,采用多因素Logistic回归分析TG/HDL-C、PECAM-1、IL-19对TIA近期发生ACI的影响,采用受试者工作特征(ROC)曲线分析TG/HDL-C、PECAM-1、IL-19、年龄、血压、临床症状、糖尿病和持续的时间评分(ABCD3-I)预测TIA患者近期发生ACI的效能,DeLong检验比较TG/HDL-C+PECAM-1+IL-19与ABCD3-I评分的ROC曲线下面积(AUC)。结果ACI组患者的TIA发作次数、颈动脉和颅内动脉有不稳定斑块患者占比、ABCD3-I评分分别为(4.82±1.52)次、45.65%(21/46)、23.91%(11/46)、(6.86±1.24)分,明显高于无ACI组患者的(2.00±0.61)次、29.32%(90/307)、13.68%(42/307)、(4.64±0.95)分,差异均有统计学意义(P<0.05)。ACI组患者的TG/HDL-C、PECAM-1、IL-19分别为1.23±0.12、(11.42±2.36)mg/L、(82.44±17.35)ng/L,明显高于无ACI组患者的1.08±0.10、(8.99±2.64)mg/L、(67.28±19.00)ng/L,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,校正了TIA发作次数、颈动脉有不稳定斑块、颅内动脉狭窄情况、ABCD3-I评分后,TG/HDL-C、PECAM-1、IL-19仍是TIA近期发生ACI独立相关危险因素(P<0.05)。ROC分析结果显示,TG/HDL-C、PECAM-1、IL-19预测TIA近期发生ACI的AUC分别为0.812、0.792、0.798;TG/HDL-C、PECAM-1联合IL-19的AUC为0.930,大于ABCD3-I评分的0.830(P<0.05);三者联合的预测敏感度为93.48%,特异度为81.43%。结论血TG/HDL-C、PECAM-1、IL-19是TIA近期发生ACI的独立相关因素,与ABCD3-I评分相比,联合检测三者预测TIA近期发生ACI风险的价值较高,能为临床防治ACI提供一定的参考信息。 展开更多
关键词 甘油三酯/高密度脂蛋白胆固醇 血小板内皮细胞黏附分子1 白介素-19 短暂性脑缺血发作 急性脑梗死
下载PDF
Hypoxia Inhibits Proliferation of Human Dermal Lymphatic Endothelial Cells via Downregulation of Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 Expression
9
作者 Qi XIE Tong-yao SHANG +5 位作者 Shuo FENG Ru-cai ZHAN Jing LIANG Meng-ge FAN Liang ZHANG Ju LIU 《Current Medical Science》 SCIE CAS 2021年第6期1192-1197,共6页
Objective:Lymphatic endothelial cell(LEC)proliferation is essential for lymphangiogenesis.Hypoxia induces lymphangiogenesis,but it directly inhibits LEC proliferation and the underlying mechanisms have not been fully ... Objective:Lymphatic endothelial cell(LEC)proliferation is essential for lymphangiogenesis.Hypoxia induces lymphangiogenesis,but it directly inhibits LEC proliferation and the underlying mechanisms have not been fully understood.The aim of this study was to investigate the role of carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1)in hypoxia-repressed LEC proliferation.Methods:Human dermal lymphatic endothelial cells(HDLECs)were cultured under normoxic or hypoxic conditions,and cell proliferation was determined using MTT or CCK-8 assays.CEACAM1 expression was silenced by siRNA transfection.Activation of mitogen-activated protein kinases(MAPKs)was examined by Western blotting and blocked by specific inhibitors.Results:Under hypoxia,HDLECs proliferation was suppressed and CEACAM1 expression was downregulated.Silence of CEACAM1 in normoxia inhibited HDLECs proliferation and did not further decrease proliferation in HDLECs in response to hypoxia,suggesting