The enteric nervous system （ENS） controls the function of the gastrointestinal tract and has been impli- cated in various diseases, including Parkinson＇s disease （PD）. PD is a neurodegenerative disease with Lewy ...The enteric nervous system （ENS） controls the function of the gastrointestinal tract and has been impli- cated in various diseases, including Parkinson＇s disease （PD）. PD is a neurodegenerative disease with Lewy bodies （LBs） and Lewy neurites （LNs） as the main pathological features. In addition to the typical motor symptoms in PD, attention has been drawn to non-motor symptoms, such as constipation, implying dysfunction of the ENS. In the present study, we characterized the age-dependent mor- phological alterations and aggregation of α-synuclein （α- syn）, the primary protein component in LBs and LNs, in the ENS in an α-syn transgenic mouse model. We found that the expression and accumulation of α-syn increased gradually in neurons of Meissner＇s and Auerbach＇s plexuses of the gastrointestinal tract with age （from 1 week to 2 years）. In addition, α-syn was increasingly phosphorylated at the serine 129 residue, reflecting patho- logical alterations of the protein over time. Furthermore,α- syn was present in different subtypes of neurons expressing vasoactive intestinal polypeptide, neuronal nitric oxide synthase, or calretinin. The results indicated that BAC-α- Syn-GFP transgenic mice provide a unique model in which to study the relationship between ENS and PD pathogenesis.展开更多
During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mo...During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease,sometimes responsible for causing severe economic loss to fish farming industry.The disease is clinically characterized by symptoms like bleeding at the base of fins,inside and around mouth,bilateral exophthalmia with or without haemorrhage,and per-ocular and peri-oral haemorrhages.In the present study,live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques.Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction.DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y.ruckeri specific primers.The PCR product was run on 1.5%agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected.It is similar to that given for PCR products with primers specific for Y.ruckeri.展开更多
基金supported by the National Natural Science Foundation of China (81430025)Northeastern University, China, as well as the Swedish Research Council (K201561X-22297-03-4)+5 种基金the EU Joint Programme-Neurodegenerative Disease Research (aSyn Protec and REfre AME),EU H2020-MSCAITN-2016 (Syndegen)Basal Ganglia Disorders Linnaeus Consortium-Excellence in Parkinson and Huntington Researchthe Strong Research Environment Multi Park (Multidisciplinary Research on Parkinson's disease)the Swedish Parkinson Foundation (Parkinsonfonden)the Torsten Sderbergs Foundation, and the Olle Engkvist Byggmstere Foundationsupported by a scholarship from the China Scholarship Council
文摘The enteric nervous system （ENS） controls the function of the gastrointestinal tract and has been impli- cated in various diseases, including Parkinson＇s disease （PD）. PD is a neurodegenerative disease with Lewy bodies （LBs） and Lewy neurites （LNs） as the main pathological features. In addition to the typical motor symptoms in PD, attention has been drawn to non-motor symptoms, such as constipation, implying dysfunction of the ENS. In the present study, we characterized the age-dependent mor- phological alterations and aggregation of α-synuclein （α- syn）, the primary protein component in LBs and LNs, in the ENS in an α-syn transgenic mouse model. We found that the expression and accumulation of α-syn increased gradually in neurons of Meissner＇s and Auerbach＇s plexuses of the gastrointestinal tract with age （from 1 week to 2 years）. In addition, α-syn was increasingly phosphorylated at the serine 129 residue, reflecting patho- logical alterations of the protein over time. Furthermore,α- syn was present in different subtypes of neurons expressing vasoactive intestinal polypeptide, neuronal nitric oxide synthase, or calretinin. The results indicated that BAC-α- Syn-GFP transgenic mice provide a unique model in which to study the relationship between ENS and PD pathogenesis.
基金The financial support was provided by a fellowship Grant from“Maulana Azad National Fellowship Programme”New Delhi,India.
文摘During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease,sometimes responsible for causing severe economic loss to fish farming industry.The disease is clinically characterized by symptoms like bleeding at the base of fins,inside and around mouth,bilateral exophthalmia with or without haemorrhage,and per-ocular and peri-oral haemorrhages.In the present study,live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques.Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction.DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y.ruckeri specific primers.The PCR product was run on 1.5%agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected.It is similar to that given for PCR products with primers specific for Y.ruckeri.
