Enhancement effects of rare earth elements on the growth of Chaetoceros mulleri is studied in this paper. The results show that all of the light, middle and heavy rare earth elements have similar enhancement effect on...Enhancement effects of rare earth elements on the growth of Chaetoceros mulleri is studied in this paper. The results show that all of the light, middle and heavy rare earth elements have similar enhancement effect on the growth of Chaetoceros mulleri, with the beneficial concentrations of La, Gd and Yb being 7.28~87.40 μ mol/L, 6.36~57.23 μ mol/L and 5.78~17.34 μ mol/L. The optimum concentrations of La, Gd and Yb are 7.28 ~50.98 μ mol/L,31.80~44.52 μ m ol/L and 5.78~17.34 1μ mol/L, respectively. When the concentrations of La, Gd and Yb are 7.28~87.40 μ mol/L, 6.36~57.23 μ mol/L and 5.78~ 17,34 μ mol/L, the concentrations of chlorophyll have increased by 9.3~47.0%, 33.4~44.3%, and 36.5~40.3%, respectively as compared with the control group. The mechanism of enhancement of rare earth elements on the growth ot Chaetoceros mulleri is also discussed in this paper.展开更多
Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of...Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter gene (d2EGFP) has been used to analyse the role of certain defined putative elements and a downstream enhancer element in gene expression. These experiments were carried out in vitro using a line of pre-pubertal mouse Sertoli cells, transienly transfected with circular DNA constructs with variously mutated promoter elements. A downstream enhancer element, situated immediately 3’ of the polyadenylation (PA) signal for Amh, has been inserted in an equivalent position in the d2EGFP construct. When the Amh promoter is unmodified, the downstream enhancer (DE) is positively associated with a large increase in EGFP expression. This is at least partly the consequence of an increased rate of expression by individual cells. Experiments using variously truncated Amh promoters indicate that an upstream region (-214 to -336) may play a minor role in facilitating enhancement. However mutation of the Wilms tumour factor-1 element, situated between the tata box and the start of translation, results in an almost complete suppression of enhancement.展开更多
An one-year of sampling aerosol program was carried out during the period of April 1995 to April 1996 at coastal region, Cape D′Aguilar, in Hong Kong. The trace element composition of aerosol particles(TSP and PM ...An one-year of sampling aerosol program was carried out during the period of April 1995 to April 1996 at coastal region, Cape D′Aguilar, in Hong Kong. The trace element composition of aerosol particles(TSP and PM 10 ) were measured and analyzed by using the Instrumental Neutron Activation Analysis(INAA) and Inductive Couple Plasma-Mass Spectrometry(ICP-MS) techniques. The results showed that the polluted elements such as: V, Zn, As, Pb, Cd, Sb, I, Se, Bi, with high enrichment factor(E.F) values, are derived from anthropogenic sources, while Sc, Al, Fe, Mn, Sm, La, Sr are less than 10 in E.F, these elements are related the crust and soil. The concentration of the trace elements are present seasonal variation i.e. summer low and winter high, whereas the V or noncrustal V(V *) present summer high and winter low, this phenomenon might be explained by local or regional oil combustion sources. Use three type of elemental ratio, e.g. La/Sm, Mn */V * and Bi/Al indicate that both TSP and PM 10 of La/Sm ratios are mainly related to soil and dust, Mn */V * are probably represent fossil combustion and Bi/Al are possibly associated with refuse incineration, ferromanganese alloys and aluminum production. The varimax rotation factor analysis for trace elements was performed. By means of the absolute principal component analysis(APCA) and multiple regression, the contribution of trace elements to possible sources and overall means of trace elements were estimated. The results showed that the present station is impacted by the anthropogenic species, although the quantities are different during the summertime and wintertime.展开更多
