PERINATAL FETAL WELL-BEING MONITORING: A COMPARISON BETWEEN RANDOMIZED URINARY ESTRIOL/CREATININE AND SERUM FREE ESTRIOL DETERMINATION

Randomized urinary and blood samples were obtained from three hundred and ninety-two cases of late pregnancies simultaneously for comparison of the validity in perinatal fetal monitoring. The results of randomized urinary estriol/creatinine （RUE3/C） and serum free estriol （SFE3） tests revealed that Ⅰ. The coefficient of correlation between RUE3/C and SFE3 level were close in either normal or high-risk pregnancies （y=0.1477X+14.2368, r=0.4586, n=170; y=0.1724X+8.6698, r=0.5868, n=222, respectively, P【0.001）; 2.92%cases of the false positive rate of RUE3/C were corrected by SFE3 test. In cases of pregnancy-induced hypertension （PIH） the false positive rate of RUE3/C were highest in comparison with those in cuses of other high-risk pregnancies （53.6%）; 3. The correct rate of SFE3 test in prenatal predication of fetal well-being was significantly higher （95.6%） than that of RUE3/C （87.3%） （X2=17.77, P【0.001）. This paper suggests that RUE3/C can be used as a first line test for perinatal monitoring. When RUE3/C value is lower than normal, SFE3 test should be added to ensure the reliability of perinatal diagnosis, especially in PIH cases. Obviously, to use these two tests for perinatal fetal monitoring simultaneously is better than to use any one test alone.

Voltammetric sensor based on cobalt-poly(methionine)-modified glassy carbon electrode for determination of estriol hormone in pharmaceuticals and urine

A voltammetric sensor based on the electropolymerization of cobalt-poly(methionine)(Co-poly(Met)) on a glassy carbon electrode (GCE) was developed and applied for the determination of estriol by differential pulse voltammetry (DPV) for the first time. The electrochemical properties of the Co-poly(Met)/GCE were analysed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) were used to characterize the polymers on the GCE surface. The deposition of the Co-poly(Met) film on the GCE surface enhanced the sensor electronic transfer. CV studies revealed that estriol exhibits an irreversible oxidation peak at t0.58 V for the Co-poly(Met)/GCE (vs. Ag/AgCl reference electrode) in 0.10 mol/L Britton-Robinson buffer solution (pH=7.0). Different voltammetric scan rates (10-200 mV/s) suggested that the estriol oxidation on the Co-poly(Met)/GCE surface is controlled by adsorption and diffusion processes. Based on the optimized DPV conditions, the linear responses for estriol quantification were from 0.596 μmol/L to 4.76 μmol/L (R2 =0.996) and from 5.66 μmol/L to 9.90 μmol/L (R2 =0.994) with a limit of detection (LOD) of 0.0340 μmol/L and a limit of quantification (LOQ) of 0.113 μmol/L. The DPV-Co-poly(Met)/GCE method provided good intra-day and inter-day repeatability with RSD values lower than 5%. Also, no interference of real sample matrices was observed on the estriol voltammetric response, making the DPV-Copoly( Met)/GCE highly selective for estriol. The accuracy test showed that the estriol recovery was in the ranges 96.7%-103% and 98.7%-102% for pharmaceutical tablets and human urine, respectively. The estriol quantification in pharmaceutical tablets performed by the Co-poly(Met)/GCE-assisted DPV method was comparable to the official analytical protocols.