that CEACAM1 may mediate hypoxia-induced inhibition of HDLECs proliferation.In addition,silence of CEACAM1 increased phosphorylation of MAPK molecules:extracellular signal-regulated kinase(ERK),p38 MAPK and Jun N-terminal kinase(JNK)in HDLECs.However,only inhibition of the JNK pathway rescued the reduction of HDLEC proliferation induced by CEACAM1 silence.Conclusion:Our results suggested that hypoxia downregulates CEACAM1 expression by activation of the JNK pathway,leading to inhibition of HDLEC proliferation.These findings may help to understand the mechanisms of LEC-specific response to hypoxia and develop novel therapies for pathological lymphangiogenesis. 展开更多
关键词 lymphatic endothelial cell HYPOXIA PROLIFERATION carcinoembryonic antigen-related cell adhesion molecule 1 Jun N-terminal kinase pathway
下载PDF
The law of anti-VCAM-1 targeted microbubbles adhesion to activated endothelial cells under controlled shear flow
10
作者 Lie Zhang,Hong Yang,Yiyao Liu(Department of Biophysics,School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,Sichuan,China) 《医用生物力学》 EI CAS CSCD 2009年第S1期11-11,共1页
Microbubbles can enhance the detection in noninvasive ultrasound imaging.Recently,targeted microbubbles have been developed to selectively adhere to specific and overexpressed p molecules in endothelial cells in some ... Microbubbles can enhance the detection in noninvasive ultrasound imaging.Recently,targeted microbubbles have been developed to selectively adhere to specific and overexpressed p molecules in endothelial cells in some pathologic conditions.However,the law of 展开更多
关键词 HUVECs The law of anti-VCAM-1 targeted microbubbles adhesion to activated endothelial cells under controlled shear flow
下载PDF
ACI患者血清APN、Lp-PLA2、PECAM-1水平与出院后一年认知功能障碍的相关性探讨
11
作者 于媛媛 代建霞 刘媛 《脑与神经疾病杂志》 CAS 2024年第6期331-336,共6页
目的 探讨急性脑梗死(ACI)患者出院时血清脂联素(APN)、脂蛋白相关磷脂酶A2(Lp-PLA2)、血小板内皮细胞黏附分子-1 (PECAM-1)水平与长期认知功能障碍(PSCI)的相关性,为临床预测PSCI提供相关标志物。方法 选取新疆维吾尔自治区人民医院202... 目的 探讨急性脑梗死(ACI)患者出院时血清脂联素(APN)、脂蛋白相关磷脂酶A2(Lp-PLA2)、血小板内皮细胞黏附分子-1 (PECAM-1)水平与长期认知功能障碍(PSCI)的相关性,为临床预测PSCI提供相关标志物。方法 选取新疆维吾尔自治区人民医院2020年8月至2022年1月ACI患者108例,根据出院后1年时是否存在PSCI,分为认知正常组(77例)与认知障碍组(31例)。比较两组临床基本资料及入院时、出院时血清APN、Lp-PLA2、PECAM-1水平,分析血清APN、Lp-PLA2、PECAM-1水平与长期PSCI的相关性。结果 认知障碍组出院后1年简易精神状态评价量表(MMSE)评分为(22.13±2.06)分,低于认知正常组(29.14±0.31)分(P<0.05);两组年龄、神经功能缺损程度、合并糖尿病、糖化血红蛋白(HbAlc)、同型半胱氨酸(Hcy)水平差异有统计学意义(P<0.05);认知障碍组出院时血清APN水平低于认知正常组,Lp-PLA2、PECAM-1水平高于认知正常组(P<0.05);出院时血清APN水平与出院后1年MMSE评分呈正相关,Lp-PLA2、PECAM-1水平与出院后1年MMSE评分呈负相关(r=0.727、-0.667、-0.750,均P<0.05);Logistic回归分析,将其他因素校正前后,出院时血清APN、Lp-PLA2、PECAM-1水平均与ACI患者长期PSCI独立相关(P<0.05);出院时血清APN、LpPLA2、PECAM-1水平预测ACI患者长期PSCI的AUC分别为0.783 (95%CI:0.693~0.857)、0.736 (95%CI:0.643~0.817)、0.827 (95%CI:0.743~0.893),联合预测ACI患者长期PSCI的AUC为0.936 (95%CI:0.872~0.974),优于3者单独预测。结论 ACI患者出院时血清APN、Lp-PLA2、PECAM-1水平与长期PSCI独立相关,可作为临床早期预测指标,且联合预测价值可靠。 展开更多