A bandwidth-enhanced dual-polarized antenna is proposed for 2/3/4/5G applications,which is composed of distributed parasitic elements(DPEs),a main radiator,two improved broadband integrated baluns and a reflector.Firs...A bandwidth-enhanced dual-polarized antenna is proposed for 2/3/4/5G applications,which is composed of distributed parasitic elements(DPEs),a main radiator,two improved broadband integrated baluns and a reflector.First,a novel tooth-shape shorted slot line in the improved broadband integrated balun is analyzed to adjust the input impedance of the antenna.Then,DPEs with 2×2 circular plates loading over the main radiator are proposed to improve broadband impedance matching and radiation pattern.By utilizing impedance compensation of the tooth-shaped shorted slot line and the electromagnetic induction of the DPEs,the antenna achieves an enhanced impedance bandwidth and a stable radiation pattern.To verify these ideas,the bandwidth-enhanced dual-polarized antenna was fabricated and measured.The experimental results indicate that the proposed antenna achieves an operating bandwidth of 72.2%(1.69 to 3.60 GHz)with a return loss(RL)less than-15 dB and a port-to-port isolation(ISO)larger than 30 dB.The antenna obtains a half-power beamwidth(HPBW)within(66±5)°and a gain within(9.0±0.6)dBi in the 2/3/4G bands,and an HPBW within(61.5±2.5)°and a gain within(9.8±0.3)dBi in the 5G band.Across the whole band,the cross-polarization discrimination(XPD)and the front-to-back ratio are both larger than 20 dB.展开更多
This study examines the heat transfer enhancement from a horizontal rectangular fin embedded with triangular perforations (their bases parallel and toward the fin tip) under natural convection. The fin's heat dissi...This study examines the heat transfer enhancement from a horizontal rectangular fin embedded with triangular perforations (their bases parallel and toward the fin tip) under natural convection. The fin's heat dissipation rate is compared to that of an equivalent solid one. The parameters considered are geometrical dimensions and thermal properties of the fin and the perforations. The gain in the heat transfer enhancement and the fin weight reduction due to the perforations are considered. The study shows that the heat dissipation from the perforated fin for a certain range of triangular perforation dimensions and spaces between perforations result in improvement in the heat transfer over the equivalent solid fin. The heat transfer enhancement of the perforated fin increases as the fin thermal conductivity and its thickness are increased.展开更多
Fluorescence and cofluorescence properties of Tb(Ⅲ) solid complexes werestudied using pyromellitic acid (PMA) as ligand and fluorescence inert ions as doping elements. Thecofluorescence enhancement, a result of ligan...Fluorescence and cofluorescence properties of Tb(Ⅲ) solid complexes werestudied using pyromellitic acid (PMA) as ligand and fluorescence inert ions as doping elements. Thecofluorescence enhancement, a result of ligand sensitized fluorescence, was observed in Tb(Ⅲ) solidcomplexes doped with fluorescent inert ions La(Ⅲ), Gd(Ⅲ), Ca(Ⅲ), and Sr(Ⅲ). The effect of thetype and content of doping elements on fluorescence enhancement was studied, and optimum conditionswere determined. The results show that Gd (La, Ca, Sr) has clear cofluorescence effect in solidcomplex Tb-M-PMA system, and in present work, rare earth complex fluorescent powder that emitsbright green fluorescence at ultraviolet excitation was obtained, which had potential application asfluorescent anti-counterfeit ink.展开更多
The special formulation that allows an accurate and efficient solution to the heat transfer problems within fin assemblies with very large aspect ratios has been developed in this paper Numerically,it consists of the ...The special formulation that allows an accurate and efficient solution to the heat transfer problems within fin assemblies with very large aspect ratios has been developed in this paper Numerically,it consists of the boundary element method in the wall region and the analytical solution in the fin region This modified BEM makes tractable a large class of heat transfer problems in the long and thin domains which are frequently encountered in practice.展开更多
The basic idea of quasi-conforming method is that the strain-dis-placement equations are weakened as well as the equilibrium equations.In this paper,an 18-DOF triangular element for couple stress theory is proposed wi...The basic idea of quasi-conforming method is that the strain-dis-placement equations are weakened as well as the equilibrium equations.In this paper,an 18-DOF triangular element for couple stress theory is proposed within the framework of quasi-conforming technique.The formulation starts from truncated Taylor expansion of strains and appropriate interpolation functions are chosen to calculate strain integration.This element satisfies C0 continuity with second order accuracy and weak C1 continuity simultaneously.Numerical examples demonstrate that the proposed model can pass the C0??1 patch test and has high accuracy.The element does not exhibit extra zero energy modes and can capture the scale effects of microstructure.展开更多
