气候变化对灯盏花(Erigeron breviscapus)在中国潜在适生区的影响

为了探讨气候变化对灯盏花潜在生态适宜区的影响,基于灯盏花(Erigeron breviscapus)分布数据、气候、土壤和地形等环境因子,运用MaxEnt模型模拟灯盏花在中国的潜在分布,采用刀切法筛选出影响灯盏花潜在分布的关键环境因子,并借助于ArcGIS软件中的空间分析工具分析气候变化对我国灯盏花潜在适生区的影响。结果表明:MaxEnt模型能够极好地模拟灯盏花的潜在分布,气温年较差、年均降水量和海拔是影响灯盏花潜在分布的关键环境因子;与基准气候(1970—2000年)相比,可持续发展路径(SSP126)情景和中间发展路径(SSP245)情景下灯盏花高适生区面积分别减少了21229.17、16053.47 km^(2),仅传统发展路径(SSP585)情景下中高适生区面积共增加27749.03 km^(2),SSP126和SSP245情景下灯盏花适生区的质心均向东南方向移动,仅SSP585情景下灯盏花适生区的质心向东北方向移动。综上所述,各气候情景下灯盏花适生区均主要分布于我国西南地区,但SSP126和SSP245情景下灯盏花在我国的潜在适生区面积缩减,仅SSP585情景有利于我国灯盏花的分布。

短葶飞蓬(Erigeron breviscapus)的花部综合特征与繁育系统

通过野外观察，运用杂交指数、花粉．胚珠比、套袋实验等方法，对短葶飞蓬自然种群的开花状态及繁育系统进行研究。结果如下：短葶飞蓬具头状花序，由外围舌状花及中央的管状花构成。种群花期一般为4～7月份，一个花序的花期约为19～25d。管状花单花花期一般为7～10d，聚药雄蕊，先熟，药室内向开裂，成熟的花粉散于花药筒中。花开后花柱快速伸长，导致雄蕊伸出花冠，同时将药筒中的花粉粒“推”出药筒，形成花冠、药筒、柱头三者在空间上的分离。花序中不断有小花开放，同一花序内的小花间具相互传粉的机会。杂交指数≥4，判断繁育类型属于异交，部分自亲和，需要传粉者。P／O值约为1373，判断繁育类型属于兼性异交。套袋实验证明短葶飞蓬异花授粉，虫媒，蜂、蝶为主要传粉者。在自然状况下，短葶飞蓬结实率较低的原因可能是花粉竞争。此外，短葶飞蓬存在天然雄性不育植株，为遗传育种提供了材料。

Glycosides from Erigeron breviscapus

A new glycoside, gamma-pyranone-3-O-beta-D-[6'-(4'-hydroxy-3',5'-dimethyoxybenzoyl)]-glucopyranoside (erigeside D, 2), together with nine known compounds, viz., erigeside I (1), erigeside A (3), erigeside B (4), erigeside II (5), scopolin (6), icariside B-2 (7), blumenol C glucoside (8), (+)-syringaresinol O-beta-D-glucopyranoside (9) and scutellarein-7-O-beta-D-glucuronide methyl ester (10), was isolated from Erigeron breviscapus (Vant.) Hand.-Mazz. Their structures were established on the basis of spectral evidence. According to the beta-glucopyranosyl moiety in 7, the absolute configuration of 7 was determined by X-ray crystallographic analysis. Compounds 7, 8 and 9 were isolated for the first time from this genus, an compound 6 was first obtained from this plant.

