A RP-HPLC method was used for the determination of eprinomectin concentration in sheep plasma following i.v. and s.c. administration at a single dose of 0.2 mg kg 1. Eprinomectin in plasma within 2.5200 ng ml 1 rang...A RP-HPLC method was used for the determination of eprinomectin concentration in sheep plasma following i.v. and s.c. administration at a single dose of 0.2 mg kg 1. Eprinomectin in plasma within 2.5200 ng ml 1 ranges had a good linear relationship(R=0.9968).The average recovery of the method was 99.65±3.84%.The RSD% of within-day and between-day assays were less than 10 and 12%, respectively. The extract of plasma samples were loaded onto a C 18 catridge. After solvent exchange, the methanol eluate was derivatized via the addition of 1-methylimidazole and trifluoroacetic anhydride in acetonitrile. The fluorescent derivative was analyzed.The main pharmacokinetic parameters were as follows, for i.v. administration: T 1/2β=12.66±2.05 h, AUC 0t= 1.02 ± 0.3 mg h L 1, f c=0.13±0.05; for s.c. administration: T 1/2Ka= 4.42 ±1.04 h, C max=0.02±0.01 μg ml 1, T max= 15.36 ± 2.91 h, t 1/2K=26.22±9.04 h, AUC 0t= 1.19±0.37 mg h L 1. The results showed that eprinomectin was distributed widely and taken long time to eliminate in sheep after i.v. adminstration. When given subcutaneously, eprinomectin had better absorption and longer residue time in sheep.Eprinomectin was eliminated much slowly after s.c. adminstration compared with i.v. administration.展开更多
北京动物园因引进羊驼携带疥螨,并造成了数只羊驼患疥螨病死亡。在对该病例进行分析和确诊的基础上,对该群羊驼进行了防治,成功治愈了剩余的羊驼。本病例提示我们,疥螨是一种传染性、致病性很强的寄生虫病,在引进羊驼时需要采取有效的...北京动物园因引进羊驼携带疥螨,并造成了数只羊驼患疥螨病死亡。在对该病例进行分析和确诊的基础上,对该群羊驼进行了防治,成功治愈了剩余的羊驼。本病例提示我们,疥螨是一种传染性、致病性很强的寄生虫病,在引进羊驼时需要采取有效的隔离和防治措施,严防疥螨引入;羊驼有特殊的生理特点,使用药物防治羊驼疥螨病时,不能照搬其他动物疥螨病的用药方案。本病例的治疗方案：给羊驼的患部涂搽高效氯氰菊酯油剂,每次涂搽20～30 m L,每周涂搽2次;同时给羊驼皮下注射0.6 mg/kg的乙酰氨基阿维菌素注射剂,每周给药2次;连续给药1个月左右。以上方案有效治愈了羊驼的疥螨病,为今后我国羊驼疥螨病防治提供了参考。展开更多
文摘A RP-HPLC method was used for the determination of eprinomectin concentration in sheep plasma following i.v. and s.c. administration at a single dose of 0.2 mg kg 1. Eprinomectin in plasma within 2.5200 ng ml 1 ranges had a good linear relationship(R=0.9968).The average recovery of the method was 99.65±3.84%.The RSD% of within-day and between-day assays were less than 10 and 12%, respectively. The extract of plasma samples were loaded onto a C 18 catridge. After solvent exchange, the methanol eluate was derivatized via the addition of 1-methylimidazole and trifluoroacetic anhydride in acetonitrile. The fluorescent derivative was analyzed.The main pharmacokinetic parameters were as follows, for i.v. administration: T 1/2β=12.66±2.05 h, AUC 0t= 1.02 ± 0.3 mg h L 1, f c=0.13±0.05; for s.c. administration: T 1/2Ka= 4.42 ±1.04 h, C max=0.02±0.01 μg ml 1, T max= 15.36 ± 2.91 h, t 1/2K=26.22±9.04 h, AUC 0t= 1.19±0.37 mg h L 1. The results showed that eprinomectin was distributed widely and taken long time to eliminate in sheep after i.v. adminstration. When given subcutaneously, eprinomectin had better absorption and longer residue time in sheep.Eprinomectin was eliminated much slowly after s.c. adminstration compared with i.v. administration.
文摘北京动物园因引进羊驼携带疥螨,并造成了数只羊驼患疥螨病死亡。在对该病例进行分析和确诊的基础上,对该群羊驼进行了防治,成功治愈了剩余的羊驼。本病例提示我们,疥螨是一种传染性、致病性很强的寄生虫病,在引进羊驼时需要采取有效的隔离和防治措施,严防疥螨引入;羊驼有特殊的生理特点,使用药物防治羊驼疥螨病时,不能照搬其他动物疥螨病的用药方案。本病例的治疗方案：给羊驼的患部涂搽高效氯氰菊酯油剂,每次涂搽20～30 m L,每周涂搽2次;同时给羊驼皮下注射0.6 mg/kg的乙酰氨基阿维菌素注射剂,每周给药2次;连续给药1个月左右。以上方案有效治愈了羊驼的疥螨病,为今后我国羊驼疥螨病防治提供了参考。