Objectives: Enhanced infrared neural stimulation (EINS) using nanoparticles is a new research hotspot. In this paper, the numerical modeling of the interaction between a light source and brain tissue during EINS is st...Objectives: Enhanced infrared neural stimulation (EINS) using nanoparticles is a new research hotspot. In this paper, the numerical modeling of the interaction between a light source and brain tissue during EINS is studied. Materials and Methods: This model is built with the finite element method (FEM) to mimic the propagation and absorption of light in brain tissue with EINS. Only the thermal change is considered in this model since the photothermal effect is the main mechanism of EINS. The temperature response of brain irradiation is governed by the extensively used Pennes’ bio-heat equation in a multilayer model. Results: The temperature distribution in the brain under laser irradiation is determined. And the relationships between the brain tissue temperature and the three factors (the laser pulse time, the laser energy and the enhanced absorption coefficient of the tissue caused by the nanoparticles) are analyzed. Conclusions: The results indicate that the brain tissue is easier to warm up with the enhancement of nanoparticles and parameters of the laser can alter the temperature increase of the brain tissue. These findings offer a theoretical basis for future animal experiments.展开更多
Buckling and postbuckling characteristics of laminated graphene-enhanced composite(GEC)truncated conical shells exposed to torsion under temperature conditions using finite element method(FEM)simulation are presented ...Buckling and postbuckling characteristics of laminated graphene-enhanced composite(GEC)truncated conical shells exposed to torsion under temperature conditions using finite element method(FEM)simulation are presented in this study.In the thickness direction,the GEC layers of the conical shell are ordered in a piece-wise arrangement of functionally graded(FG)distribution,with each layer containing a variable volume fraction for graphene reinforcement.To calculate the properties of temperaturedependent material of GEC layers,the extended Halpin-Tsai micromechanical framework is used.The FEM model is verified via comparing the current results obtained with the theoretical estimates for homogeneous,laminated cylindrical,and conical shells,the FEM model is validated.The computational results show that a piece-wise FG graphene volume fraction distribution can improve the torque of critical buckling and torsional postbuckling strength.Also,the geometric parameters have a critical impact on the stability of the conical shell.However,a temperature rise can reduce the crucial torsional buckling torque as well as the GEC laminated truncated conical shell’s postbuckling strength.展开更多
Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) ...Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) located in the env gene through their cis-acting targets. We have identified and characterized a 72 bp TBS (Borf-1) responsive element located in BFV3026, internal promoter (TREIP) by deletion mutant and transient expression assay. This cis-acting target element in the internal promoter has the properties of a transcriptional enhancer which functions independently of its orientation, position and also in heterologous promoters (BFV LTR and bovine immunodeficiency virus, BIV LTR). Alignments reveal that there are positional similarity and sequence homology among BFV TREIP, SFV-1 TREIP proximal element and SFV-3 TREIPH, which suggests that this kind of cis-acting elements possesses some common functional character.展开更多
