Trehalose is a non-reducing disaccharide, and widespread in nature. It is a typical stress metabolite that can protect macromolecules such as proteins in organisms under extreme conditions. Therefore, trehalose has be...Trehalose is a non-reducing disaccharide, and widespread in nature. It is a typical stress metabolite that can protect macromolecules such as proteins in organisms under extreme conditions. Therefore, trehalose has been widely used in food, medicine and cosmetics. Trehalose is extracted from yeast or synthesized by chemical method. Due to the high cost of traditional methods, trehalose is mainly produced by enzymatic methods. There are mainly three pathways: TPS/TPP, TreY/TreZ and TreS. These enzymatic systems are expressed mainly through heterologous expression.展开更多
Monoacylglycerols(MAGs) and diacylglycerols(DAGs) are partial glycerides widely used in food industry. They are safe and non-toxic food emulsifiers, especially for MAGs. MAGs account for approximately 75% of the total...Monoacylglycerols(MAGs) and diacylglycerols(DAGs) are partial glycerides widely used in food industry. They are safe and non-toxic food emulsifiers, especially for MAGs. MAGs account for approximately 75% of the total emulsifiers in food industry worldwide. DAGs are recognized as functional cooking oils, they can suppress body fat accumulation and postprandial serum triacylglycerols(TAGs) level. The traditional production of MAGs and DAGs is based on the chemical method, which requires high reaction temperature usually up to 200–260 ℃. Such high temperature is not suitable for oil containing heat sensitive polyunsaturated fatty acids. Enzymatic approach has been received increasing attentions. Enzymatic production of partial glycerides to replace chemical processes has been in industry, particularly for DAGs production as the products have been claimed as a functional and nutritional oil. Enzyme technology for the processing of oils and fats has been moved to industry step by step and case by case during the last 20 years. More and more applications are particularly moving into bulky oils and fats processing. At the same time, the cost of enzymes as a commercial product is reducing steadily. This review summarized the recent 15 years advances on the the enzymatic preparation of MAGs and DAGs. The critical process parameters under different reaction routes were presented and emphasized. The reaction media not only increased the homogeneity of the reaction system, but also shifted the reaction equilibrium towards the target product generation, and this part was stated in detail. In addition, the patent evaluation was included, and the application of MAGs and DAGs was covered.展开更多
基金Supported by National Nature Science Foundation of China(No.32001632)。
文摘Trehalose is a non-reducing disaccharide, and widespread in nature. It is a typical stress metabolite that can protect macromolecules such as proteins in organisms under extreme conditions. Therefore, trehalose has been widely used in food, medicine and cosmetics. Trehalose is extracted from yeast or synthesized by chemical method. Due to the high cost of traditional methods, trehalose is mainly produced by enzymatic methods. There are mainly three pathways: TPS/TPP, TreY/TreZ and TreS. These enzymatic systems are expressed mainly through heterologous expression.
基金supported by the National Natural Science Foundation of China(31772000).
文摘Monoacylglycerols(MAGs) and diacylglycerols(DAGs) are partial glycerides widely used in food industry. They are safe and non-toxic food emulsifiers, especially for MAGs. MAGs account for approximately 75% of the total emulsifiers in food industry worldwide. DAGs are recognized as functional cooking oils, they can suppress body fat accumulation and postprandial serum triacylglycerols(TAGs) level. The traditional production of MAGs and DAGs is based on the chemical method, which requires high reaction temperature usually up to 200–260 ℃. Such high temperature is not suitable for oil containing heat sensitive polyunsaturated fatty acids. Enzymatic approach has been received increasing attentions. Enzymatic production of partial glycerides to replace chemical processes has been in industry, particularly for DAGs production as the products have been claimed as a functional and nutritional oil. Enzyme technology for the processing of oils and fats has been moved to industry step by step and case by case during the last 20 years. More and more applications are particularly moving into bulky oils and fats processing. At the same time, the cost of enzymes as a commercial product is reducing steadily. This review summarized the recent 15 years advances on the the enzymatic preparation of MAGs and DAGs. The critical process parameters under different reaction routes were presented and emphasized. The reaction media not only increased the homogeneity of the reaction system, but also shifted the reaction equilibrium towards the target product generation, and this part was stated in detail. In addition, the patent evaluation was included, and the application of MAGs and DAGs was covered.
