Establishment of a TLC Identification Method for Ensete wilsonii

[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.

Identification of Qishen Oral Solution with TLC and HPLC

[Objective] This study aimed to establish the standards for quality control of Qishen oral solution. [Method] Astragalus membranaceus, Atractylodes macro- cephala Koidz. and Glycyrrhiza uralensis in the preparation were identified by using thin layer chromatography （TLC）, and Codonopsis pilosula was identified by using high-performance liquid chromatography （HPLC）. [Result] By using the developed TLC system, experimental solution and reference solution showed clear spots, while negative control presented no interference. By using the developed HPLC system, the chromatographic peak of Iobetyolin was detected in experimental solution. [Con- clusion] The developed TLC and HPLC systems presented high specificity and good repeatability for identification of these four components and could be used in the quality control of Qishen oral solution.

微量石油沥青族组成 TLC/FID 分析方法及应用

关于重质油(包括石油沥青)的族组成分析一般采用液固色谱方法。然而这种柱色谱法操作过程繁琐,样品用量大,在分离过程中又存在不同程度的不可逆吸附。TLC/FID方法是根据现行沥青族组成分离过程的局限性,采用薄层色谱(TLC)与氢火焰离子化检测器(FID)相结合,以硅胶或氧化铝涂制于 TLC 烧结棒上,点样和色谱展开,在棒架上以恒定的速度移动通过检测器火焰,各组分被自动扫描。样品从分离到检定仅需要一小时,并可同时进行十个样品的分析,定量线性关系良好,准确度在色谱允许误差范围内。TLC/FID 分离色谱图见图1。

串行双通道DAC TLC5618A与DSP的接口技术

介绍了双通道DAC TLC5618A与DSP芯片TMS320C203接口的硬件设计和软件编程,该方法也适用于DSP与其它具有SPI总线的DAC器件。

串行双通道DAC TLC5618A与DSP的接口技术

TLC5618A和TMS320C203分别是TI公司生产的2位DAC和DSP芯片 ,文中在介绍了TLC5618A的功能和原理的基础上 ,对双通道的DACTLC5618A与DSP芯片TMS320C203进行接口的硬件设计和软件编程进行了详细描述 ,该设计方法也适用于DSP与其它具有SPI总线的DAC器件。

TI TLC698348x16共阴矩阵LED显示器驱动器解决方案

TI公司的TLC6983是高度集成的共阳48x16矩阵LED显示器驱动器,具有48个恒流电流源和16个扫描FET.单个TLC6983能驱动16x16 RGB LED象素,而堆栈两个TLC6983能驱动32x32 RGB LED象素.为了得到低功耗,器件支持独立电源,用于给共阴极结构的红绿蓝LED供电.此外,TLC6983的工作功率由于超低工作电压(Vcc低至2.5V)和超低工作电流(Icc低至3.9mA)而大大地降低.VCC电压范围为2.5V-5.5V,VR/G/B电压范围为2.5V-5.5V,48个电流源通路从0.2 mA到20 mA,通路间的精确度为±0.5%(典型),±2%(最大值),器件间的精确度为±0.5%(典型),±2%(最大值).IOUT=5mA时的拐点电压为0.26V(最大值).

Determination of Moisture, Ash, Extract Content and TLC identification of Tibetan Medicinal Material Dracocephalum tanguticum Maxim.

[Objectives] The moisture, ash and extract content of Dracocephalum tanguticum Maxim. were measured.[Methods] The moisture, total ash, acid-insoluble ash and extract content of D. tanguticum Maxim collected from 16 producing areas were detected by using the methods in Chinese Pharmacopoeia (2015 edition, volume I), and D. tanguticum Maxim. was identified by thin layer chromatography in Chinese Pharmacopoeia (2015 edition, volume I).[Results] The moisture content of D. tanguticum Maxim collected from 16 producing areas ranged from 8.68% to 10.36%, averaging 9.28%. The content of total ash was between 7.21% and 11.60%, averaging 9.89%. The content of acid-insoluble ash was 0.21%-2.71%, averaging 1.51%. The extract content (water-soluble) was 26.67%-42.91%, averaging 32.65%. According to the results of TLC identification, the 16 samples and reference substance had the same characteristic spots at the corresponding positions.[Conclusions] It was recommended that the moisture, total ash and acid-insolue ash content of D. tanguticum Maxim were not be more than 11%, 12%, and 3% respectively, while the extract content was not less than 26%. It provides reference for filling the gaps in the quality standards of D. tanguticum Maxim.

Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method

Objective:To develop and validate an image analysis method for quantitative analysis ofγ-oryzanol in cold pressed rice bran oil.Methods:TLC-densitometric and TLC-image analysis methods were developed,validated,and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.The results obtained by these two different quantification methods were compared by paired t-test.Results:Both assays provided good linearity,accuracy,reproducibility and selectivity for determination of γ-oryzanol.Conclusions:The TLC-densitomelric and TLC-image analysis methods providett a similar reproducibility,accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil.A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods.As both methods were found to be equal,they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.