Enhancers are DNA sequences that can strengthen transcription initiation.However,the global identification of plant enhancers is complicated due to uncertainty in the distance and orientation of enhancers,especially i...Enhancers are DNA sequences that can strengthen transcription initiation.However,the global identification of plant enhancers is complicated due to uncertainty in the distance and orientation of enhancers,especially in species with large genomes.In this study,we performed self-transcribing active regulatory region sequencing(STARR-seq)for the first time to identify enhancers across the barley genome.A total of 7323 enhancers were successfully identified,and among 45 randomly selected enhancers,over 75%were effective as validated by a dual-luciferase reporter assay system in the lower epidermis of tobacco leaves.Interestingly,up to 53.5%of the barley enhancers were repetitive sequences,especially transposable elements(TEs),thus reinforcing the vital role of repetitive enhancers in gene expression.Both the common active mark H3K4me3 and repressive mark H3K27me3 were abundant among the barley STARR-seq enhancers.In addition,the functional range of barley STARR-seq enhancers seemed much broader than that of rice or maize and extended to±100 kb of the gene body,and this finding was consistent with the high expression levels of genes in the genome.This study specifically depicts the unique features of barley enhancers and provides available barley enhancers for further utilization.展开更多
基金Supported by National Natural Science Fund of China (No: 39370548)
文摘Enhancement effects of rare earth elements on the growth of Chaetoceros mulleri is studied in this paper. The results show that all of the light, middle and heavy rare earth elements have similar enhancement effect on the growth of Chaetoceros mulleri, with the beneficial concentrations of La, Gd and Yb being 7.28~87.40 μ mol/L, 6.36~57.23 μ mol/L and 5.78~17.34 μ mol/L. The optimum concentrations of La, Gd and Yb are 7.28 ~50.98 μ mol/L,31.80~44.52 μ m ol/L and 5.78~17.34 1μ mol/L, respectively. When the concentrations of La, Gd and Yb are 7.28~87.40 μ mol/L, 6.36~57.23 μ mol/L and 5.78~ 17,34 μ mol/L, the concentrations of chlorophyll have increased by 9.3~47.0%, 33.4~44.3%, and 36.5~40.3%, respectively as compared with the control group. The mechanism of enhancement of rare earth elements on the growth ot Chaetoceros mulleri is also discussed in this paper.
文摘Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter gene (d2EGFP) has been used to analyse the role of certain defined putative elements and a downstream enhancer element in gene expression. These experiments were carried out in vitro using a line of pre-pubertal mouse Sertoli cells, transienly transfected with circular DNA constructs with variously mutated promoter elements. A downstream enhancer element, situated immediately 3’ of the polyadenylation (PA) signal for Amh, has been inserted in an equivalent position in the d2EGFP construct. When the Amh promoter is unmodified, the downstream enhancer (DE) is positively associated with a large increase in EGFP expression. This is at least partly the consequence of an increased rate of expression by individual cells. Experiments using variously truncated Amh promoters indicate that an upstream region (-214 to -336) may play a minor role in facilitating enhancement. However mutation of the Wilms tumour factor-1 element, situated between the tata box and the start of translation, results in an almost complete suppression of enhancement.
文摘An one-year of sampling aerosol program was carried out during the period of April 1995 to April 1996 at coastal region, Cape D′Aguilar, in Hong Kong. The trace element composition of aerosol particles(TSP and PM 10 ) were measured and analyzed by using the Instrumental Neutron Activation Analysis(INAA) and Inductive Couple Plasma-Mass Spectrometry(ICP-MS) techniques. The results showed that the polluted elements such as: V, Zn, As, Pb, Cd, Sb, I, Se, Bi, with high enrichment factor(E.F) values, are derived from anthropogenic sources, while Sc, Al, Fe, Mn, Sm, La, Sr are less than 10 in E.F, these elements are related the crust and soil. The concentration of the trace elements are present seasonal variation i.e. summer low and winter high, whereas the V or noncrustal V(V *) present summer high and winter low, this phenomenon might be explained by local or regional oil combustion sources. Use three type of elemental ratio, e.g. La/Sm, Mn */V * and Bi/Al indicate that both TSP and PM 10 of La/Sm ratios are mainly related to soil and dust, Mn */V * are probably represent fossil combustion and Bi/Al are possibly associated with refuse incineration, ferromanganese alloys and aluminum production. The varimax rotation factor analysis for trace elements was performed. By means of the absolute principal component analysis(APCA) and multiple regression, the contribution of trace elements to possible sources and overall means of trace elements were estimated. The results showed that the present station is impacted by the anthropogenic species, although the quantities are different during the summertime and wintertime.
