Chemical composition and anti-MRSA activity of the essential oil from Chinese eaglewood

To analyze the constituents of essential oil from Chinese eaglewood [resinous wood of Aquilaria sinensis （Lour.） Gilg] and its anti-methicillin-resistant Staphylococcus aureus （MRSA） activity. The essential oil was extracted by water-steam distillation and analyzed by GC/MS method. The relative contents of the compounds were determined by normalization. The compounds were characterized by NIST05 and WILEY275L database matching and comparison of their MS spectra with those of literature data. Antibacterial activity of the oil was assayed by the filter paper disc agar diffusion method. The oil showed significant antibacterial activity against MRSA. Sixty-six chromatographic peaks were detected, among them thirty compounds comprising 59.80% of the total essential oil were characterized. Twenty-six compounds comprising 54.26% of the oil were identified as sesquiterpenes. β-Agarofuran （8.96%）, kusunol （7.82%）, （-）-jinkoh-eremol （5.04%）, agarospirol （4.53%）, baimuxifuranic acid （4.09%） were the major sesquiterpenes. Four nor-sesquiterpenes and some other sesquiterpenes, such as 10-epi-γ-eudesmol, α-agarofuran, epi-ligulyl oxide, etc. were detected in Chinese eaglewood oil for the first time. This is the first report about anti-MRSA activity of Chinese eaglewood oil from A. sinensis.

Composition of Essential Oils of Morus alba Leaves and Chamaecyparis pisifera Outer Heartwood

[Objective] The aim was to explore the composition of essential oils of Morus alba leaves and Chamaecyparis pisifera outer heartwood. [Method] The essential oils of M. alba leaves and C. pisifera outer heartwood were analyzed by gas chromatography-mass spectrometry,respectively. [Result] Alcohols [phytol （59.47%,extracted 3 h for M. alba leaves）,α-cadinol （38.77%,extracted 6 h for C. pisifera outer heartwood）] were their first principal volatile components. Phytol was usually used as pharmaceutical raw material and α-cadinol was a kind of active organic compound with antibacterial function. [Conclusion] The essential oils of M. alba leaves and C. pisifera outer heartwood with hydrodistillation have medicinal value.

Chemical Compositions and Antibacterial Activity of Essential Oil from Thymus Seedlings of Tissue Culture

[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. [Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry （GC/MS） and peak area normalization method; and the antimicrobial activity was tested by filter paper method. [Result] A total of 54 compositions were identified from these three samples, they had similar main compositions： thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. [Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.

Chemical composition, mechanism of antibacterial action and antioxidant activity of leaf essential oil of Forsythia koreana deciduous shrub

Objective: To identify the chemical constituents of leaf essential oil of Forsythia koreana(F. koreana) and evaluate its ef ects on bacterial strains. Methods: The essential oil of leaf of F. koreana was extracted by using hydrodistillation process and the volatile components investigated with the help of gas chromatography coupled with mass spectrometry. The antibacterial study was carried out with the help of agar disc dif usion method, MIC, MBC and viable count. The mode of action was determined with help of potassium ion l ux, cellular material release and scanning electron microscopy. The antioxidant activity was determined with the help of 2, 3-diphenyl-2-picrylhydrazyl method, nitric oxide scavenging activity and superoxide anion radical scavenging assay. Results: Total ten compounds were identii ed as trans-phytol(42.73%), cis-3-hexenol(12.95%), 毬-linalool(10.68%), trans-2-hexenal(8.86%), trans-2-hexenol(8.86%), myrcenol(3.86%), 4-vinylphenyl acetate(3.86%),(4Z)-4,6-heptadien-1-ol(3.18%), lemonol(2.73%) and benzeneacetaldehyde(2.27%) by gas chromatography coupled with mass spectrometry. The antibacterial study was demonstrated that leaf essential oil of F. koreana act against foodborne and other pathogenic bacteria. The mode of action revealed that this essential oil acted on the cytoplasmic membrane, resulting in loss of integrity and increased permeability. In addition, leaf essential oil of F. koreana was shown to be rich in linalool, which contributes to improved antioxidant activity. Conclusions: These results show that leaf essential oil of F. koreana has great potential as a natural food preservative, antibacterial and antioxidant agent.

Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) Hook.f.ex Benth (Rutaceae) against three mosquito species

Objective:To determine the mosquito larvicidal activity of leaf essential oil and their chemical constituents from Clausena anisata（C.anisata）（Willd.） Hook.f.ex Benth.against Culex quinquefasciatus,Aedes aegypti and Anopheles Stephensi.Methods:Essential oil was obtained by hydro-distillation and the chemical composition of the leaf essential oil was analyzed using gas chromatography—mass spectrometry.The mosquitoes were reared in the vector control laboratory and twenty lateⅢinstar larvae of three mosquito species were exposed to based on the wide range and narrow range tests,essential oil was tested at 50.100.150,200 and 250 ppm and each compound was tested at various concentration（5-75 ppm） and were assayed in the laboratory by using the protocol of WHO 2005:the 24 h LC50 values of the C.anisata leaf essential oil and their major compounds were determined following Probit analysis.Results:The oil contained were mainlyβ-pinene（32.8%）,sabinene（28.3%）,germacrene-D（12.7%）,estragole （6.4%） and linalool（5.9%）.The essential oil from the leaves of C.anisata exhibited significant larvicidal activity,with 24 h LC50 values of 140.96,130.19 and 119.59 ppm.respectively.The five pure constituents extracted from the C.anisata leaf essential oil were also tested individually against three mosquito larvae.The LC50 values ofβ-pinene,sabinene,germacrene-D,estragole and linalool appeared to be most effective against Anopheles stephensi(LC50-23.17,19.67,16.95. 11.01,35.17 ppm) followed by Aedes aegypti(LC50-27.69,21.20,18.76,12.70,38.64 ppm) and Culex quinquefasciatus(LC50-32.23,25.01.21.28,14.01,42.28).Conclusions:The essential oil of C. anisata contains five major compounds and has remarkable larvicidal properties,which may be considered as a potent source for the production of natural larvicides.

Composition and antimicrobial activity of Cistus munbyi essential oil: an endemic plant from Algeria

The chemical composition and antimicrobial potential of Cistus munbyi essential oil were studied for the first time. GC and GC–MS analysis revealed 48 compounds representing 96.98% of the oil. Terpinen-4-ol(23.75%), meta-Cymene(17.30%), and Sabinene(12.38%)were the major constituents. Antimicrobial activity was evaluated against nine pathogens using the disc diffusion and broth micro-dilution methods. Results show that C.munbyi essential oil possesses strong antimicrobial activity against all strains, regardless if Gram-positive or Gramnegative bacteria, or yeast, with MICs values not exceeding10(mg/ml). In addition to its efficacy, C. munbyi essential oil has an unusual antimicrobial potency which is attributed to its specific chemical composition. Thus, findings presented here suggest that endemic C. munbyi contains a very interesting essential oil that may be valuable in several areas.

Essential oils from Elaeoselinum asclepium: Chemical composition, antimicrobial and antioxidant properties

Objective: To evaluate the chemical composition of the essential oil isolated from Elaeoselinum asclepium(L.) Bertol.(E. asclepium), and test the efficiency of the essential oil as an antimicrobial and antioxidant agent.Methods: Essential oil was obtained from the aerial parts of E. asclepium by hydro distillation and analyzed by gas chromatography and gas chromatography coupled with mass spectrometry. We study for the first time the chemical composition of the essential oil of E. asclepium, followed by the in vitro antimicrobial activities, which were evaluated by agar diffusion method against six Gram-positive bacteria, five Gram-negative bacteria, and two fungi. In addition, The antioxidant activities were also investigated using assays of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and ferricreducing capacity.Results: The analyzed essential oil of the aerial parts of E. asclepium was rich in apinene(43.9%), other compounds detected in appreciable amounts were sabinene(27.9%) and b-pinene(16.0%). The essential oil yields 1.2%, the IC50 values of essential oil in 1,1-diphenyl-2-picrylhydrazyl assay in the reducing power assay were 48.26 mg/m L and at 1 mg/m L, respectively. The absorbance value of essential oil at 700 nm was0.956. The antimicrobial effect was higher on Candida albicans ATCC 1024 strain with the inhibition zone 14.5 mm than bacteria and molds.Conclusions: The essential oil of E. asclepium has antimicrobial and antioxidant activities. These species may be used as an important source of natural antimicrobial and antioxidant agents.

Essential oil content and composition of Indian sandalwood (Santalum album) in Sri Lanka

Santalum album （Indian Sandalwood） is found in the mountainous regions of the intermediate zone of Sri Lanka. Few studies have been conducted on sandalwood ecology in this region, and ours is the first recorded study of essential oil content and chemical composition of heartwood. We harvested two trees with State permission and took cross-sections for analysis. We demonstrated a difference in the heartwood formation and oil yield of the trees. The composition of the oil was found to be consistent between trees and along the trunk of the tree. Main aromatic compounds were santalols and other compounds are recorded in lesser quantities. Results of this study comply with the other published work on sandalwood elsewhere. This initial study on S. album in Sri Lanka provided promising results for the future of sandalwood agroforestry.