灯盏细辛(Erigeron breviscapus)的含量测定方法研究

目的在分析现有方法局限性的基础上建立合理的灯盏细辛的含量测定方法。方法①高效液相色谱-二极管阵列检测法(HPLC-DAD):Hypersil ODS2(250×4.6mm,5μm)色谱柱,乙腈-水-磷酸(18∶82∶0.2)为流动相,流速0.8mL/m in,检测波长286nm,柱温40℃;②紫外分光光度法(UV)。结果①HPLC-DAD分析指出灯盏细辛的主要成分为咖啡酰衍生物和黄酮;②提出测定野黄芩苷的HPLC方法和以咖啡酸为对照品测定总酚的UV方法。结论将测定野黄芩苷和测定总酚相结合。

Phenolic Compounds from Erigeron breviscapus (Compositae)

Five caffeoyl conjugates were isolated from the ethyl acetate soluble fraction of Erigeron breviscapus (Van.) Hand_Mazz for the first time. Along with them, nine other chemical constituents were also identified. One new caffeoyl conjugate, erigoster A (1) with a novel basic skeleton was mainly assigned by the using a combination of two_dimensional NMR techniques.

A New Glycoside from Erigeron Breviscapus

Erigeside I was isolated from Erigeron breviscapus. The structure elucidation and H-1. C-13 NMR. assignments were achieved by spectroscopic method.

Extraction(DSX) from Erigeron breviscapus modulates outward potassium currents in rat retinal ganglion cells

AIMTo investigate the effect of DSX, an active component extracted from Erigeron breviscapus, on the voltage-gated outward K+ channel currents in rat retinal ganglion cells (RGCs) by using electrophysiological method, and to explore the possible mechanisms of DSX on optic nerve protection.METHODSOutward K+ currents were recorded by using whole-cell patch-clamp techniques on acutely isolated rat RGCs. Outward K+ currents were induced by a series of depolarizing voltage pulses from a holding potential of -70 mV to +20 mV in an increment of 10 mV.RESULTSExtracellular application of DSX voltage-dependently suppressed both the steady-state and peak current amplitudes of outward K+ currents in rat RGCs. Furthermore, DSX reversibly and dose-dependently inhibited the amplitudes of outward K+ currents of the cells. At +20 mV membrane potential DSX at the concentrations of 0.02 g/L and 0.05 g/L showed no significant effects on the currents. In contrast, DSX at higher concentrations (0.1 g/L, 0.2 g/L and 0.5 g/L) significantly suppressed the current amplitudes.CONCLUSIONThese results suggest that DSX reversibly and dose-dependently suppress outward K+ channel currents in rat RGCs, which may be one of the possible mechanisms underlying Erigeron breviscapus prevents vision loss and RGC damage caused by glaucoma.

Effect on multifocal electroretinogram in persistently elevated intraocular pressure by erigeron breviscapus extract

AIM: To observe the effect on multifocal electroretinogram (mfERG) in persistently elevated intraocular pressure (TOP) by erigeron breviscapus extract (also named Dengzhanhua in Chinese) in rat models. METHODS: The rat models with persistently elevated TOP were established by the method of Akira. Then, erigeron breviscapus extract was given for one month to observe the effect on mfERG in persistently elevated TOP in rats. RESULTS: As elevated TOP went on, the mfERG changes were mainly in weaken of reaction density with progressive development. After intervention of erigeron breviscapus extract, the total peak latency of 131 wave had recovered to some extent and the difference was significant when compared with control group (P <0.05); the total response density and P1 wave response density in second circle had risen noticeably, which had significant differences than those of control group (P <0.05). CONCLUSION: Erigeron breviscapus extract can improve the impaired visual function of persistently elevated TOP in rats, suggesting that this extract is the effective part of erigeron breviscapus for optic neuroprotection.

The Effect of Erigeron Breviscapus on Proliferation of Pulmonary Artery Smooth Muscle Cells in Hypoxic Porcines

In order to study the effect of Erigeron Breviscapus (EB) on proliferation of pulmonary artery smooth muscle cells (PASMC) in hypoxic porcines, immunohistochemical and MTT methods were employed to measure the proliferation of PASMC. It was found that the proliferation of PASMC in porcines was obvious, and the expression of proliferating cell nuclear antigen (PCNA) was significantly high within 48 h after exposure to hypoxia. The EB could inhibit the proliferation and the expression of PCNA in PASMC under hypoxia, but it had no effect on the proliferation and expression of PCNA in PASMC under normal condition. The EB could inhibit the proliferation and the expression of PCNA in PASMC induced by phorbol 12-myristate 13-acetate (PMA), an agonist of PKC in normal and hypoxic conditions. It was concluded that the hypoxia could enhance the proliferation and expression of PCNA in PASMC. The EB can inhibit the proliferation and expression of PCNA in PASMC under hypoxia through PKC-signal way. The EB may be used in treating the pulmonary hypertension by inhibiting the proliferation of PASMC and the pulmonary vascular remodeling.

