粗齿冷水花中Epihernandulcin的含量测定

目的:建立测定粗齿冷水花中Epihernandulcin含量的方法。方法:采用高效液相色谱法(HPLC)测定粗齿冷水花中Epihernandulcin含量,采用ECOSIL ODS-3反相色谱柱(250×4.6mm,5μm),以乙腈-0.2%磷酸溶液(65:35)为流动相进行等度洗脱,检测波长为240nm,流速为1m L/min,柱温为室温。结果:Epihernandulcin在0.114-0.683μg范围内呈良好线性关系(r=0.9991),平均回收率为99.50%,RSD=2.18%(n=9)。结论:该法简单,可行,重现性好,适用于粗齿冷水花中Epihernandulcin的含量测定。

A Study on the Chemical Constituents of Pilea sinofasciata C. J.Chen

[Objectives] To study the chemical constituents of Pilea sinofasciata C. J. Chen. [Methods] Column chromatography,such as silica gel,Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS,1H NMR and^(13)C NMR and physical constants were used to elucidate their structure. [Results]Ninteen compounds were isolated from 95% ethanol extracts of Pilea sinofasciata C. J. Chen,including α-tocopherol(1),stigmasterol(2),epihernandulcin(3),hernandulcin(4),benzoic acid(5),ethyl linolenate(6),ethyl hexadecanoate(7),α-amyrin(8),palmitic acid(9),behenic acid(10),adenosine(11),indole-3-carboxylic acid(12),protocatechuate(13),gallic acid(14),betulinic acid(15),oleanolic acid(16),potassium nitrate(17),diosmetin7-O-β-D-glucopyranoside( 18),3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid(19). [Conclusions] All of the compounds were isolated from the plant for the first time.

粗齿冷水花的化学成分研究

目的研究粗齿冷水花Pilea sinofasciata的化学成分。方法采用硅胶柱色谱、Sephadex LH-20、制备液相等多种色谱技术进行化合物的分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果从粗齿冷水花的95%乙醇提取物中分离得到19个化合物,分别鉴定为α-生育酚(1)、豆甾醇(2)、epihernandulcin(3)、hernandulcin(4)、苯甲酸(5)、亚麻油酸乙酯(6)、十六酸乙酯(7)、α-香树精(8)、棕榈酸(9)、二十二烷酸(10)、腺苷(11)、3-吲哚甲酸(12)、原儿茶酸(13)、没食子酸(14)、白桦脂酸(15)、齐墩果酸(16)、硝酸钾(17)、香叶木素-7-O-β-D-葡萄糖苷(18)、3-O-β-D-吡喃木糖基(1→2)-β-D-吡喃葡萄糖-齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷(19)。结论所有化合物均为首次从该植物中分离得到。