Comparative analysis of modern and ancient buried Phoebe zhennan wood:surface color,chemical components,infrared spectroscopy,and essential oil composition

We compared the chemical components and essential oils of ancient buried Zhennan（Phoebe zhennan）wood with those in samples from living trees.After removal of the carbon layer the recovered Zhennan exhibited a dark green color,which differed from the yellow color of the living samples.Low molecular weight components（including hot-water and toluene-alcohol extractives）,hemicellulose,and 1 % Na OH solubility in the recovered wood were greatly degraded.Degradation of cellulose was minor.Moreover,the ancient wood had somewhat more klason lignin than the modern wood.Fourier transform infrared（FTIR） analysis gave further evidence on the differences in chemical components.According to the GC–MS results,naphthalene derivatives were detected in the essential oils from both the modern and recovered wood.The delicate fragrance of the modern and recovered wood may be attributed to the aromatic constituents as identified by GC–MS.

Chemical Composition and Antimicrobial Activity of Essential Oil of Asarum caulescene

Both essential oils of fresh and air-dried Asarum caulescene （Maxim.） were analyzed by GC-MS in three different polarities. The resuits show that thirty-four components were identified in the oils, and main compounds of both oils were β-pinene （7. 63% vs. 20. 77%）, 2 carene （8.94% vs. 7.99%）, β-phellandrene （7.80% vs. 14.39%）, 1,8-cineole （8. 91% vs. 13. 29%） and germacrone （46. 64% vs. 6.64%）, however, the content of monoterpene hydrocarhons（30.29% vs. 52. 30%） and oxygenated sesquiterpenes（47.69% vs. 10.65%） of both oils was different. The antimicrobial activity of the oil was evaluated against 3 fungi and 11 hacteria including 7 clinical isolated strains. Our results showed that both oils presented a broad antimicrobial spectrum, and compared with the essential oil from fresh herb, the extracted oil from dried herb had hetter antimicrohial activity, its minimum inhibitory concentration（cmi） values of dried herh were 0. 16-2. 5 g/L and lowest minimum bactericidal concentration（cmb ） value was 0.31 g/L.

Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils

Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.

Chemical composition of Rosmarinus and Lavandula essential oils and their insecticidal effects on Orgyia trigotephras(Lepidoptera,Lymantriidae)

Objective:To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of 1 200 larvae were divided into three groups Ⅰ,Ⅱ,Ⅲ.Group Ⅰ was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis and ethanol were used as control group.Group Ⅱ was used as contact action and Group Ⅲ was used as fumigant action.Oecis and ethanol were used as control group.During the three experiments,the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined and,their insecticidal effects on the fourth larval slate of Orgyia trigotephras were assessed.The two simples presented an insecticidal activity,nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed.Conclusions:The relationship between the chemical composition and the biologieal activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can he considered as an alternative to the use of synthetic products.

Larvicidal efficacies and chemical composition of essential oils of Pinus sylvestris and Syzygium aromaticum against mosquitoes

Objective:To assess the chemical composition and mosquito larvicidal potentials of essential oils of locally sourced Pinus sylvestris(P.sylvestris)and Syzygium aromatkum(S.aromaticum)against Aedes aegypti(A.aegypti)and Culex quinquefasciatus(C.quinquefasciatus).Method:The chemical composition of the essential oils of both plants was determined using GCMS while the larvicidal bioassay was carried out using different concentrations of the oils against the larvae of A.aegypti and C.quinquefasciatus in accordance with the standard protocol.Results:The results as determined by GC-MS showed that oil of S.aromaticum has eugenol(80.5%)as its principal constituent while P.sylvestris has 3-Cyclohexene-1-methanol,.alpha.,.alpha.4-trimethyl(27.1%)as its dominant constituent.Both oils achieved over 85%larval mortality within24 h.The larvae of A.aegypti were more susceptible to the oils[LC_(50)(S.aromaticum)=92.56 mg/L,LC_(50)(P.sylvestris)=100.39 mg/L]than C.quinquefasciatus[LC_(50)(S.aromaticum)=124.42 mg/L;LC_(50)(P.sylvestris)=128.00 mg/L].S.aromaticum oil was more toxic to the mosquito larvae than oil of P.sylvatris but the difference in lethal concentrations was insignificant(P>0.05).Condusioii:The results justify the larvicidal potentials of both essential oils and the need to incorporate them in vector management and control.