Effects of erigeron breviscapus (Vant.) Hand-Mazz pretreatment on pathology and oxyradical level following spinal cord ischemia-reperfusion injury in rabbits

Objective To investigate the effects of erigeron breviscapus (Vant.) Hand-Mazz (erigeron breviscapus) pretreatment on pathology and oxyradical level in the spinal cord after ischemia-reperfusion (I/R) injury in rabbits. Methods A total of 40 New Zealand white rabbits were randomly divided into three groups: sham-operation group with 10 rabbits treated with only abdominal aorta exposure without occlusion,control group with 15 rabbits that underwent ischemia for 50 minutes and treated with matched saline,and experimental group with 15 rabbits that underwent ischemia for 50 minutes and treated with erigeron breviscapus (9mg/kg) injection before ischemia. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the spinal cord were examined at 6 and 24 hours after I/R,respectively. The morphological changes and the number of the spinal cord anterior horn motor neurons were observed and counted under the light microscope and electron microscope,respectively. Results The level of MDA was markedly decreased and SOD activity was increased in the experimental group compared with those in the control group (P<0.01). Compared with that in the control group,the number of motor neurons in the experimental group significantly increased at 24h after I/R (P<0.01) and the morphous of the motor neurons improved. Conclusion Erigeron breviscapus can reduce oxyradical production and the apoptosis of nerve cells,and protect nerve tissue structure and function after spinal cord I/R.

Advances in ethnopharmacology,phytochemistry and pharmacology of Erigerontis Herba and its Chinese medicine prescriptions

Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away cold.It has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and toothache.This review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of EH.Relevant information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic databases.EH is a member of Compositae family and is mainly grown in southern China.Traditional Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving pain.By now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other compounds.EH extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative diseases.Scutellarin,the major active compound of EH,has been used as a quality marker.And no obvious toxicity of EH has been reported.According to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine fields.While several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain elusive.To fully exploit the medicinal potential of EH,further studies on understanding the effective material basis and mechanisms are warranted.

灯盏菊(Erigeron breviscapus (vant.) Hand-mazz)单株组培工厂化生产种苗技术研究

筛选了药用植物灯盏菊愈伤组织诱导、增殖及生根培养基配方 ,首次用单一灯盏菊植株外植体进行诱导产生的愈伤组织转移诱导出灯盏菊芽 .完成了该种植物组培苗生根和入土栽培的整套技术研究 ,为灯盏菊的人工栽培和育种提供了一个新的途径 .

酶法制备野黄芩素的工艺研究

目的应用黄芩茎叶葡萄糖醛酸水解酶(Scutellaria baicalensis stems and leaves glucuronic hydrolase,sbsl GUS)酶解灯盏花中野黄芩苷制备野黄芩素,经分离纯化获得高纯度的野黄芩素提取物。方法采用正交试验优选灯盏花提取工艺参数;以野黄芩苷酶解转化率为指标,对酶用量、酶解pH值、温度、时间、抗氧化剂等进行考察,优选野黄芩素制备工艺参数;采用乙醇提取、活性炭脱色和分步结晶精制纯化粗提物。结果灯盏花提取工艺为灯盏花切段,加10倍水煎煮提取2次,每次1 h。野黄芩素制备工艺为sbsl GUS提取液和灯盏花水煎液加入量(以生药计)比为1∶10,加0.5%偏重亚硫酸钠,酶解pH值约6.0,温度约45℃,时间20 h,得到含量大于60%的野黄芩素粗提物。经分步结晶对粗提物进行纯化,用80%乙醇回流提取,活性炭脱色,浓缩、析晶,得到含量大于85%的野黄芩素提取物。结论该工艺应用sbsl GUS酶解技术制备野黄芩素,生产简便可行,为野黄芩素的生产制备提供了新的途径。

