Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult hom...Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type （WT） cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging, ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass： 23 previously reported genes （Calcr, Cebpb, Crtap, Dcstamp, Dkkl, Duoxa2, Enppl, Fgf23, Kissl/Kisslr, Kl （Klotho）, Lrp5, Mstn, Neol, Npr2, Ostml, Postn, Sfrp4, S1c30a5, Sic39a13, Sost, Sumf1, Src, Wnt10b）, five novel genes extensively characterized （Cldn18, Fam20c, Lrrkl, Sgpll, Wnt16）, five novel genes with preliminary characterization （Agpat2, RassfS, Slc10a7, Stc26a7, Slc30a10） and three novel undisclosed genes coding for potential osteoporosis drug targets.展开更多
The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute pha... The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).……展开更多
BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at diffe...BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at different sites. OBJECTIVE： To compare the features of animal models of complex partial epilepsy established through unilateral, bilateral and alternate-side kindling at hippocampus and successful rate of modeling among these 3 different ways. DESIGN： A randomized and controlled animal experiment SETTING： Department of Neurology, Qilu Hospital, Shandong University MATERIALS： Totally 60 healthy adult Wistar rats, weighing 200 to 300 g, of either gender, were used in this experiment. BL-410 biological functional experimental system （Taimeng Science and Technology Co. Ltd, Chengdu） and SE-7102 type electronic stimulator （Guangdian Company, Japan） were used in the experiment. METHODS： This experiment was carried out in the Experimental Animal Center of Shandong University from April to June 2004. After rats were anesthetized, electrode was implanted into the hippocampus. From the first day of measurement of afterdischarge threshold value, rats were given two-square-wave suprathreshold stimulation once per day with 400 μA intensity, 1ms wave length, 60 Hz frequency for 1 s duration. Left hippocampus was stimulated in unilateral kindling group, bilateral hippocampi were stimulated in bilateral kindling group, and left and right hippocampi were stimulated alternately every day in the alternate-side kindling group. Seizure intensity was scored： grade 0： normal, 1： wet dog-like shivering, facial spasm, such as, winking, touching the beard, rhythmic chewing and so on; 2： rhythmic nodding; 3： forelimb spasm;4： standing accompanied by bilateral forelimb spasm;5： tumbling, losing balance, four limbs spasm. Modeling was successful when seizure intensity reached grade 5. t test was used for the comparison of mean value between two samples. MAIN OUTCOME MEASURES： Comparison of the successful rate of modeling, the times of stimulation to reach intensity of grade 5, the lasting time of seizure of grade 3 of rats in each group. RESULTS： Four rats of alternate-side kindling group dropped out due to infection-induced electrode loss, and 56 rats were involved in the result analysis. The successful rate of unilateral kindling group, bilateral kin- dling group and alternate-side kindling group was 55%（11/20）,100%（16/16）and 100%（20/20）, respective- ly. The stimuli to reach the grade 5 spasm were significantly more in the bilateral kindling group than in the unilateral kindling group [（30.63±3.48）, （19.36±3.47）times, t=8.268, P 〈 0.01], and those were significantly fewer in the alternate-side kindling group than in the unilateral kindling group [（ 10.85±1.98）times, t=-8.744, P 〈 0.01]. The duration of grade 3 spasm was significantly longer in the bilateral kindling group than in the unilateral kindling group [（9.75±2.59）, （3.21 ±1.58）days,t=-8.183,P 〈 0.01], Among 20 successful rats of al- ternate-side kindling group, grade 5 spasm was found in the left hippocampi of 11 rats, but grade 3 spasm in their right hippocampi; Grade 5 spasm was found in the right hippocampi of the other 9 rats, grade 4 spasm in the left hippocampus of 1 rat and grade 3 of 8 rats. CONCLUSION ： The speed of establishing epilepsy seizure model by alternate-side kindling is faster than that by unilateral kindling, while that by bilateral kindling is slower than that by unilateral kindling. The successful rate is very high to establish complex partial epilepsy with alternate-side or bilateral kindling. Epilepsy seizure established by alternate-side kindling has antagonistic effect of kindling and the seizure duration of grade 3 spasm is prolonged.展开更多
Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department o...Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department of Environmental Pollution and Process Control, and Department of Central Asian Cooperation and Coordinated Development, respectively. Now, there are a total of eight research departments in XJEG, CAS.展开更多
The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power gene...The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power generating capability. The State-owned corporation, with 2.5 billion yuan ($301 million) worth of operating capital raised by the government, will be responsible for investment. construction and management of the power transmission and substation projects involved in the construction of the Three Gorges Project.展开更多
China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevan... China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevant professional standards on high performance computers to break through the monopoly in the field by foreign manufacturers and vendors.……展开更多
The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Repre...The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Representatives of China,Russia,Italy,Thailand and Pakistan signed the international standards alliance initiative,in an effort to create a new situation for the application of proton,superconductor and nuclear energy based on collaboration,sharing and win-win.展开更多
Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. App...Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. Apporved by the State Council, the Certification and Accreditation Administration of China (CNCA), which is in charge of supervising, administrating and coordinating certification and accreditation departments of the whole nation, has done a lot of work to achieve this goal.展开更多
The Chinese audiovisual market is to impose a ban on audiovisual product dealers whose licenses have been revoked for violatingthe law. This ban will prohibit them from dealing in audiovisual products for ten year...The Chinese audiovisual market is to impose a ban on audiovisual product dealers whose licenses have been revoked for violatingthe law. This ban will prohibit them from dealing in audiovisual products for ten years. Their names are to be included on a blacklist made known to the public.展开更多
A novel laser facility, named SG-Ⅱ (Shenguang, or Divine Light), has recently passed the appraisal of a panel of experts from the Chinese Academy of Sciences (CAS), China Academy of Engineering Physics, and State... A novel laser facility, named SG-Ⅱ (Shenguang, or Divine Light), has recently passed the appraisal of a panel of experts from the Chinese Academy of Sciences (CAS), China Academy of Engineering Physics, and State High-tech Development Program (National 863 Program). ……展开更多
The Tianlian 1-03 satellite, the third geosynchronous data relay satellite of China, was successfully launched into space on a LM-3C launch vehicle from the Xichang Satellite Launch Center at 23:43 Beijing time on Jul...The Tianlian 1-03 satellite, the third geosynchronous data relay satellite of China, was successfully launched into space on a LM-3C launch vehicle from the Xichang Satellite Launch Center at 23:43 Beijing time on July 25. Twenty-six minutes after the liftoff, the satellite展开更多
文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type （WT） cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging, ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass： 23 previously reported genes （Calcr, Cebpb, Crtap, Dcstamp, Dkkl, Duoxa2, Enppl, Fgf23, Kissl/Kisslr, Kl （Klotho）, Lrp5, Mstn, Neol, Npr2, Ostml, Postn, Sfrp4, S1c30a5, Sic39a13, Sost, Sumf1, Src, Wnt10b）, five novel genes extensively characterized （Cldn18, Fam20c, Lrrkl, Sgpll, Wnt16）, five novel genes with preliminary characterization （Agpat2, RassfS, Slc10a7, Stc26a7, Slc30a10） and three novel undisclosed genes coding for potential osteoporosis drug targets.
