The investigation for the relationship among serum leptin, erythrocyte membrane Ca^(2+)-ATPase activity and hypertensive disorder complicating pregnancy

Objective： To study the significance of Leptin and the activity of erythrocyte membrane Ca^2＋-ATPase（EMCA） in the development of hypertensive disorder complicating pregnancy. Methods： Radioimmunoassay was used to test the level of serum Leptin, and the activity of EMCA was determined chemically in 38 pregnant women with hypertensive disorder complicating pregnancy and 36 normotensive pregnant women. Results： The level of serum Leptin in hypertensive disorder complicating pregnancy（gestational hypertension： 13.76 ± 3.46 ng/ml; preeclampsia： 15.76 ± 5.47 ng/ml; eclampsia： 18.32 ± 6.38 ng/ml）was significantly higher than that in normotensive pregnant women （11.33 ± 2.93 ng/ml） ,respectively. The average EMCA activity of patients with hypertensive disorder complicating pregnancy （gestational hypertension： 1.65 ± 0.24 μmol·pi/mg.h ; preeclampsia： 1.37 ± 0.19 μ mol·pi/mg·h;eclampsia：1.12 ± 0.14 μmol·pi/mg·h） was significantly lower than that of normotensive pregnant women（1.83 ± 0.38 μ mol·pi/mg·h）,respectively. There was a negative correlation between the level of serum Leptin and the activity of RMCA in hypertensive disorder complicating pregnancy （r = -0.63）. Conclusion： Inhibition of EMCA activity of erythrocyte in hypertensive disorder complicating pregnancy may increase cytoplasmic free calcium, which contributes to the development of hypertensive disorder complicating pregnancy. The negative correlation between the level of serum Leptin and the activity of EMCA, also suggested that serum Leptin and the activity of EMCA may play a role in the development of hypertensive disorder complicating pregnancy.

Effect of Huoxuequyu Recipe on Erythrocyte Membrane Ca^(2+)-ATPase Activity in Pregnant Rats with Asymmetrical Intrauterine Growth Retardation Induced by Passive Smoking

The effect of Huoxuequyu recipe on erythrocyte membrane Ca2+-ATPase activity in pregnant rats with passive smoking induced asymmetrical intrauterine growth retardation(IUGR)was observed.The rats were divided into three groups:control group,model group and treated group.The fetal mean birth weight and erythrocyte membrane Ca2+-ATPase activity were found to be decreased in the model group as compared with the control and treated groups.There was a significant difference between the model group and the control group(P<0.01),while no significant difference existed between the treated group and the control group(P>0.05).Furthermore,the findings indicated that the erythrocyte membrane Ca2+-ATPase activity seem to be positively correlated with the birth weight(P<0.05).On the basis of our observation,it is believed that the Huoxuequyu decoction could increase erythrocyte membrane Ca2+-ATPase activity and promote blood circulation, thereby exerting a beneficial effect on fetal development.

The Relationship Between Senescence and Ca^(2+)-ATPase Activity of Microsomal Membrane and Lipid Peroxidation in Harvested Peach Fruit

Peach fruit easily soften and have a short storage time at normal temperature. In this study, peach fruit （Prunus persica sieb et Zucc cv. Yingqing） were picked and stored at 25 and 4℃ to investigate the senescence in correlation with Ca^2＋- ATPase activity of microsomal membrane and lipid peroxidation during ripening and senescence. In comparison with that stored at 25~C, the fruit stored at 4℃ exhibited a higher flesh firmness, lower respiration rate, and generated the late bigger peak value of Ca^2＋-ATPase activity as well as maintained the higher activity of the enzyme. Meanwhile, the lower levels of super oxygen radical （O2^-.） production and content of malondialdehyde （MDA）, a product of membrane lipid peroxidation were observed. Sodium orthovanadate （SO） and erythrosin B （EB）, as Ca^2＋-ATPase inhibitors, could stimulate the respiration rate. The results suggested that the slower senescence rate of peach fruit was closely related to the higher peak value and longer duration of Ca^2＋-ATPase activity in microsomal membrane, with the slighter membrane lipid peroxidation and lower O2^-. production rate.

Effect of Huoxuequyu Recipe on Erythrocyte Ultrastructure and Membrane ATPase Activity in Rats with Passive Smoking

The effect of 'Huoxuequyu' recipe on erythrocyte membrane Ca2+ATPase activity, Na+-K+-ATPase activity and erythrocyte ultrastructure in rats exposed to passive smoking were observed. The erythrocyte membrane Ca2+-ATPase activity and Na+-K+-ATPase activity were found to be decreased in the model group as compared with the control group (P<0.01, P<0.01), while no significant difference existed between the treated group and the control group (P>0.05). Our results also showed that there were more deformed red blood cells in model group than in control group under TCM and SEM (P<0.0001). No significant difference was found between control group and treated group (P>0.05). Furthermore,these findings indicated that 'Huoxuequyu' recipe , a prepared natural herbal medicine, may be involved in modulation of the activity of erythrocyte membrane enzymes,thereby protecting erythrocyte membrane, so as to improve the erythrocyte function.