A NEW TEST IN ANTENATAL MONITORING-THE EVALUATION OF SALIVARY UNCONJUGATED ESTRIOL MEASUREMENT

A new unconjugated saliva estriol （SE3） determination by radioimmunoassay（3H-E3） was used to monitor 497 fetuses of late pregnancies, including 280 high-risk and217 normal pregnant women. The normal pregnant SE3 values had been established by themeasurement of 1378 cases from 20 to 41 weeks of gestation. The results of SE3 assaysrevealed that 1. In prenatal prediction of fetal well-being, the total false negative andfalse positive rates were 2.0% and 0.6%, respectively, the correct rate which was similarto that of serum free E3 （SFE3） values was 97.4%, and was significantly higher thanthat of overnight 12-hour urine E3/C analyses as formerly reported. 2. There were 8 fetaldeathe in 11 cases with low SE3 levels. No perinatal death occurred in the 486 cases withnormal SE3 values, except one fetus who died of nuchal cord strangulation. It is highlyimportant that the saliva specimen be correctly collected and the technique ofmeasurement of SE3 be carefully carried out. Our observations suggest that the clinicaluse of SE3 assays are scientific, reliable, and it is more practicable than that of SFE3 as-says. The determination of SFE3 can be replaced by SE3 test for assessing fetal-placentalwell-being.

Quality of Life before and after the Use of Vaginal Estriol in Postmenopausal Women with Lower Urinary Tract Symptoms

Background: Nowadays, a substantial number of women spend more than one-third of their lives in the postmenopausal period. It is characterized by a marked decrease in the production of estrogen, which leads to urogenital atrophy. The symptoms stemming from vulvovaginal atrophy and the lower urinary tract are currently referred to as the Genitourinary Syndrome of Menopause (GSM), which can have a negative impact on the quality of life (QoL). Estrogen replacement is the ideal treatment for GSM, and vaginal administration is the most recommended. Objective: To assess the impact of the use of topical vaginal estriol on the quality of life (QoL) of postmenopausal women with lower urinary tract symptoms (LUTS). Methods: This is an interventional, prospective study, performed in 49 women at the Antonio Pedro Hospital, at the Universidade Federal Fluminense, in Niterói, Brazil, from August 2014 to April 2015. It included postmenopausal women with lower urinary tract symptoms who were not using any estrogen hormone therapy, and it excluded those with contraindications for the use of estriol. A specific questionnaire on QoL and urinary tract symptoms, the King’s Health Questionnaire (KHQ), which identifies LUTS and assesses to what extent those symptoms interfere with QoL, has been used as a research tool. Results: The average age was 62.24 years. Urinary urgency and urge incontinence were reported by 91.8% of women. The average scores of the domains of the KHQ decreased in the domains General Health Perception (before: 46.42 ± 21.65;after: 40.81 ± 22.64), Incontinence Impact (before: 74.82 ± 27.66;after: 41.49 ± 30.83), Role Limitations (before: 43.20 ± 32.80;after: 21.09 ± 24.71), Physical Limitations (before: 38.09 ± 32.09;after: 14.62 ± 24.20), Social Limitations (before: 30.38 ± 28.75;after: 12.62 ± 19.85), Emotions (before: 43.31 ± 32.96;after: 20.18 ± 26.41), Sleep/Energy (before: 41.48 ± 37.74;after: 15.98 ± 23.31) and Severity Measures (before: 48.02 ± 24.68;after: 22.31 ± 20.25). All the differences were statistically significant (p-value ≤ 0.05). Conclusion: In the group of postmenopausal women with lower urinary tract symptoms included in the study, the use of topical vaginal estriol led to a decrease in the frequency of each symptom and the average scores in all domains of the KHQ, suggesting a positive effect on women’s QoL.

Modeling the aqueous reaction kinetics of estriol with ferrate

In this study the aqueous oxidation kinetics of estriol(E3)by potassium ferrate(K_(2)FeO_(4)),a chemical for its strong oxidizing power and for producing a coagulant from its reduced state(i.e.Fe(III)),was evaluated in the range of pH 8–12 with different molar ratios of the reactants.As the degree of Fe(VI)protonation varies with the solution pH,it was found that afirst order model was not suitable to describe the oxidation reaction.This paper describes a theoretical representation that closely models the reaction kinetics of E3 and ferrate.From this modeling,the reaction rates of HFeO_(4)^(-) and FeO_(4)^(2-) with E3 have been determined.The results show that the reactivity of HFeO–with dissociated and undissociated E3 is greater than that4 of FeO_(4)^(2-),and that E3 is more reactive in its dissociated state.