文摘 The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).……
文摘BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at different sites. OBJECTIVE： To compare the features of animal models of complex partial epilepsy established through unilateral, bilateral and alternate-side kindling at hippocampus and successful rate of modeling among these 3 different ways. DESIGN： A randomized and controlled animal experiment SETTING： Department of Neurology, Qilu Hospital, Shandong University MATERIALS： Totally 60 healthy adult Wistar rats, weighing 200 to 300 g, of either gender, were used in this experiment. BL-410 biological functional experimental system （Taimeng Science and Technology Co. Ltd, Chengdu） and SE-7102 type electronic stimulator （Guangdian Company, Japan） were used in the experiment. METHODS： This experiment was carried out in the Experimental Animal Center of Shandong University from April to June 2004. After rats were anesthetized, electrode was implanted into the hippocampus. From the first day of measurement of afterdischarge threshold value, rats were given two-square-wave suprathreshold stimulation once per day with 400 μA intensity, 1ms wave length, 60 Hz frequency for 1 s duration. Left hippocampus was stimulated in unilateral kindling group, bilateral hippocampi were stimulated in bilateral kindling group, and left and right hippocampi were stimulated alternately every day in the alternate-side kindling group. Seizure intensity was scored： grade 0： normal, 1： wet dog-like shivering, facial spasm, such as, winking, touching the beard, rhythmic chewing and so on; 2： rhythmic nodding; 3： forelimb spasm;4： standing accompanied by bilateral forelimb spasm;5： tumbling, losing balance, four limbs spasm. Modeling was successful when seizure intensity reached grade 5. t test was used for the comparison of mean value between two samples. MAIN OUTCOME MEASURES： Comparison of the successful rate of modeling, the times of stimulation to reach intensity of grade 5, the lasting time of seizure of grade 3 of rats in each group. RESULTS： Four rats of alternate-side kindling group dropped out due to infection-induced electrode loss, and 56 rats were involved in the result analysis. The successful rate of unilateral kindling group, bilateral kin- dling group and alternate-side kindling group was 55%（11/20）,100%（16/16）and 100%（20/20）, respective- ly. The stimuli to reach the grade 5 spasm were significantly more in the bilateral kindling group than in the unilateral kindling group [（30.63±3.48）, （19.36±3.47）times, t=8.268, P 〈 0.01], and those were significantly fewer in the alternate-side kindling group than in the unilateral kindling group [（ 10.85±1.98）times, t=-8.744, P 〈 0.01]. The duration of grade 3 spasm was significantly longer in the bilateral kindling group than in the unilateral kindling group [（9.75±2.59）, （3.21 ±1.58）days,t=-8.183,P 〈 0.01], Among 20 successful rats of al- ternate-side kindling group, grade 5 spasm was found in the left hippocampi of 11 rats, but grade 3 spasm in their right hippocampi; Grade 5 spasm was found in the right hippocampi of the other 9 rats, grade 4 spasm in the left hippocampus of 1 rat and grade 3 of 8 rats. CONCLUSION ： The speed of establishing epilepsy seizure model by alternate-side kindling is faster than that by unilateral kindling, while that by bilateral kindling is slower than that by unilateral kindling. The successful rate is very high to establish complex partial epilepsy with alternate-side or bilateral kindling. Epilepsy seizure established by alternate-side kindling has antagonistic effect of kindling and the seizure duration of grade 3 spasm is prolonged.
文摘Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department of Environmental Pollution and Process Control, and Department of Central Asian Cooperation and Coordinated Development, respectively. Now, there are a total of eight research departments in XJEG, CAS.
文摘The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power generating capability. The State-owned corporation, with 2.5 billion yuan ($301 million) worth of operating capital raised by the government, will be responsible for investment. construction and management of the power transmission and substation projects involved in the construction of the Three Gorges Project.
文摘 China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevant professional standards on high performance computers to break through the monopoly in the field by foreign manufacturers and vendors.……
文摘The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Representatives of China,Russia,Italy,Thailand and Pakistan signed the international standards alliance initiative,in an effort to create a new situation for the application of proton,superconductor and nuclear energy based on collaboration,sharing and win-win.
文摘Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. Apporved by the State Council, the Certification and Accreditation Administration of China (CNCA), which is in charge of supervising, administrating and coordinating certification and accreditation departments of the whole nation, has done a lot of work to achieve this goal.
文摘The Chinese audiovisual market is to impose a ban on audiovisual product dealers whose licenses have been revoked for violatingthe law. This ban will prohibit them from dealing in audiovisual products for ten years. Their names are to be included on a blacklist made known to the public.
文摘 A novel laser facility, named SG-Ⅱ (Shenguang, or Divine Light), has recently passed the appraisal of a panel of experts from the Chinese Academy of Sciences (CAS), China Academy of Engineering Physics, and State High-tech Development Program (National 863 Program). ……
文摘The Tianlian 1-03 satellite, the third geosynchronous data relay satellite of China, was successfully launched into space on a LM-3C launch vehicle from the Xichang Satellite Launch Center at 23:43 Beijing time on July 25. Twenty-six minutes after the liftoff, the satellite