Chemical compositions and biological activities of essential oils obtained from some Apiaceous and Lamiaceous plants collected in Thailand

Objective: To determine the chemical composition, as well as the antioxidant, antityrosinase and antibacterial activities of essential oils obtained from some Apiaceous and Lamiaceous plants collected in Thailand. Methods: The essential oils of the specified spices and aromatic herbs were obtained by hydro-distillation, and their chemical constituents were analyzed by gas chromatography/mass spectrometry. Antioxidant assays were based on the scavenging effects of 2,2-Diphenyl-1-picrylhydrazyl(DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS) free radicals as well as the lipid oxidation inhibition of β-carotene bleaching by linoleic acid. Tyrosinase enzyme inhibition was evaluated by the dopachrome method. Broth microdilution technique was performed for the purposes of studying microbial growth inhibition against the isolated bacterial strains. Results: The essential oils of Elsholtzia stachyodes, Coleus amboinicus(I) and Trachyspermum ammi presented a high degree of potency in DPPH, ABTS and β-carotene bleaching assays. The Trachyspermum ammi oil, which mainly contained thymol(49.04%) and p-cymene(22.06%), proved to be the most effective in terms of antibacterial activity. The major compositions of Coleus amboinicus(I) were carvacrol(51.57%), γ-terpinene(18.04%) and p-cymene(7.81%); while thymol(43.76%) and γ-terpinene(24.61%) were identified as the major components of Elsholtzia stachyodes oil, with p-cymene(6.73%) being identified as a minor constituent. Moreover, Cuminum cyminum oil containing cuminaldehyde(49.07%) and Elsholtzia communis oil composed with geranial(44.74%) and neral(35.27%) as the major components displayed a specific ability for the inhibition of the mushroom tyrosinase enzyme. Conclusions: The results indicated that these bioactive essential oils obtained from indigenous herbs are of significant interest as alternative raw materials in food, cosmetic and medicinal products.

Chemical composition of essential oils and in vitro antioxidant activity of fresh and dry leaves crude extracts of medicinal plant of Lactuca Sativa L.native to sultanate of Oman

Objective:To isolate and analyse the chemical composition in the essential oils and free radical scavenging activity of different crude extracts from the fresh and dry leaves of vegetable plants of Lactuca sativa L.(L.sativa).Methods:The essential oils and volatile chemical constituents were isolated from the fresh and dry leaves of L.sativa(lettuce)grown in Sultanate of Oman by hydro distillation method.The antioxidant activity of the crude extracts was carried out by well established free radical scavenging activity(DPPH)method.Results:About 20 chemical compounds of different concentration representing 83.07%and 79.88%respectively were isolated and identified by gas chromatography-mass spectroscopy in the essential oils isolated from the fresh and dry leaves asα-pinene(5.11%and 4.05%).γ-cymene(2.07%and 1.92%),thymol(11.55%and 10.73%),durenol(52.00%and 49.79%),α-terpinene(1.66%and 1.34%),thymol acetate(0.99%and 0.67%),caryophyllene(2.11%and 1.98%),spathulenol(3.09%and 2.98%).camphene(4.11%and3.65%),limonene(1.28%and 1.11%)representing these major chemical compounds.However,some other minor chemical constituents were also isolated and identified from the essential oil of lettuce including p-pinene,α-terpinolene,linalool,4-terpineol,α-terpineol,o-methylthymol,L-alloaromadendrene and viridiflorene.Conclusions:The chemical consliluents in the essential oils from the locally grown lettuce were identified in the following classes or groups of chemical compounds such as monoterpenes,sesquiterpenes volatile organic compounds and their oxygenated hydrocarbons.Therefore,the essential oils and the crude extracts from Omani vegetable species of lettuce are active candidates which would be used as antioxidant,antifungal or antimicrobial agents in new drugs preparation for therapy of infectious diseases.

Chemical Composition and Biological Activities of Essential Oil from the Rhizomes of Iris bulleyana

Iris bulleyana has long been used as a remedy for detoxication and detumescence. Hydrodistillation was used to extract the essential oil from its rhizomes, and 0.23% oil yield was obtained. Using gas chromatography-mass spectrometry （GC- MS） analysis, 31 chemicals including aristolone, cuparene, β-gurjunene, δ-amorphene, α-muurolene, α-cadinol, camphor, γ-elemene, and τ-cadinol were identified. The essential oil exhibited antibacterial activity against Acetobacter calcoacetica, Bacillus subtillis, Clostridium sporogenes, Clostridium perfringens, Escherichia coli, Salmonella typhii, Staphylococcus aureus, and Yersinia enterocolitica. Its antifungal and antioxidant activities were also tested.

