An experiment was conducted to investigate the requirement of nonphytate phosphorus(nPP) and efficacy of a genetically engineered yeast phytase(PHY A) for Lingnan yellow broilers from 22-to 42-d-old age.A total of...An experiment was conducted to investigate the requirement of nonphytate phosphorus(nPP) and efficacy of a genetically engineered yeast phytase(PHY A) for Lingnan yellow broilers from 22-to 42-d-old age.A total of 1 320 1-d-old male chicks were randomly divided into 11 dietary treatment groups,which consisted of 4 replicate floor pens with 30 birds per pen.The control group(treatment 1) was fed with basal diet of nPP 0.08% without dicalcium phosphate or phytase supplementation.Dietary levels of nPP were 0.16,0.24,0.32,0.40,0.48,and 0.56%,respectively,for treatments 2 to 7,through addition of dicalcium phosphate(chemistry grade) to the basal diet.Diets of treatments 8 to 11 were supplemented with PHY A at 200,400 and 600 U kg-1,a commercial phytase product(PHY B) at 400 U kg-1 level,respectively.The birds in 0.32-0.56% nPP groups gained more than those of the other groups(P0.05).The nPP supplementation significantly improved feed intake(P0.05).The feed gain ratio was significantly decreased by 0.40% nPP diet compared to the control birds(P0.05).The level of 0.48% nPP was required for optimum tibia development.The additions of PHY A at 400 and 600 U kg-1 level and PHY B all significantly improved ADG(P0.05),ADFI(P0.05),and dry defatted tibia weight(P0.05).Similarly,the percentage of tibia ash was increased by 600 U kg-1 PHY A supplementation(P0.05).The requirement of nPP for maximal ADG and highest percentage tibia ash both was 0.40%.The phosphorus equivalency value of PHY A was estimated as 685 U kg-1 for male yellow broilers of 22-to 42-d-old age.展开更多
Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, met...Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory forproduction of proteins and high-value chemicals. Methanol utilization pathway (MUT) is highlyregulated for efficient methanol utilization, and the downstream pathways need extensively constructedand optimized toward target metabolite biosynthesis. Here, we present an overview of methanolmetabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of keygenes involved in methanol metabolism. Besides, the recent progresses in construction and optimizationof downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fattyacids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasiblestrategies toward constructing efficient methylotrophic cell factories may promote wide applications inthe future.展开更多
基金supported by the the Earmarked Fund for Modern Agro-Industry Technology Research System,China (nycytx-42-G2-01)the grant from Ministry of Agriculture of China (nyhyzx07-038)
文摘An experiment was conducted to investigate the requirement of nonphytate phosphorus(nPP) and efficacy of a genetically engineered yeast phytase(PHY A) for Lingnan yellow broilers from 22-to 42-d-old age.A total of 1 320 1-d-old male chicks were randomly divided into 11 dietary treatment groups,which consisted of 4 replicate floor pens with 30 birds per pen.The control group(treatment 1) was fed with basal diet of nPP 0.08% without dicalcium phosphate or phytase supplementation.Dietary levels of nPP were 0.16,0.24,0.32,0.40,0.48,and 0.56%,respectively,for treatments 2 to 7,through addition of dicalcium phosphate(chemistry grade) to the basal diet.Diets of treatments 8 to 11 were supplemented with PHY A at 200,400 and 600 U kg-1,a commercial phytase product(PHY B) at 400 U kg-1 level,respectively.The birds in 0.32-0.56% nPP groups gained more than those of the other groups(P0.05).The nPP supplementation significantly improved feed intake(P0.05).The feed gain ratio was significantly decreased by 0.40% nPP diet compared to the control birds(P0.05).The level of 0.48% nPP was required for optimum tibia development.The additions of PHY A at 400 and 600 U kg-1 level and PHY B all significantly improved ADG(P0.05),ADFI(P0.05),and dry defatted tibia weight(P0.05).Similarly,the percentage of tibia ash was increased by 600 U kg-1 PHY A supplementation(P0.05).The requirement of nPP for maximal ADG and highest percentage tibia ash both was 0.40%.The phosphorus equivalency value of PHY A was estimated as 685 U kg-1 for male yellow broilers of 22-to 42-d-old age.
基金funded by the Young Investigator Grant from Dalian Institute of Chemicals Physics,Chinese Academy of Sciences(to Y.J.Zhou)
文摘Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory forproduction of proteins and high-value chemicals. Methanol utilization pathway (MUT) is highlyregulated for efficient methanol utilization, and the downstream pathways need extensively constructedand optimized toward target metabolite biosynthesis. Here, we present an overview of methanolmetabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of keygenes involved in methanol metabolism. Besides, the recent progresses in construction and optimizationof downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fattyacids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasiblestrategies toward constructing efficient methylotrophic cell factories may promote wide applications inthe future.