An UPLC-MS/MS method to monitor Estriol injection and comparison of pharmacokinetic characteristics after irradiation

Objective:To develop an UPLC-MS/MS method for the accurate quantification of Estriol(E3),a new radioprotective agent,and observe the variation in pharmacokinetic characteristics of E3 after irradiation.As a hormone drug,the gender differences of E3 metabolism were also concerned here.Methods:Various chromatographic and mass spectrometric conditions of E3 were optimized.The specificity,linearity,precision and accuracy of UPLC-MS/MS method were validated.Twenty SD rats,half male and half female,were administered with intramuscular(im)injection of 2.7 mg/kg E3,and then divided randomly into two groups,sham-irradiated group(Con)and irradiated group(IR).IR group was irradiated to 7 Gy ofγ-rays.Blood samples were collected at different times post-irradiation and E3 concentrations were detected.The changes of concentration-time variation and pharmacokinetic parameters for E3 after irradiation were investigated,together with metabolic differences between male and female rats.Results:The range of the calibration curve of UPLC-MS/MS for E3 was 1.00–200.0 ng/mL.Con group reached maximum concentration(Cmax)(77.57±18.71)ng/mL at(0.68±0.29)h(Tmax)after im injection.The drug concentration-time profiles and pharmacokinetic parameters of E3 were consistent before and after irradiation.The areas under time curve(AUC0-t)of E3 were(353±74)h⋅ng/mL for Con group,and(299±74)h⋅ng/mL for IR group(P>0.05).There were also no statistical differences in pharmacokinetic parameters between female and male rats.The elimination half-lifes(T1/2)of males and females were(3.02±0.68)h and(3.01±0.42)h in Con group,and(3.64±0.51)h and(3.38±0.60)h in IR group,respectively(P>0.05).Conclusion:A rapid and sensitive UPLC-MS/MS method for determination of E3 was established.The pharmacokinetic characteristics of E3 in rat were not affected by 7 Gy irradiation and gender differences.This study provided a theoretical basis for the development and application of new radiation injury treatment drug。

产前超声定量参数联合血清β-人绒毛膜促性腺激素、甲胎蛋白、游离雌三醇对胎儿颜面部畸形筛查具有较好价值

目的探讨产前超声定量联合血清β-人绒毛膜促性腺激素(β-HCG)、甲胎蛋白(AFP)、游离雌三醇(uE3)对胎儿颜面部畸形的筛查价值。方法选取2021年2月~2024年2月在我院进行产前筛查的123例孕中期孕妇为研究对象,均接受超声检查与血清指标检测,以引产或妊娠结果为“金标准”,分析产前超声定量参数、血清指标对胎儿颜面部畸形的诊断价值。结果123例孕妇最终发现胎儿颜面部畸形71例(57.72%),52例胎儿未出现颜面部畸形(42.28%)。单因素及多因素Logistic回归分析结果显示颈项透明层厚度(NT)、β-HCG、AFP及uE3水平均是胎儿颜面部畸形的危险因素(P<0.05)。超声NT诊出胎儿颜面部畸形61例,血清指标诊出胎儿颜面部畸形56例,联合检测诊出胎儿颜面部畸形67例,超声NT联合血清指标检测对胎儿颜面部畸形的诊断敏感度、特异度及准确度均高于单一检测,组间敏感度差异有统计学意义(P<0.05),但两组特异度、准确度的差异无统计学意义(P>0.05)。结论NT、β-HCG、AFP及uE3水平异常均提示胎儿颜面部畸形发生的高风险性。产期超声定量参数联合血清β-hCG、AFP、uE3可明显提高对胎儿颜面部畸形的检出敏感度,对产前颜面部畸形筛查有着较好的临床应用价值。

