Objective To investigate the effects of 3,4-dichloroaniline （3,4-DCA） on activities of testicle enzymes as biological markers in rats. Methods Fifty male rats were randomly divided into 5 groups （n=10）. One group ...Objective To investigate the effects of 3,4-dichloroaniline （3,4-DCA） on activities of testicle enzymes as biological markers in rats. Methods Fifty male rats were randomly divided into 5 groups （n=10）. One group was left untreated and used as a solvent control （administered orally by corn oil）, while the other 4 groups were treated with 3, 4-DCA. Corn oil was used as a solvent, and 3,4-DCA was diluted into tested concentrations （39, 81, 170, and 357 mg/kg）. All the groups orally administered 3,4-DCA or corn oil, once a day for 4 weeks. The testicle tissue was homogenized in a 0.1 mol/L potassium phosphate buffer （0.1 mol/L, pH 7.2）. The crude homogenate was centrifuged at 6000 rpm for 5 min at 4 ~C. The supernatant obtained was used as an enzyme extract for determination of the enzyme activities. Results Compared with the control, the activities of ALP, ACP, and SDH were increased significantly at a lower level of 3,4-DCA, and decreased at a higher level of 3, 4-DCA, whreas the activities of LDH, LDH-X, and G6PDH were inhibited significantly with the increased 3,4-DCA concentration. Organ coefficient ＂organ weight/total body weight × 100＂ of testis, liver, and spleen increased significantly with the increased 3,4-DCA concentration. These results suggest that 3,4-DCA toxicity to the male reproductive system was associated with the activities of testicular enzymes which are the sensitive biochemical endpoints reflecting 3,4-DCA toxicity to the male reproductive system. Conclusion 3,4-DCA has toxicity to the reproductive system in male rats.展开更多
The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. Th...The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were cosistent with their functions. In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae. Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma cells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five marker enzymes were discussed.展开更多
基金supported by the Natural Science Foundation of Beijing(No.7063071).
文摘Objective To investigate the effects of 3,4-dichloroaniline （3,4-DCA） on activities of testicle enzymes as biological markers in rats. Methods Fifty male rats were randomly divided into 5 groups （n=10）. One group was left untreated and used as a solvent control （administered orally by corn oil）, while the other 4 groups were treated with 3, 4-DCA. Corn oil was used as a solvent, and 3,4-DCA was diluted into tested concentrations （39, 81, 170, and 357 mg/kg）. All the groups orally administered 3,4-DCA or corn oil, once a day for 4 weeks. The testicle tissue was homogenized in a 0.1 mol/L potassium phosphate buffer （0.1 mol/L, pH 7.2）. The crude homogenate was centrifuged at 6000 rpm for 5 min at 4 ~C. The supernatant obtained was used as an enzyme extract for determination of the enzyme activities. Results Compared with the control, the activities of ALP, ACP, and SDH were increased significantly at a lower level of 3,4-DCA, and decreased at a higher level of 3, 4-DCA, whreas the activities of LDH, LDH-X, and G6PDH were inhibited significantly with the increased 3,4-DCA concentration. Organ coefficient ＂organ weight/total body weight × 100＂ of testis, liver, and spleen increased significantly with the increased 3,4-DCA concentration. These results suggest that 3,4-DCA toxicity to the male reproductive system was associated with the activities of testicular enzymes which are the sensitive biochemical endpoints reflecting 3,4-DCA toxicity to the male reproductive system. Conclusion 3,4-DCA has toxicity to the reproductive system in male rats.
基金This study is supported by the national natural science fund of China
文摘The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were cosistent with their functions. In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae. Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma cells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five marker enzymes were discussed.
