The environmental signal of an early Holocene pollen record from the Shiyang River basin lake sediments, NW China

Pollen analyses of 85 samples from the San-jiaocheng section well along the margin of a palaeolake at the end of the Shiyang River, NW China, show that Picea and Sabina dominate the pollen assemblage. Together they reach as high as 40%-60%, with the percentage of Picea varying inversely with that of Sabina. Similar results were obtained from another section in the Shiyang River drainage. Using modern ecological habitat relationship analogues, pol-len transport characteristics, and the overall pollen assem-blage, we propose that both Picea and Sabina pollen were transported by the river from the mountains at the upper reaches of the Shiyang River, and that the assemblage is more indicative of changes in upland vegetation than of local conditions near the section. This interpretation is supported by pollen data derived from surface samples, water samples, and riverbed samples. Using a moisture indicator (the Picea to Sabina ratio) and calculated pollen concentrations, we identify a series of

Structural Design of High-precision Positioning System in Weak Signal Environment Based on UWB and IMU Fusion

Aiming at the problem that indoor positioning technology based on wireless ultra-wideband pulse technology is susceptible to non-line-of-sight effects and multipath effects in confined spaces and weak signal environments,a high-precision positioning system based on UWB and IMU in a confined environment is designed.The STM32 chip is used as the main control,and the data information of IMU and UWB is fused by the fusion filtering algorithm.Finally,the real-time information of the positioning is transmitted to the host computer and the cloud.The experimental results show that the positioning accuracy and positioning stability of the system have been improved in the non-line-of-sight case of closed environment.The system has high positioning accuracy in a closed environment,and the components used are consumer-grade,which has strong practicability.

Two-step compressed acquisition method for Doppler frequency and Doppler rate estimation in high-dynamic and weak signal environments

To acquire global navigation satellite system(GNSS)signals means four-dimension acquisition of bit transition,Doppler frequency,Doppler rate,and code phase in high-dynamic and weak signal environments,which needs a high computational cost.To reduce the computations,this paper proposes a twostep compressed acquisition method(TCAM)for the post-correlation signal parameters estimation.Compared with the fast Fourier transform(FFT)based methods,TCAM uses fewer frequency search points.In this way,the proposed method reduces complex multiplications,and uses real multiplications instead of improving the accuracy of the Doppler frequency and the Doppler rate.Furthermore,the differential process between two adjacent milliseconds is used for avoiding the impact of bit transition and the Doppler frequency on the integration peak.The results demonstrate that due to the reduction of complex multiplications,the computational cost of TCAM is lower than that of the FFT based method under the same signal to noise ratio(SNR).

Methods for Analysis of Protein Glutathionylation and their Application to Photosynthetic Organisms

Protein S-glutathionylation, the reversible formation of a mixed-disulfide between glutathione and protein thiols, is involved in protection of protein cysteines from irreversible oxidation, but also in protein redox regulation. Recent studies have implicated S-glutathionylation as a cellular response to oxidative/nitrosative stress, likely playing an important role in signaling. Considering the potential importance of glutathionylation, a number of methods have been developed for identifying proteins undergoing glutathionylation. These methods, ranging from analysis of purified proteins in vitro to large-scale proteomic analyses in vivo, allowed identification of nearly 200 targets in mammals. By contrast, the number of known glutathionylated proteins is more limited in photosynthetic organisms, although they are severely exposed to oxidative stress. The aim of this review is to detail the methods available for identification and analysis of glutathionylated proteins in vivo and in vitro. The advantages and drawbacks of each technique will be discussed as well as their application to photosynthetic organisms. Furthermore, an overview of known glutathionylated proteins in photosynthetic organisms is provided and the physiological importance of this post-translational modification is discussed.

An Approach to Active Learning for Classifier Systems

In this paper, the active learning mechanism is proposed to beused in classifier systems to cope with complex problems: an intelligent agent leavesits own signals in the environment and later collects and employs them to assistits learning process. Principles and components of the mechanism are outlined,followed by the introduction of its preliminary implementation in an actual system.An experiment with the system in a dynamic problem is then introduced, togetherwith discussions over its results. The paper is concluded by pointing out somepossible improvements that can be made to the proposed framework.

Transcriptional regulation of oil biosynthesis inseed plants: Current understanding, applications,and perspectives

Plants produce and accumulate triacylglycerol(TAG)in their seeds as an energy reservoir to support the processes of seed germination and seedling development.Plant seed oils are vital not only for the human diet but also as renewable feedstocks for industrial use.TAG biosynthesis consists of two major steps:de novo fatty acid biosynthesis in the plastids and TAG assembly in the endoplasmic reticulum.The latest advances in unraveling transcriptional regulation have shed light on the molecular mechanisms of plant oil biosynthesis.We summarize recent progress in understanding the regulatory mechanisms of wellcharacterized and newly discovered transcription factors and other types of regulators that control plant fatty acid biosynthesis.The emerging picture shows that plant oil biosynthesis responds to developmental and environmental cues that stimulate a network of interacting transcriptional activators and repressors,which in turn fine-tune the spatiotemporal regulation of the pathway genes.