关键词 急性脑梗死 脂联素 脂蛋白相关磷脂酶A2 血小板内皮细胞黏附分子-1认知功能障碍 相关性
下载PDF
血清PECAM-1、PLGF水平联合子宫动脉多普勒超声参数检测在妊娠高血压患者不良妊娠结局中的预测价值
12
作者 朱会玲 王晓莹 李虹瑾 《航空航天医学杂志》 2024年第8期901-904,共4页
目的探究子宫动脉多普勒超声参数、血清血小板内皮细胞黏附分子-1(PECAM-1)、胎盘生长因子(PLGF)联合检测对妊娠高血压(HDCP)患者不良妊娠结局的预测价值。方法选取2022年09月-2023年08月于我院就诊的65例HDCP患者作为观察组,同期选取6... 目的探究子宫动脉多普勒超声参数、血清血小板内皮细胞黏附分子-1(PECAM-1)、胎盘生长因子(PLGF)联合检测对妊娠高血压(HDCP)患者不良妊娠结局的预测价值。方法选取2022年09月-2023年08月于我院就诊的65例HDCP患者作为观察组,同期选取65例健康产检者作为对照组进行研究,比较2组及不同程度HDCP患者子宫动脉多普勒超声参数[脐动脉搏动指数(PI)、阻力指数(RI)、血流速度峰谷比(S/D)]及血清PECAM-1、PLGF水平,通过比较不同妊娠结局的子宫动脉多普勒超声参数及血清PECAM-1、PLGF水平,绘制受试者工作特性(ROC)曲线分析预测价值。结果研究组RI、PI、S/D均高于对照组,且不良妊娠结局者RI、PI、S/D高于良好妊娠者(P<0.05);研究组及不良妊娠结局者血清PECAM-1、PLGF水平均明显低于对照组及良好妊娠结局者(P<0.05);HDCP组血清PECAM-1、PLGF水平高于轻度子痫前期组,PI、RI、S/D低于轻度子痫前期组;重度子痫前期组血清PLGF、PECAM-1水平低于轻度子痫前期组,RI、PI、S/D高于轻度子痫前期组(P<0.05);血清PECAM-1、PLGF水平联合多普勒超声参数(子宫动脉)预测HDCP患者不良妊娠结局的AUC为0.916,95%CI为0.820~0.970,均高于单独诊断。结论血清PECAM-1、PLGF水平、子宫动脉多普勒超声参数联合检测在预测HDCP疾病不良妊娠结局中具有较高的预测价值,临床意义较高。 展开更多
关键词 子宫动脉多普勒超声参数 血小板内皮细胞黏附分子-1 胎盘生长因子 妊娠高血压 不良妊娠结局
下载PDF
脑出血患者脑组织中EPCR、PECAM-1表达水平与疾病严重程度的关系
13
作者 金俊 唐森 《中国医学工程》 2024年第10期25-29,共5页
目的探究脑出血患者脑组织中内皮细胞蛋白C受体(EPCR)、血小板内皮细胞黏附分子1(PECAM-1)表达水平与疾病严重程度的关系。方法选取2019年3月至2020年8月于驻马店市中心医院就诊的脑出血患者100例为研究对象,收集临近血肿0.5 cm脑组织(... 目的探究脑出血患者脑组织中内皮细胞蛋白C受体(EPCR)、血小板内皮细胞黏附分子1(PECAM-1)表达水平与疾病严重程度的关系。方法选取2019年3月至2020年8月于驻马店市中心医院就诊的脑出血患者100例为研究对象,收集临近血肿0.5 cm脑组织(脑出血组织标本,脑出血组)和远隔血肿位置的脑组织(对照组织标本,对照组)。根据出血量的不同将患者分为少量组(n=35)、中量组(n=44)、大量组(n=21);根据病情严重程度将患者分为轻型组(n=40)、中型组(n=40)例、重型组(n=20)。采用免疫组织化学法检测组织中EPCR、PECAM-1表达水平;Spearman法分析脑出血组织EPCR、PECAM-1阳性表达率与美国国立卫生研究院卒中量表(NIHSS)评分的相关性;Logistic回归分析脑出血发生的影响因素。结果免疫组织化学染色结果显示,EPCR在脑出血组的阳性表达率高于对照组,而PECAM-1在脑出血组的阳性表达率低于对照组(P<0.05);少量组、中量组、大量组脑出血组织EPCR阳性表达率依次升高,PECAM-1阳性表达率依次降低(P<0.05);轻型组、中型组、重型组脑出血组织EPCR阳性表达率依次升高,PECAM-1阳性表达率依次降低(P<0.05);脑出血组织EPCR阳性表达率与患者NIHSS评分呈正相关(r=0.416,P<0.001),PECAM-1阳性表达率与患者NIHSS评分呈负相关(r=-0.337,P<0.001);EPCR是影响脑出血发生的危险因素,而PECAM-1是影响脑出血发生的保护因素(P<0.05)。结论脑出血患者脑组织中EPCR呈高表达,PECAM-1呈低表达,均与病情严重程度和出血量有关,可能作为评估脑出血患者病情的潜在标志物。 展开更多
关键词 内皮细胞蛋白C受体 血小板内皮细胞黏附分子1 脑出血 疾病严重程度
下载PDF
PECAM-1、心室重构与急性心肌梗死冠脉介入术后合并重度心力衰竭的相关性及预后预测
14
作者 陈荣红 《中华养生保健》 2024年第9期17-20,共4页
