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Color Fastness of Dyed Raw Linen Cloth Modified with Enzymes
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作者 Bernava Aina Borisova Anna +1 位作者 Strazds Guntis Reihmane Skaidrite 《Journal of Chemistry and Chemical Engineering》 2014年第7期686-691,共6页
Green thinking looks to develop alternatives with higher environmental advantage that traditional materials or processes. The present experimental studies are directed to investigation of dyeing the linen fabric prepa... Green thinking looks to develop alternatives with higher environmental advantage that traditional materials or processes. The present experimental studies are directed to investigation of dyeing the linen fabric prepared with modification with enzymes. In the research, 1% and 2% solution of cellulase enzyme Beizym ENC-SB (garment auxiliary of enzymes mixture of CHT R. BEITLICH GmbH/BEZEMA AG) and pectinase Beisol PRO (enzymes mixture for cleaning cellulose fibers of CHT R. BE1TLICH GmbH) enzyme solutions were used. The main objective of this work is focus on results studies of enzyme influence on dyeing of flax fabric with direct Solphenyl dye triade Blue FGLE (economical, fairly good light fast blue, main trichromatic component for medium shades with high wash fastness), Scarlet BNLE (economical scarlet, main trichromatic component for medium to dark shades) 200%, and Yellow GLE (very bright neutral yellow, main trichromatic component for high wash fastness requirements). The dye-bath exhaustion, color fastness to rubbing and washing were estimated. The color parameters before and after tests were calculated. 展开更多
关键词 Raw linen cloth PECTINASE cellulase enzymes modification direct dye color fastness.
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Modification of ubiquitin C-terminal hydrolase L1 by reactive lipid species: role in neural regeneration and diseases of aging
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作者 Steven H.Graham 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第6期908-909,共2页
Role of ubiquitin C-terminal hydrolase L1(UCHL1)in brain function:Ubiquitin is used by a variety of cellular systems to tag proteins for transport to various organelles.There are a number of enzymes in the ubiquiti... Role of ubiquitin C-terminal hydrolase L1(UCHL1)in brain function:Ubiquitin is used by a variety of cellular systems to tag proteins for transport to various organelles.There are a number of enzymes in the ubiquitin-proteasome pathway(UPP)that tag abnormally folded proteins with ubiquitin for transport to the proteasome for degradation. 展开更多
关键词 ubiquitin proteasome regeneration Ubiquitin hydrolase modification synaptic neuronal lipid enzymes
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Enzyme engineering in microbial biosynthesis of terpenoids:progress and perspectives
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作者 Amna Bibi SU Liqiu +1 位作者 DAI Zongjie WANG Qinhong 《生物工程学报》 CAS CSCD 北大核心 2024年第8期2473-2488,共16页
Terpenoids,known for their structural and functional diversity,are highly valued,especially in food,cosmetics,and cleaning products.Microbial biosynthesis has emerged as a sustainable and environmentally friendly appr... Terpenoids,known for their structural and functional diversity,are highly valued,especially in food,cosmetics,and cleaning products.Microbial biosynthesis has emerged as a sustainable and environmentally friendly approach for the production of terpenoids.However,the natural enzymes involved in the synthesis of terpenoids have problems such as low activity,poor specificity,and insufficient stability,which limit the biosynthesis efficiency.Enzyme engineering plays a pivotal role in the microbial synthesis of terpenoids.By modifying the structures and functions of key enzymes,researchers have significantly improved the catalytic activity,specificity,and stability of enzymes related to terpenoid synthesis,providing strong support for the sustainable production of terpenoids.This article reviews the strategies for the modification of key enzymes in microbial synthesis of terpenoids,including improving enzyme activity and stability,changing specificity,and promoting mass transfer through multi-enzyme collaboration.Additionally,this article looks forward to the challenges and development directions of enzyme engineering in the microbial synthesis of terpenoids. 展开更多
关键词 TERPENOIDS microbial synthesis enzyme engineering enzyme modification terpene synthases
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Antibody-Like Phosphorylation Sites in Focus of Statistically Based Bilingual Approach 被引量:2
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作者 Jaroslav Kubrycht Karel Sigler +1 位作者 Pavel Souček Jiří Hudeček 《Computational Molecular Bioscience》 2016年第1期1-22,共22页
In accordance with previous reports, the sequences related to phosporylated protein segments occur in conserved variable domains of immunoglobulins including first of all certain N-terminally located segments. Consequ... In accordance with previous reports, the sequences related to phosporylated protein segments occur in conserved variable domains of immunoglobulins including first of all certain N-terminally located segments. Consequently, we look here for the sequences 1) composing human and mouse proteins different from antigen receptors, 2) identical with or highly similar to nucleotide sequence representatives of conserved variable immunoglobulin segments and 3) identical with or closely related to phosphorylation sites. More precisely, we searched for the corresponding actual pairs of DNA and protein sequence segments using five-step bilingual approach employing among others a) different types of BLAST searches, b) two in-principle-different machine-learning methods predicting phosphorylated sites and c) two large databases recording existing phosphorylation sites. The approach identified seven existing phosphorylation sites and thirty-seven related human and mouse segments achieving limits for several predictions or phylogenic parameters. Mostly serines phosporylated with ataxia-telangiectasia-related kinase (involved in regulation of DNA-double-strand-break repair) were indicated or predicted in this study. Hypermutation motifs, located in effective positions of the selected sequence segments, occurred significantly less frequently in transcribed than non-transcribed DNA strands suggesting thus the incidence of mutation events. In addition, marked differences between the numbers and proportions of human and mouse cancer-related sequence items were found in different steps of selection process. The possible role of hypermutation changes within the selected segments and the observed structural relationships are discussed here with respect to DNA damage, carcinogenesis, cancer vaccination, ageing and evolution. Taken together, our data represent additional and sometimes perhaps complementary information to the existing databases of empirically proven phosphorylation sites or pathogenically important spots. 展开更多
关键词 Ataxia Telangiectasia-Mutated-Protein (i.e. Kinase ATM Whose Pathogenic Mutation Is Responsible for Early Death of People) Complementarity Determining Region 1 (of Immunoglobulins i.e. CDR1 or Hypervariable Region 1) Database (of Functional Structures) Hypermutation (i.e. Mutation of DNA Sequences Mediated by enzymes) Immunoglobulin (i.e. Ig or Antibody) Phosphorylation (enzyme Mediated modification Concerns Here Mostly Protein Sequences)
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