甲状腺机能减退征患者红细胞膜Ca^(2+).Mg^(2+)-ATPase活性测定

目的了解红细胞膜钙-镁三磷酸腺苷酶(Ca2+.Mg2+-ATPase)活性在甲状腺功能减退征患者中的变化及该酶活性是否随甲状腺功能的恢复而逆转至正常,以确定该酶活性可否作为甲状腺激素在细胞水平作用的一个指标。方法低温、低渗溶解红细胞,差速离心法制备红细胞膜悬液。酶活性以含酶的红细胞膜悬液水解底物ATP释放出无机磷的量表示,无钙条件下无机磷的产生量为Mg2+-ATPase活性,有钙条件下无机磷的产生量为总ATPase活性,二者之差为Ca2+.Mg2+-ATPase活性。结果甲状腺功能减退患者红细胞Ca2+.Mg2+-ATPase活性明显低于正常组。但3月后,随着甲状腺功能恢复正常,酶活性也随之恢复正常。结论红细胞膜Ca2+.Mg2+-ATPase活性可作为甲状腺激素在细胞水平作用的一个指标,用于甲状腺功能异常的辅助诊断。

Effects of Aluminum on ATPase Activity and Lipid Composition of Plasma Membranes from Wheat Roots

The effects of aluminum on ATPase activity and lipid composition of the plasma membranes isolated from root tips of Al-tolerant (Altas 66) or Al-sensitive (Scout 66) cultivar of Triticum aestivum L. was assayed. The results showed that both cultivars had similar changes in H+-ATPase and Ca2+-ATPase activities after aluminum treatment. Exposure of both cultivars to 20 and 100 (mol/L aluminum for 5 d significantly decreased the activities of Ca2 + -ATPase of plasma membranes. The activities of H+-ATPase in plasma membrane increased under 20 μmol/L aluminum and decreased at 100 u.mol/L aluminum. With aluminum treatment, the PL content of plasma membrane decreased, but GL content increased. The ratio of PL to GL decreased more distinctly in Scout 66 than that in Altas 66. Treated with 20 and 100 μmol/L aluminum, linolenic acid content and the index of unsaturated fatty acids decreaced greatly in Scout 66, but the index of unsaturated fatty acids in Altas 66 increased slightly.

Effects of Xianzhen Tablet（仙贞片）on Na^+-K^+-ATPase and Ca^(2+)-Mg^(2+)-ATPase in Erythrocytic Membranes and Viscosity of Whole Blood in Non-Insulin-Dependent Diabetes Patients with Qi-Yin Deficiency, Kidney Deficiency and Blood Stasis

Objective: To assess the effects of Xianzhen tablet (XZT) on Na+-K+ -ATPase and Ca2+ Mg2+-ATPase on erythrocytic membranes, viscosity of whole blood, plasma glucose and clinical manifestations.Methods: Seventy-two cases of non-insulin-dependent diabetes mellitus (NIDDM) patients with deficiency of both Qi and Yin, deficiency of the Kidney and blood stasis were selected, and the effects of treatment on Na+ K + -ATPase, Ca2+-Mg2+ -ATPase, whole blood viscosity, blood sugar and clinical Symptoms were observed.Results: In XZT group (test group), activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase rose significantly(P< 0. 01, P< 0. 05) after treatment. Viscosity of whole blood and clinical manifestations also improved obviously. The total effective rate in lowering plasma glucose was 77. 8 % with fasting blood glucose (FBG) and 69.4 % with 2 hours postprandial plasma blood glucose (2°PBG). In the control group, viscosity of whole blood andclinical manifestations had no significant improvement. Its total effective rate in lowering plasma glucose was41. 7% with FBG and 38. 9% with 2°PBG. Conclusions: XZT played a certain role in increasing activities ofNa+ -K + -ATPase and Ca2+-Mg2+ -ATPase, decreasing viscosity of whole blood and plasma glucose and improving clinical manifestations. Therefore, XZT was experimentally manifested as an effective drug in treating NIDDM patients with Qi-Yin deficiency, renal deficiency and blood stasis.

妊娠期高血压疾病孕妇瘦素与红细胞膜Ca^(2+)-ATP酶活性的研究

目的:探讨瘦素、红细胞膜Ca2+-ATP酶活性在妊娠期高血压疾病发病中的意义及相互关系。方法:采用放射免疫法测定38例妊娠期高血压疾病患者,36例正常孕妇的血清瘦素。采用生化方法提取红细胞膜,测定红细胞膜Ca2+-ATP酶活性。结果:妊娠期高血压疾病患者血清瘦素水平明显高于对照组(15.95±5.10ng/m l vs 11.33±2.93ng/m l),红细胞膜Ca2+-ATP酶活性明显低于对照组[1.38±0.19μmol.pi/(mg.h)vs 1.83±0.38μmol.pi/(mg.h)](P<0.01)。妊娠期高血压疾病患者血清瘦素与红细胞膜Ca2+-ATP酶活性呈负相关(r=-0.63)。结论:血清瘦素水平与妊娠期高血压疾病的发生有关;妊娠期高血压疾病患者红细胞膜Ca2+-ATP酶活性降低引起细胞内游离Ca2+浓度升高,导致妊娠期高血压疾病发生;妊娠期高血压疾病患者血清瘦素水平与红细胞膜Ca2+-ATP酶活性呈负相关,两者共同参与妊娠期高血压疾病的发病。