目的探讨血小板内皮细胞黏附分子-1(PECAM-1)、心室重构与急性心肌梗死冠脉介入术后合并重度心力衰竭的相关性及预后预测价值。方法选取2020年5月—2023年5月内蒙古包钢医院收治的80例急性心肌梗死冠脉介入术后合并心力衰竭患者作为研... 目的探讨血小板内皮细胞黏附分子-1(PECAM-1)、心室重构与急性心肌梗死冠脉介入术后合并重度心力衰竭的相关性及预后预测价值。方法选取2020年5月—2023年5月内蒙古包钢医院收治的80例急性心肌梗死冠脉介入术后合并心力衰竭患者作为研究对象,应用Killip分级来评价患者心力衰竭严重程度并进行分组,将其分为Ⅰ级组16例,Ⅱ级组23例,Ⅲ级组20例,Ⅳ级组21例,另选取同期来内蒙古包钢医院体检的30名健康志愿者作为对照组。对比五组患者PECAM-1与心室重构指标水平,应用Spearman相关分析PECAM-1、心室重构与急性心肌梗死冠脉介入术后合并重度心力衰竭的相关性。随后将80例急性心肌梗死冠脉介入术后合并心力衰竭患者中院内死亡者分为死亡组(n=20),将其余患者分为存活组(n=60),对比两组患者临床一般情况,并分析PECAM-1、心室重构对急性心肌梗死冠脉介入术后合并心力衰竭预后的预测价值。结果Ⅳ级患者PECAM-1、左心室质量指数(LVMI)明显高于Ⅲ级、Ⅱ级、Ⅰ级和对照组,左心室重构指数(LVRI)低于Ⅲ级、Ⅱ级、Ⅰ级和对照组(P<0.05);Spearman相关分析结果显示:PECAM-1、LVMI与急性心肌梗死冠脉介入术后合并重度心力衰竭呈正相关,LVRI与急性心肌梗死冠脉介入术后合并重度心力衰竭呈负相关(P<0.05);存活组与死亡组患者Killip分级、合并陈旧性心肌梗死、PECAM-1、LVRI、LVMI水平对比,差异有统计学意义(P<0.05);logistic回归分析结果表明:PECAM-1(OR=2.458,95%CI:1.359~3.257)、LVRI(OR=2.546,95%CI:1.364~3.475)、LVMI(OR=2.774,95%CI:1.876~4.010)为急性心肌梗死介入术后合并心力衰竭的独立预测指标(P<0.05)。结论PECAM-1、心室重构与急性心肌梗死冠脉介入术后合并重度心力衰竭具有明显相关性,且能够通过PECAM-1与心室重构情况来预测急性心肌梗死冠脉介入术后合并重度心力衰竭的预后情况。 展开更多
关键词 血小板内皮细胞黏附分子-1 心室重构 急性心肌梗死 冠脉介入 心力衰竭
下载PDF
腔隙性脑梗死患者血清PECAM-1、GDF11、FGF21与脑动脉血流动力学和颈动脉粥样硬化的关系 被引量:6
15
作者 王晓辉 陈红男 +3 位作者 蒋锋 常莎 王乐 崔小丽 《疑难病杂志》 CAS 2023年第3期258-265,共8页
目的 分析腔隙性脑梗死患者血清血小板内皮细胞黏附分子-1(PECAM-1)、生长分化因子11(GDF11)、成纤维细胞生长因子21(FGF21)与脑动脉血流动力学和颈动脉粥样硬化的关系。方法 选取2020年1月—2022年1月陕西省人民医院神经内科收治腔隙... 目的 分析腔隙性脑梗死患者血清血小板内皮细胞黏附分子-1(PECAM-1)、生长分化因子11(GDF11)、成纤维细胞生长因子21(FGF21)与脑动脉血流动力学和颈动脉粥样硬化的关系。方法 选取2020年1月—2022年1月陕西省人民医院神经内科收治腔隙性脑梗死患者160例为脑梗死组,并根据颈动脉内膜中层厚度(IMT)分为颈动脉粥样硬化亚组、颈动脉正常亚组,另选取同期健康体检者100例为健康对照组,比较2组血清PECAM-1、GDF11、FGF21水平和脑动脉血流动力学参数,采用Pearson线性相关性分析腔隙性脑梗死患者血清PECAM-1、GDF11、FGF21与脑动脉血流动力学参数的相关性。采用Logistic多因素回归分析腔隙性脑梗死患者发生颈动脉粥样硬化的影响因素,绘制受试者工作特征曲线(ROC)分析血清PECAM-1、GDF11、FGF21水平对颈动脉粥样硬化的诊断价值。结果 脑梗死组血清PECAM-1、FGF21高于健康对照组,血清GDF11低于健康对照组(t=8.386、17.511、6.795,P<0.001)。脑梗死组大脑中动脉、基底动脉搏动指数均高于健康对照组(t=16.928、7.686,P<0.001)。腔隙性脑梗死患者血清PECAM-1、FGF21水平与大脑中动脉、基底动脉搏动指数呈正相关(PECAM-1:r=0.537、0.462,P<0.001;FGF21:r=0.569、0.503,P<0.001),血清GDF11与大脑中动脉、基底动脉搏动指数呈负相关(r=-0.512、-0.498,P<0.001)。Logistic多因素回归分析结果显示,高血压史、低密度脂蛋白胆固醇(LDL-C)高、PECAM-1高、FGF21高是腔隙性脑梗死患者颈动脉粥样硬化发生的危险因素[OR(95%CI)=3.654(1.601~8.343)、2.481(1.508~4.085)、1.073(1.032~1.114)、1.024(1.011~1.038),P<0.01],血清高密度脂蛋白胆固醇(HDL-C)高、GDF11高是其保护因素[OR(95%CI)=0.781(0.613~0.995)、0.992(0.987~0.997),P<0.05];ROC曲线显示,血清PECAM-1、GDF11、FGF21及三者联合评估颈动脉粥样硬化发生风险的曲线下面积(AUC)分别为0.798、0.716、0.813、0.909,三者联合评估效能高于单项指标预测(Z/P=2.097/0.036,2.290/0.022,2.005/0.045)。结论 腔隙性脑梗死患者血清PECAM-1、GDF11、FGF21水平与脑动脉血流动力学参数有关,对颈动脉粥样硬化的评估具有重要意义。 展开更多
关键词 腔隙性脑梗死 血小板内皮细胞黏附分子-1 生长分化因子11 成纤维细胞生长因子21 脑动脉血流动力学 颈动脉粥样硬化 相关性