栽培型和野生型灯盏花对Cd添加的生理响应

栽培驯化改变植物生物学和生理生态特性,但其如何影响药用植物对重金属污染的响应知之甚少。为研究Cd添加对栽培型和野生型灯盏花(Erigeron breviscapus(Vant.)Hand.-Mazz.)生物学性状、Cd吸收分配特征及生理响应的影响。采用盆栽试验,设置0、2.5、5和15 mg·kg^(-1)共四个Cd浓度梯度,采用隶属函数法综合评价两种类型灯盏花对Cd的耐性。结果表明:随Cd浓度增加,灯盏花植株Cd含量显著增加,但株高、SPAD值有降低趋势,而叶长宽、可溶性蛋白和MDA含量变化较小。在较高Cd添加浓度(15 mg·kg^(-1))下,两种类型灯盏花生物量显著高于不添加Cd处理,野生型SPAD值显著高于栽培型。同一Cd添加浓度的两种类型灯盏花SOD、POD、CAT活性和GSH、NPT、PCs含量无显著差异。两种类型灯盏花Cd的耐性指数大于1,平均Cd富集系数和转移系数大于0.5,但两种类型间无显著差异。栽培型和野生型灯盏花均具有较强的Cd富集能力和耐Cd特性,其中栽培型属中耐Cd型,野生型属高耐Cd型。栽培驯化后灯盏花耐Cd能力降低,转移和富集能力变化不明显。

民族药灯盏花药材及其混淆品的指纹图谱研究

通过UPLC指纹图谱,比较灯盏花及其混淆品不同药用部位的差异,结合相似度分析,为灯盏花药材鉴别和利用提供依据。采用Agilent ZORBAX Eclipse Plius C_(18)(?2.1 mm×100 mm, 1.8μm)色谱柱,以水(含0.1%甲酸)和乙腈(含0.1%甲酸)为流动相进行梯度洗脱,流速为0.2 mL/min,检测波长为300 nm。结果表明,建立的灯盏花指纹图谱共标定了19个共有峰;灯盏花茎、叶部与全草对照图谱无明显差异,但与花部对照图谱存在一定差异。建立的指纹图谱方法可对灯盏花及其混淆品加以鉴别,展苞飞蓬、多舌飞蓬与灯盏花的相似度较高,且多舌飞蓬、展苞飞蓬、狭苞紫菀、一年蓬和灯盏花花部的相似度较高。该研究有助于灯盏花药材的鉴别和利用,可为灯盏花药材的质量控制和开发提供参考。

灯盏细辛注射液通过调节JAK2/STAT3信号通路抗脑卒中大鼠神经损伤的研究

[目的]探讨灯盏细辛注射液(EBI)对脑卒中大鼠的神经保护作用及在该过程中对Janus激酶2(JAK2)/信号转导子和转录激活子3(STAT3)信号通路的调节机制。[方法]通过大脑中动脉闭塞(MCAO)建立缺血性脑卒中大鼠模型,然后将造模成功的大鼠随机分为模型组、尼莫地平组、EBI组、JAK2抑制剂组(AG490组)和EBI+JAK2激动剂组(EBI+CA1组),每组20只,另外选取20只大鼠只暴露颈动脉不进行结扎作为假手术组。利用神经功能缺损评分评估大鼠神经功能;2,3,5-三苯基氯化四氮唑(TTC)染色检测大鼠脑梗死面积;苏木精-伊红(HE)和尼氏(Nissl)染色检测大鼠脑组织的病理性形态;TUNEL染色检测大鼠脑组织中神经细胞凋亡情况;酶联免疫吸附测定(ELISA)检测大鼠脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的水平;蛋白免疫印迹法(Western blot)检测大鼠脑组织中Bax、B淋巴细胞瘤-2(Bcl-2)及JAK2、p-JAK2、STAT3、p-STAT3蛋白的表达。[结果]与假手术组相比,模型组大鼠脑梗死面积增大,神经元细胞凋亡率增加,神经缺损评分、MDA水平、Bax蛋白表达及p-JAK2/JAK2、p-STAT3/STAT3比值均升高(P<0.05),同时脑组织中神经元退化,神经元数量减少,SOD、GSH-Px活性及Bcl-2蛋白表达降低(P<0.05);与模型组相比,EBI组、尼莫地平组和AG490组大鼠脑梗死面积减小,神经元细胞凋亡率减少,神经缺损评分、MDA水平、Bax蛋白表达及p-JAK2/JAK2、p-STAT3/STAT3比值均降低(P<0.05),同时脑组织中神经元损伤减轻,神经元数量增加,SOD、GSH-Px活性及Bcl-2蛋白表达升高(P<0.05);进一步利用JAK2激动剂进行回补实验发现,EBI对大鼠神经损伤的保护作用被逆转(P<0.05)。[结论] EBI能够通过抑制JAK2/STAT3信号通路减轻脑卒中大鼠的神经损伤。