Detection of Berberine Hydrochloride in Jin Weng Zhili Particle Using TLC and HPLC

[Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography and high performance liquid chromatography were used respectively for qualitative and quantitative identification of berberine hydrochloride contained in Jin Weng Zhili Particle by improving the conditions of extraction and purification. [Results]After optimizing TLC conditions of Jin Weng Zhili Particle,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,and precision was high. Under the conditions of mobile phase constituted by acetonitrile-water-glacial acetic acid-triethylamine( 28∶ 70∶ 1∶ 1),detection wavelength of 265 nm,flow rate of 1. 0 m L min^(-1)and column temperature of 35℃,the resolution of berberine hydrochloride was good; the injection volume in the range of 3. 15-50 μg· m L^(-1)had good linear relationship with the peak area( R^2= 0. 998); the average sample recovery rate(n=6) was 5. 672%,and RSD was 0. 864%; the content of berberine hydrochloride contained in Jin Weng Zhili Particle was 4. 023 mg. [Conclusions]The method was of good feasibility,reproducibility and specificity,and could be used for qualitative and quantitative detection of berberine hydrochloride contained in Jin Weng Zhili Particle.

Identification of Gardenoside and Sarsasapogenin from Qingwen Baidu Granules by TLC

[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.

Identification of Coptis chinensis and Forsythia sus-pensa from Qingwen Baidu Granules by TLC

[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.

串行12位A/D TLC2543及其应用

TLC2543是采用CMOS技术的12位开关电容逐次逼近式模数转换器,它是依靠初始的电荷有电容器间被重新分配的原理来进行模数转换的。有11个模入通道,带自动采样保护,转换时间10μs。有3个输入控制端:片选(CS),串行输入/输出时钟((I/O CLOCK)和串行数据输入端,还有一个串有(?)态数据输出端。不需要其他元器件,可直接和单片机连接,接口电路非常简单。该芯片是一种高速高精度,接口简单,价格低廉的12位串行A/D器件,很适合于单片机测控系统。

PEONIES: Comparative Study by Anatomy and TLC of Three Traditional Chinese Medicinal Plants

Anatomical and TLC study of three Chinese peonies were conducted to make a comparative analysis. Peonies (Paeonia suffruticosa (tree peony), Paeonia lactiflora (Chinese peony) and Paeonia veitchii (Chinese peony)) are traditionally used on the Qinghai-Tibet Plateau in China. Recent studies have shown that the peonies have different pharmacological activities and clinical applications. To distinguish these three species of peonies and ensure the safety and effectiveness in their use, the microscopic characteristics and chromatographic profile of their roots and the corresponding powder were studied. Plant materials sectioned and stained and the raw powder were studied with an optical microscope using standard techniques in microscopy. The results of microscopic features and TLC were described and illustrated. The three species have different microscopic characteristics and TLC profiles, which allow us to distinguish them. In fact, with the help of features semi-quantitative and qualitative, an identification key was developed in our work and illustrated with photos and a table. The aim of our work was to show that the optical microscopy and related techniques provide a achievable practicality, which can be applied without ambiguity to the authentication of species peonies.

Study on TLC Differentiation for Chaige Changyuan Mixture

[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development conditions,the color development inspection method and other factors were investigated for thin-layer chromatography.The three main medicines of Scutellariae Radix,Peucedani Radix and Puerariae Radix were qualitatively identified.[Results]The TLC spots of Scutellariae Radix,Peucedani Radix and Puerariae Radix could be identified well by TLC,and corresponding negative control had no interference.[Conclusions]The method is simple,available and reproducible,and can be used for the quality control of Chaige Changyuan Mixture.

Screening of TLC Identification of Forsythin in Jin Weng Zhi Li Granule

[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.

Improvement of TLC Identification of Matrine in Jin Weng Zhi Li Granule

[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.

DETERMINATION OF POLYDATIN IN POLYGONUM CUSPIDTUM SIEB. ET ZUCC. BY TLC-FLUORESCENCE SPECTROPHOTOMETRY

Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with chloroform-acetone-formic acid-water (4∶4∶0.5∶0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284 nm and 384 nm, respectively. Results The linear regression equation of the calibration graph was y=7.02179x+4.5143, a linear regression correlative coefficient r=0.9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs.

读取性能稳定 金士顿UV400 240GB TLC SSD

在入门级SSD市场上，各家厂商已经是“刺刀见红”，金土顿同样也没有落后，金士顿UV400（以下简称UV400）系列便是他们派出的先头部队，我们来看—下这款UV400 240GB的战斗力究竟如何。

闪迪至尊高速2代240GB固态硬盘 TLC新势力

尽管固态硬盘的价格在近一年来已经有较大幅度的下跌，但像闪迪、浦科特、英特尔等固态硬盘中主力品牌的240GB-256GB产品（采用MLC颗粒）价格基本上都在1100元～1400元左右。因此为了满足预算并不宽裕的消费者，近期闪迪特别推出了一款低价固态硬盘——至尊高速2代，其240GB产品售价仅799元。