下载PDF
Adhesion molecule and proinflammatory cytokine gene expression in hepatic sinusoidal endothelial cells following cecal ligation and puncture 被引量:10
16
作者 Rong Qian Wu Ying Xin Xu +2 位作者 Xu Hua Song Li Jun Chen Xian Jun Meng Institute of Surgical Research, General Hospital of PLA, Beijing 100853, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期128-130,共3页
INTRODUCTIONMultiple organ dysfunction syndrome (MODS) isthought to be a frequent consequence of sepsis[1-3].Despite substantial advances in our knowledge and understanding of the basic pathophysiologic mechanisms[4-7... INTRODUCTIONMultiple organ dysfunction syndrome (MODS) isthought to be a frequent consequence of sepsis[1-3].Despite substantial advances in our knowledge and understanding of the basic pathophysiologic mechanisms[4-7], in critically ill patients infections and sepsis are still associated with a high mortality[8,9]. 展开更多
关键词 Animals CECUM Cytokines endotheliUM Gene Expression Intercellular adhesion molecule-1 INTERLEUKIN-1 Interleukin-6 LIGATION Liver Mice PUNCTURES RNA Messenger Research Support Non-U.S. Gov't Sepsis Tumor Necrosis Factor-alpha Vascular cell adhesion molecule-1
下载PDF
Pingyangmycin-regulated Expressions of Adhesion Molecules in Human Venous Malformation Endothelial Cells 被引量:2
17
作者 贾玉林 贾俊 赵怡芳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2012年第5期760-766,共7页
Pingyangmycin (bleomycin A5 hydrochloride,PYM) is one of the anti-neoplastic agents which have been commonly used to treat venous malformations.However,the underlying mechanism by which PYM treats venous malformations... Pingyangmycin (bleomycin A5 hydrochloride,PYM) is one of the anti-neoplastic agents which have been commonly used to treat venous malformations.However,the underlying mechanism by which PYM treats venous malformations remains poorly understood.It was reported that venous endothelial cells could recruit neutrophils via adhesion molecules (E-selectin,ICAM-1,ICAM-3,VCAM-1) during the acute/chronic inflammation and subsequent histological fibrosis after sclerotherapy with PYM.This study explored if the expression of E-selectin,ICAM-1,ICAM-3 and VCAM-1 in human venous malformation endothelial cells could be affected by PYM.HVMECs were cultured from human venous malformation tissue.Expressions of E-selectin,ICAM-1,ICAM-3 and VCAM-1 on HVMECs in response to PYM were analyzed by cell ELISA.The relative levels of mRNA expression in the cells were semi-quantified.The results showed that PYM up-regulated the expressions of E-selectin,ICAM-3,VCAM-1 and ICAM-1 in both time-and concentration-dependent manner.Our findings suggested that PYM could induce the expression of adhesion molecules in HVMECs,which might be a possible mechanism by which sclerotherapy by intralesional injection of PYM treats venous malformations. 展开更多