Chemical composition and larvicidal effects of essential oil of Blumea martiniana against Anopheles anthropophagus

Objective:To determine chemical composition of the hydrodistilled essential oil of Blumea martiniana,and to evaluate its larvicidal activity against Anopheles anthropophagus,the natural vector of malaria.Methods:Chemical composition of essential oils extracted by steam distillation was investigated by gas chromatography and mass spectroscopy（GC-MS）.Larvicidal activity of essential oil and its four main compounds was carried out by WHO method.Twenty larvae of early fourth-instar stage were used in the larvicidal assay and five replicates were maintained for each concentration.The 24 h LC50 and LC90 values were determined following probit analysis.Results:Totally 68 compounds corresponding to 98.55%of the total oil were identified and the major constituents of essential oil were:linalool（10.36%）,germacrene D（9.09%）, borneol（6.24%）,γ-terpinene（5.38%）.The oil and linalool,germacrene D,bomeol,γ-terpinene exerted significant larvicidal activity with LC50 values of 46.86,35.87,44.61,35.89,and 29.21 mg/L, respectively.It also showed a dose dependent effect on mortality.Conclusions:The essential oil of Blumea martiniana and its four major compounds may have potential for use in control of Anopheles anthropophagus larvae and could be useful in search of newer,safer and more effective natural compounds as larvicides.

Chemical composition and antimicrobial activity of essential oil isolated from the cultured mycelia of Ganoderma japonicum

Objective：To explore a new natural antibiotic. Methods：The chemical composition of the essential oil from Ganodermajaponicum （G. japonicum）mycelia was analyzed by gas chromatography-mass spectrometry（GC-MS）, The antimicrobial activity of the oil was evaluated against eighteen microorganisms, including bacteria, mildew and yeast by using a disc diffusion method. Furthermore, the minimum inhibitory concentrations（MIC） and the minimum bactericidal concentrations（MBC） of the essential oil against twelve clinical pathogens were determined. Results：The main components of the oil were nerolidol, decadienal, linalool and benzyl alcohol. The antimicrobial results indicated that the oil inhibited all the tested bacterium, especially Methicillin-resistant Staphylococcus aureus （MRSA） in which the antibacterial activity exhibited a MBC of 1.03 mg/ml. Conclusion： The essential oil of G. japonicum mycelium has significant inhibitory activity. It is a potential medicinal resource that can be used as a natural antibiotic.

Chemical Composition of Bergamot （Citrus Bergamia Risso） Essential Oil Obtained by Hydrodistillation

Peel essential oil of bergamot （Citrus bergamia Risso） growing in Tunisia was separated by hydrodistillation and obtained in a yield of 9.7%. The oil composition was investigated using GC and GC-MS with two columns HP-I and HP-Innowax. Fifteen compounds accounting for 98.52% of the oil were identified. The oil was characterized by bigb content oflimonene （59.21%）, linalool （9.51%） and linalyl acetate （16.83%）.

Chemical Composition, Antibacterial and Antioxidant Activities of Thyme Essential Oil (<i>Thymus vulgaris</i>)

Herbal medicine from natural resources plays an important role as antibacterial and antioxidant agents. The present investigation was designed to evaluate the antibacterial and antioxidant properties of thyme (Thymus vulgaris L.) essential oil (TEO) and/or chitosan (CH) in vitro. Results indicated that TEO exhibited high radical scavenging activity (RSA) toward DPPH, ABTS, linoleic acid deterioration and iron chelation activity. TEO exhibited high amount of total phenolic compounds (TPC) related to its terpenes. The TPC of TEO was 177.3 mg GAE g-1 demonstrated 149.8 μmol of TE g-1 DPPH-RSA and 192.4 μmol of TE g-1 ABTS-RSA. The antioxidant capacity of TEO exhibited 68.9% reduction when evaluated by β-carotene bleaching assay. The reducing power activity related to iron chelation was 142.8 μmol of AAE g-1. The TEO exhibited a high content of Thymol (41.04%) as major compound over 14 identified components by GC-MS analysis followed by 1,8-Cineole (14.26%), γ-Terpinene (12.06%), p-Cymene (10.50%) and α-Terpinene (9.22%). TEO exhibited antimicrobial activity in vitro and MIC noticed that TEO was efficiently affected pathogens in vitro. Indeed, CH exhibited negligible or very low antimicrobial activity. In conclusion, both investigated TEO and TEO-CHmix have strong antibacterial activity against many pathogenic bacteria and need exploitation as an alternative source of natural antibacterial and antioxidant agents for potential applications.