丹灯通脑软胶囊及其组方化学成分与分析方法研究进展

丹灯通脑软胶囊源于彝医验方,由丹参、灯盏细辛、川芎、粉葛4味药物组成,具有活血化瘀、祛风通络等功效,临床上多用于冠心病、脑中风、高血压、心绞痛等疾病的治疗。复方的组成药物中以黄酮类、醌类、有机酸类等化学成分为主,研究证实该药具有扩张周围毛细血管、促进心脑血管循环、增加冠状动脉血流量和缓解心肌缺血的功效,本文通过回顾近年来的相关文献,对丹灯通脑软胶囊的化学组成成分、质量控制和体内代谢研究方法进行总结,旨在为丹灯通脑软胶囊及相关组方的药效物质基础的研究提供参考依据。

灯盏花叶片数调控基因磷酸蛋白酶1的克隆与功能分析

【目的】探究灯盏花叶片数相关基因磷酸蛋白酶1(EbPP1)的功能,为揭示灯盏花叶片数目和开花时间分子机理奠定基础。【方法】从灯盏花叶片克隆EbPP1基因,将其构建至植物过表达载体RP101。用蘸花法侵染刚抽薹拟南芥植株进行异源表达,通过筛选获得若干株T_(1)、T_(2)、T_(3)代拟南芥阳性植株。最后统计T_(3)代阳性植株叶片数目和开花时间,通过实时荧光定量(RT-qPCR)检测EbPP1表达量,并测定拟南芥植株内源激素含量。【结果】成功克隆全长EbPP1基因,并构建该基因的重组过表达载体RP101-GFP-EbPP1。在拟南芥中实现稳定的遗传转化,于T_(3)代中获得24株阳性EbPP1转基因植株,并对T_(3)代阳性植株进行实时荧光定量分析,RT-qPCR结果显示转基因拟南芥中EbPP1基因过量表达,其表达量从野生型的1.034增加至136.330;转基因拟南芥在表型上呈现出叶片数增多、开花时间延迟的特征;通过LC-MS定量测定植株内源激素含量显示,T_(3)代转基因拟南芥中吲哚-3-甲酸(ICA)和赤霉素24(GA24)等多种激素的含量明显高于野生型,其中生长素前体物质L-色氨酸(TRP)尤为显著,达到野生型的3倍。【结论】本研究成功在拟南芥植株中异源表达EbPP1基因,与野生型相比,转基因拟南芥中叶片数增多,EbPP1基因表达量增高,内源激素含量升高。表明EbPP1可能通过调控植物激素水平,进而影响灯盏花叶片数目和开花时间。本研究为进一步解析灯盏花叶片数目发育相关机制和开花分子机理奠定基础,也为选育高品质灯盏花新品种提供一定遗传基础。