超声“四切面”法联合妊娠中期产妇血清Freeβ-hCG和uE3筛查胎儿严重先天性心脏病的研究

目的:探究超声“四切面”法联合妊娠中期孕妇血清游离人绒毛膜促性腺激素(Freeβ-hCG)和雌三醇(uE3)筛查胎儿严重先天性心脏病(CHD)的临床应用价值。方法:选择2020年1月到2021年6月我院行妊娠中期CHD筛查的孕妇97例,检测孕妇血清Freeβ-hCG和uE3水平,采用超声“四切面”法筛查CHD,分析超声“四切面”法联合血清Freeβ-hCG和uE3水平对CHD的诊断价值。结果:97例孕妇随访确诊为严重CHD 9例(9/97,9.28%);超声“四切面”法检查提示高危胎儿25例,其中4例确诊为严重CHD(4/25,16.00%);孕妇血清学检测提示高危胎儿36例,其中6例最终确诊为严重CHD(6/36,16.67%);超声“四切面”法联合孕妇血清学检测提示高危胎儿9例,其中5例确诊为严重CHD(5/9,55.56%)。超声“四切面”法筛查严重CHD的敏感度、特异度、准确度、阳性预测值和阴性预测值分别为16.00%、93.51%、74.51%、44.44%和77.22%。孕妇血清Freeβ-hCG和uE3水平筛查严重CHD的敏感度、特异度、准确度、阳性预测值和阴性预测值分别为55.56%、95.31%、92.08%、66.67%和64.00%,二者联合筛查严重CHD的敏感度、特异度、准确度、阳性预测值和阴性预测值分别为55.56%、95.65%、92.08%、55.56%和91.09%。二者联合检测的诊断情况高于单一检测方法(P<0.05)。结论:超声“四切面”法对妊娠中期孕妇筛查严重CHD具有较高的诊断价值,联合孕妇血清Freeβ-hCG和uE3水平能进一步提高诊断准确度,具有较好的临床应用价值。

同型半胱氨酸、叶酸、uE3诊断出生缺陷价值及与染色体异常的相关性

目的:探讨同型半胱氨酸(Hcy)、叶酸(FA)、游离雌三醇(uE3)对出生缺陷的诊断价值,并分析其与染色体异常的相关性。方法:选取2021年1月-2023年12月本院产前检查孕产妇临床资料,其中胎儿结构发育异常82例为出生缺陷组,正常产妇164例作为对照组。比较两组血清Hcy、FA、uE3水平,构建二元logistic回归模型,受试者工作特征(ROC)曲线分析3项指标诊断出生缺陷价值,分析出生缺陷组合并染色体异常孕妇血清Hcy、FA、uE3水平与染色体异常发生的相关性。结果:出生缺陷组血清Hcy(7.27±1.20μmlo/L)高于对照组(6.26±1.15μmlo/L),FA(27.78±2.61nmlo/L)、uE3(1.62±0.27MoM)低于对照组(30.77±2.85nmlo/L、1.85±0.17MoM)(均P<0.05)。二元logistic回归模型分析,出生缺陷计算公式=0.943Hcy-0.335×FA-3.040×uE3+8.039;ROC曲线分析,Hcy、FA、uE3及3项联合诊断出生缺陷的曲线下面积分别为0.747、0.779、0.862、0.878,敏感度分别为65.2%、65.2%、81.1%、90.2%,特异度分别为80.5%、78.1%、91.5%、84.5%。与未合并染色体异常异常孕妇比较,合并染色体异常孕妇血清Hcy水平更高,FA、uE3水平更低;经Spearman分析,胎儿结构发育异常孕妇Hcy与染色体异常成正相关,FA、uE3与染色体异常呈负相关(均P<0.05)。结论:血清Hcy、FA、uE3联合检测可提高出生缺陷诊断价值,且3项指标水平与染色体异常具有相关性。