基金The National Natural Science Foundation of China(No.61471117).
文摘A bandwidth-enhanced dual-polarized antenna is proposed for 2/3/4/5G applications,which is composed of distributed parasitic elements(DPEs),a main radiator,two improved broadband integrated baluns and a reflector.First,a novel tooth-shape shorted slot line in the improved broadband integrated balun is analyzed to adjust the input impedance of the antenna.Then,DPEs with 2×2 circular plates loading over the main radiator are proposed to improve broadband impedance matching and radiation pattern.By utilizing impedance compensation of the tooth-shaped shorted slot line and the electromagnetic induction of the DPEs,the antenna achieves an enhanced impedance bandwidth and a stable radiation pattern.To verify these ideas,the bandwidth-enhanced dual-polarized antenna was fabricated and measured.The experimental results indicate that the proposed antenna achieves an operating bandwidth of 72.2%(1.69 to 3.60 GHz)with a return loss(RL)less than-15 dB and a port-to-port isolation(ISO)larger than 30 dB.The antenna obtains a half-power beamwidth(HPBW)within(66±5)°and a gain within(9.0±0.6)dBi in the 2/3/4G bands,and an HPBW within(61.5±2.5)°and a gain within(9.8±0.3)dBi in the 5G band.Across the whole band,the cross-polarization discrimination(XPD)and the front-to-back ratio are both larger than 20 dB.
文摘This study examines the heat transfer enhancement from a horizontal rectangular fin embedded with triangular perforations (their bases parallel and toward the fin tip) under natural convection. The fin's heat dissipation rate is compared to that of an equivalent solid one. The parameters considered are geometrical dimensions and thermal properties of the fin and the perforations. The gain in the heat transfer enhancement and the fin weight reduction due to the perforations are considered. The study shows that the heat dissipation from the perforated fin for a certain range of triangular perforation dimensions and spaces between perforations result in improvement in the heat transfer over the equivalent solid fin. The heat transfer enhancement of the perforated fin increases as the fin thermal conductivity and its thickness are increased.
基金This work is financially supported by the Natural Science Foundation of Hunan Province (No. 01JJY3004) and Technology of China National Packaging Corporation.
文摘Fluorescence and cofluorescence properties of Tb(Ⅲ) solid complexes werestudied using pyromellitic acid (PMA) as ligand and fluorescence inert ions as doping elements. Thecofluorescence enhancement, a result of ligand sensitized fluorescence, was observed in Tb(Ⅲ) solidcomplexes doped with fluorescent inert ions La(Ⅲ), Gd(Ⅲ), Ca(Ⅲ), and Sr(Ⅲ). The effect of thetype and content of doping elements on fluorescence enhancement was studied, and optimum conditionswere determined. The results show that Gd (La, Ca, Sr) has clear cofluorescence effect in solidcomplex Tb-M-PMA system, and in present work, rare earth complex fluorescent powder that emitsbright green fluorescence at ultraviolet excitation was obtained, which had potential application asfluorescent anti-counterfeit ink.
文摘The special formulation that allows an accurate and efficient solution to the heat transfer problems within fin assemblies with very large aspect ratios has been developed in this paper Numerically,it consists of the boundary element method in the wall region and the analytical solution in the fin region This modified BEM makes tractable a large class of heat transfer problems in the long and thin domains which are frequently encountered in practice.
基金the Fundamental Research Funds for the Central Universities(DUT14RC(3)092)the National Natural Science Foundation of China(No.11272075,11472071).
文摘The basic idea of quasi-conforming method is that the strain-dis-placement equations are weakened as well as the equilibrium equations.In this paper,an 18-DOF triangular element for couple stress theory is proposed within the framework of quasi-conforming technique.The formulation starts from truncated Taylor expansion of strains and appropriate interpolation functions are chosen to calculate strain integration.This element satisfies C0 continuity with second order accuracy and weak C1 continuity simultaneously.Numerical examples demonstrate that the proposed model can pass the C0??1 patch test and has high accuracy.The element does not exhibit extra zero energy modes and can capture the scale effects of microstructure.