灯盏花胶囊对高脂联合STZ诱导2型糖尿病肾病小鼠的治疗作用研究

探讨灯盏花胶囊对糖尿病肾病(DN)模型小鼠的疗效.随机抽取雄性C57BL/6J小鼠8只为正常对照,剩余62只饲喂高糖高脂饲料12周后,采用腹腔注射链脲佐菌素复制DN模型;将40只成模小鼠随机分为模型对照、阳性苯那普利(10 mg/kg)以及灯盏花胶囊低(0.75 g/kg)、中(1.5 g/kg)、高剂量组(3.0 g/kg),每组8只;各组动物连续给药6周,正常和模型组灌胃等容积纯水.检测各组小鼠空腹血糖(FBG)、24 h尿蛋白及血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、白蛋白(ALB)、肌酐(Cre)、尿素氮(BUN)和胰岛素(FINS)水平;HE染色观察各组小鼠肾脏组织病理学变化.结果显示模型组小鼠给药期间血糖及尿蛋白水平缓慢升高,末期肾功能受到损害,血肌酐及尿素氮明显上升,病理检查发现肾小球明显肥大,系膜区扩张,基膜增厚;各组给药6周后,小鼠FBG、24 h尿蛋白及血清中BUN、CRE、TC、TG、LDL、FINS水平和肝肾指数较模型组明显降低(p<0.05/0.01),ALB明显提升(p<0.05/0.01),肾组织病变较模型组有所减轻.本研究表明灯盏花胶囊可以明显改善高糖高脂饮食联合链脲佐菌素诱发的2型糖尿病肾病小鼠的肾脏损害,一定程度保护肾功能,延缓肾功能减退.

灯盏细辛基于Psmb8/NF-κB轴抑制糖尿病肾病大鼠炎症反应

目的探讨灯盏细辛(erigeron breviscapus,EB)是否通过介导β型蛋白酶体亚基8(proteasome subunitβtype 8,Psmb8)及核因子-κB(nuclear factor-κB,NF-κB)轴抑制糖尿病肾病大鼠炎症反应。方法随机选10只SPF雄性SD大鼠作为正常组,剩余大鼠用高脂饮食联合链脲佐菌素(streptozotocin,STZ)构建糖尿病肾病(diabetic nephropathy,DN)大鼠模型。成模大鼠共41只,随机剔除1只,将剩余大鼠随机分为模型组、EB低剂量组、EB中剂量组和EB高剂量组,每组10只。其中EB低剂量、EB中剂量和EB高剂量组分别灌胃EB 20、40、80 mg·kg^(-1)·d^(-1),正常组和模型组大鼠给予等量生理盐水,持续给药8周。检测各组大鼠空腹血糖(fasting blood glucose,FBG)、肾脏指数(renal mass index,KI);用全自动生化分析仪检测24 h尿蛋白(urine protein,UPro)、血清肌酐(serum creatinine,Scr)和血尿素氮(blood urea nitrogen,BUN)水平;HE染色观察大鼠肾组织病理形态变化;用酶联免疫吸附试验(ELISA)检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)和白细胞介素-18(interleukin-18,IL-18)的含量;用实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测Psmb8和NF-κB p65 mRNA相对表达量;用蛋白印迹(Western blotting)法检测Psmb8、NF-κB p65及p-NF-κB p65蛋白的相对表达量。结果与正常组比较,模型组大鼠FBG、KI升高,体质量下降(P<0.05),肾小球形态见明显病理改变,间质水肿并伴有大量炎性细胞浸润,血清TNF-α、IL-1β及IL-18含量升高,肾组织中Psmb8和NF-κB p65 mRNA及Psmb8、NF-κB p65和p-NF-κB p65蛋白的相对表达量升高(P<0.05);与模型组比较,EB低、中、高剂量组大鼠FBG和KI下降,体质量升高(P<0.05),肾组织炎性病理情况见不同程度减轻,血清TNF-α、IL-1β及IL-18含量降低,肾组织中Psmb8和NF-κB p65 mRNA及Psmb8、NF-κB p65和pNF-κB p65蛋白的相对表达量降低(P<0.05);且EB对DN大鼠的炎症抑制作用呈剂量依赖性。结论EB可抑制DN大鼠炎症反应,其作用机制可能与抑制Psmb8/NF-κB轴激活有关。