TORCH联合血清β-hCG、AFP、uE3检测诊断超声软指标异常胎儿价值

目的:探究TORCH联合血清人绒膜促性腺激素(β-hCG)、甲胎蛋白(AFP)和游离雌三醇(uE3)检测对超声软指标异常胎儿产前诊断价值。方法:选取2021年7月-2023年12月于本院产前检查胎儿超声软指标异常的孕妇324例(300例单项软指标异常、24例≥2项软指标异常)纳入异常组,产前检查健康产妇300例纳入对照组。均行TORCH血清学检测,测定血清AFP、freeβ-hCG及uE3水平。对比两组血清TORCH、β-hCG、AFP和uE3检测结果;对比不同软指标异常组血清TORCH、β-hCG、AFP和uE3检测结果;ROC分析TORCH联合血清β-hCG、AFP和uE3对超声软指标异常胎儿产前诊断价值。结果:与对照组相比,异常组血清TORCH阳性率、β-hCG、AFP水平更高,血清uE3水平更低(均P<0.05);与单项软指标异常组相比,多项软指标异常组血清TORCH阳性率、β-hCG、AFP更高,血清uE3水平更低(均P<0.05);ROC工作曲线结果显示,血清TORCH、β-hCG、AFP和uE3联合检测诊断超声软指标异常孕妇的AUC分别为0.927,显著高于指标单独检测,且敏感度和特异度均较高,分别为90.00%和92.59%。结论:对产前检查孕妇行血清TORCH联合β-hCG、AFP和uE3检测,有助于提升超声软指标异常胎儿的产前诊断率,可为临床提供一种准确有效的诊断方法。

高危妊娠孕妇血清游离雌三醇联合胎儿大脑中动脉血流参数对母婴不良结局的预测价值

【目的】探讨高危妊娠孕妇血清游离雌三醇(uE3)联合胎儿大脑中动脉血流参数对母婴不良结局的预测价值。【方法】选取2019年2月至2021年2月在本院定期产检的95例高危妊娠孕妇(观察组),根据孕妇妊娠结局分为不良组和良好组;另选取同期在本院定期产检的88例健康孕妇作为对照组。比较各组孕妇临床资料、血清游离雌三醇(uE3)水平及胎儿大脑中动脉血流参数[大脑中动脉搏动指数(PI)、阻力指数(RI)、收缩期末峰值/舒张期末峰值(S/D)];采用Logistic回归分析高危妊娠孕妇发生不良妊娠结局的危险因素;采用受试者工作特征(ROC)曲线分析胎儿大脑中动脉血流参数和孕妇血清uE3水平对高危妊娠孕妇发生不良妊娠结局的预测价值。【结果】观察组孕妇血清uE3水平及胎儿大脑中动脉PI、RI、S/D均低于对照组(P<0.05)。高危妊娠孕妇不良妊娠结局发生率为35.79%。不良组孕妇血清uE3水平和胎儿大脑中动脉PI、RI、S/D均低于良好组(P<0.05);不良组孕妇流产史、胎盘前置构成比与良好组比较,差异均有统计学意义(P<0.05)。Logistic多因素回归分析结果显示:胎儿PI、RI、S/D低及孕妇血清uE3水平低、胎盘前置均是高危妊娠孕妇发生不良妊娠结局的独立危险因素(P<0.05);ROC曲线分析结果显示,胎儿大脑中动脉PI、RI、S/D和孕妇血清uE3水平预测高危妊娠孕妇发生不良妊娠结局的最佳截断点分别为0.92、0.42、3.20、0.66 ng/mL,曲线下面积(AUC)分别为0.797、0.756、0.708、0.766,联合预测的特异度和AUC分别为98.33%和0.838。【结论】不良妊娠结局孕妇胎儿大脑中动脉PI、RI、S/D及孕妇血清uE3水平降低,监测高危妊娠孕妇胎儿大脑中动脉PI、RI、S/D和孕妇血清uE3水平对不良妊娠结局具有一定的预测价值。

