Objectives To investigate the effects of epoxy chloropropan on the expression of matrix metalloproteinases-9 （MMP-9）in creating tissue engineered heart valves（TEHV）,on the tissue structures of TEHV,and to study th...Objectives To investigate the effects of epoxy chloropropan on the expression of matrix metalloproteinases-9 （MMP-9）in creating tissue engineered heart valves（TEHV）,on the tissue structures of TEHV,and to study the effects of epoxy chloropropan on the calcification of TEHV.Methods The porcine aortic valve leaflets were digested and decellularized by using detergent and trypsin.Those treated with 0.3% glutaraldehyde for 48 hours were the control group;those treated with 3% epoxy choloropropan for 24 hours were the experimental group.The cultured human bone marrow mesenchymal stem cells（hBMSCs）were seeded onto the decellularized scaffolds of TEHV.The histological studies were done with pathological sections and scanning electron microscopy and reverse transcriptase-polymerase chain reaction（RT-PCR）were used to detect the expression of MMP-9.Results In the experimental group.the histology showed that the BMSCs grew well into the pores and formed a confluent layer in decellularized scaffolds;RT-PCR indicated significantly attenuated expressions of MMP-9,compared with the control（P〈0.05）.Conclusion The decellularized porcine aortic valves treated with 3% epoxy chloropropan may inhibit the expression of MMP-9;therefore epoxy chloropropan may prevent the calcification of tissue engineered heart valves.展开更多
首先用酸水解法制备纳米纤维素(NCC),通过接枝环氧氯丙烷(ECH)提高了其在环氧树脂(EP)中的分散性,将NCC-g-ECH以不同用量掺入到EP中测试其性能。结果表明,NCC-g-ECH用量为4%时,EP的冲击强度达到38 k J/m^2,弯曲强度为119.11 MPa,掺混后E...首先用酸水解法制备纳米纤维素(NCC),通过接枝环氧氯丙烷(ECH)提高了其在环氧树脂(EP)中的分散性,将NCC-g-ECH以不同用量掺入到EP中测试其性能。结果表明,NCC-g-ECH用量为4%时,EP的冲击强度达到38 k J/m^2,弯曲强度为119.11 MPa,掺混后EP的热稳定性也得到了增强。展开更多
文摘Objectives To investigate the effects of epoxy chloropropan on the expression of matrix metalloproteinases-9 （MMP-9）in creating tissue engineered heart valves（TEHV）,on the tissue structures of TEHV,and to study the effects of epoxy chloropropan on the calcification of TEHV.Methods The porcine aortic valve leaflets were digested and decellularized by using detergent and trypsin.Those treated with 0.3% glutaraldehyde for 48 hours were the control group;those treated with 3% epoxy choloropropan for 24 hours were the experimental group.The cultured human bone marrow mesenchymal stem cells（hBMSCs）were seeded onto the decellularized scaffolds of TEHV.The histological studies were done with pathological sections and scanning electron microscopy and reverse transcriptase-polymerase chain reaction（RT-PCR）were used to detect the expression of MMP-9.Results In the experimental group.the histology showed that the BMSCs grew well into the pores and formed a confluent layer in decellularized scaffolds;RT-PCR indicated significantly attenuated expressions of MMP-9,compared with the control（P〈0.05）.Conclusion The decellularized porcine aortic valves treated with 3% epoxy chloropropan may inhibit the expression of MMP-9;therefore epoxy chloropropan may prevent the calcification of tissue engineered heart valves.
文摘首先用酸水解法制备纳米纤维素(NCC),通过接枝环氧氯丙烷(ECH)提高了其在环氧树脂(EP)中的分散性,将NCC-g-ECH以不同用量掺入到EP中测试其性能。结果表明,NCC-g-ECH用量为4%时,EP的冲击强度达到38 k J/m^2,弯曲强度为119.11 MPa,掺混后EP的热稳定性也得到了增强。