关键词 E-SELECTIN intercellular adhesion molecule-1 intercellular adhesion molecule-3 vascular cell adhesion molecule-1 cell culture
下载PDF
Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture 被引量:1
18
作者 Li-jie FAN Takeshi KARINO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第8期623-629,共7页
Background:The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially imp... Background:The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans. However, it is not well known how serum factors affect the adhesion of monocytes. Methods: We have studied the effect of fetal calf serum (FCS), which we considered a source of LDL, on the adhesion of monocytes to endothelial cells (ECs) by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells (EC monoculture) and a co-culture with bovine aortic smooth muscle cells (EC-SMC co-culture). Results: It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells, and the higher the concentration of FCS in the medium, the greater the adhesion of THP-1 cells to endothelial cells. Adhesion of THP-1 cells to an EC-SMC co-culture was approximately twofold greater than that to an EC monoculture, and after adhering to endothelial cells, many THP-1 cells trans-migrated into the layer of smooth muscle cells. Conclusion: The results suggest that the elevation of the LDL (cholesterol) level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytes to the endothelium and their subsequent migration into subendothelial spaces. 展开更多
关键词 endothelial cells (ECs) Smooth muscle cells (SMCs) MONOCYTE THP-1 cells Low-density lipoprotein (LDL) adhesion Transendothelial migration Serum concentration
下载PDF
Effects of Sirt1 on proliferation,migration,and apoptosis of endothelial progenitor cells in peripheral blood of SD rats with chronic obstructive pulmonary disease 被引量:1
19
作者 Dong-Mei Sun Jin-Jian Yao +4 位作者 Zhan-Ling Dong Jin Qian Qi-Feng Huang Yuan-Tian Sun Xiao-Ran Liu 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第10期429-439,共11页