产前血清uE3、游离β-hCG、AFP水平与孕妇不良妊娠结局的相关性分析

目的 分析产前血清游离雌三醇(uE3)、游离人绒毛膜促性腺激素(β-hCG)、甲胎蛋白(AFP)水平与孕妇不良妊娠结局的相关性。方法 选择2022年1月至2023年1月本院200名孕妇,检测产前血清uE3、游离β-hCG、AFP水平,对患者进行随访,统计妊娠结局并据此分为两组,比较高风险妊娠结局组和低风险妊娠结局组孕妇的资料,分析产前血清uE3、游离β-hCG、AFP水平与孕妇高风险妊娠结局的相关性以及对高风险妊娠结局的评估价值。结果 高风险妊娠结局组uE3、AFP水平低于低风险妊娠结局组,游离β-hCG高于低风险妊娠结局组(均P <0.05);经Logistic回归分析结果显示,产前血清uE3、游离β-hCG、AFP水平与孕妇高风险妊娠结局发生有关(P <0.05);绘制ROC曲线图,结果显示,产前血清uE3、游离β-hCG、AFP水平评估孕妇高风险妊娠结局的AUC分别为0.729、0.753、0.765。结论 产前血清uE3、AFP低水平及游离β-hCG高水平与孕妇高风险妊娠结局有关,可作为预测因子预测不良妊娠结局的风险。

就地硼氢化法的改进——雌三醇合成工艺的研究

在前报中,我们应用外源性硼烷进行硼氢化反应,成功地改进了合成雌三醇(3)的工艺,收率高达85～95％;并以闪式柱层析分离出两个副产物:表雌三醇(4)和雌三醇-17-乙基醚(5):

雌三醇栓促进宫颈糜烂物理疗法后创面修复的多中心临床试验研究

目的 :观察宫颈糜烂物理疗法后应用雌三醇栓促进创面修复愈合的疗效。方法 :随机选取 10 6例宫颈糜烂中、重度患者 ,采用单纯物理治疗为对照组 (n=5 2 ) ,采用物理治疗后应用雌三醇栓为治疗组 (n=5 4) ,1天 1枚 ,10天 1疗程 ,比较两组疗效。结果 :治疗组创面修复时间为 3.82± 0 .0 7周 ,明显短于对照组 5 .95± 0 .71周 (P<0 .0 5 ) ;术后治疗组阴道出血发生率明显低于对照组 ,且以点状出血为主 ,无一例出血量超过既往月经量 ,出血时间为 7.42± 0 .5 5天 ,明显低于对照组的 17.5 1± 0 .34天 (P<0 .0 5 )。结论 :宫颈糜烂物理疗法后应用雌三醇 。

管式磁性微粒子化学发光免疫分析法测定人尿液中的雌三醇

待测尿液中的雌三醇、辣根过氧化物酶标记的雌三醇与异硫氰酸荧光素(FITC)标记的兔抗雌三醇抗体在均相体系中发生竞争性免疫反应,再加入用羊抗FITC抗体包被的磁微粒,反应生成物结合在磁微粒上,在磁场中经分离、洗涤后加发光底物,检测发光强度(RLU),测定尿液中雌三醇的含量。通过对检测条件的优化,建立了磁性微粒子化学发光免疫分析法测定人尿液中雌三醇的方法,并对正常男性、女性和孕妇的尿液中雌三醇含量进行了测定。结果表明,本方法的线性范围为1～100μg/L,检出限为0.25μg/L,具有很高的灵敏度;批内相对标准偏差<14%;批间相对标准偏差<7%,具有良好的稳定性和重现性。