文摘Objectives: Enhanced infrared neural stimulation (EINS) using nanoparticles is a new research hotspot. In this paper, the numerical modeling of the interaction between a light source and brain tissue during EINS is studied. Materials and Methods: This model is built with the finite element method (FEM) to mimic the propagation and absorption of light in brain tissue with EINS. Only the thermal change is considered in this model since the photothermal effect is the main mechanism of EINS. The temperature response of brain irradiation is governed by the extensively used Pennes’ bio-heat equation in a multilayer model. Results: The temperature distribution in the brain under laser irradiation is determined. And the relationships between the brain tissue temperature and the three factors (the laser pulse time, the laser energy and the enhanced absorption coefficient of the tissue caused by the nanoparticles) are analyzed. Conclusions: The results indicate that the brain tissue is easier to warm up with the enhancement of nanoparticles and parameters of the laser can alter the temperature increase of the brain tissue. These findings offer a theoretical basis for future animal experiments.
文摘Buckling and postbuckling characteristics of laminated graphene-enhanced composite(GEC)truncated conical shells exposed to torsion under temperature conditions using finite element method(FEM)simulation are presented in this study.In the thickness direction,the GEC layers of the conical shell are ordered in a piece-wise arrangement of functionally graded(FG)distribution,with each layer containing a variable volume fraction for graphene reinforcement.To calculate the properties of temperaturedependent material of GEC layers,the extended Halpin-Tsai micromechanical framework is used.The FEM model is verified via comparing the current results obtained with the theoretical estimates for homogeneous,laminated cylindrical,and conical shells,the FEM model is validated.The computational results show that a piece-wise FG graphene volume fraction distribution can improve the torque of critical buckling and torsional postbuckling strength.Also,the geometric parameters have a critical impact on the stability of the conical shell.However,a temperature rise can reduce the crucial torsional buckling torque as well as the GEC laminated truncated conical shell’s postbuckling strength.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39970033 ) Doctoral Fund in High School (Grant No. 98005516).
文摘Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) located in the env gene through their cis-acting targets. We have identified and characterized a 72 bp TBS (Borf-1) responsive element located in BFV3026, internal promoter (TREIP) by deletion mutant and transient expression assay. This cis-acting target element in the internal promoter has the properties of a transcriptional enhancer which functions independently of its orientation, position and also in heterologous promoters (BFV LTR and bovine immunodeficiency virus, BIV LTR). Alignments reveal that there are positional similarity and sequence homology among BFV TREIP, SFV-1 TREIP proximal element and SFV-3 TREIPH, which suggests that this kind of cis-acting elements possesses some common functional character.
基金supported by the grants from the Key Program of Sichuan Province Natural Science Foundation(Grant No.2022NSFSC0015)the Key R&D Program of Sichuan Province(Grant Nos.2021YFN0034 and 2021YFG0028),China.
文摘Enhancers are DNA sequences that can strengthen transcription initiation.However,the global identification of plant enhancers is complicated due to uncertainty in the distance and orientation of enhancers,especially in species with large genomes.In this study,we performed self-transcribing active regulatory region sequencing(STARR-seq)for the first time to identify enhancers across the barley genome.A total of 7323 enhancers were successfully identified,and among 45 randomly selected enhancers,over 75%were effective as validated by a dual-luciferase reporter assay system in the lower epidermis of tobacco leaves.Interestingly,up to 53.5%of the barley enhancers were repetitive sequences,especially transposable elements(TEs),thus reinforcing the vital role of repetitive enhancers in gene expression.Both the common active mark H3K4me3 and repressive mark H3K27me3 were abundant among the barley STARR-seq enhancers.In addition,the functional range of barley STARR-seq enhancers seemed much broader than that of rice or maize and extended to±100 kb of the gene body,and this finding was consistent with the high expression levels of genes in the genome.This study specifically depicts the unique features of barley enhancers and provides available barley enhancers for further utilization.