Objective:To explore the effect of Sirt1 on the function of endothelial progenitor cells(EPCs)in rats with chronic obstructive pulmonary disease(COPD).Methods:A rat COPD model was established via smoking and endotoxin... Objective:To explore the effect of Sirt1 on the function of endothelial progenitor cells(EPCs)in rats with chronic obstructive pulmonary disease(COPD).Methods:A rat COPD model was established via smoking and endotoxin administration for three months.The peripheral circulating EPCs were isolated by gradient centrifugation,and their functions,cell cycle distribution,apoptosis,and Sirt1 expression were examined.The function changes of EPCs in the presence or absence of Sirt1 agonist and inhibitor were estimated;meanwhile,the expressions of Sirt1,FOXO3a,NF-κB,and p53 were also evaluated.Results:The proliferation,adhesion,and migration of EPCs decreased while the apoptosis rate was increased in the COPD rats.The expression of Sirt1 protein in EPCs of the COPD group was significantly lower than that in the control group(P<0.01).The overexpression of the Sirt1 gene using a gene transfection technique or Sirt1 agonists(SRT1720)improved the proliferation,migration,and adhesion,and decreased the apoptosis of EPC.However,Sirt1 inhibitor(EX527)decreased EPC functions in the COPD group.The effect of Sirt1 expression on EPC function may be related to reduction of FOXO3a and increase of NF-κB and p53 activity.Conclusions:Increased expression of Sirt1 can improve the proliferation and migration of EPCs and reduce their apoptosis in COPD rats.This change may be related to FOXO3a,NF-κB,and p53 signaling pathways. 展开更多
关键词 Chronic obstructive pulmonary disease endothelial progenitor cells SIRT1 PROLIFERATION MIGRATION adhesion APOPTOSIS FOXO3a NF-κB p53 Rat
下载PDF
血小板内皮细胞黏附分子1和平滑肌肌动蛋白对瘢痕疙瘩综合治疗预后的影响 被引量:1
20
作者 羊逸飞 许辉 +1 位作者 龙卫国 李遇梅 《江苏大学学报(医学版)》 CAS 2024年第1期57-60,共4页
目的:探究瘢痕疙瘩血管内血小板内皮细胞黏附分子1(platelet endothelial cell adhesion molecule-1,PECAM-1)与平滑肌肌动蛋白(smooth muscle actin,SMA)的表达水平对瘢痕疙瘩综合治疗预后的影响。方法:回顾性分析2020年8月至2022年6... 目的:探究瘢痕疙瘩血管内血小板内皮细胞黏附分子1(platelet endothelial cell adhesion molecule-1,PECAM-1)与平滑肌肌动蛋白(smooth muscle actin,SMA)的表达水平对瘢痕疙瘩综合治疗预后的影响。方法:回顾性分析2020年8月至2022年6月江苏大学附属医院皮肤科门诊收治的瘢痕疙瘩患者61例,共计69处瘢痕疙瘩,均接受手术切除与^(90)Sr同位素敷贴综合治疗,免疫组织化学染色检测手术切除瘢痕疙瘩标本血管内PECAM-1及SMA表达水平,电话及门诊随访6个月。结果:19处(27.5%)瘢痕疙瘩6个月内复发,11处(15.9%)在^(90)Sr同位素敷贴治疗后发生不良反应,复发组PECAM-1与SMA的高表达率均高于未复发组(χ^(2)=7.496,P=0.006;χ^(2)=5.197,P=0.023);治疗后不良反应发生率与PECAM-1及SMA的表达水平无明显关系(χ^(2)=0.172,P=0.935;χ^(2)=1.110,P=0.484)。结论:瘢痕疙瘩血管内PECAM-1及SMA的表达水平与综合治疗预后呈负相关,二者可能是判断瘢痕疙瘩预后的潜在指标。 展开更多
关键词 瘢痕疙瘩 放射性核素敷贴治疗 免疫组织化学染色 血小板内皮细胞黏附分子1 平滑肌肌动蛋白
下载PDF
上一页 1 2 25 下一页 到第
使用帮助 返回顶部