高效液相色谱-串联质谱法测定海水中雌酮、雌二醇、雌三醇

建立了测定海水中雌酮、雌二醇和雌三醇的高效液相色谱-串联质谱的分析方法。样品的提取方法为固相萃取,流动相为乙腈和0.1%氨水,梯度洗脱,流速0.2 mL/min,运行时间10 min。质谱采用负离子扫描模式,定量的碎片离子分别是：雌酮：269.02/144.99;雌二醇：271.04/182.96;雌三醇：287.03/170.94。仪器检出限均为0.001 ng,方法检出限均为0.2 ng/L。回收率分别是78.0-110.0%,82.2-103.2%,76.4-95.1%。该方法适用于海水中雌激素类物质的检测。

毛细管电泳免疫分析法分析雌三醇

利用一种温敏水凝胶作为毛细管电泳的填充介质 ,建立了毛细管电泳免疫分析血清中雌三醇的方法。研究了缓冲溶液的浓度和pH值、水凝胶的浓度、电压等因素对分析结果的影响。雌三醇的检出限和线性范围分别为 3 1 .6ng L和 5 0～ 5 0 0 0ng L。

胶体金免疫试纸法快速测定雌三醇

雌三醇(E3)在临床诊断、日用化工和畜牧业等领域具有广泛应用,同时它也是一种环境污染物,对E3的检测具有科学和应用意义.本研究采用竞争性免疫层析技术,以E3单克隆抗体-纳米胶体金复合物作为金标抗体,以E3偶联抗原为检测线(T线)、以羊抗鼠抗抗体为控制线(C线),通过条件的优化,制备出检测E3的胶体金试纸条.当试纸条C线和T线同时显色时,结果为阴性;当试纸条仅C线显色时,结果为阳性.所得检测试纸条测加标水样的最低检测限为100μg/L,测加标奶样的最低检测限为100μg/L,反应时间为10min,与雌酮、雌二醇、己烷雌酚等环境雌激素无交叉反应,具有良好的选择性.

新型衍生化试剂雌激素衍生物的大气压化学电离质谱增敏研究

合成了用于酚羟基化合物衍生化试剂10-乙基吖啶酮-2-磺酰氯(EASC),EASC与雌二醇和雌三醇,在pH=10.5的NaHCO3缓冲溶液中、60℃下反应3min可获得稳定的衍生产物。EASC与丹磺酰氯(Dansyl-Cl)相比:最大摩尔吸光系数之比UVEASC/UVDansyl-Cl=6.67;荧光量子效率之比Φf(EASC)/Φf(Dansyl-Cl)=43,对雌二醇和雌三醇标记后的质谱离子流强度比为:ICEASC/ICDansyl-Cl=4.98(雌二醇)和ICEASC/ICDansyl-Cl=4.51(雌三醇)。在LC-MS-APCI(MRM)模式下,能够高灵敏地实现雌二醇和雌三醇的快速质谱测定。建立的方法具有良好的重现性,回归系数大于0.9995;检出限分别为4.3ng/L(雌二醇)和14ng/L(雌三醇)。对实际样品中藏羚羊粪便、根田鼠尿样和狼血清中痕量游离的雌二醇和雌三醇进行了测定,结果满意。

人血清中雌三醇的磁性微粒子放射免疫分析法研究

在传统的放射免疫分析的基础上，以磁性微粒子为二抗分离剂和固相一抗为结合反应剂，建立了两种快速、超灵敏、简便的血清及其它样品中雌三醇含量的分析方法。雌三醇测定的曲线范围0．3～30μg／L；磁性微粒子二抗分离法批内和批间相对标准偏差分别为4．3％和8．7％；回收率为104％～114％；健全性为0．9989。磁性微粒子固相一抗法批内和批间相对标准偏差分别为4．4％和7．6％；回收率为96％～115％；健全性为0．9986。两种方法相比，磁性微粒子固相一抗法具有更为简便、快捷的优点。两种磁性微粒子的放射免疫分析不仅缩短了反应时间，其准确性和精密度也均满足临床及环境研究中的检验要求，且灵敏度较现有方法提高了一个数量级。